FGF-21 Antibody

Western Blot: FGF-21 Antibody [NBP1-31368] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with FGF21 antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: FGF-21 Antibody [NBP1-31368] - Analysis of HeLa, using NBP1-31368 at 1:200 dilution.

Immunohistochemistry-Paraffin: FGF-21 Antibody [NBP1-31368] - Paraffin-embedded HS578T xenograft, using antibody at 1:500 dilution.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

FGF-21 Antibody Summary

• Description: Novus Biologicals Rabbit FGF-21 Antibody (NBP1-31368) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FGF-21. The exact sequence is proprietary.
• Localization: Secreted
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FGF21
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 22 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 1% BSA, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for FGF-21 Antibody

• FGF21
• FGF-21
• fibroblast growth factor 21

Background

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for FGF-21 Antibody (NBP1-31368) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 03/28/2014

Summary

• Application: Western Blot
• Sample Tested: Primary muscle cell lysate
• Species: Human

Bioinformatics

• Gene Symbol: FGF21