FGF-21 Antibodies

HAF019). A specific band was detected for FGF-21 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF019). A specific band was detected for FGF-21 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of mouse spleen tissue and rat spleen tissue. PVDF membrane was probed with 0.25 µg/mL of Goat Anti-Mouse FGF-21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3057) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for FGF-21 at approximately 26 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

MAB25374 coated on a Clear Polystyrene Microplate DY990. Goat Anti-Human FGF‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2539) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP DY998 followed by Substrate Solution DY999 and stopping the enzymatic reaction with Stop Solution DY994." class="big_lightbox"> MAB25374 coated on a Clear Polystyrene Microplate DY990. Goat Anti-Human FGF‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2539) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP DY998 followed by Substrate Solution DY999 and stopping the enzymatic reaction with Stop Solution DY994." alt="Recombinant Human FGF‑21 protein was serially diluted 2-fold and captured by Mouse Anti-Human FGF‑21 Monoclonal Antibody MAB25374 coated on a Clear Polystyrene Microplate DY990. Goat Anti-Human FGF‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2539) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP DY998 followed by Substrate Solution DY999 and stopping the enzymatic reaction with Stop Solution DY994." class="big_thumb" />

2539-FG) was serially diluted and captured by Mouse Anti-Human FGF‑21 Monoclonal Antibody (Catalog # MAB25374) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human FGF‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2539) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 2539-FG) was serially diluted and captured by Mouse Anti-Human FGF‑21 Monoclonal Antibody (Catalog # MAB25374) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human FGF‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2539) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_lightbox"> MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." alt="HepG2 human hepatocellular carcinoma cell line was stained with Mouse Anti-Human FGF-21 Monoclonal Antibody (Catalog # MAB25372, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox"> NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />

DY990). Goat Anti-Human FGF-21 Antigen Affinity-purified Polyclonal Antibody (AF2539) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_lightbox"> DY990). Goat Anti-Human FGF-21 Antigen Affinity-purified Polyclonal Antibody (AF2539) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." alt="Recombinant Human FGF‑21 protein was serially diluted 2-fold and captured by Mouse Anti-Human FGF-21 Monoclonal Antibody (Catalog # MAB25374) coated on a Clear Polystyrene Microplate (DY990). Goat Anti-Human FGF-21 Antigen Affinity-purified Polyclonal Antibody (AF2539) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (DY998) followed by Substrate Solution (DY999) and stopping the enzymatic reaction with Stop Solution (DY994)." class="big_thumb" />

2539-FG) was serially diluted and captured by Mouse Anti-Human FGF‑21 Monoclonal Antibody (Catalog # MAB25374) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human FGF‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2539) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 2539-FG) was serially diluted and captured by Mouse Anti-Human FGF‑21 Monoclonal Antibody (Catalog # MAB25374) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human FGF‑21 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2539) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

2619-KB), Recombinant Human FGF-21 (Catalog # 2539-FG) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with human FGF-RIIIc in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human FGF-21 (500 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-21 Mononclonal Antibody (MAB25373). The ND₅₀ is typically 0.5-3 µg/mL." class="big_lightbox"> 2619-KB), Recombinant Human FGF-21 (Catalog # 2539-FG) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with human FGF-RIIIc in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human FGF-21 (500 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-21 Mononclonal Antibody (MAB25373). The ND₅₀ is typically 0.5-3 µg/mL." alt="In the presence of Recombinant Mouse Klotho beta (Catalog # 2619-KB), Recombinant Human FGF-21 (Catalog # 2539-FG) stimulates proliferation in the BaF3 mouse pro-B cell line transfected with human FGF-RIIIc in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human FGF-21 (500 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human FGF-21 Mononclonal Antibody (MAB25373). The ND₅₀ is typically 0.5-3 µg/mL." class="big_thumb" />