Recombinant Human FGF basic/FGF2/bFGF Protein

SDS-Page: Human FGF basic/FGF2 Protein [NBP2-34921]

• Reactivity: Hu
• Applications: Func, PAGE, Bioactivity
• Format: Carrier-Free
• Concentration: LYOPH

Recombinant Human FGF basic/FGF2/bFGF Protein Summary

• Description: A single, non-glycosylated polypeptide chain corresponding to 147 residues of FGF basic/FGF2/bFGF.
  
  

  Source: Escherichia coli

  Amino Acid Sequence: MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS

• Preparation Method: Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance.
• Details of Functionality: Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x 10^7 IU/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: FGF2
• Purity: >96%, by HPLC
• Endotoxin Note: Less than 1 EU/ug of FGF basic/FGF2/bFGF as determined by LAL method.

Applications/Dilutions

• Theoretical MW: 16.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 7.6, with 150mM NaCl.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >96%, by HPLC
• Reconstitution Instructions: Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of less than 0.3mg/mL. Apportion stock solutions into working aliquots and store at <-20C.

Notes

This lyophilized preparation is stable for 12 months from date of receipt at -20 to -70 degrees C, preferably desiccated. Upon reconstitution, the preparation can be stored for 1 month at 2-8 degrees C under sterile conditions, and for 3 months at -20 degrees C to -70 degrees C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Stock solutions should be aliquoted and stored at < -20 degrees C. Further dilutions should be made in appropriate buffered solutions

Alternate Names for Recombinant Human FGF basic/FGF2/bFGF Protein

• basic fibroblast growth factor bFGF
• Basic fibroblast growth factor
• bFGF
• FGF basic
• FGF2
• FGF-2
• FGFBprostatropin
• fibroblast growth factor 2 (basic)
• HBGF-2
• heparin-binding growth factor 2
• Prostatropin

Background

Human FGF-2 (bFGF), encoded by the FGF2 gene, is a member of the fibroblast growth factor (FGF) family. Fibroblast growth factor was found in pituitary extracts in 1973 and then tested in a bioassay that caused fibroblasts to proliferate. After further fractionating the extract using acidic and basic pH, two different forms have isolated that named "acidic fibroblast growth factor" (FGF-1) and "basic fibroblast growth factor" (FGF-2). Human FGF-2 (bFGF) shares 54 % amino acid sequence identity with aFGF. Affinity between FGF-2 (bFGF) and its receptors can be increased by heparin or heparan sulfate proteoglycan. FGF-2 (bFGF) plays an important role in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. FGF-2 (bFGF) are also involved in a variety of biological processes, including embryonic development, morphogenesis, tissue repair, tumor growth and invasion. Additionally, FGF-2 (bFGF) is frequently used for a critical component of cell culture medium, e.g., human embryonic stem cell culture medium, serum-free culture systems.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for FGF basic/FGF2/bFGF Recombinant Protein (NBP2-34921)(6)

Publications using NBP2-34921

• Li X, Wang Z, Jiang Z et al. Regulation of seminiferous tubule-associated stem Leydig cells in adult rat testes. Proc. Natl. Acad. Sci. U.S.A. Mar 8 2016 [PMID: 26929346]
• Chao Feng, Jie Li, Jinlan Ruan, Kan Ding. MicroRNA-125a inhibits cell growth by targeting glypican-4. Glycoconj J 2012 Oct [PMID: 22644326]
• Li Y, IbaNez D, Fan W et al. Generation of an induced pluripotent stem cell line (GIBHi004-A) from a Parkinson's disease patient with mutant DJ-1/PARK7 (p.L10P) Stem Cell Research May 1 2020 [PMID: 32534165] (Human)
• Abdul M, IbaNez D, Zhao P et al. Generation of an induced pluripotent stem cell line (GIBHi003-A) from a Parkinson's disease patient with mutant PINK1 (p. I368N) Stem Cell Research 2019 Oct 01 [PMID: 31778937]
• Nikolaou S, Hadjikypri X, Ioannou G et al. Functional and phenotypic distinction of the first two trophoblast subdivisions and identification of the border between them during early postimplantation: A prerequisite for understanding early patterning during placentogenesis. Biochem Biophys Res Commun 2018 Jan 29 [PMID: 29305264]

Reviews for FGF basic/FGF2/bFGF Recombinant Protein (NBP2-34921) (2)

Average Rating: 5

(Based on 2 reviews)

reviewed by: Anonymous 01/04/2017

Summary

• Comments: I ranked this protein with 5 stars because the cell differentiation was successfully performed.

Comments

• Comments: SDS PAGE gel with Coomassie blue staining showing detection of Human FGF basic/FGF 2 Protein ( NBP2-34921) (0.5 ug) at about 17 kDa

reviewed by: Anonymous 01/04/2017

Summary

• Comments: I ranked this protein with 5 stars because the cell differentiation was successfully performed.

Comments

• Comments: SDS PAGE gel with Coomassie blue staining showing detection of 0.5 ug Human FGF basic protein (NBP2-34921-10 ug)

FAQs for FGF basic/FGF2/bFGF Recombinant Protein (NBP2-34921). (Showing 1 - 1 of 1 FAQs).

1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
   • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have two antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). Here is a link to those FGF-2 antibodies. We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits, but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we

Bioinformatics

• Gene Symbol: FGF2
• Uniprot:
  • P09038()
  • P09038()
  • P09038()