EphA4 Antibody

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EphA4 was detected in immersion fixed frozen sections of embryonic rat rib cartilage primordium (E15) using 5 µg/mL Goat Anti-Mouse EphA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF641) ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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EphA4 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EphA4
Val20-Thr547
Accession # Q03137
Specificity
Detects mouse EphA4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human EphA4 is observed, and less than 5% cross-reactivity with recombinant mouse (rm) EphA3, rmEphA6, rmEphA7, and rmEphA8 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EPHA4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
Control
EphA4 Knockout HeLa Cell Lysate
Publications
Read Publications using
AF641 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EphA4 Antibody

  • Cek8
  • EC 2.7.10
  • EC 2.7.10.1
  • EK8
  • EPH receptor A4
  • EphA4
  • EPH-like kinase 8
  • ephrin type-A receptor 4
  • Hek8
  • receptor protein-tyrosine kinase HEK8
  • SEK
  • Sek1
  • TYRO1 protein tyrosine kinase
  • Tyro1
  • Tyrosine-protein kinase receptor SEK
  • Tyrosine-protein kinase TYRO1

Background

EphA4, also known as Sek, Sek1, Cek8, Hek8, and Tyro1 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA4 has been shown to bind ephrin-A5, ephrin-A1, ephrin‑A3, ephrin-A2, ephrin-B2, ephrin-B3, and ephrin-A4 (2, 3). The extracellular domains of mouse and human EphA4 share greater than 95% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

  1. Eph Nomenclature Committee [letter] (1997) Cell 9:403.
  2. Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EphA4 Antibody (AF641)(7)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 4 applications: IHC, IHC-Fr, Immunoprecipitation, Western Blot.


Filter By Application
IHC
(3)
IHC-Fr
(2)
Immunoprecipitation
(1)
Western Blot
(1)
All Applications
Filter By Species
Mouse
(7)
All Species
Showing Publications 1 - 7 of 7.
Publications using AF641 Applications Species
JM Collinson, NO Lindström, C Neves, K Wallace, C Meharg, RH Charles, ZK Ross, AM Fraser, I Mbogo, K Oras, M Nakamoto, S Barker, S Duce, Z Miedzybrod, N Vargesson The developmental and genetic basis of 'clubfoot' in the peroneal muscular atrophy mutant mouse Development, 2018;145(3):. 2018 [PMID: 29439133] (IHC, Mouse) IHC Mouse
KL Todd, KL Baker, MB Eastman, FW Kolling, AG Trausch, CE Nelson, JC Conover EphA4 regulates neuroblast and astrocyte organization in a neurogenic niche J. Neurosci, 2017;0(0):. 2017 [PMID: 28258169] (IHC, Mouse) IHC Mouse
Kao T, Nicholl G, Johansen J, Kania A, Beg A alpha2-chimaerin is required for Eph receptor-class-specific spinal motor axon guidance and coordinate activation of antagonistic muscles. J Neurosci, 2015;35(6):2344-57. 2015 [PMID: 25673830] (Western Blot, Mouse) Western Blot Mouse
Raft S, Andrade L, Shao D, Akiyama H, Henkemeyer M, Wu D Ephrin-B2 governs morphogenesis of endolymphatic sac and duct epithelia in the mouse inner ear. Dev Biol, 2014;390(1):51-67. 2014 [PMID: 24583262] (IHC, Mouse) IHC Mouse
Kawano H, Katayama Y, Minagawa K, Shimoyama M, Henkemeyer M, Matsui T A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition. Eur. J. Immunol., 2012;42(6):1562-72. 2012 [PMID: 22622783] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Kuwako K, Kakumoto K, Imai T Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through posttranscriptional regulation of Robo3/Rig-1 expression. Neuron, 2010;67(3):407-21. 2010 [PMID: 20696379] (IHC-Fr, Mouse) IHC-Fr Mouse
Murai KK, Nguyen LN, Irie F, Yamaguchi Y, Pasquale EB Control of hippocampal dendritic spine morphology through ephrin-A3/EphA4 signaling. Nat. Neurosci., 2003;6(2):153-60. 2003 [PMID: 12496762] (IHC-Fr, Mouse) IHC-Fr Mouse

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF641

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Bioinformatics

Gene Symbol EPHA4
Uniprot