Reactivity | HuSpecies Glossary |
Applications | PAGE, Bioactivity |
Format | Carrier-Free |
Concentration | LYOPH |
Description | A single non-glycosylated polypeptide chain containing 147 amino acids corresponding to FGF basic/FGF2/bFGF Source: E. coli Uniprot ID: P09038 Amino Acid Sequence: MPALPEDGGS GAFPPGHFKD PKRLYCKNGG FFLRIHPDGR VDGVREKSDP HIKLQLQAEE RGVVSIKGVC ANRYLAMKED GRLLASKCVT DECFFFERLE SNNYNTYRSR KYTSWYVALK RTGQYKLGSK TGPGQKAILF LPMSAKS |
Preparation Method |
Novus' biologically active proteins are stringently purified to provide only the safest and most highly effective proteins available. This protein was expressed in E. coli, purified by HPLC, QC tested by SDS-PAGE and Western Blot and validated on appropriate cell lines for bioactivity. All HPLC and bioactivity data is provided for your assurance. |
Details of Functionality | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 0.05 ng/ml, corresponding to a specific activity of > 2.0 x 10^7 IU/mg. |
Source | E. coli |
Protein/Peptide Type | Recombinant Protein |
Gene | FGF2 |
Purity | >95%, by SDS-PAGE and HPLC |
Endotoxin Note | Less than 1 EU/ug of FGF basic/FGF2/bFGF as determined by LAL method. |
Dilutions |
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Theoretical MW | 16.5 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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Storage | Store at -20 to -70C as supplied. After reconstitution, store at 2 to 8C for 1 month and at -20 to -70C for long term storage. Avoid repeated freeze-thaw cycles. |
Buffer | Lyophilized from a 0.2 um filtered concentrated solution in 20 mM Tris-HCl, pH 7.6, with 150mM NaCl. |
Preservative | No Preservative |
Concentration | LYOPH |
Purity | >95%, by SDS-PAGE and HPLC |
Reconstitution Instructions | Recommended to centrifuge prior to opening. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of less than 0.3mg/mL. Apportion stock solutions into working aliquots and store at <-20C. |
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reviewed by:
L P |
SDS-PAGE Control | 01/04/2017 |
Summary
Comments
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reviewed by:
L P |
SDS-PAGE Control | 01/04/2017 |
Summary
Comments
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Diseases for FGF basic/FGF2/bFGF Recombinant Protein (NBP2-34921)Discover more about diseases related to FGF basic/FGF2/bFGF Recombinant Protein (NBP2-34921).
| Pathways for FGF basic/FGF2/bFGF Recombinant Protein (NBP2-34921)View related products by pathway.
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Application: | SDS-PAGE Control | |
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L P 01/04/2017 |
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Application: | SDS-PAGE Control | |
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