Recombinant Mouse Ephrin-A1 Fc Chimera Protein, CF


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Product Details

Reactivity MuSpecies Glossary
Applications Binding Activity

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Recombinant Mouse Ephrin-A1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized rmEphA2/Fc Chimera at 2 µg/mL (100 µL/well) can bind rmEphrin-A1 Fc Chimera with a linear range of 0.16-10 ng/mL.
Mouse myeloma cell line, NS0-derived
Mouse Ephrin-A1
(Asp19 - Ser182)
Accession # P52793
(Pro100 - Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
46.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
50-55 kDa, reducing conditions
Read Publications using
602-A1 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Ephrin-A1 Fc Chimera Protein, CF

  • B61
  • EFL1
  • EFL-1
  • EFNA1
  • EPH-related receptor tyrosine kinase ligand 1
  • EphrinA1
  • Ephrin-A1
  • EPLG1TNF alpha-induced protein 4
  • Immediate early response protein B61
  • LERK1
  • ligand of eph-related kinase 1
  • TNFAIP4B61
  • Tumor necrosis factor alpha-induced protein 4
  • tumor necrosis factor, alpha-induced protein 4


Ephrin-A1, also known as B61 and LERK-1, is a member of the Ephrin-A family of GPI-anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin-A ligands are structurally related to the extracellular domains of the transmembrane Ephrin-B ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). Mouse Ephrin-A1 is synthesized with an 17 amino acid (aa) signal peptide, a 165 aa mature chain, and a 23 aa C‑terminal propeptide which is removed prior to GPI linkage of Ephrin-A1 to the membrane (3, 4). It can also be released as a soluble molecule (3, 5, 6). The mature 21 ‑ 25 kDa mouse Ephrin-A1 shares 85% and 94% aa sequence identity with human and rat Ephrin-A1, respectively. Ephrin-A1 is widely expressed on endothelial and epithelial cells, particularly in the lung, intestine, liver, and skin (4, 8). It is expressed on resting CD4+ T cells but is down‑regulated following activation (7, 8). Ligation of Ephrin-A1 on CD4+ T cells inhibits cell proliferation and activation, although soluble Ephrin-A1 can promote T cell chemotaxis (7, 8). In cancer, Ephrin‑A1 is expressed by tumor cells as well as on the tumor-associated vasculature (5, 6, 9). It inhibits tumor cell proliferation and migration but also supports tumor growth by promoting angiogenesis (10 - 12). Soluble Ephrin-A1 additionally promotes neuronal survival and neurite extension (13).

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Publications for Ephrin-A1 (602-A1)(16)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Canine.

We have publications tested in 4 applications: Binding Assay, Bioassay, In Vivo, Whole Cell ELISA Capture.

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Binding Assay
In Vivo
Whole Cell ELISA Capture
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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using 602-A1 Applications Species
Barquilla A, Lamberto I, Heynen-Genel S, Brill L, Pasquale E Protein kinase A can block EphA2 receptor-mediated cell repulsion by increasing EphA2 S897 phosphorylation. Mol Biol Cell, 0;27(17):2757-70. 0 [PMID: 27385333] (Bioassay, Human) Bioassay Human
Kobayashi H, Saito T, Sato K, Furusawa K, Hosokawa T, Tsutsumi K, Asada A, Kamada S, Ohshima T, Hisanaga S Phosphorylation of cyclin-dependent kinase 5 (Cdk5) at Tyr-15 is inhibited by Cdk5 activators and does not contribute to the activation of Cdk5. J Biol Chem, 2014;289(28):19627-36. 2014 [PMID: 24872417] (Bioassay, Mouse) Bioassay Mouse
Naudin C, Sirvent A, Leroy C, Larive R, Simon V, Pannequin J, Bourgaux J, Pierre J, Robert B, Hollande F, Roche S SLAP displays tumour suppressor functions in colorectal cancer via destabilization of the SRC substrate EPHA2. Nat Commun, 2014;5(0):3159. 2014 [PMID: 24457997]
Ieguchi K, Tomita T, Omori T, Komatsu A, Deguchi A, Masuda J, Duffy S, Coulthard M, Boyd A, Maru Y ADAM12-cleaved ephrin-A1 contributes to lung metastasis. Oncogene, 2015;33(17):2179-90. 2015 [PMID: 23686306] (Bioassay, Human) Bioassay Human
Giorgio C, Hassan Mohamed I, Flammini L, Barocelli E, Incerti M, Lodola A, Tognolini M Lithocholic acid is an Eph-ephrin ligand interfering with Eph-kinase activation. PLoS ONE, 2011;6(3):e18128. 2011 [PMID: 21479221] (Whole Cell ELISA Capture, Human) Whole Cell ELISA Capture Human
Dunaway CM, Hwang Y, Lindsley CW Cooperative signaling between Slit2 and Ephrin-A1 regulates a balance between angiogenesis and angiostasis. Mol. Cell. Biol., 2011;31(3):404-16. 2011 [PMID: 21135133] (In Vivo, Mouse) In Vivo Mouse
Afshari FT, Kwok JC, Fawcett JW Astrocyte-produced ephrins inhibit schwann cell migration via VAV2 signaling. J. Neurosci., 2010;30(12):4246-55. 2010 [PMID: 20335460] (Bioassay, Rat) Bioassay Rat
Miura K, Nam JM, Kojima C, Mochizuki N, Sabe H EphA2 engages Git1 to suppress Arf6 activity modulating epithelial cell-cell contacts. Mol. Biol. Cell, 2009;20(7):1949-59. 2009 [PMID: 19193766] (Bioassay, Canine) Bioassay Canine
Wykosky J, Palma E, Gibo DM Soluble monomeric EphrinA1 is released from tumor cells and is a functional ligand for the EphA2 receptor. Oncogene, 2008;27(58):7260-73. 2008 [PMID: 18794797] (Bioassay, Human) Bioassay Human
Yamashita T, Ohneda K, Nagano M, Miyoshi C, Kaneko N, Miwa Y, Yamamoto M, Ohneda O, Fujii-Kuriyama Y Hypoxia-inducible transcription factor-2alpha in endothelial cells regulates tumor neovascularization through activation of ephrin A1. J. Biol. Chem., 2008;283(27):18926-36. 2008 [PMID: 18434321] (Bioassay, Mouse) Bioassay Mouse
Show All 16 Publications.

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Gene Symbol Efna1