Recombinant Human Ephrin-A5 Fc Chimera Protein, CF

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Summary
Reactivity HuSpecies Glossary
Applications Bind
Format
Carrier-Free

Order Details

Recombinant Human Ephrin-A5 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its ability to compete with Biotinylated Recombinant Human Ephrin‑A5 Fc Chimera (Catalog # BT374) for binding to immobilized recombinant mouse Eph-A3 Fc Chimera in a functional ELISA assay.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived
Human Ephrin-A5
(Gln21-Asn203)
Accession # P52803
IEGRMD Human IgG1
(Pro100-Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
No results obtained: Gln21 predicted
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
EFNA5
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
48.6 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
50-55 kDa, reducing conditions
Publications
Read Publications using
374-EA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Ephrin-A5 Fc Chimera Protein, CF

  • AF1
  • AL-1
  • EFL-5
  • EFNA5
  • EPH-related receptor tyrosine kinase ligand 7
  • EphrinA5
  • Ephrin-A5
  • EPLG7
  • GLC1M
  • LERK-7
  • LERK7EFL5
  • RAGS

Background

Ephrin‑A5, also known as AL‑1, RAGS, and LERK‑7, is an approximately 25 kDa member of the Ephrin‑A family of GPI‑anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin‑A ligands are structurally related to the extracellular domains of the transmembrane Ephrin‑B ligands. Eph‑Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). Ephrin‑A5 preferentially interacts with receptors in the EphA family but also with EphB2 (3). Mature human Ephrin‑A5 shares 99.5% aa sequence identity with mouse and rat Ephrin‑A5, respectively (4). Ephrin‑A5 is expressed in multiple tissues during development, particularly in the brain (5, 6). It can exert repulsive or attractive effects on migrating neurons in the developing brain and motor column of the spinal cord (6‑10). Ephrin‑A5 repels migrating axons by inducing growth cone collapse and neurite retraction and by inhibiting the neurotrophic effects of NGF and BDNF (3, 11, 12). It interacts in cis with EphA3 on retinal axon growth cones which reduces axonal sensitivity to Ephrin‑A5 in trans (13). In the adult, Ephrin‑A5 is expressed on hippocampal neurons and astrocytes and induces the development of hippocampal synapses (9, 14, 15). It supports the proliferation of neural progenitors and the survival of newly differentiated neurons (14). Ephrin‑A5 also functions as a tumor suppressor (16). Its normal function in inhibiting EGFR signaling is compromised by its down‑regulation in glioma (16). Ephrin‑A5 is also expressed by muscle precursor cells and interacts with EphA4 to restrict their migration to the correct locations during forelimb morphogenesis (17).

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Publications for (20)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Chicken.

We have publications tested in 6 applications: Bioassay, ICC, IHC, IHC Frozen, IP, In Vivo.


Filter By Application
Bioassay
(13)
ICC
(1)
IHC
(1)
IHC Frozen
(2)
IP
(1)
In Vivo
(1)
All Applications
Filter By Species
Human
(6)
Mouse
(9)
Rat
(4)
Chicken
(1)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using 374-EA Applications Species
Anks1a regulates COPII-mediated anterograde transport of receptor tyrosine kinases critical for tumorigenesis Nat Commun, 2016;7(0):12799. 2016 [PMID: 27619642] (Bioassay, Mouse) Bioassay Mouse
Maury Y, Come J, Piskorowski R, Salah-Mohellibi N, Chevaleyre V, Peschanski M, Martinat C, Nedelec S Combinatorial analysis of developmental cues efficiently converts human pluripotent stem cells into multiple neuronal subtypes. Nat Biotechnol, 2015;33(1):89-96. 2015 [PMID: 25383599] (Bioassay, Human) Bioassay Human
Schaupp A, Sabet O, Dudanova I, Ponserre M, Bastiaens P, Klein R The composition of EphB2 clusters determines the strength in the cellular repulsion response. J Cell Biol, 2014;204(3):409-22. 2014 [PMID: 24469634] (Bioassay, Human) Bioassay Human
Szepietowska, Barbara, Horvath, Tamas L, Sherwin, Robert S Role of synaptic plasticity and EphA5-ephrinA5 interaction within the ventromedial hypothalamus in response to recurrent hypoglycemia. Diabetes, 2014;63(3):1140-7. 2014 [PMID: 24222347] (In Vivo, Rat) In Vivo Rat
Defourny J, Poirrier A, Lallemend F, Mateo Sanchez S, Neef J, Vanderhaeghen P, Soriano E, Peuckert C, Kullander K, Fritzsch B, Nguyen L, Moonen G, Moser T, Malgrange B Ephrin-A5/EphA4 signalling controls specific afferent targeting to cochlear hair cells. Nat Commun, 2013;4(0):1438. 2013 [PMID: 23385583] (Bioassay, Mouse) Bioassay Mouse
Szepietowska B, Zhu W, Czyzyk J, Eid T, Sherwin R EphA5-EphrinA5 interactions within the ventromedial hypothalamus influence counterregulatory hormone release and local glutamine/glutamate balance during hypoglycemia. Diabetes, 2013;62(4):1282-8. 2013 [PMID: 23274893] (Rat) Rat
Park, E, Kim, Y, Noh, H, Lee, H, Yoo, S, Park, S EphA/ephrin-A signaling is critically involved in region-specific apoptosis during early brain development. Cell Death Differ, 2013;20(1):169-80. 2013 [PMID: 22976838] (Bioassay, Mouse) Bioassay Mouse
Wilson TR, Fridlyand J, Yan Y, Penuel E, Burton L, Chan E, Peng J, Lin E, Wang Y, Sosman J, Ribas A, Li J, Moffat J, Sutherlin DP, Koeppen H, Merchant M, Neve R, Settleman J Widespread potential for growth-factor-driven resistance to anticancer kinase inhibitors. Nature, 2012;487(7408):505-9. 2012 [PMID: 22763448] (Bioassay, Human) Bioassay Human
Genander M, Holmberg J, Frisen J Ephrins negatively regulate cell proliferation in the epidermis and hair follicle. Stem Cells, 2010;28(7):1196-205. 2010 [PMID: 20506314] (IHC Frozen, Mouse) IHC Frozen Mouse
Afshari FT, Kwok JC, Fawcett JW Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling. J. Neurosci., 2010;30(12):4246-55. 2010 [PMID: 20335460] (Bioassay, Rat) Bioassay Rat
Show All 20 Publications.

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Bioinformatics

Gene Symbol EFNA5
Entrez
Uniprot