EphA4 Antibody


EphA4 was detected in immersion fixed frozen sections of embryonic rat rib cartilage primordium (E15) using 5 µg/mL Goat Anti-Mouse EphA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF641) ...read more

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Reactivity MuSpecies Glossary
Applications WB, IHC

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EphA4 Antibody Summary

Mouse myeloma cell line NS0-derived recombinant mouse EphA4
Accession # Q03137
Detects mouse EphA4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human EphA4 is observed, and less than 5% cross-reactivity with recombinant mouse (rm) EphA3, rmEphA6, rmEphA7, and rmEphA8 is observed.
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL
Read Publications using
AF641 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EphA4 Antibody

  • Cek8
  • EC 2.7.10
  • EC
  • EK8
  • EPH receptor A4
  • EphA4
  • EPH-like kinase 8
  • ephrin type-A receptor 4
  • Hek8
  • receptor protein-tyrosine kinase HEK8
  • SEK
  • Sek1
  • TYRO1 protein tyrosine kinase
  • Tyro1
  • Tyrosine-protein kinase receptor SEK
  • Tyrosine-protein kinase TYRO1


EphA4, also known as Sek, Sek1, Cek8, Hek8, and Tyro1 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA4 has been shown to bind ephrin-A5, ephrin-A1, ephrin‑A3, ephrin-A2, ephrin-B2, ephrin-B3, and ephrin-A4 (2, 3). The extracellular domains of mouse and human EphA4 share greater than 95% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

  1. Eph Nomenclature Committee [letter] (1997) Cell 9:403.
  2. Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EphA4 Antibody (AF641)(18)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 6 applications: IF, IHC, IHC-Fr, IHC/IF, Immunoprecipitation, Western Blot.

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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using AF641 Applications Species
Altounian, M;Bellon, A;Mann, F; Neuronal miR-17-5p contributes to interhemispheric cortical connectivity defects induced by prenatal alcohol exposure Cell reports 2023-08-22 [PMID: 37610874] (IHC/IF, Mouse) IHC/IF Mouse
AE Lewis, A Kuwahara, J Franzosi, JO Bush Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sorting Cell Reports, 2022-03-15;38(11):110510. 2022-03-15 [PMID: 35294885] (IF, Mouse) IF Mouse
Khoa Tran-Anh, Jingyun Zhang, Viet Tuan Nguyen-Minh, Hirofumi Fujita, Tatsumi Hirata, Izumi Sugihara Common Origin of the Cerebellar Dual Somatotopic Areas Revealed by Tracking Embryonic Purkinje Cell Clusters with Birthdate Tagging eNeuro 10/14/2020 [PMID: 33055198]
JM Hoek, WM Hepkema, W Halffman The effect of journal guidelines on the reporting of antibody validation PeerJ, 2020-06-03;8(0):e9300. 6/3/2020 [PMID: 32547887]
Beatriz Garcia-Diaz, Corinne Bachelin, Fanny Coulpier, Gaspard Gerschenfeld, Cyrille Deboux, Violetta Zujovic et al. Blood vessels guide Schwann cell migration in the adult demyelinated CNS through Eph/ephrin signaling Acta Neuropathologica 9/1/2019 [PMID: 31011859]
Zeqin Zhang, Johanne Tremblay, John Raelson, Tamar Sofer, Lizhong Du, Qiang Fang et al. EPHA4 regulates vascular smooth muscle cell contractility and is a sex-specific hypertension risk gene in individuals with type 2 diabetes Journal of Hypertension 4/1/2019 [PMID: 30817459]
Sean M. Gay, Cooper A. Brett, Jeremiah P.C. Stinson, Mark L. Gabriele Alignment of EphA4 and ephrin-B2 expression patterns with developing modularity in the lateral cortex of the inferior colliculus. Journal of Comparative Neurology 11/1/2018 [PMID: 30156295]
JM Collinson, NO Lindström, C Neves, K Wallace, C Meharg, RH Charles, ZK Ross, AM Fraser, I Mbogo, K Oras, M Nakamoto, S Barker, S Duce, Z Miedzybrod, N Vargesson The developmental and genetic basis of 'clubfoot' in the peroneal muscular atrophy mutant mouse Development, 2018-02-08;145(3):. 2018-02-08 [PMID: 29439133] (IHC, Mouse) IHC Mouse
KL Todd, KL Baker, MB Eastman, FW Kolling, AG Trausch, CE Nelson, JC Conover EphA4 regulates neuroblast and astrocyte organization in a neurogenic niche J. Neurosci, 2017-03-03;0(0):. 2017-03-03 [PMID: 28258169] (IHC, Mouse) IHC Mouse
Kao T, Nicholl G, Johansen J, Kania A, Beg A alpha2-chimaerin is required for Eph receptor-class-specific spinal motor axon guidance and coordinate activation of antagonistic muscles. J Neurosci, 2015-02-11;35(6):2344-57. 2015-02-11 [PMID: 25673830] (Western Blot, Mouse) Western Blot Mouse
Show All 18 Publications.

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Gene Symbol EPHA4