Recombinant Human FGF-10 Protein


1 μg/lane of Recombinant Human FGF-10 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 21 kDa.
Recombinant Human FGF-10 (Catalog # 345-FG) stimulates cell proliferation of the 4MBr‑5 rhesus monkey epithelial cell line. The ED50 for this effect is 20‑100 ng/mL.

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human FGF-10 Protein Summary

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Details of Functionality
Measured in a cell proliferation assay using 4MBr‑5 rhesus monkey epithelial cells. Rubin, J.S. et al. (1989) Proc. Natl. Acad. Sci. USA 86:802. The ED50 for this effect is 20-100 ng/mL.
E. coli-derived human FGF-10 protein
Cys37-Ser208 & Gly41-Ser208
Accession #
N-terminal Sequence
Cys37 & Gly41
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
19.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
19-22 kDa, reducing conditions
Read Publications using
345-FG in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in MOPS, Na2SO4, EDTA and DTT with BSA as a carrier protein.
>97%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human FGF-10 Protein

  • FGF10
  • FGF-10
  • fibroblast growth factor 10
  • Keratinocyte growth factor 2
  • KGF2
  • KGF-2
  • produced by fibroblasts of urinary bladder lamina propria


The Fibroblast Growth Factors (FGFs) are heparin binding glycoproteins that exert a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. FGF-10 belongs to the subgroup of FGFs that also includes FGF-3, -7, and -22 (1). Mature human FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). It shares 93% and 96% amino acid sequence identity with mouse and rat FGF-10, respectively. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP (1, 4). It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb) (5). The mitogenic and chemotactic properties of FGF-10 are critical in many tissues during embryogenesis. This includes limb bud initiation (6), palate development (7), branching morphogenesis and directional outgrowth of lung buds (8, 9), formation of the otic vesicle and chochlea (10), adipogenesis (11), and the development of prostate, mammary, lacrimal, and submandibular salivary glands (12 - 15). FGF R2 (IIIb) signaling in these responsive tissues is similarly important during embryogenesis (7, 10, 13 ‑ 15). The expression and function of FGF-10 are negatively regulated by Shh and BMP-4 in the developing lung (8, 9). Overlapping expression patterns and activities with FGF-3, -7,  and -8 suggest at least a partial redundancy in FGF‑10 biology (7, 10, 14, 15). FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer (16).
  1. Beenken, A. and M. Mohammadi (2009) Nat. Rev. Drug Discov. 8:235.
  2. Igarashi, M. et al. (1998) J. Biol. Chem. 273:13230.
  3. Emoto, H. et al. (1997) J. Biol. Chem. 272:23191.
  4. Beer, H.-D. et al. (2005) Oncogene 24:5269.
  5. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
  6. Min, H. et al. (1998) Genes Dev. 12:3156.
  7. Rice, R. et al. (2004) J. Clin. Invest. 113:1692.
  8. Bellusci, S. et al. (1997) Development 124:4867.
  9. Weaver, M. et al. (2000) Development 127:2695.
  10. Pirvola, U. et al. (2000) J. Neurosci. 20:6125.
  11. Sakaue, H. et al. (2002) Genes Dev. 16:908.
  12. Donjacour, A.A. et al. (2003) Dev. Biol. 261:39.
  13. Mailleux, A.A. et al. (2002) Development 129:53.
  14. Makarenkova, H.P. et al. (2000) Development 127:2563.
  15. Jaskoll, T. et al. (2005) BMC Dev. Biol. 5:11.
  16. Nomura, S. et al. (2008) Br. J. Cancer 99:305.

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Publications for FGF-10 (345-FG)(63)

We have publications tested in 5 confirmed species: Human, Mouse, Canine, Xenopus, Zebrafish.

We have publications tested in 5 applications: Binding Assay, Bioassay, Cell Culture, Differentiation, In Vivo.

