Ephrin-B2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse embryo tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Mouse/Rat Ephrin‑B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF496) followed by ...read more
Ephrin‑B2 was detected in immersion fixed rat hippocampal neurons using 2 µg/mL Goat Anti-Human/Mouse/Rat Ephrin‑B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF496) for 3 hours at room temperature. ...read more
SH‑SY5Y human neuroblastoma cell line was stained with Goat Anti-Human/Mouse/Rat Ephrin‑B2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF496, filled histogram) or isotype control antibody (Catalog # ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Ephrin-B2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Ephrin-B2
Arg27-Ala227
Accession # AAA82934
Specificity
Detects mouse Ephrin-B2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 5% cross-reactivity with recombinant mouse Ephrin-B1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EFNB2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF496 in the following applications:

Publications
Read Publications using
AF496 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Ephrin-B2 Antibody [Unconjugated]

  • EFNB2
  • ELF-2
  • ephrin B2
  • EphrinB2
  • Ephrin-B2
  • EPLG5
  • HTKL
  • Htk-L
  • LERK5
  • LERK-5
  • NLERK-1

Background

Ephrin-B2, also known as Htk-L, ELF-2, LERK-5, and NLERK-1 (1), is a member of the ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids and includes four invariant cysteines. The B-class ligands are transmembrane proteins which can become tyrosine phosphorylated upon receptor ligation. The cytoplasmic domains are approximately 80 amino acids long and are highly conserved, especially the last 33 amino acids. Several signaling molecules have been shown to interact with the cytoplasmic region, although specific signaling roles have yet to be elucidated. Ephrin-B2 has been shown to bind EphA4, EphB1, EphB2, EphB3, and EphB4 (2, 3). The extracellular domains of murine and human Ephrin-B2 share 98% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The Ephrin/Eph families also appear to play a role in angiogenesis (3).

  1. Eph Nomenclature Committee [letter]. (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Ephrin-B2 Antibody (AF496)(26)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 9 applications: Cell Culture Treatment, Flow Cytometry, ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Proximity Ligation Assay (PLA), Western Blot.


