Klotho beta Antibody

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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Klotho beta Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Klotho beta (R&D Systems, Catalog # 2619-KB)
Phe53-Leu995
Accession # NP_112457
Specificity
Detects mouse Klotho beta  in direct ELISAs and Western blots. In these formats, approximately 5% cross‑reactivity with recombinant mouse Klotho is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
KLB
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
Publications
Read Publications using
AF2619 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Klotho beta Antibody

  • betaKlotho
  • beta-klotho
  • BKL
  • KLB
  • klotho beta like
  • Klotho beta
  • klotho beta-like protein
  • MGC142213

Background

Klotho beta , a divergent structural member of the glycosidase I superfamily, is expressed primarily in the liver and pancreas, with lower expression in adipose tissue (1). Like Klotho, Klotho beta facilitates binding between FGF19 subfamily members and their receptors via formation of a ternary complex (2). The Klotho beta mediated interaction of FGF15 (human FGF19) with FGF Receptor 4 in the liver negatively regulates bile acid synthesis by controlling the secretion of two key bile acid synthase genes, cholesterol 7-alpha hydroxylase (Cyp7a1) and sterol 12-alpha hydroxylase (Cyp8b1) (2 - 4). Klotho beta is also a cofactor for the interaction of FGF21 with FGF Receptor 1c in adipocytes, which allows FGF21 to stimulate GLUT1 expression, upregulating adipocyte insulin-dependent glucose uptake (2 - 3, 5). The 1043 amino acid (aa) type I transmembrane protein is composed of a 51 aa signal sequence, a 943 aa extracellular domain (ECD) containing two glycosidase-like regions, a 21 aa transmembrane domain, and 28 aa intracellular tail. Since Klotho-related proteins lack critical active site Glu residues present in beta -glycosidases, it was initially unclear whether they were functional enzymes (1, 6). However, glucuronidase activity has since been demonstrated for Klotho, indicating that physiologically relevant enzymatic activity for Klotho beta is also possible (7). The extracellular domain shares 79%, 79%, 80% and 67% identity with human, bovine, canine and rat Klotho beta , respectively. The low identity with rat reflects aa discordance within rodent ECD.

  1. Mian, I.S. (1998) Blood Cells Mol. Dis. 24:83.
  2. Ito, S. et al. (2005) J. Clin. Invest. 115:2202.
  3. Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687.
  4. Lin, B. C. et al. (2007) J. Biol. Chem. 282:27277.
  5. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci USA 104:7432.
  6. Chang, Q. et al. (2005) Science 310:490.
  7. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Klotho beta Antibody (AF2619)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: IHC, Western Blot.


Filter By Application
IHC
(1)
Western Blot
(10)
All Applications
Filter By Species
Human
(1)
Mouse
(9)
All Species
Showing Publications 1 - 10 of 10.
Publications using AF2619 Applications Species
YK Wu, ZN Ren, SL Zhu, YZ Wu, G Wang, H Zhang, W Chen, Z He, XL Ye, QX Zhai Sulforaphane ameliorates non-alcoholic fatty liver disease in mice by promoting FGF21/FGFR1 signaling pathway Acta pharmacologica Sinica, 2021-10-15;0(0):. 2021-10-15 [PMID: 34654875] (Western Blot, Mouse) Western Blot Mouse
CA Gilroy, S Roberts, A Chilkoti Fusion of fibroblast growth factor 21 to a thermally responsive biopolymer forms an injectable depot with sustained anti-diabetic action J Control Release, 2018-03-15;0(0):. 2018-03-15 [PMID: 29551712] (Western Blot, Mouse) Western Blot Mouse
LD BonDurant, M Ameka, MC Naber, KR Markan, SO Idiga, MR Acevedo, SA Walsh, DM Ornitz, MJ Potthoff FGF21 Regulates Metabolism Through Adipose-Dependent and -Independent Mechanisms Cell Metab., 2017-04-04;25(4):935-944.e4. 2017-04-04 [PMID: 28380381] (Western Blot, Human) Western Blot Human
Keipert S, Ost M, Johann K, Imber F, Jastroch M, van Schothorst E, Keijer J, Klaus S Skeletal muscle mitochondrial uncoupling drives endocrine cross-talk through the induction of FGF21 as a myokine. Am J Physiol Endocrinol Metab, 2013-12-17;306(5):E469-82. 2013-12-17 [PMID: 24347058] (Western Blot, Mouse) Western Blot Mouse
Fu T, Choi S, Kim D, Seok S, Suino-Powell K, Xu H, Kemper J Aberrantly elevated microRNA-34a in obesity attenuates hepatic responses to FGF19 by targeting a membrane coreceptor beta-Klotho. Proc Natl Acad Sci U S A, 2012-09-17;109(40):16137-42. 2012-09-17 [PMID: 22988100] (IHC, Western Blot, Mouse) IHC, Western Blot Mouse
Veniant MM, Hale C, Helmering J, Chen MM, Stanislaus S, Busby J, Vonderfecht S, Xu J, Lloyd DJ FGF21 Promotes Metabolic Homeostasis via White Adipose and Leptin in Mice. PLoS ONE, 2012-07-06;7(7):e40164. 2012-07-06 [PMID: 22792234] (Western Blot, Mouse) Western Blot Mouse
Wu S, Levenson A, Kharitonenkov A, De Luca F Fibroblast Growth Factor 21 (FGF21) Inhibits Chondrocyte Function and Growth Hormone Action Directly at the Growth Plate. J. Biol. Chem., 2012-06-13;287(31):26060-7. 2012-06-13 [PMID: 22696219] (Western Blot, Mouse) Western Blot Mouse
Gomez IG, Tang J, Wilson CL, Yan W, Heinecke JW, Harlan JM, Raines EW Metalloproteinase-mediated Shedding of Integrin beta2 promotes macrophage efflux from inflammatory sites. J. Biol. Chem., 2011-12-14;287(7):4581-9. 2011-12-14 [PMID: 22170060] (Western Blot, Mouse) Western Blot Mouse
Micanovic R, Raches DW, Dunbar JD, Driver DA, Bina HA, Dickinson CD, Kharitonenkov A Different roles of N- and C- termini in the functional activity of FGF21. J. Cell. Physiol., 2009-05-01;219(2):227-34. 2009-05-01 [PMID: 19117008] (Western Blot, Mouse) Western Blot Mouse
Ogawa Y, Kurosu H, Yamamoto M, Nandi A, Rosenblatt KP, Goetz R, Eliseenkova AV, Mohammadi M, Kuro-o M BetaKlotho is required for metabolic activity of fibroblast growth factor 21. Proc. Natl. Acad. Sci. U.S.A., 2007-04-23;104(18):7432-7. 2007-04-23 [PMID: 17452648] (Western Blot, Mouse) Western Blot Mouse

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Bioinformatics

Gene Symbol KLB
Uniprot