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85 Results for "fgf-basic-fgf2-bfgf" in Primary Antibodies
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AF-233-NA

FGF basic/FGF2/bFGF Antibody [Unconjugated]

(28 Publications)
100 μg
AF-233-NA
FGF basic/FGF2/bFGF was detected in immersion fixed paraffin-embedded sections of human placenta using Goat Anti-Human FGF basic/FGF2/bFGF Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-233-NA) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to trophoblast cells in chorionic villi. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Hu
Species Glossary
Reactivity: Hu
WB, IHC, B/N
Applications: WB, IHC, B/N
Goat Polyclonal
Host: Goat Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 AF-233-NAU-100UG
Alexa Fluor 405 AF-233-NAV-100UG
Alexa Fluor 488 AF-233-NAG-100UG
Alexa Fluor 532 AF-233-NAX-100UG
Alexa Fluor 594 AF-233-NAT-100UG
Alexa Fluor 647 AF-233-NAR-100UG
Alexa Fluor 700 AF-233-NAN-100UG
Alexa Fluor 750 AF-233-NAS-100UG
Allophycocyanin AF-233-NAA
Biotin BAF233
CoraFluor 1 AF-233-NACL1
DyLight 350 AF-233-NAD
DyLight 405 AF-233-NAE
DyLight 488 AF-233-NAK
DyLight 550 AF-233-NAL
DyLight 594 AF-233-NAM
DyLight 650 AF-233-NAW
DyLight 680 AF-233-NAY
DyLight 755 AF-233-NAZ
FITC AF-233-NAF
HRP AF-233-NAH
Janelia Fluor 525 AF-233-NAJF525
Janelia Fluor 549 AF-233-NAI
Janelia Fluor 646 AF-233-NAJ
Janelia Fluor 669 AF-233-NAJF669
PE AF-233-NAP
PerCP AF-233-NAC
mFluor Violet 450 SE AF-233-NAMFV450
mFluor Violet 500 SE AF-233-NAMFV500
mFluor Violet 610 SE AF-233-NAMFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free AF-233-SP
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AB-33-NA

FGF basic/FGF2/bFGF Antibody [Unconjugated]

(5 Publications)
1 mg
AB-33-NA
Bovine FGF basic/FGF2/bFGF (Catalog # 133-FB) stimulates proliferation in the NR6R‑3T3 mouse fibroblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Bovine FGF basic/FGF2/bFGF/bFGF (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Rabbit Anti-Bovine FGF basic/FGF2/bFGF Polyclonal Antibody (Catalog # AB-33-NA). The ND50 is typically 1.5‑4.5 µg/mL.
Bv
Species Glossary
Reactivity: Bv
WB, B/N
Applications: WB, B/N
Rabbit Polyclonal
Host: Rabbit Polyclonal
Unconjugated  
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NBP1-47749

FGF basic/FGF2/bFGF Antibody (OTI3D9)

(2 Publications)
0.1 ml
NBP1-47749
Western Blot: FGF basic/FGF2 Antibody (3D9) [NBP1-47749] - Analysis of extracts (10ug) from 4 different cell lines by using anti-BFGF monoclonal antibody at 1:200 dilution
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB, ICC/IF, IHC
Applications: WB, ICC/IF, IHC
Mouse Monoclonal
Host: Mouse Monoclonal
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 NBP2-70718AF350
Alexa Fluor 405 NBP2-70718AF405
Alexa Fluor 488 NBP2-70718AF488
Alexa Fluor 532 NBP2-70718AF532
Alexa Fluor 594 NBP2-70718AF594
Alexa Fluor 647 NBP2-70718AF647
Alexa Fluor 700 NBP2-70718AF700
Alexa Fluor 750 NBP2-70718AF750
Alexa Fluor Plus 488 NBP2-70718AFP488
Alexa Fluor Plus 555 NBP2-70718AFP555
Alexa Fluor Plus 594 NBP2-70718AFP594
Alexa Fluor Plus 647 NBP2-70718AFP647
Alexa Fluor Plus 680 NBP2-70718AFP680
Allophycocyanin NBP2-70718APC
Biotin NBP2-70718B
CoraFluor 1 NBP2-70718CL1
DyLight 350 NBP2-70718UV
DyLight 405 NBP2-70718V
DyLight 488 NBP2-70718G
DyLight 550 NBP2-70718R
DyLight 594 NBP2-70718DL594
DyLight 650 NBP2-70718C
DyLight 680 NBP2-70718FR
DyLight 755 NBP2-70718IR
FITC NBP2-70718F
HRP NBP2-70718H
Janelia Fluor 525 NBP2-70718JF525
Janelia Fluor 549 NBP2-70718JF549
Janelia Fluor 646 NBP2-70718JF646
Janelia Fluor 669 NBP2-70718JF669
PE NBP2-70718PE
PerCP NBP2-70718PCP
mFluor Violet 450 SE NBP2-70718MFV450
mFluor Violet 500 SE NBP2-70718MFV500
mFluor Violet 610 SE NBP2-70718MFV610
Available Formulations
Formulation Catalog # Availability Price  
Azide and BSA Free NBP2-70718
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NBP3-15367

