Recombinant Mouse Ephrin-A1 Fc Chimera Protein, CF

• Reactivity: Mu
• Applications: Binding Activity
• Format: Carrier-Free

Recombinant Mouse Ephrin-A1 Fc Chimera Protein, CF Summary

• Details of Functionality: Measured by its binding ability in a functional ELISA. Immobilized rmEphA2/Fc Chimera at 2 µg/mL (100 µL/well) can bind rmEphrin-A1 Fc Chimera with a linear range of 0.16-10 ng/mL.
• Source: Mouse myeloma cell line, NS0-derived mouse Ephrin-A1 protein
  

  Mouse Ephrin-A1 (Asp19 - Ser182) Accession # P52793	IEGRMD	Human IgG1 (Pro100 - Lys330)	6-His tag
  N-terminus			C-terminus

• Accession #: P52793
• N-terminal Sequence: Asp19
• Structure / Form: Disulfide-linked homodimer
• Protein/Peptide Type: Recombinant Proteins
• Gene: Efna1
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Endotoxin Note: <0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

• Dilutions:
  • Binding Activity
• Theoretical MW: 46.8 kDa (monomer).
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• SDS-PAGE: 50-55 kDa, reducing conditions

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain
• Reconstitution Instructions: Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Ephrin-A1 Fc Chimera Protein, CF

• B61
• ECKLG
• EFL1
• EFL-1
• EFNA1
• EPH-related receptor tyrosine kinase ligand 1
• EphrinA1
• Ephrin-A1
• EPLG1TNF alpha-induced protein 4
• Immediate early response protein B61
• LERK1
• LERK1LERK-1
• ligand of eph-related kinase 1
• TNFAIP4B61
• Tumor necrosis factor alpha-induced protein 4
• tumor necrosis factor, alpha-induced protein 4

Background

Ephrin-A1, also known as B61 and LERK-1, is a member of the Ephrin-A family of GPI-anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin-A ligands are structurally related to the extracellular domains of the transmembrane Ephrin-B ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). Mouse Ephrin-A1 is synthesized with an 17 amino acid (aa) signal peptide, a 165 aa mature chain, and a 23 aa C‑terminal propeptide which is removed prior to GPI linkage of Ephrin-A1 to the membrane (3, 4). It can also be released as a soluble molecule (3, 5, 6). The mature 21 ‑ 25 kDa mouse Ephrin-A1 shares 85% and 94% aa sequence identity with human and rat Ephrin-A1, respectively. Ephrin-A1 is widely expressed on endothelial and epithelial cells, particularly in the lung, intestine, liver, and skin (4, 8). It is expressed on resting CD4+ T cells but is down‑regulated following activation (7, 8). Ligation of Ephrin-A1 on CD4+ T cells inhibits cell proliferation and activation, although soluble Ephrin-A1 can promote T cell chemotaxis (7, 8). In cancer, Ephrin‑A1 is expressed by tumor cells as well as on the tumor-associated vasculature (5, 6, 9). It inhibits tumor cell proliferation and migration but also supports tumor growth by promoting angiogenesis (10 - 12). Soluble Ephrin-A1 additionally promotes neuronal survival and neurite extension (13).

1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
3. Takahashi, H. and T. Ikeda (1995) Oncogene 11:879.
4. Shao, H. et al. (1995) J. Biol. Chem. 270:5636.
5. Easty, D.J. et al. (1995) Cancer Res. 55:2528.
6. Cui, X.-D. et al. (2010) Int. J. Cancer 126:940.
7. Wohlfahrt, J.G. et al. (2004) J. Immunol. 172:843.
8. Aasheim, H.-C. et al. (2005) Blood 105:2869.
9. Ogawa, K. et al. (2000) Oncogene 19:6043.
10. Liu, D.-P. et al. (2007) Int. J. Oncol. 30:865.
11. Brantley-Sieders, D.M. et al. (2006) Cancer Res. 66:10315.
12. Pandey, A. et al. (1995) Science 268:567.
13. Magal, E. et al. (1996) J. Neurosci. Res. 43:735.

