Recombinant Human FGF-10 Protein


1 μg/lane of Recombinant Human FGF-10 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 21 kDa.
Recombinant Human FGF-10 (Catalog # 345-FG) stimulates cell proliferation of the 4MBr‑5 rhesus monkey epithelial cell line. The ED50 for this effect is 20‑100 ng/mL.

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human FGF-10 Protein Summary

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Details of Functionality
Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is 20-100 ng/mL.
E. coli-derived human FGF-10 protein
Cys37-Ser208 & Gly41-Ser208
Accession #
N-terminal Sequence
Cys37 & Gly41
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
19.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
19-22 kDa, reducing conditions
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345-FG in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT with BSA as a carrier protein.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FGF-10 Protein

  • FGF10
  • FGF-10
  • fibroblast growth factor 10
  • Keratinocyte growth factor 2
  • KGF2
  • KGF-2
  • produced by fibroblasts of urinary bladder lamina propria


The Fibroblast Growth Factors (FGFs) are heparin binding glycoproteins that exert a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. FGF-10 belongs to the subgroup of FGFs that also includes FGF-3, -7, and -22 (1). Mature human FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP (1, 4). It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb) (5). The mitogenic and chemotactic properties of FGF-10 are critical in many tissues during embryogenesis. This includes limb bud initiation (6), palate development (7), branching morphogenesis and directional outgrowth of lung buds (8, 9), formation of the otic vesicle and chochlea (10), adipogenesis (11), and the development of prostate, mammary, lacrimal, and submandibular salivary glands (12 - 15). FGF R2 (IIIb) signaling in these responsive tissues is similarly important during embryogenesis (7, 10, 13 ‑ 15). The expression and function of FGF-10 are negatively regulated by Shh and BMP-4 in the developing lung (8, 9). Overlapping expression patterns and activities with FGF-3, -7,  and -8 suggest at least a partial redundancy in FGF‑10 biology (7, 10, 14, 15). FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer (16).
  1. Beenken, A. and M. Mohammadi (2009) Nat. Rev. Drug Discov. 8:235.
  2. Igarashi, M. et al. (1998) J. Biol. Chem. 273:13230.
  3. Emoto, H. et al. (1997) J. Biol. Chem. 272:23191.
  4. Beer, H.-D. et al. (2005) Oncogene 24:5269.
  5. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
  6. Min, H. et al. (1998) Genes Dev. 12:3156.
  7. Rice, R. et al. (2004) J. Clin. Invest. 113:1692.
  8. Bellusci, S. et al. (1997) Development 124:4867.
  9. Weaver, M. et al. (2000) Development 127:2695.
  10. Pirvola, U. et al. (2000) J. Neurosci. 20:6125.
  11. Sakaue, H. et al. (2002) Genes Dev. 16:908.
  12. Donjacour, A.A. et al. (2003) Dev. Biol. 261:39.
  13. Mailleux, A.A. et al. (2002) Development 129:53.
  14. Makarenkova, H.P. et al. (2000) Development 127:2563.
  15. Jaskoll, T. et al. (2005) BMC Dev. Biol. 5:11.
  16. Nomura, S. et al. (2008) Br. J. Cancer 99:305.

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Publications for FGF-10 (345-FG)(78)

We have publications tested in 5 confirmed species: Human, Mouse, Canine, Xenopus, Zebrafish.

We have publications tested in 5 applications: Binding Assay, Bioassay, Cell Culture, Differentiation, In Vivo.