FGF basic/FGF2/bFGF Antibody (8W6M5)

100 ul
NBP3-15367
Western Blot: FGF basic/FGF2/bFGF Antibody (8W6M5) [NBP3-15367] - Analysis of extracts of SKOV3 cells, using FGF basic/FGF2/bFGF Rabbit mAb (NBP3-15367) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
Hu
Species Glossary
Reactivity: Hu
WB, ELISA, ICC/IF, IHC
Applications: WB, ELISA, ICC/IF, IHC
Rabbit Monoclonal
Host: Rabbit Monoclonal
Unconjugated  
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NB600-1536

FGF basic/FGF2/bFGF Antibody - Azide and BSA Free

(3 Publications)
0.1 ml
NB600-1536
High VEGF-A and FGF2 labeling levels in an orthotopic OS mouse model and in human lung metastasis specimens: (A) U2-OS tumor image at 5 weeks and (B) anti-VEGF-A immunolabeling. Representative images of primary OS tumors in mice and nearby bone. The labeling pattern was mainly cytoplasmic; however, nuclear labeling was also noticed. Images were obtained under a ×40 magnification. Scale bar denotes 50 um. (C) Quantitative analysis of immunolabeled area (tumor/bone ratio) of primary OS tumors in mice normalized to nearby bone. Primary OS tumors exhibit significantly higher VEGF-A levels compared to control bone; *** p < 0.001. (D) FGF2 immunolabeling. Representative images of primary OS tumors in mice and nearby bone. Images were obtained under ×40 magnification. Scale bar denotes 50 um. (E) Quantitative analysis of immunolabeled area (tumor/bone ratio) of primary OS tumors in mice normalized to nearby bone. Primary OS tumors exhibit significantly higher FGF2 levels compared to control bone; ** p < 0.01. (F) Representative anti-VEGF-A and anti-FGF2 immunolabeling of human metastatic OS and non-metastatic OS patient specimens. Images showing nuclear labeling pattern. Microscope images were obtained under ×40 magnification. Scale bar denotes 50 um. (G) Quantitative analysis of immunolabeled area (1/um2) in metastatic vs. non-metastatic human OS specimens. Higher levels of VEGF-A (primary, N = 7; metastasis, N = 8) and FGF2 (primary, N = 6; metastasis, N = 14) were obtained in metastatic samples compared to primary tumors; * p < 0.05, and **** p < 0.0001. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/2072-6694/15/6/1818), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Hu, Rt, Bv, Eq
Species Glossary
Reactivity: Hu, Rt, Bv, Eq
WB, ELISA, IHC
Applications: WB, ELISA, IHC
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor Plus 488 NB600-1536AFP488
Alexa Fluor Plus 555 NB600-1536AFP555
Alexa Fluor Plus 594 NB600-1536AFP594
Alexa Fluor Plus 647 NB600-1536AFP647
Alexa Fluor Plus 680 NB600-1536AFP680
Available Formulations
Formulation Catalog # Availability Price  
Azide and BSA Free NB600-1536-0.025ml
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MAB2332

FGF basic/FGF2/bFGF Antibody (954824) [Unconjugated]

