FGF-21 Antibody

Images

 
Western Blot: FGF-21 Antibody [NBP1-31368] - Sample (30 ug of whole cell lysate) A: Hep G2 B: Raji 12% SDS PAGE; antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: FGF-21 Antibody [NBP1-31368] - Analysis of HeLa, using NBP1-31368 at 1:200 dilution.
Immunohistochemistry-Paraffin: FGF-21 Antibody [NBP1-31368] - Paraffin-embedded HS578T xenograft, using antibody at 1:500 dilution.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

FGF-21 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human FGF21. The exact sequence is proprietary.
Localization
Secreted
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
FGF21
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-31368 in the following applications:

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
0.1M Tris (pH 7.0), 0.1M Glycine and 10% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for FGF-21 Antibody

  • FGF21
  • FGF-21
  • fibroblast growth factor 21

Background

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. The function of this growth factor has not yet been determined.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for FGF-21 Antibody (NBP1-31368) (0)

There are no publications for FGF-21 Antibody (NBP1-31368).
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Review for FGF-21 Antibody (NBP1-31368) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: WB.

Reviews using NBP1-31368:
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WB
(1)
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(1)
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Images Ratings Applications Species Date Details
Western Blot FGF-21 NBP1-31368
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reviewed by:
Anonymous
WB Human 03/28/2014
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Summary

ApplicationWestern Blot
Sample TestedPrimary muscle cell lysate
SpeciesHuman
CommentsAntibody worked well; it detecs a clear band at 20 kDa as predicted as well as bands at 50 and 15 kDa respectively.

Blocking

Blocking Details5% BSA for 1 hour at RT

Primary Anitbody

Dilution Ratio1:1000 in 5% BSA, incubated overnight at 4C

Secondary Antibody

Secondary DescriptionGoat anti-rabbit IgG, HRP,
Secondary Manufacturer Cat#DAKO P044801-2
Secondary Concentration1: 5000 in 5% BSA

Comments

CommentsAntibody worked well; it detecs a clear band at 20 kDa as predicted as well as bands at 50 and 15 kDa respectively.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for FGF-21 Antibody (NBP1-31368) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for FGF-21 Antibody (NBP1-31368)

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Blogs on FGF-21

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Calculators

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Recent Reviews

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5
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4
1
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2
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1
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Anonymous
03/28/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol FGF21
Entrez