EphB6 Antibody


Western blot shows lysates of mouse thymus tissue and mouse brain tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Mouse EphB6 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF611) followed by ...read more

Product Details

Reactivity MuSpecies Glossary
Applications WB

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
View Available Formulations
Catalog# & Formulation Size Price

EphB6 Antibody Summary

Mouse myeloma cell line NS0-derived recombinant mouse EphB6
Accession # O08644
Detects mouse EphB6 in direct ELISAs and Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse (rm) EphB2, rmEphB3, and rmEphB4 is observed.
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1 ug/mL
Read Publications using
AF611 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EphB6 Antibody

  • EC
  • EPH receptor B6
  • EphB6
  • ephrin type-B receptor 6
  • Hep
  • HEPTyrosine-protein kinase-defective receptor EPH-6
  • Mep
  • MGC129910
  • MGC129911


EphB6, also known as Mep (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. However, it has been found that EphB6 contains substitutions within the kinase domain which results in EphB6 having no kinase activity (4). The ligands which bind EphB6 are unknown (2, 3). However, we have observed that the ephrin-B1 and ephrin-B2 ligands can bind the immobilized receptor in an ELISA-type assay. The extracellular domains of human and mouse EphB6 share 92% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
  4. Gurniak, C.B. and L.J. Berg (1996) Oncogene 13:777.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
Species: Mu
Applications: CyTOF-ready, Flow, IHC, WB
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
Species: Ca, Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, IP, WB
Species: Hu
Applications: ELISA
Species: Hu, Mu, Rt
Applications: ICC, WB
Species: Hu
Applications: ELISA
Species: Hu
Applications: IHC, IHC-P, PEP-ELISA, WB
Species: Mu
Applications: Bind
Species: Hu, Mu
Applications: CyTOF-ready, Flow, ICC, IHC, Simple Western, WB
Species: Bv, Ca, Eq, Fe, Hu, Mu, Po, Rt
Applications: ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
Species: Hu
Applications: ELISA
Species: Av, Bv, Ma, Hu, Mu, Pm, Rt
Applications: IF, IHC, IHC-Fr, IHC-P, WB
Species: Hu
Applications: CyTOF-ready, Dual ISH-IHC, ICC, IHC, ICFlow, Simple Western, WB
Species: Hu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-P, WB
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
Species: Bv, Ha, Hu, Mu, Pm, Rt
Applications: B/N, DB, EM, Flow, ICC/IF, IHC, IHC-P, IP, KD, PA, Simple Western, WB
Species: Hu
Applications: BA
Species: Hu
Applications: WB
Species: Hu
Applications: BA

Publications for EphB6 Antibody (AF611)(6)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 6 applications: Cell Culture, Cell Culture Treatment, ICC, IHC, Immunoprecipitation, Western Blot.

Filter By Application
Cell Culture
Cell Culture Treatment
Western Blot
All Applications
Filter By Species
All Species
Showing Publications 1 - 6 of 6.
Publications using AF611 Applications Species
W Shi, B Ye, M Rame, Y Wang, D Ciocca, S Reibel Foi, J Peng, S Qi, N Vitale, H Luo, J Wu The receptor tyrosine kinase EPHB6 regulates catecholamine exocytosis in adrenal gland chromaffin cells J. Biol. Chem., 2020;0(0):. 2020 [PMID: 32321761] (Cell Culture, Mouse) Cell Culture Mouse
W Shi, Y Wang, J Peng, S Qi, N Vitale, N Kaneda, T Murata, H Luo, J Wu EPHB6 controls catecholamine biosynthesis by up-regulating tyrosine hydroxylase transcription in adrenal gland chromaffin cells J. Biol. Chem., 2019;0(0):. 2019 [PMID: 30824540] (Cell Culture Treatment, Mouse) Cell Culture Treatment Mouse
Y Wang, W Shi, A Blanchette, J Peng, S Qi, H Luo, J Ledoux, J Wu EPHB6 and testosterone in concert regulate epinephrine release by adrenal gland chromaffin cells Sci Rep, 2018;8(1):842. 2018 [PMID: 29339804] (ICC, Mouse) ICC Mouse
Kawano H, Katayama Y, Minagawa K, Shimoyama M, Henkemeyer M, Matsui T A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition. Eur. J. Immunol., 2012;42(6):1562-72. 2012 [PMID: 22622783] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Attwood BK, Bourgognon JM, Patel S, Mucha M, Schiavon E, Skrzypiec AE, Young KW, Shiosaka S, Korostynski M, Piechota M, Przewlocki R, Pawlak R Neuropsin cleaves EphB2 in the amygdala to control anxiety. Nature, 2011;473(7347):372-5. 2011 [PMID: 21508957] (Western Blot, Mouse) Western Blot Mouse
Ogawa K, Wada H, Okada N, Harada I, Nakajima T, Pasquale EB, Tsuyama S EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases. J. Cell. Sci., 2006;119(0):559-70. 2006 [PMID: 16443753] (IHC, Mouse) IHC Mouse

Reviews for EphB6 Antibody (AF611) (0)

There are no reviews for EphB6 Antibody (AF611). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for EphB6 Antibody (AF611) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies


Isotype Controls

Additional EphB6 Products

Bioinformatics Tool for EphB6 Antibody (AF611)

Discover related pathways, diseases and genes to EphB6 Antibody (AF611). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for EphB6 Antibody (AF611)

Discover more about diseases related to EphB6 Antibody (AF611).

Pathways for EphB6 Antibody (AF611)

View related products by pathway.

PTMs for EphB6 Antibody (AF611)

Learn more about PTMs related to EphB6 Antibody (AF611).

Blogs on EphB6

There are no specific blogs for EphB6, but you can read our latest blog posts.
Download our IHC Handbook

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our EphB6 Antibody and receive a gift card or discount.


Gene Symbol EPHB6