100 μg
MAB2332
FGF basic/FGF2/bFGF was detected in immersion fixed paraffin-embedded sections of Liver using Rat Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody (Catalog # MAB2332) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Rat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC005). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell nuclei. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Hu
Species Glossary
Reactivity: Hu
IHC, ICC/IF
Applications: IHC, ICC/IF
Rat Monoclonal
Host: Rat Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB2332U-100UG
Alexa Fluor 405 FAB2332V-100UG
Alexa Fluor 488 FAB2332G-100UG
Alexa Fluor 532 FAB2332X-100UG
Alexa Fluor 594 FAB2332T-100UG
Alexa Fluor 647 FAB2332R-100UG
Alexa Fluor 700 FAB2332N-100UG
Alexa Fluor 750 FAB2332S-100UG
Allophycocyanin FAB2332A
Biotin FAB2332B
CoraFluor 1 FAB2332CL1
DyLight 350 FAB2332D
DyLight 405 FAB2332E
DyLight 488 FAB2332K
DyLight 550 FAB2332L
DyLight 594 FAB2332M
DyLight 650 FAB2332W
DyLight 680 FAB2332Y
DyLight 755 FAB2332Z
FITC FAB2332F
HRP FAB2332H
Janelia Fluor 525 FAB2332JF525
Janelia Fluor 549 FAB2332I
Janelia Fluor 646 FAB2332J
Janelia Fluor 669 FAB2332JF669
PE FAB2332P
PerCP FAB2332C
mFluor Violet 450 SE FAB2332MFV450
mFluor Violet 500 SE FAB2332MFV500
mFluor Violet 610 SE FAB2332MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB2332-SP
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MAB11377

FGF basic/FGF2/bFGF Antibody (10056) [Unconjugated]

100 μg
MAB11377
FGF basic/FGF2/bFGF was detected in immersion fixed paraffin-embedded sections of Human Liver Cancer using Mouse Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody (Catalog # MAB11377) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm in hepatocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Hu
Species Glossary
Reactivity: Hu
IHC
Applications: IHC
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB11377U-100UG
Alexa Fluor 405 FAB11377V-100UG
Alexa Fluor 488 FAB11377G-100UG
Alexa Fluor 532 FAB11377X-100UG
Alexa Fluor 594 FAB11377T-100UG
Alexa Fluor 647 FAB11377R-100UG
Alexa Fluor 700 FAB11377N-100UG
Alexa Fluor 750 FAB11377S-100UG
Allophycocyanin FAB11377A
Biotin FAB11377B
CoraFluor 1 FAB11377CL1
DyLight 350 FAB11377D
DyLight 405 FAB11377E
DyLight 488 FAB11377K
DyLight 550 FAB11377L
DyLight 594 FAB11377M
DyLight 650 FAB11377W
DyLight 680 FAB11377Y
DyLight 755 FAB11377Z
FITC FAB11377F
HRP FAB11377H
Janelia Fluor 525 FAB11377JF525
Janelia Fluor 549 FAB11377I
Janelia Fluor 646 FAB11377J
Janelia Fluor 669 FAB11377JF669
PE FAB11377P
PerCP FAB11377C
mFluor Violet 450 SE FAB11377MFV450
mFluor Violet 500 SE FAB11377MFV500
mFluor Violet 610 SE FAB11377MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB11377-SP
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MAB233R

FGF basic/FGF2/bFGF Antibody (10060R) [Unconjugated]

100 μg
Hu
Species Glossary
Reactivity: Hu
ELISA(Cap)
Applications: ELISA(Cap)
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB233RU-100UG
Alexa Fluor 405 FAB233RV-100UG
Alexa Fluor 488 FAB233RG-100UG
Alexa Fluor 532 FAB233RX-100UG
Alexa Fluor 594 FAB233RT-100UG
Alexa Fluor 647 FAB233RR-100UG
Alexa Fluor 700 FAB233RN-100UG
Alexa Fluor 750 FAB233RS-100UG
Allophycocyanin FAB233RA
Biotin FAB233RB
CoraFluor 1 FAB233RCL1
DyLight 350 FAB233RD
DyLight 405 FAB233RE
DyLight 488 FAB233RK
DyLight 550 FAB233RL
DyLight 594 FAB233RM
DyLight 650 FAB233RW
DyLight 680 FAB233RY
DyLight 755 FAB233RZ
FITC FAB233RF
HRP FAB233RH
Janelia Fluor 525 FAB233RJF525
Janelia Fluor 549 FAB233RI
Janelia Fluor 646 FAB233RJ
Janelia Fluor 669 FAB233RJF669
PE FAB233RP
PerCP FAB233RC
mFluor Violet 450 SE FAB233RMFV450
mFluor Violet 500 SE FAB233RMFV500
mFluor Violet 610 SE FAB233RMFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB233R-SP
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MAB2331

FGF basic/FGF2/bFGF Antibody (10043R) [Unconjugated]

