FGF basic/FGF2 Antibody

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Immunohistochemistry: FGF basic/FGF2 Antibody [NB600-1536] - Immunoperoxidase staining (PAP) of U87MG glioma cell suspension implanted into nude rat brain (Han rnu/rnu) using Rabbit Anti-Fibroblast Growth Factor-Basic ...read more
Immunohistochemistry: FGF basic/FGF2 Antibody [NB600-1536] - Immunoperoxidase staining (PAP) of a fibrillary differentiated astrocytoma (WHO grade I, SAMS grade 1) using Rabbit Anti-Fibroblast Growth Factor-Basic. High ...read more
Immunohistochemistry: FGF basic/FGF2 Antibody [NB600-1536] - Immunoperoxidase staining (PAP) of a gemistocytic differentiated area of an anaplastic astrocytoma (WHO grade III, SAMS grade 3) using Rabbit Anti-Fibroblast ...read more
Immunohistochemistry: FGF basic/FGF2 Antibody [NB600-1536] - Immunoperoxidase staining (PAP) of a freshly explanted gliobastoma multiforme (WHO grade IV, SAMS grade 4) in culture (passage # 3) using Rabbit ...read more

Product Details

Summary
Reactivity Hu, Rt, Bv, EqSpecies Glossary
Applications WB, ELISA, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

FGF basic/FGF2 Antibody Summary

Immunogen
Synthetic peptide conjugated to KLH, corresponding to amino acids 1-24 of Cow FGF2.
Specificity
Does not cross react with bovine FGF Acidic or bovine serum albumin.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FGF2
Purity
Sterile filtered
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • ELISA indirect
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This antibody is useful for Western Blot and Immunohistochemistry-Paraffin. WB: Predicted molecular weight: 18 kDa.
Publications
Read Publications using
NB600-1536 in the following applications:

Reactivity Notes

Horse reactivity reported in scientific literature (PMID: 24035195).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.2 um filtered solution in PBS
Preservative
No Preservative
Concentration
LYOPH
Purity
Sterile filtered
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Alternate Names for FGF basic/FGF2 Antibody

  • basic fibroblast growth factor bFGF
  • Basic fibroblast growth factor
  • bFGF
  • FGF basic
  • FGF2
  • FGF-2
  • FGF2AS
  • FGFBprostatropin
  • fibroblast growth factor 2 (basic)
  • GFG1
  • HBGF-2
  • HBGH-2
  • heparin-binding growth factor 2
  • NUDT6
  • Prostatropin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Species: Hu, Rt, Bv, Eq
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Publications for FGF basic/FGF2 Antibody (NB600-1536)(2)

We have publications tested in 2 confirmed species: Rat, Horse.

We have publications tested in 2 applications: ELISA, IHC-P.


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ELISA
(1)
IHC-P
(1)
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(1)
Horse
(1)
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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for FGF basic/FGF2 Antibody (NB600-1536). (Showing 1 - 2 of 2 FAQs).

  1. I am looking for an ovine antibody for bFGF and PDGF-AB and ELISA Kits for both. It is for a research work on meniscus healing in sheep. Do you have anything suitable? How is the homology with sheep? Is it ever tested and/or published in papers?
    • Unfortunately, most of the products you are interested in have not yet been tested in sheep, but this should not be a problem, due to sequence homology, and our Innovators Reward Program. In terms of an antibody for bFGF, also called FGF-2, we have two antibodies that are validated to detect the bovine protein, which is almost identical to that from sheep (99% homology). Here is a link to those FGF-2 antibodies. We only have FGF2 ELISA kits that have been tested with human or mouse. Mouse FGF-2 has 94% homology with sheep; human has 98% homology for most of its sequence, but also has an additional stretch of amino acids that is not present in the sheep protein. I therefore think that the mouse kit would be most suitable, just in case the human kit targets the region of the human protein not present in mouse. We sell antibodies to PDGF-A or PDGF-B rather than PDGF-AB. We don't have any PDGF-A antibodies that have been tested in sheep but there are several options you may find of interest. There isn't a sheep PDGFA sequence in UniProt, so I can't comment on how similar it is to the PDGFA from other species. Nevertheless, our NBP1-52533 PDGFB antibody has been shown to work in sheep. We sell two PDGF-AB ELISA kits, but these are for human and rat. I'm not sure how well these would work with sheep. Note that if you test a product with a species (or application) that it has not yet been validated in, you are eligible for our Innovator's Reward: Novus would provide you a 50% refund on the purchased product as well as a 50% discount on a future product of equal or lesser value. You would email innovators@novusbio.com to apply for your award, and we
  2. What is the catalog of the blocking peptide or protein for this antibody? Or How can I find the blocking peptide or protein for this antibody?
    • Unfortunately we do not have a blocking peptide for this antibody in our catalogue. I am very sorry that we do not have what you are looking for. If you are experiencing non specific bands I would recommend blocking for longer with BSA.

Secondary Antibodies

 

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Bioinformatics

Gene Symbol FGF2
Entrez
Uniprot