Publications for Ephrin-A1 (602-A1)(23)

Publications using 602-A1

• Koutouan, E;Niibe, A;Sample, JW;Carlson, D;Graham, R;Smoot, RL;Pasquale, EB; Frequent EPHA2 receptor mutations in cholangiocarcinoma disrupt receptor forward signaling supporting a tumor suppressor role bioRxiv : the preprint server for biology 2025-02-28 [PMID: 40060438] (Bioassay, Immunoprecipitation, Human)
• Matsumoto, M;Gomez-Soler, M;Lombardi, S;Lechtenberg, BC;Pasquale, EB; Missense Mutations of the Ephrin Receptor EPHA1 Associated with Alzheimer's Disease Disrupt Receptor Signaling Functions The Journal of biological chemistry 2024-12-18 [PMID: 39706267] (Bioassay, Human)
• L Zhang, C Zhou, Y Zhou, W Zhang, X Hu, M Chen, H Hui, L Guo, C Wu, J Zhou, H Yin P-Y/G@NHs sensitizes non-small cell lung cancer cells to radiotherapy via blockage of the PI3K/AKT signaling pathway Bioorganic chemistry, 2022-12-06;131(0):106317. 2022-12-06 [PMID: 36525920] (Bioassay, Mouse)
• MP Gehring, EB Pasquale Protein kinase C phosphorylates the EphA2 receptor on serine 892 in the regulatory linker connecting the kinase and SAM domains Cell. Signal., 2020-05-13;73(0):109668. 2020-05-13 [PMID: 32413552] (Bioassay, Human)
• TK Darling, PN Mimche, C Bray, B Umaru, LM Brady, C Stone, CE Eboumbou M, TE Lane, LS Ayong, TJ Lamb EphA2 contributes to disruption of the blood-brain barrier in cerebral malaria PLoS Pathog., 2020-01-30;16(1):e1008261. 2020-01-30 [PMID: 31999807] (Bioassay, Mouse)
• ED Laing, CK Navaratnar, S Cheliout D, SR Petzing, Y Xu, SL Sterling, GA Marsh, LF Wang, M Amaya, DB Nikolov, R Cattaneo, CC Broder, K Xu Structural and functional analyses reveal promiscuous and species specific use of ephrin receptors by Cedar virus Proc. Natl. Acad. Sci. U.S.A., 2019-09-23;0(0):. 2019-09-23 [PMID: 31548390] (Coprecipitation Assay, Virus - Henipavirus)
• Barquilla A, Lamberto I, Heynen-Genel S, Brill L, Pasquale E Protein kinase A can block EphA2 receptor-mediated cell repulsion by increasing EphA2 S897 phosphorylation. Mol Biol Cell, 2016-07-06;27(17):2757-70. 2016-07-06 [PMID: 27385333] (Bioassay, Human)
• Kobayashi H, Saito T, Sato K, Furusawa K, Hosokawa T, Tsutsumi K, Asada A, Kamada S, Ohshima T, Hisanaga S Phosphorylation of cyclin-dependent kinase 5 (Cdk5) at Tyr-15 is inhibited by Cdk5 activators and does not contribute to the activation of Cdk5. J Biol Chem, 2014-05-28;289(28):19627-36. 2014-05-28 [PMID: 24872417] (Bioassay, Mouse)
• Naudin C, Sirvent A, Leroy C, Larive R, Simon V, Pannequin J, Bourgaux J, Pierre J, Robert B, Hollande F, Roche S SLAP displays tumour suppressor functions in colorectal cancer via destabilization of the SRC substrate EPHA2. Nat Commun, 2014-01-01;5(0):3159. 2014-01-01 [PMID: 24457997] (Bioassay, Human)
• Ieguchi K, Tomita T, Omori T, Komatsu A, Deguchi A, Masuda J, Duffy S, Coulthard M, Boyd A, Maru Y ADAM12-cleaved ephrin-A1 contributes to lung metastasis. Oncogene, 2013-05-20;33(17):2179-90. 2013-05-20 [PMID: 23686306] (Bioassay, In Vivo, Human, Mouse)
• Giorgio C, Hassan Mohamed I, Flammini L, Barocelli E, Incerti M, Lodola A, Tognolini M Lithocholic acid is an Eph-ephrin ligand interfering with Eph-kinase activation. PLoS ONE, 2011-03-30;6(3):e18128. 2011-03-30 [PMID: 21479221] (Binding Assay, Human)
