EphB6 Antibody

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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EphB6 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EphB6
Leu33-Ser587
Accession # O08644
Specificity
Detects mouse EphB6 in direct ELISAs and Western blots. In Western blots, less than 1% cross-reactivity with recombinant mouse (rm) EphB2, rmEphB3, and rmEphB4 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EPHB6
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF611 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EphB6 Antibody

  • EC 2.7.10.1
  • EPH receptor B6
  • EphB6
  • ephrin type-B receptor 6
  • Hep
  • HEPTyrosine-protein kinase-defective receptor EPH-6
  • Mep
  • MGC129910
  • MGC129911

Background

EphB6, also known as Mep (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. However, it has been found that EphB6 contains substitutions within the kinase domain which results in EphB6 having no kinase activity (4). The ligands which bind EphB6 are unknown (2, 3). However, we have observed that the ephrin-B1 and ephrin-B2 ligands can bind the immobilized receptor in an ELISA-type assay. The extracellular domains of human and mouse EphB6 share 92% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
  4. Gurniak, C.B. and L.J. Berg (1996) Oncogene 13:777.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EphB6 Antibody (AF611)(6)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 6 applications: Cell Culture, Cell Culture Treatment, ICC, IHC, Immunoprecipitation, Western Blot.


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Cell Culture
(1)
Cell Culture Treatment
(1)
ICC
(1)
IHC
(1)
Immunoprecipitation
(1)
Western Blot
(1)
All Applications
Filter By Species
Mouse
(6)
All Species
Showing Publications 1 - 6 of 6.
Publications using AF611 Applications Species
W Shi, B Ye, M Rame, Y Wang, D Ciocca, S Reibel Foi, J Peng, S Qi, N Vitale, H Luo, J Wu The receptor tyrosine kinase EPHB6 regulates catecholamine exocytosis in adrenal gland chromaffin cells J. Biol. Chem., 2020;0(0):. 2020 [PMID: 32321761] (Cell Culture, Mouse) Cell Culture Mouse
W Shi, Y Wang, J Peng, S Qi, N Vitale, N Kaneda, T Murata, H Luo, J Wu EPHB6 controls catecholamine biosynthesis by up-regulating tyrosine hydroxylase transcription in adrenal gland chromaffin cells J. Biol. Chem., 2019;0(0):. 2019 [PMID: 30824540] (Cell Culture Treatment, Mouse) Cell Culture Treatment Mouse
Y Wang, W Shi, A Blanchette, J Peng, S Qi, H Luo, J Ledoux, J Wu EPHB6 and testosterone in concert regulate epinephrine release by adrenal gland chromaffin cells Sci Rep, 2018;8(1):842. 2018 [PMID: 29339804] (ICC, Mouse) ICC Mouse
Kawano H, Katayama Y, Minagawa K, Shimoyama M, Henkemeyer M, Matsui T A novel feedback mechanism by Ephrin-B1/B2 in T-cell activation involves a concentration-dependent switch from costimulation to inhibition. Eur. J. Immunol., 2012;42(6):1562-72. 2012 [PMID: 22622783] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Attwood BK, Bourgognon JM, Patel S, Mucha M, Schiavon E, Skrzypiec AE, Young KW, Shiosaka S, Korostynski M, Piechota M, Przewlocki R, Pawlak R Neuropsin cleaves EphB2 in the amygdala to control anxiety. Nature, 2011;473(7347):372-5. 2011 [PMID: 21508957] (Western Blot, Mouse) Western Blot Mouse
Ogawa K, Wada H, Okada N, Harada I, Nakajima T, Pasquale EB, Tsuyama S EphB2 and ephrin-B1 expressed in the adult kidney regulate the cytoarchitecture of medullary tubule cells through Rho family GTPases. J. Cell. Sci., 2006;119(0):559-70. 2006 [PMID: 16443753] (IHC, Mouse) IHC Mouse

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Secondary Antibodies

 

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Other Available Formats

Biotin BAF611

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Bioinformatics

Gene Symbol EPHB6
Uniprot