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Showing Publications 1 - 10 of 78. Show All 78 Publications.
Publications using 345-FG Applications Species
S Imamura, K Yoshimoto, S Terada, K Takamuro, KI Kamei In vitro culture at 39��C during hepatic maturation of human ES cells facilitates hepatocyte-like cell functions Scientific Reports, 2022;12(1):5155. 2022 [PMID: 35338220] (Bioassay, Human) Bioassay Human
AK Eicher, DO Kechele, N Sundaram, HM Berns, HM Poling, LE Haines, JG Sanchez, K Kishimoto, M Krishnamur, L Han, AM Zorn, MA Helmrath, JM Wells Functional human gastrointestinal organoids can be engineered from three primary germ layers derived separately from pluripotent stem cells Cell Stem Cell, 2021;0(0):. 2021 [PMID: 34856121] (Bioassay, Human) Bioassay Human
L Johnson Ch, H Lahlou, C Steinacher, S Assou, Y Messat, J Dudás, A Edge, B Crespo, M Crosier, C Sergi, A Schrott-Fi, A Zine Transcriptome-Wide Analysis Reveals a Role for Extracellular Matrix and Integrin Receptor Genes in Otic Neurosensory Differentiation from Human iPSCs International Journal of Molecular Sciences, 2021;22(19):. 2021 [PMID: 34639189] (Bioassay, Human) Bioassay Human
K Gkatzis, P Panza, S Peruzzo, DY Stainier Differentiation of mouse fetal lung alveolar progenitors in serum-free organotypic cultures Elife, 2021;10(0):. 2021 [PMID: 34586063] (Bioassay, Mouse) Bioassay Mouse
Y Aghazadeh, F Poon, F Sarangi, FTM Wong, ST Khan, X Sun, R Hatkar, BJ Cox, SS Nunes, MC Nostro Microvessels support engraftment and functionality of human islets and hESC-derived pancreatic progenitors in diabetes models Cell Stem Cell, 2021;0(0):. 2021 [PMID: 34480863] (Bioassay, Human) Bioassay Human
MO Lee, SG Lee, CR Jung, YS Son, JW Ryu, KB Jung, JH Ahn, JH Oh, HA Lee, JH Lim, J Kim, I Jang, J Choi, J Jung, K Park, B Lee, DS Kim, MY Son, HS Cho Development of a quantitative prediction algorithm for target organ-specific similarity of human pluripotent stem cell-derived organoids and cells Nature Communications, 2021;12(1):4492. 2021 [PMID: 34301945] (Bioassay, Human) Bioassay Human
AL Rodrigues, HY Liu, YW Chen, M Porotto, A Moscona, HW Snoeck The in vitro multilineage differentiation and maturation of lung and airway cells from human pluripotent stem cell-derived lung progenitors in 3D Nature Protocols, 2021;0(0):. 2021 [PMID: 33649566] (Bioassay, Human) Bioassay Human
H Tomita, K Tanaka, A Hirata, H Okada, H Imai, Y Shirakami, K Ohnishi, S Sugie, H Aoki, Y Hatano, K Noguchi, T Kanayama, A Niwa, N Suzui, T Miyazaki, T Tanaka, H Akiyama, M Shimizu, K Yoshida, A Hara Inhibition of FGF10-ERK signal activation suppresses intraductal papillary neoplasm of the bile duct and its associated carcinomas Cell Reports, 2021;34(8):108772. 2021 [PMID: 33626352] (Human) Human
L Cui, J Zheng, Q Zhao, JR Chen, H Liu, G Peng, Y Wu, C Chen, Q He, H Shi, S Yin, RA Friedman, Y Chen, MX Guan Mutations of MAP1B encoding a microtubule-associated phosphoprotein cause sensorineural hearing loss JCI Insight, 2020;5(23):. 2020 [PMID: 33268592] (Bioassay, Human) Bioassay Human
FJ Hawkins, S Suzuki, ML Beermann, C Barillà, R Wang, C Villacorta, A Berical, JC Jean, J Le Suer, T Matte, C Simone-Roa, Y Tang, TM Schlaeger, AM Crane, N Matthias, SXL Huang, SH Randell, J Wu, JR Spence, G Carraro, BR Stripp, A Rab, EJ Sorsher, A Horani, SL Brody, BR Davis, DN Kotton Derivation of Airway Basal Stem Cells from Human Pluripotent Stem Cells Cell Stem Cell, 2020;0(0):. 2020 [PMID: 33098807] (Bioassay, Human) Bioassay Human
Show All 78 Publications.

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Gene Symbol FGF10