Factor IX Antibody

Images

 
Immunocytochemistry/ Immunofluorescence: Factor IX Antibody [NBP1-33581] - HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: Factor IX protein stained by Factor IX antibody [N3C3] diluted at 1:500. ...read more
Immunohistochemistry-Paraffin: Factor IX Antibody [NBP1-33581] - Paraffin-embedded rat liver. Factor IX antibody [N3C3] diluted at 1:500.
Immunohistochemistry-Paraffin: Factor IX Antibody [NBP1-33581] - Paraffin-embedded human colon cancer. Factor IX antibody [N3C3] diluted at 1:250.

Product Details

Summary
Reactivity Hu, Rt, Mk, PmSpecies Glossary
Applications ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.45 mg/ml

Order Details

Factor IX Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human Factor IX. The exact sequence is proprietary.
Localization
Secreted
Predicted Species
Monkey (96%), Primate (99%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
F9
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
52 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Expected cross reactivity based on sequence homology: Mouse (80%), Canine (87%), Porcine (87%), Rabbit (82%), Bovine (82%), Feline (83%), Rhesus Monkey (96%), Chimpanzee (99%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0) and 20% Glycerol
Preservative
0.025% Proclin 300
Concentration
1.45 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Factor IX Antibody

  • Christmas Factor
  • Coagulation Factor IX
  • EC 3.4.21
  • EC 3.4.21.22
  • F9
  • factor 9
  • FIX
  • HEMB
  • MGC129641
  • MGC129642
  • P19
  • Plasma Thromboplastic Component
  • Plasma thromboplastin component
  • PTC
  • THPH8

Background

This gene encodes vitamin K-dependent coagulation factor IX that circulates in the blood as an inactive zymogen. Coagulation factor IX is a single chain polypeptide. This factor is converted to an active form by factor XIa, which excises the activation peptide and thus generates a heavy chain and a light chain held together by one or more disulfide bonds. The role of this activated factor IX in the blood coagulation cascade is to activate factor X to its active form through interactions with Ca+2 ions, membrane phospholipids, and factor VIII. Alterations of this gene, including point mutations, insertions and deletions, cause factor IX deficiency, which is a recessive X-linked disorder, also called hemophilia B or Christmas disease. Normal concentrations of factor IX in human plasma is app. 3 ug/ml.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol F9
Entrez