Recombinant Human Ephrin-A5 Fc Chimera Protein, CF


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Product Details

Reactivity HuSpecies Glossary
Applications Binding Activity

Order Details

Recombinant Human Ephrin-A5 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to compete with Biotinylated Recombinant Human Ephrin‑A5 Fc Chimera (Catalog # BT374) for binding to immobilized recombinant mouse Eph-A3 Fc Chimera in a functional ELISA assay.
Optimal dilutions should be determined by each laboratory for each application.
Mouse myeloma cell line, NS0-derived human Ephrin-A5 protein
Human Ephrin-A5
Accession # P52803
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Gln21 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Binding Activity
Theoretical MW
48.6 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
50-55 kDa, reducing conditions
Read Publications using
374-EA in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Ephrin-A5 Fc Chimera Protein, CF

  • AF1
  • AL-1
  • EFL-5
  • EFNA5
  • EPH-related receptor tyrosine kinase ligand 7
  • EphrinA5
  • Ephrin-A5
  • EPLG7
  • GLC1M
  • LERK-7
  • RAGS


Ephrin‑A5, also known as AL‑1, RAGS, and LERK‑7, is an approximately 25 kDa member of the Ephrin‑A family of GPI‑anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin‑A ligands are structurally related to the extracellular domains of the transmembrane Ephrin‑B ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). Ephrin‑A5 preferentially interacts with receptors in the EphA family but also with EphB2 (3). Mature human Ephrin‑A5 shares 99.5% aa sequence identity with mouse and rat Ephrin‑A5, respectively (4). Ephrin‑A5 is expressed in multiple tissues during development, particularly in the brain (5, 6). It can exert repulsive or attractive effects on migrating neurons in the developing brain and motor column of the spinal cord (6‑10). Ephrin‑A5 repels migrating axons by inducing growth cone collapse and neurite retraction and by inhibiting the neurotrophic effects of NGF and BDNF (3, 11, 12). It interacts in cis with EphA3 on retinal axon growth cones which reduces axonal sensitivity to Ephrin‑A5 in trans (13). In the adult, Ephrin‑A5 is expressed on hippocampal neurons and astrocytes and induces the development of hippocampal synapses (9, 14, 15). It supports the proliferation of neural progenitors and the survival of newly differentiated neurons (14). Ephrin‑A5 also functions as a tumor suppressor (16). Its normal function in inhibiting EGFR signaling is compromised by its down‑regulation in glioma (16). Ephrin‑A5 is also expressed by muscle precursor cells and interacts with EphA4 to restrict their migration to the correct locations during forelimb morphogenesis (17).
  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Himanen, J.-P. et al. (2004) Nat. Neurosci. 7:501.
  4. Winslow, J.W. et al. (1995) Neuron 14:973.
  5. Deschamps, C. et al. (2010) BMC Neurosci. 11:105.
  6. Cooper, M.A. et al. (2009) Dev. Neurobiol. 69:36.
  7. Frisen, J. et al. (1998) Neuron 20:235.
  8. Zimmer, G. et al. (2008) Eur. J. Neurosci. 28:62.
  9. Otal, R. et al. (2006) Neuroscience 141:109.
  10. Eberhart, J. et al. (2004) J. Neurosci. 24:1070.
  11. Munoz, L.M. et al. (2005) Dev. Biol. 283:397.
  12. Meier, C. et al. (2011) PLoS ONE 6:e26089.
  13. Carvalho, R.F. et al. (2006) Nat. Neurosci. 9:322.
  14. Hara, Y. et al. (2010) Stem Cells 28:974.
  15. Akaneya, Y. et al. (2010) PLoS ONE 5:e12486.
  16. Li, J.-J. et al. (2009) Oncogene 28:1759.
  17. Swartz, M.E. et al. (2001) Development 128:4669.

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Publications for Ephrin-A5 (374-EA)(34)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Chicken, Virus - Henipavirus.

We have publications tested in 7 applications: Bioassay, Coprecipitation Assay, ICC, IHC, IHC-Fr, Immunoprecipitation, In Vivo.

