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FGF-21 Antibody

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Western Blot: FGF-21 Antibody [NBP1-31368] - Sample (30 ug of whole cell lysate) A: Hep G2 B: Raji 12% SDS PAGE; antibody diluted at 1:1000.
Western Blot: FGF-21 Antibody [NBP1-31368] - Sample (30 ug of whole cell lysate) A: Hep G2 B: Raji 12% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: FGF-21 Antibody [NBP1-31368] - Analysis of HeLa, using NBP1-31368 at 1:200 dilution.
Immunohistochemistry-Paraffin: FGF-21 Antibody [NBP1-31368] - Paraffin-embedded HS578T xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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FGF-21 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FGF21. The exact sequence is proprietary.
Localization
Secreted
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FGF21
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-31368 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol (pH7)
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for FGF-21 Antibody

  • FGF21
  • FGF-21
  • fibroblast growth factor 21

Background

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - Analysis of 25 ug of Hela (Lane 1), Jurkat (Lane 2), and NIH-3T3 cell lysates (Lane 3) and a molecular weight protein ladder.
Flow Cytometry: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - An intracellular stain was performed on Jurkat cells with PPAR alpha/NR1C1 Antibody (3B6/PPAR) NB300-537AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.
Western Blot: PPAR alpha/NR1C1 Antibody (3B6/PPAR) [NB300-537] - 293T cell lysate. Image from verified customer review.
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Western Blot: PPAR gamma/NR1C3 Antibody [NBP2-22106] - Total protein from 3T3-L1 mouse embryonic fibroblast adipose-like cell line, separated by 4-12% SDS-PAGE, transferred to nitrocellulose membrane and blocked in 5% non-fat milk for 1h at room temperature. The membrane was probed with anti-PPAR gamma 1:800 in non-fat milk. Lane 2-7 increasing protein concentration, undifferentiated adipocytes. Lane 8-13 increasing protein concentration, differentiated adipocytes. This image was submitted via customer review.
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Western Blot: PPAR gamma Antibody [NBP2-22106] - Western blot analysis of PPAR gamma in 1. human adipose and 2. human adrenal lysate.
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Western Blot: FGF R1 Antibody (M19B2) [NB600-1287] - Western blot analysis of MCF7 (A), HepG2 (B), NIH3T3 (C), and PC-12 (D) cell lysate using FGF R1 antibody at 2 ug/ml.
Flow Cytometry: FGF R1 Antibody (M19B2) [NB600-1287] - Analysis using the Alexa Fluor (R) 488 conjugate of NB600-1287. Staining of FGF R1 in HEK293 and HEK293 FGFR transfected cells using anti-FGF R1 antibody. Red - positive cells, Green - negative cells, Blue - negative cells + antibody, Pink - positive cells + antibody. Image from verified customer review.
Flow Cytometry: FGF R1 Antibody (M19B2) [NB600-1287] - Intracellular flow cytometric staining of 1 x 10^6 HEK-293 cells using FGF R1 antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.
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Western Blot: FGF R2 Antibody (5C5) [NBP2-00932] - Analysis of FGF R2 in HEK293T cells (left, control) and HEK293T cells overexpressing FGF R2 (right) using anti-FGF R2 antibody. Image from verified customer review.
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1 μg/lane of Recombinant Human IGF-I was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.
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Western blot shows lysates of mouse heart tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse FGF R4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2265) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FGF R4 at approximately 89 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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FGF-21 Antibody
NBP1-31368
FGF-21 Antibody
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P

Publications for FGF-21 Antibody (NBP1-31368) (0)

There are no publications for FGF-21 Antibody (NBP1-31368).
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Review for FGF-21 Antibody (NBP1-31368) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-31368:
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Images Ratings Applications Species Date Details
Western Blot FGF-21 NBP1-31368
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reviewed by:
Anonymous
 WB Human 03/28/2014
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Summary

ApplicationWestern Blot
Sample TestedPrimary muscle cell lysate
SpeciesHuman
CommentsAntibody worked well; it detecs a clear band at 20 kDa as predicted as well as bands at 50 and 15 kDa respectively.

Blocking

Blocking Details5% BSA for 1 hour at RT

Primary Anitbody

Dilution Ratio1:1000 in 5% BSA, incubated overnight at 4C

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP,
Secondary Manufacturer Cat#DAKO P044801-2
Secondary Concentration1: 5000 in 5% BSA

Comments

CommentsAntibody worked well; it detecs a clear band at 20 kDa as predicted as well as bands at 50 and 15 kDa respectively.

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FGF-21 Antibody (NBP1-31368) (0)

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Secondary Antibodies

 

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Western Blot FGF-21 NBP1-31368
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 Anonymous
03/28/2014
Application: WB
Species: Human
read this review

Bioinformatics

Gene Symbol FGF21
Entrez