FGF-4 Antibody - BSA Free

Western Blot: FGF-4 Antibody [NBP1-76865] - 3T3 cell lysate with FGF4 antibody at (A) 0.5 and (B) 1 ug/mL.

Immunocytochemistry/ Immunofluorescence: FGF-4 Antibody - BSA Free [NBP1-76865] - Immunofluorescence of FGF-4 in 3T3 cells with FGF-4 antibody at 2.5 ug/mL.

Immunocytochemistry/ Immunofluorescence: FGF-4 Antibody - BSA Free [NBP1-76865] - Immunocytochemistry of FGF-4 in 3T3 cells with FGF-4 antibody at 2.5 ug/mL.

• Reactivity: Hu, Mu, Bv
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

FGF-4 Antibody - BSA Free Summary

• Immunogen: Antibody was raised against a 18 amino acid synthetic peptide near the carboxy terminus of the human FGF4. The immunogen is located within the last 50 amino acids of FGF4. Amino Acid Squence: SKNGKTKKGNRVSPTMK
• Predicted Species: Bovine (94%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FGF4
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 2.5 ug/ml
  • Western Blot 0.5-1 ug/ml
• Control Peptide:

  FGF-4 Peptide (NBP1-76865PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Chicken (88%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for FGF-4 Antibody - BSA Free

• FGF4
• FGF-4
• fibroblast growth factor 4
• HBGF-4
• HBGF-4Transforming protein KS3
• heparin secretory transforming protein 1
• Heparin secretory-transforming protein 1
• Heparin-binding growth factor 4
• HST-1
• HST-1HSTF-1
• HSTF1fibroblast growth factor 4 splice isoform
• HSTFGF-4
• human stomach cancer, transforming factor from FGF-related oncogene
• kaposi sarcoma oncogene
• KFGF
• K-FGF
• KS3
• oncogene HST

Background

Fibroblast growth factor 4 (FGF4) is a member of the fibroblast growth factor (FGF) family that possess broad mitogenic and cell survival activities and play key roles in growth and survival of stem cells during embryogenesis, tissue regeneration, and carcinogenesis. FGF4 was identified by its strong oncogenic transforming activity and is a potent angiogenic factor, expressed in several highly vascularized tumors and also in adult mouse testis, intestine, and brain. Studies on the mouse homolog suggests a function in bone morphogenesis and limb development through the sonic hedgehog (SHH) signaling pathway. Furthermore, FGF4 regulates neural progenitor cell proliferation and neuronal differentiation. Recent studies show a growth-promoting role for FGF4 in human embryonic stem cells and a putative feedback inhibition mechanism by a novel FGF4 splice isoform that may serve to promote differentiation at a later stages of development.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: FGF4
• Uniprot:
  • P08620()