Filter By Application
Cell Culture Treatment
(1)
Flow Cytometry
(2)
ICC
(2)
IHC
(7)
IHC-Fr
(1)
IHC-P
(1)
Immunoprecipitation
(1)
Proximity Ligation Assay (PLA)
(1)
Western Blot
(10)
All Applications
Filter By Species
Human
(5)
Mouse
(18)
Rat
(3)
All Species
Showing Publications 1 - 10 of 26. Show All 26 Publications.
Publications using AF496 Applications Species
K Takaya, K Okabe, A Ishigami, Y Imbe, H Kanazawa, S Sakai, N Aramaki-Ha, K Kishi Actin cable formation and epidermis-dermis positional relationship during complete skin regeneration Scientific Reports, 2022;12(1):15913. 2022 [PMID: 36151111] (IHC, Mouse) IHC Mouse
H Maruoka, S Zhao, H Yoshino, M Abe, T Yamamoto, H Hongo, M Haraguchi-, A Nasoori, H Ishizu, Y Nakajima, M Omaki, T Shimizu, N Iwasaki, PH Luiz de Fr, M Li, T Hasegawa Histochemical examination of blood vessels in murine femora with intermittent PTH administration Journal of oral biosciences, 2022;0(0):. 2022 [PMID: 35584768] (IHC, Mouse) IHC Mouse
S Yamasaki, A Kuwahara, A Kishino, T Kimura, M Takahashi, M Mandai Addition of Chk1 inhibitor and BMP4 cooperatively promotes retinal tissue formation in self-organizing human pluripotent stem cell differentiation culture Regenerative Therapy, 2022;19(0):24-34. 2022 [PMID: 35059477] (IHC, Human) IHC Human
Y Wang, C Shao, L Qi, J Tan, Y Zhao, M Xue, X Li, W Cheng, X Li, J Yin, Y Shi, Y Wang, K Wang, H Hu, S Yan EphrinB2-RhoA upregulation attenuates sympathetic hyperinnervation and decreases the incidence of ventricular arrhythmia after myocardial infarction Journal of cardiology, 2021;0(0):. 2021 [PMID: 34750029] (Western Blot, Mouse) Western Blot Mouse
YW Ge, K Feng, XL Liu, HF Chen, ZY Sun, CF Wang, ZQ Liu, HW Wang, JW Zhang, DG Yu, YQ Mao The Recombinant Protein EphB4-Fc Changes the Ti Particle-Mediated Imbalance of OPG/RANKL via EphrinB2/EphB4 Signaling Pathway and Inhibits the Release of Proinflammatory Factors In Vivo Oxid Med Cell Longev, 2020;2020(0):1404915. 2020 [PMID: 32587656] (ICC, Mouse) ICC Mouse
M Li, SY Nishio, C Naruse, M Riddell, S Sapski, T Katsuno, T Hikita, F Mizapoursh, FM Smith, LT Cooper, MG Lee, M Asano, T Boettger, M Krueger, A Wietelmann, J Graumann, BW Day, AW Boyd, S Offermanns, SI Kitajiri, SI Usami, M Nakayama Digenic inheritance of mutations in EPHA2 and SLC26A4 in Pendred syndrome Nat Commun, 2020;11(1):1343. 2020 [PMID: 32165640] (ICC, Mouse) ICC Mouse
E Harde, L Nicholson, B Furones Cu, D Bissen, S Wigge, S Urban, M Segarra, C Ruiz de Al, A Acker-Palm EphrinB2 regulates VEGFR2 during dendritogenesis and hippocampal circuitry development Elife, 2019;8(0):. 2019 [PMID: 31868584] (Western Blot, Mouse) Western Blot Mouse
W Shi, Y Wang, J Peng, S Qi, N Vitale, N Kaneda, T Murata, H Luo, J Wu EPHB6 controls catecholamine biosynthesis by up-regulating tyrosine hydroxylase transcription in adrenal gland chromaffin cells J. Biol. Chem., 2019;0(0):. 2019 [PMID: 30824540] (Cell Culture Treatment, Mouse) Cell Culture Treatment Mouse
M Coucha, AC Barrett, M Elgebaly, A Ergul, M Abdelsaid Inhibition of Ephrin-B2 in brain pericytes decreases cerebral pathological neovascularization in diabetic rats PLoS ONE, 2019;14(1):e0210523. 2019 [PMID: 30620753] (IHC, Rat) IHC Rat
E Groppa, S Brkic, A Uccelli, G Wirth, P Korpisalo-, M Filippova, B Dasen, V Sacchi, MG Muraro, M Trani, S Reginato, R Gianni-Bar, S Ylä-Herttu, A Banfi EphrinB2/EphB4 signaling regulates non-sprouting angiogenesis by VEGF EMBO Rep., 2018;0(0):. 2018 [PMID: 29643120] (Flow Cytometry, Human) Flow Cytometry Human
Show All 26 Publications.

Review for Ephrin-B2 Antibody (AF496) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using AF496:
Filter by Applications
Simple Western
(1)
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Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western Ephrin-B2 AF496
Enlarge
4
reviewed by:
Thomas Korff
Simple Western Mouse 07/12/2017
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Summary

ApplicationSimple Western
Sample Testedmouse brain lysate
SpeciesMouse

Comments

CommentsSimple Western: anti-ephrinB2 antibody [biotechne AF496]
Simple Western lane view shows 53 and 58kDa signals for ephrinB2 in 0.5 mg/ml of mouse brain lysate (lysis buffer from ARY014 proteome profiler kit). This run was performed under reducing conditions using the 12-230 kDa separation system in combination with a secondary anti-goat-HRP antibody (biotechne, HAF109, 1:50). Note that higher concentrations of this antibody may lead to an unspecific signal corresponding to the size of the internal standard (230 kDa, leftmost lane).

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Recent Reviews

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5
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4
1
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1
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Thomas Korff
07/12/2017
Application: Simple Western
Species: Mouse

Bioinformatics

Gene Symbol EFNB2
Uniprot