EphB4 Antibody

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EphB4 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using Goat Anti-Mouse EphB4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF446) at 15 µg/mL overnight at 4 °C. Tissue was ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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EphB4 Antibody Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EphB4
Leu16-Ala539
Accession # P54761
Specificity
Detects mouse EphB4 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) EphB6 and rmEphA3 is observed and less than 1% cross-reactivity with rmEphA2, rmEphA4, rmEphA6, rmEphA7, rmEphA8, rmEphB2, and rmEphB3 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EPHB4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF446 in the following applications:

Publications
Read Publications using
AF446 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EphB4 Antibody

  • EC 2.7.10
  • EC 2.7.10.1
  • EPH receptor B4
  • EphB4
  • ephrin type-B receptor 4
  • hepatoma transmembrane kinase
  • Htk
  • HTKephrin receptor EphB4
  • Mdk2
  • Myk1
  • soluble EPHB4 variant 1
  • soluble EPHB4 variant 2
  • soluble EPHB4 variant 3
  • Tyro11
  • Tyrosine-protein kinase receptor HTK
  • Tyrosine-protein kinase TYRO11

Background

EphB4, also known as Htk, Myk1, Tyro11, and Mdk2 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphB4 has been shown to bind ephrin‑B2 and ephrin‑B1 (2, 3). The extracellular domains of human and mouse EphB4 share 88% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).
  1. Eph Nomenclature Committee [letter] (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for EphB4 Antibody (AF446)(28)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 6 applications: Flow Cytometry, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


Filter By Application
Flow Cytometry
(1)
IHC
(5)
IHC-Fr
(5)
IHC-P
(5)
Immunoprecipitation
(2)
Western Blot
(9)
All Applications
Filter By Species
Human
(3)
Mouse
(23)
Rat
(1)
All Species
Showing Publications 1 - 10 of 28. Show All 28 Publications.
Publications using AF446 Applications Species
G Luxán, J Stewen, N Díaz, K Kato, SK Maney, A Aravamudha, F Berkenfeld, N Nagelmann, HC Drexler, D Zeuschner, C Faber, H Schillers, S Hermann, J Wiseman, JM Vaquerizas, ME Pitulescu, RH Adams Endothelial EphB4 maintains vascular integrity and transport function in adult heart Elife, 2019;8(0):. 2019 [PMID: 31782728] (Western Blot, Human) Western Blot Human
S Payne, M Gunadasa-R, A Neal, AN Redpath, J Patel, KM Chouliaras, I Ratnayaka, N Smart, S De Val Regulatory pathways governing murine coronary vessel formation are dysregulated in the injured adult heart Nat Commun, 2019;10(1):3276. 2019 [PMID: 31332177] (IHC-Fr, Mouse) IHC-Fr Mouse
A Neal, S Nornes, S Payne, MD Wallace, M Fritzsche, P Louphrasit, RN Wilkinson, KM Chouliaras, K Liu, K Plant, R Sholapurka, I Ratnayaka, W Herzog, G Bond, T Chico, G Bou-Ghario, S De Val Venous identity requires BMP signalling through ALK3 Nat Commun, 2019;10(1):453. 2019 [PMID: 30692543] (IHC, Mouse) IHC Mouse
E Groppa, S Brkic, A Uccelli, G Wirth, P Korpisalo-, M Filippova, B Dasen, V Sacchi, MG Muraro, M Trani, S Reginato, R Gianni-Bar, S Ylä-Herttu, A Banfi EphrinB2/EphB4 signaling regulates non-sprouting angiogenesis by VEGF EMBO Rep., 2018;0(0):. 2018 [PMID: 29643120] (IHC-Fr, Mouse) IHC-Fr Mouse
H Toray, T Hasegawa, N Sakagami, E Tsuchiya, A Kudo, S Zhao, Y Moritani, M Abe, T Yoshida, T Yamamoto, T Yamamoto, K Oda, N Udagawa, PH Luiz de Fr, M Li Histochemical assessment for osteoblastic activity coupled with dysfunctional osteoclasts in c-src deficient mice Biomed. Res., 2017;38(2):123-134. 2017 [PMID: 28442663] (IHC, Mouse) IHC Mouse
EphrinB2 repression through ZEB2 mediates tumour invasion and anti-angiogenic resistance Nat Commun, 2016;7(0):12329. 2016 [PMID: 27470974] (Western Blot, Mouse) Western Blot Mouse
Downregulation of proinflammatory cytokines in HTLV-1-infected T cells by Resveratrol J Exp Clin Cancer Res, 2016;35(1):118. 2016 [PMID: 27448598] (IHC-P, Mouse) IHC-P Mouse
EPHB4 kinase-inactivating mutations cause autosomal dominant lymphatic-related hydrops fetalis J Clin Invest, 2016;0(0):. 2016 [PMID: 27400125] (IHC-Fr, Mouse) IHC-Fr Mouse
M Wang, MJ Collins, TR Foster, H Bai, T Hashimoto, JM Santana, C Shu, A Dardik Eph-B4 mediates vein graft adaptation by regulation of endothelial nitric oxide synthase J. Vasc. Surg., 2017;65(1):179-189. 2017 [PMID: 26817610] (Western Blot, Mouse) Western Blot Mouse
Nguyen T, Arthur A, Panagopoulos R, Paton S, Hayball J, Zannettino A, Purton L, Matsuo K, Gronthos S EphB4 Expressing Stromal Cells Exhibit an Enhanced Capacity for Hematopoietic Stem Cell Maintenance. Stem Cells, 2015;33(9):2838-49. 2015 [PMID: 26033476] (Western Blot, Mouse) Western Blot Mouse
Show All 28 Publications.

Reviews for EphB4 Antibody (AF446) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Rat.

Reviews using AF446:
Filter by Applications
WB
(1)
IF
(1)
All Applications
Filter by Species
Rat
(2)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Anonymous
WB Rat 01/08/2015
View

Summary

ApplicationWestern Blot
Sample TestedSee PMID 22398409
SpeciesRat

Blocking

Blocking DetailsSee PMID 22398409

Details

Detection NotesSee PMID 22398409

Comments

CommentsPublished: See PMID 22398409Independent review from antibodyresource.com
  4
reviewed by:
Anonymous
IF Rat 01/08/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22398409
SpeciesRat

Blocking

Blocking DetailsSee PMID 22398409

Details

Detection NotesSee PMID 22398409

Comments

CommentsPublished: See PMID 22398409Independent review from antibodyresource.com

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Secondary Antibodies

 

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Biotin BAF446

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
0

 
Anonymous
01/08/2015
Application: WB
Species: Rat

 
Anonymous
01/08/2015
Application: IF
Species: Rat

Bioinformatics

Gene Symbol EPHB4
Uniprot