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Showing Publications 1 - 10 of 63. Show All 63 Publications.
Publications using 345-FG Applications Species
R Matsumoto, H Suga, T Aoi, H Bando, H Fukuoka, G Iguchi, S Narumi, T Hasegawa, K Muguruma, W Ogawa, Y Takahashi Congenital pituitary hypoplasia model demonstrates hypothalamic OTX2 regulation of pituitary progenitor cells J. Clin. Invest., 2020;130(2):641-654. 2020 [PMID: 31845906] (Bioassay, Human) Bioassay Human
A Sahabian, M Sgodda, O Naujok, R Dettmer, J Dahlmann, F Manstein, T Cantz, R Zweigerdt, U Martin, R Olmer Chemically-Defined, Xeno-Free, Scalable Production of hPSC-Derived Definitive Endoderm Aggregates with Multi-Lineage Differentiation Potential Cells, 2019;8(12):. 2019 [PMID: 31817235] (Bioassay, Human) Bioassay Human
W Liang, P Han, EH Kim, J Mak, R Zhang, AG Torrente, JI Goldhaber, E Marbán, HC Cho Canonical Wnt signaling promotes pacemaker cell specification of cardiac mesodermal cells derived from mouse and human embryonic stem cells Stem Cells, 2019;0(0):. 2019 [PMID: 31648393] (Bioassay, Mouse) Bioassay Mouse
T Yung, F Poon, M Liang, S Coquenlorg, EC McGaugh, CC Hui, MD Wilson, MC Nostro, TH Kim Sufu- and Spop-mediated downregulation of Hedgehog signaling promotes beta cell differentiation through organ-specific niche signals Nat Commun, 2019;10(1):4647. 2019 [PMID: 31604927] (Bioassay, Human) Bioassay Human
V Lungova, X Chen, Z Wang, C Kendziorsk, SL Thibeault Human induced pluripotent stem cell-derived vocal fold mucosa mimics development and responses to smoke exposure Nat Commun, 2019;10(1):4161. 2019 [PMID: 31551422] (Bioassay, Human) Bioassay Human
K Suzuki, M Koyanagi-A, K Uehara, N Hinata, M Fujisawa, T Aoi Directed differentiation of human induced pluripotent stem cells into mature stratified bladder urothelium Sci Rep, 2019;9(1):10506. 2019 [PMID: 31324820] (Cell Culture, Human) Cell Culture Human
RMJ Genga, EM Kernfeld, KM Parsi, TJ Parsons, MJ Ziller, R Maehr Single-Cell RNA-Sequencing-Based CRISPRi Screening Resolves Molecular Drivers of Early Human Endoderm Development Cell Rep, 2019;27(3):708-718.e10. 2019 [PMID: 30995470] (Bioassay, Human) Bioassay Human
Y Toba, A Kiso, S Nakamae, F Sakurai, K Takayama, H Mizuguchi FGF signal is not required for hepatoblast differentiation of human iPS cells Sci Rep, 2019;9(1):3713. 2019 [PMID: 30842525] (Bioassay, Human) Bioassay Human
H Noguchi, C Miyagi-Shi, Y Nakashima, T Kinjo, N Kobayashi, I Saitoh, M Watanabe, AMJ Shapiro, T Kin Induction of Expandable Tissue-Specific Progenitor Cells from Human Pancreatic Tissue through Transient Expression of Defined Factors Mol Ther Methods Clin Dev, 2019;13(0):243-252. 2019 [PMID: 30828587] (Bioassay, Human) Bioassay Human
C Kumtornrut, T Yamauchi, S Koike, S Aiba, K Yamasaki Androgens modulate keratinocyte differentiation indirectly through enhancing growth factor production from dermal fibroblasts J. Dermatol. Sci., 2019;0(0):. 2019 [PMID: 30792099] (Bioassay, Human) Bioassay Human
Show All 63 Publications.

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Gene Symbol FGF10