EphA4 Antibody

EphA4 was detected in immersion fixed frozen sections of embryonic rat rib cartilage primordium (E15) using 5 µg/mL Goat Anti-Mouse EphA4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF641) overnight at 4 °C. Tissue was stained (red). View our protocol for Fluorescent IHC Staining of Frozen Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

EphA4 Antibody Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse EphA4
  Val20-Thr547
  Accession # Q03137
• Specificity: Detects mouse EphA4 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human EphA4 is observed, and less than 5% cross-reactivity with recombinant mouse (rm) EphA3, rmEphA6, rmEphA7, and rmEphA8 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: EPHA4
• Purity: Immunogen affinity purified
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EphA4 Antibody

• Cek8
• EC 2.7.10
• EC 2.7.10.1
• EK8
• EPH receptor A4
• EphA4
• EPH-like kinase 8
• ephrin type-A receptor 4
• Hek8
• receptor protein-tyrosine kinase HEK8
• SEK
• Sek1
• TYRO1 protein tyrosine kinase
• Tyro1
• Tyrosine-protein kinase receptor SEK
• Tyrosine-protein kinase TYRO1

Background

EphA4, also known as Sek, Sek1, Cek8, Hek8, and Tyro1 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA4 has been shown to bind ephrin-A5, ephrin-A1, ephrin‑A3, ephrin-A2, ephrin-B2, ephrin-B3, and ephrin-A4 (2, 3). The extracellular domains of mouse and human EphA4 share greater than 95% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

1. Eph Nomenclature Committee [letter] (1997) Cell 9:403.
2. Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
3. Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EphA4 Antibody (AF641)(8)

Publications using AF641

• AE Lewis, A Kuwahara, J Franzosi, JO Bush Tracheal separation is driven by NKX2-1-mediated repression of Efnb2 and regulation of endodermal cell sorting Cell Reports, 2022;38(11):110510. 2022 [PMID: 35294885] (IF, Mouse)
• JM Collinson, NO Lindström, C Neves, K Wallace, C Meharg, RH Charles, ZK Ross, AM Fraser, I Mbogo, K Oras, M Nakamoto, S Barker, S Duce, Z Miedzybrod, N Vargesson The developmental and genetic basis of 'clubfoot' in the peroneal muscular atrophy mutant mouse Development, 2018;145(3):. 2018 [PMID: 29439133] (IHC, Mouse)
• KL Todd, KL Baker, MB Eastman, FW Kolling, AG Trausch, CE Nelson, JC Conover EphA4 regulates neuroblast and astrocyte organization in a neurogenic niche J. Neurosci, 2017;0(0):. 2017 [PMID: 28258169] (IHC, Mouse)
• Kao T, Nicholl G, Johansen J, Kania A, Beg A alpha2-chimaerin is required for Eph receptor-class-specific spinal motor axon guidance and coordinate activation of antagonistic muscles. J Neurosci, 2015;35(6):2344-57. 2015 [PMID: 25673830] (Western Blot, Mouse)
• Raft S, Andrade L, Shao D, Akiyama H, Henkemeyer M, Wu D Ephrin-B2 governs morphogenesis of endolymphatic sac and duct epithelia in the mouse inner ear. Dev Biol, 2014;390(1):51-67. 2014 [PMID: 24583262] (IHC, Mouse)
• Kawano H, Katayama Y, Minagawa K, Shimoyama M, Henkemeyer M, Matsui T A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition. Eur. J. Immunol., 2012;42(6):1562-72. 2012 [PMID: 22622783] (Immunoprecipitation, Mouse)
• Kuwako K, Kakumoto K, Imai T Neural RNA-binding protein Musashi1 controls midline crossing of precerebellar neurons through posttranscriptional regulation of Robo3/Rig-1 expression. Neuron, 2010;67(3):407-21. 2010 [PMID: 20696379] (IHC-Fr, Mouse)
• Murai KK, Nguyen LN, Irie F, Yamaguchi Y, Pasquale EB Control of hippocampal dendritic spine morphology through ephrin-A3/EphA4 signaling. Nat. Neurosci., 2003;6(2):153-60. 2003 [PMID: 12496762] (IHC-Fr, Mouse)

Bioinformatics

• Gene Symbol: EPHA4
• Uniprot:
  • Q03137()