100 μg
MAB2331
Recombinant Human FGF basic/FGF2/bFGF protein was serially diluted 2-fold and captured by Mouse Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody(Catalog # MAB233R) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody (Catalog # MAB2331) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Hu
Species Glossary
Reactivity: Hu
ELISA
Applications: ELISA
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB2331U-100UG
Alexa Fluor 405 FAB2331V-100UG
Alexa Fluor 488 FAB2331G-100UG
Alexa Fluor 532 FAB2331X-100UG
Alexa Fluor 594 FAB2331T-100UG
Alexa Fluor 647 FAB2331R-100UG
Alexa Fluor 700 FAB2331N-100UG
Alexa Fluor 750 FAB2331S-100UG
Allophycocyanin FAB2331A
Biotin FAB2331B
CoraFluor 1 FAB2331CL1
DyLight 350 FAB2331D
DyLight 405 FAB2331E
DyLight 488 FAB2331K
DyLight 550 FAB2331L
DyLight 594 FAB2331M
DyLight 650 FAB2331W
DyLight 680 FAB2331Y
DyLight 755 FAB2331Z
FITC FAB2331F
HRP FAB2331H
Janelia Fluor 525 FAB2331JF525
Janelia Fluor 549 FAB2331I
Janelia Fluor 646 FAB2331J
Janelia Fluor 669 FAB2331JF669
PE FAB2331P
PerCP FAB2331C
mFluor Violet 450 SE FAB2331MFV450
mFluor Violet 500 SE FAB2331MFV500
mFluor Violet 610 SE FAB2331MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB2331-SP
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MAB2333

FGF basic/FGF2/bFGF Antibody (10044) [Unconjugated]

100 μg
MAB2333
FGF basic/FGF2/bFGF was detected in immersion fixed A172 human glioblastoma cells (positive) and absent in Daudi human Burkitt's lymphoma cells (negative) using Mouse Anti-Human FGF basic/FGF2/bFGF Monoclonal Antibody (Catalog # MAB2333) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Hu
Species Glossary
Reactivity: Hu
ICC/IF
Applications: ICC/IF
Mouse Monoclonal
Host: Mouse Monoclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Conjugates:
Conjugate Catalog # Availability Size Price
Alexa Fluor 350 FAB2333U-100UG
Alexa Fluor 405 FAB2333V-100UG
Alexa Fluor 488 FAB2333G-100UG
Alexa Fluor 532 FAB2333X-100UG
Alexa Fluor 594 FAB2333T-100UG
Alexa Fluor 647 FAB2333R-100UG
Alexa Fluor 700 FAB2333N-100UG
Alexa Fluor 750 FAB2333S-100UG
Allophycocyanin FAB2333A
Biotin FAB2333B
CoraFluor 1 FAB2333CL1
DyLight 350 FAB2333D
DyLight 405 FAB2333E
DyLight 488 FAB2333K
DyLight 550 FAB2333L
DyLight 594 FAB2333M
DyLight 650 FAB2333W
DyLight 680 FAB2333Y
DyLight 755 FAB2333Z
FITC FAB2333F
HRP FAB2333H
Janelia Fluor 525 FAB2333JF525
Janelia Fluor 549 FAB2333I
Janelia Fluor 646 FAB2333J
Janelia Fluor 669 FAB2333JF669
PE FAB2333P
PerCP FAB2333C
mFluor Violet 450 SE FAB2333MFV450
mFluor Violet 500 SE FAB2333MFV500
mFluor Violet 610 SE FAB2333MFV610
Available Formulations
Formulation Catalog # Availability Price  
Carrier Free MAB2333-SP
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NBP1-57096

FGF basic/FGF2/bFGF Antibody - BSA Free

100 ul
NBP1-57096
Western Blot: FGF basic/FGF2 Antibody [NBP1-57096] - Antibody Titration: 2 ug/ml ELISA Titer: 1 : 312500 Positive control: Hela cell lysate.
Hu, Mu
Species Glossary
Reactivity: Hu, Mu
WB, IHC
Applications: WB, IHC
Rabbit Polyclonal
Host: Rabbit Polyclonal
Unconjugated  
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NBP1-18579

FGF basic/FGF2/bFGF Antibody - Azide and BSA Free

(2 Publications)
0.2 mg
NBP1-18579
Western Blot: FGF basic/FGF2 Antibody [NBP1-18579] - Analysis of recombinant human, mouse and rat FGF-2 (basic) using a polyclonal antibody directed against recombinant human FGF-2. As expected the antibody cross reacts with the mouse and rat proteins.
Hu, Mu, Rt
Species Glossary
Reactivity: Hu, Mu, Rt
WB
Applications: WB
Rabbit Polyclonal
Host: Rabbit Polyclonal
Sample Size Available
Sizes: Sample Size Available
Unconjugated  
Available Formulations
Formulation Catalog # Availability Price  
Azide and BSA Free NBP1-18579-0.025mg
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