• Dunaway CM, Hwang Y, Lindsley CW Cooperative signaling between Slit2 and Ephrin-A1 regulates a balance between angiogenesis and angiostasis. Mol. Cell. Biol., 2010-12-06;31(3):404-16. 2010-12-06 [PMID: 21135133] (In Vivo, Mouse)
• Samantha Lin, Kristin Gordon, Nihal Kaplan, Spiro Getsios Ligand Targeting of EphA2 Enhances Keratinocyte Adhesion and Differentiation via Desmoglein 1 Molecular Biology of the Cell 2010-11-15 [PMID: 20861311] (Bioassay, Human)
• Afshari FT, Kwok JC, Fawcett JW Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling. J. Neurosci., 2010-03-24;30(12):4246-55. 2010-03-24 [PMID: 20335460] (Bioassay, Rat)
• Miura K, Nam JM, Kojima C, Mochizuki N, Sabe H EphA2 engages Git1 to suppress Arf6 activity modulating epithelial cell-cell contacts. Mol. Biol. Cell, 2009-02-04;20(7):1949-59. 2009-02-04 [PMID: 19193766] (Bioassay, Canine)
• Wykosky J, Palma E, Gibo DM Soluble monomeric EphrinA1 is released from tumor cells and is a functional ligand for the EphA2 receptor. Oncogene, 2008-09-15;27(58):7260-73. 2008-09-15 [PMID: 18794797] (Bioassay, Human)
• Yamashita T, Ohneda K, Nagano M, Miyoshi C, Kaneko N, Miwa Y, Yamamoto M, Ohneda O, Fujii-Kuriyama Y Hypoxia-inducible transcription factor-2alpha in endothelial cells regulates tumor neovascularization through activation of ephrin A1. J. Biol. Chem., 2008-04-23;283(27):18926-36. 2008-04-23 [PMID: 18434321] (Bioassay, Mouse)
• Masuda J, Usui R, Maru Y Fibronectin type I repeat is a nonactivating ligand for EphA1 and inhibits ATF3-dependent angiogenesis. J. Biol. Chem., 2008-02-28;283(19):13148-55. 2008-02-28 [PMID: 18308734] (Bioassay, Human)
• Hong S, Kang UJ, Isacson O, Kim KS Neural precursors derived from human embryonic stem cells maintain long-term proliferation without losing the potential to differentiate into all three neural lineages, including dopaminergic neurons. J. Neurochem., 2007-10-18;104(2):316-24. 2007-10-18 [PMID: 17944878] (Bioassay, Mouse)
• Konstantinova I, Nikolova G, Ohara-Imaizumi M, Meda P, Kucera T, Zarbalis K, Wurst W, Nagamatsu S, Lammert E EphA-Ephrin-A-mediated beta cell communication regulates insulin secretion from pancreatic islets. Cell, 2007-04-20;129(2):359-70. 2007-04-20 [PMID: 17448994] (Bioassay, Mouse)
• Chaudhari A, Mahfouz M, Fialho AM, Yamada T, Granja AT, Zhu Y, Hashimoto W, Schlarb-Ridley B, Cho W, Das Gupta TK, Chakrabarty AM Cupredoxin-cancer interrelationship: azurin binding with EphB2, interference in EphB2 tyrosine phosphorylation, and inhibition of cancer growth. Biochemistry, 2007-01-24;46(7):1799-810. 2007-01-24 [PMID: 17249693] (Binding Assay, Human)
• Macrae M, Neve RM, Rodriguez-Viciana P, Haqq C, Yeh J, Chen C, Gray JW, McCormick F A conditional feedback loop regulates Ras activity through EphA2. Cancer Cell, 2005-08-01;8(2):111-8. 2005-08-01 [PMID: 16098464] (Bioassay, Human)
• Sharfe N, Freywald A, Toro A, Dadi H, Roifman C Ephrin stimulation modulates T cell chemotaxis. Eur. J. Immunol., 2002-12-01;32(12):3745-55. 2002-12-01 [PMID: 12516569] (Bioassay, Human)

Bioinformatics

• Gene Symbol: Efna1
• Uniprot:
  • P52793()