Recombinant Mouse Ephrin-A1 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity MuSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Mouse Ephrin-A1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized rmEphA2/Fc Chimera at 2 µg/mL (100 µL/well) can bind rmEphrin-A1 Fc Chimera with a linear range of 0.16-10 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse Ephrin-A1 protein
Mouse Ephrin-A1
(Asp19 - Ser182)
Accession # P52793
IEGRMD Human IgG1
(Pro100 - Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Asp19
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Efna1
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
46.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
50-55 kDa, reducing conditions
Publications
Read Publications using
602-A1 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS.
Purity
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Ephrin-A1 Fc Chimera Protein, CF

  • B61
  • ECKLG
  • EFL1
  • EFL-1
  • EFNA1
  • EPH-related receptor tyrosine kinase ligand 1
  • EphrinA1
  • Ephrin-A1
  • EPLG1TNF alpha-induced protein 4
  • Immediate early response protein B61
  • LERK1
  • LERK1LERK-1
  • ligand of eph-related kinase 1
  • TNFAIP4B61
  • Tumor necrosis factor alpha-induced protein 4
  • tumor necrosis factor, alpha-induced protein 4

Background

Ephrin-A1, also known as B61 and LERK-1, is a member of the Ephrin-A family of GPI-anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin-A ligands are structurally related to the extracellular domains of the transmembrane Ephrin-B ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). Mouse Ephrin-A1 is synthesized with an 17 amino acid (aa) signal peptide, a 165 aa mature chain, and a 23 aa C‑terminal propeptide which is removed prior to GPI linkage of Ephrin-A1 to the membrane (3, 4). It can also be released as a soluble molecule (3, 5, 6). The mature 21 ‑ 25 kDa mouse Ephrin-A1 shares 85% and 94% aa sequence identity with human and rat Ephrin-A1, respectively. Ephrin-A1 is widely expressed on endothelial and epithelial cells, particularly in the lung, intestine, liver, and skin (4, 8). It is expressed on resting CD4+ T cells but is down‑regulated following activation (7, 8). Ligation of Ephrin-A1 on CD4+ T cells inhibits cell proliferation and activation, although soluble Ephrin-A1 can promote T cell chemotaxis (7, 8). In cancer, Ephrin‑A1 is expressed by tumor cells as well as on the tumor-associated vasculature (5, 6, 9). It inhibits tumor cell proliferation and migration but also supports tumor growth by promoting angiogenesis (10 - 12). Soluble Ephrin-A1 additionally promotes neuronal survival and neurite extension (13).
  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Takahashi, H. and T. Ikeda (1995) Oncogene 11:879.
  4. Shao, H. et al. (1995) J. Biol. Chem. 270:5636.
  5. Easty, D.J. et al. (1995) Cancer Res. 55:2528.
  6. Cui, X.-D. et al. (2010) Int. J. Cancer 126:940.
  7. Wohlfahrt, J.G. et al. (2004) J. Immunol. 172:843.
  8. Aasheim, H.-C. et al. (2005) Blood 105:2869.
  9. Ogawa, K. et al. (2000) Oncogene 19:6043.
  10. Liu, D.-P. et al. (2007) Int. J. Oncol. 30:865.
  11. Brantley-Sieders, D.M. et al. (2006) Cancer Res. 66:10315.
  12. Pandey, A. et al. (1995) Science 268:567.
  13. Magal, E. et al. (1996) J. Neurosci. Res. 43:735.

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602-A1
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Publications for Ephrin-A1 (602-A1)(16)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 4 applications: Binding Assay, Bioassay, In Vivo, Whole Cell ELISA Capture.


Filter By Application
Binding Assay
(1)
Bioassay
(12)
In Vivo
(1)
Whole Cell ELISA Capture
(1)
All Applications
Filter By Species
Human
(8)
Mouse
(5)
Rat
(1)
Canine
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using 602-A1 Applications Species
Barquilla A, Lamberto I, Heynen-Genel S, Brill L, Pasquale E Protein kinase A can block EphA2 receptor-mediated cell repulsion by increasing EphA2 S897 phosphorylation. Mol Biol Cell, 0;27(17):2757-70. 0 [PMID: 27385333] (Bioassay, Human) Bioassay Human
Kobayashi H, Saito T, Sato K, Furusawa K, Hosokawa T, Tsutsumi K, Asada A, Kamada S, Ohshima T, Hisanaga S Phosphorylation of cyclin-dependent kinase 5 (Cdk5) at Tyr-15 is inhibited by Cdk5 activators and does not contribute to the activation of Cdk5. J Biol Chem, 2014;289(28):19627-36. 2014 [PMID: 24872417] (Bioassay, Mouse) Bioassay Mouse
Naudin C, Sirvent A, Leroy C, Larive R, Simon V, Pannequin J, Bourgaux J, Pierre J, Robert B, Hollande F, Roche S SLAP displays tumour suppressor functions in colorectal cancer via destabilization of the SRC substrate EPHA2. Nat Commun, 2014;5(0):3159. 2014 [PMID: 24457997]
Ieguchi K, Tomita T, Omori T, Komatsu A, Deguchi A, Masuda J, Duffy S, Coulthard M, Boyd A, Maru Y ADAM12-cleaved ephrin-A1 contributes to lung metastasis. Oncogene, 2015;33(17):2179-90. 2015 [PMID: 23686306] (Bioassay, Human) Bioassay Human
Giorgio C, Hassan Mohamed I, Flammini L, Barocelli E, Incerti M, Lodola A, Tognolini M Lithocholic acid is an Eph-ephrin ligand interfering with Eph-kinase activation. PLoS ONE, 2011;6(3):e18128. 2011 [PMID: 21479221] (Whole Cell ELISA Capture, Human) Whole Cell ELISA Capture Human
Dunaway CM, Hwang Y, Lindsley CW Cooperative signaling between Slit2 and Ephrin-A1 regulates a balance between angiogenesis and angiostasis. Mol. Cell. Biol., 2011;31(3):404-16. 2011 [PMID: 21135133] (In Vivo, Mouse) In Vivo Mouse
Afshari FT, Kwok JC, Fawcett JW Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling. J. Neurosci., 2010;30(12):4246-55. 2010 [PMID: 20335460] (Bioassay, Rat) Bioassay Rat
Miura K, Nam JM, Kojima C, Mochizuki N, Sabe H EphA2 engages Git1 to suppress Arf6 activity modulating epithelial cell-cell contacts. Mol. Biol. Cell, 2009;20(7):1949-59. 2009 [PMID: 19193766] (Bioassay, Canine) Bioassay Canine
Wykosky J, Palma E, Gibo DM Soluble monomeric EphrinA1 is released from tumor cells and is a functional ligand for the EphA2 receptor. Oncogene, 2008;27(58):7260-73. 2008 [PMID: 18794797] (Bioassay, Human) Bioassay Human
Yamashita T, Ohneda K, Nagano M, Miyoshi C, Kaneko N, Miwa Y, Yamamoto M, Ohneda O, Fujii-Kuriyama Y Hypoxia-inducible transcription factor-2alpha in endothelial cells regulates tumor neovascularization through activation of ephrin A1. J. Biol. Chem., 2008;283(27):18926-36. 2008 [PMID: 18434321] (Bioassay, Mouse) Bioassay Mouse
Show All 16 Publications.

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Bioinformatics

Gene Symbol Efna1
Entrez
Uniprot