Filter By Application
Coprecipitation Assay
In Vivo
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Virus - Henipavirus
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Showing Publications 1 - 10 of 34. Show All 34 Publications.
Publications using 374-EA Applications Species
SL Banerjee, F Lessard, FJM Chartier, K Jacquet, AI Osornio-He, V Teyssier, K Ghani, N Lavoie, JN Lavoie, M Caruso, P Laprise, S Elowe, JP Lambert, N Bisson EPH receptor tyrosine kinases phosphorylate the PAR-3 scaffold protein to modulate downstream signaling networks Cell Reports, 2022-07-05;40(1):111031. 2022-07-05 [PMID: 35793621] (Bioassay, Human) Bioassay Human
C Cai, M Zhang, L Liu, H Zhang, Y Guo, T Lan, Y Xu, P Ma, S Li ADAM10-cleaved ephrin-A5 contributes to prostate cancer metastasis Cell Death & Disease, 2022-05-12;13(5):453. 2022-05-12 [PMID: 35551177] (Bioassay, Human) Bioassay Human
BA Creighton, S Afriyie, D Ajit, CR Casingal, KM Voos, J Reger, AM Burch, E Dyne, J Bay, JK Huang, ES Anton, MM Fu, DN Lorenzo Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A Elife, 2021-11-23;10(0):. 2021-11-23 [PMID: 34812142] (Bioassay, Human) Bioassay Human
L Teo, AG Boghdadi, J Homman-Lud, IC Mundinano, WC Kwan, JA Bourne Replicating infant-specific reactive astrocyte functions in the injured adult brain Progress in neurobiology, 2021-06-17;0(0):102108. 2021-06-17 [PMID: 34147584] (Bioassay, Human) Bioassay Human
TP Light, M Gomez-Sole, Z Wang, K Karl, E Zapata-Mer, MP Gehring, BC Lechtenber, TV Pogorelov, K Hristova, EB Pasquale A cancer mutation promotes EphA4 oligomerization and signaling by altering the conformation of the SAM domain The Journal of Biological Chemistry, 2021-06-15;297(1):100876. 2021-06-15 [PMID: 34139238] (Bioassay, Human) Bioassay Human
J Jukonen, L Moyano-Gal, K Höpfner, EA Pietilä, L Lehtinen, K Huhtinen, E Gucciardo, J Hynninen, S Hietanen, S Grénman, PM Ojala, O Carpén, K Lehti Aggressive and recurrent ovarian cancers upregulate ephrinA5, a non-canonical effector of EphA2 signaling duality Scientific Reports, 2021-04-23;11(1):8856. 2021-04-23 [PMID: 33893375] (Bioassay, Human) Bioassay Human
YL Chen, YC Yen, CW Jang, SH Wang, HT Huang, CH Chen, JR Hsiao, JY Chang, YW Chen Ephrin A4-ephrin receptor A10 signaling promotes cell migration and spheroid formation by upregulating NANOG expression in oral squamous cell carcinoma cells Scientific Reports, 2021-01-12;11(1):644. 2021-01-12 [PMID: 33436772] (Human) Human
T Verheyen, T Fang, D Lindenhofe, Y Wang, K Akopyan, A Lindqvist, B Högberg, AI Teixeira Spatial�organization-dependent EphA2 transcriptional responses revealed by ligand nanocalipers Nucleic Acids Res., 2020-06-04;0(0):. 2020-06-04 [PMID: 32352518] (Bioassay, Human) Bioassay Human
ED Laing, CK Navaratnar, S Cheliout D, SR Petzing, Y Xu, SL Sterling, GA Marsh, LF Wang, M Amaya, DB Nikolov, R Cattaneo, CC Broder, K Xu Structural and functional analyses reveal promiscuous and species specific use of ephrin receptors by Cedar virus Proc. Natl. Acad. Sci. U.S.A., 2019-09-23;0(0):. 2019-09-23 [PMID: 31548390] (Coprecipitation Assay, Virus - Henipavirus) Coprecipitation Assay Virus - Henipavirus
DM Habiel, MS Espindola, IC Jones, AL Coelho, B Stripp, CM Hogaboam CCR10+ epithelial cells from idiopathic pulmonary fibrosis lungs drive remodeling JCI Insight, 2018-08-23;3(16):. 2018-08-23 [PMID: 30135312] (Bioassay, Human) Bioassay Human
Show All 34 Publications.

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Gene Symbol EFNA5