Recombinant Mouse Ephrin-A1 Fc Chimera Protein, CF


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Product Details

Reactivity MuSpecies Glossary
Applications Binding Activity

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Recombinant Mouse Ephrin-A1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized rmEphA2/Fc Chimera at 2 µg/mL (100 µL/well) can bind rmEphrin-A1 Fc Chimera with a linear range of 0.16-10 ng/mL.
Mouse myeloma cell line, NS0-derived mouse Ephrin-A1 protein
Mouse Ephrin-A1
(Asp19 - Ser182)
Accession # P52793
(Pro100 - Lys330)
6-His tag
N-terminus C-terminus
Accession #
N-terminal Sequence
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Binding Activity
Theoretical MW
46.8 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
50-55 kDa, reducing conditions
Read Publications using
602-A1 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS.
>90%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse Ephrin-A1 Fc Chimera Protein, CF

  • B61
  • EFL1
  • EFL-1
  • EFNA1
  • EPH-related receptor tyrosine kinase ligand 1
  • EphrinA1
  • Ephrin-A1
  • EPLG1TNF alpha-induced protein 4
  • Immediate early response protein B61
  • LERK1
  • ligand of eph-related kinase 1
  • TNFAIP4B61
  • Tumor necrosis factor alpha-induced protein 4
  • tumor necrosis factor, alpha-induced protein 4


Ephrin-A1, also known as B61 and LERK-1, is a member of the Ephrin-A family of GPI-anchored ligands that bind and induce the tyrosine autophosphorylation of Eph receptors. Ephrin-A ligands are structurally related to the extracellular domains of the transmembrane Ephrin-B ligands. Eph-Ephrin interactions are widely involved in the regulation of cell migration, tissue morphogenesis, and cancer progression (1, 2). Mouse Ephrin-A1 is synthesized with an 17 amino acid (aa) signal peptide, a 165 aa mature chain, and a 23 aa C‑terminal propeptide which is removed prior to GPI linkage of Ephrin-A1 to the membrane (3, 4). It can also be released as a soluble molecule (3, 5, 6). The mature 21 ‑ 25 kDa mouse Ephrin-A1 shares 85% and 94% aa sequence identity with human and rat Ephrin-A1, respectively. Ephrin-A1 is widely expressed on endothelial and epithelial cells, particularly in the lung, intestine, liver, and skin (4, 8). It is expressed on resting CD4+ T cells but is down‑regulated following activation (7, 8). Ligation of Ephrin-A1 on CD4+ T cells inhibits cell proliferation and activation, although soluble Ephrin-A1 can promote T cell chemotaxis (7, 8). In cancer, Ephrin‑A1 is expressed by tumor cells as well as on the tumor-associated vasculature (5, 6, 9). It inhibits tumor cell proliferation and migration but also supports tumor growth by promoting angiogenesis (10 - 12). Soluble Ephrin-A1 additionally promotes neuronal survival and neurite extension (13).
  1. Miao, H. and B. Wang (2009) Int. J. Biochem. Cell Biol. 41:762.
  2. Pasquale, E.B. (2010) Nat. Rev. Cancer 10:165.
  3. Takahashi, H. and T. Ikeda (1995) Oncogene 11:879.
  4. Shao, H. et al. (1995) J. Biol. Chem. 270:5636.
  5. Easty, D.J. et al. (1995) Cancer Res. 55:2528.
  6. Cui, X.-D. et al. (2010) Int. J. Cancer 126:940.
  7. Wohlfahrt, J.G. et al. (2004) J. Immunol. 172:843.
  8. Aasheim, H.-C. et al. (2005) Blood 105:2869.
  9. Ogawa, K. et al. (2000) Oncogene 19:6043.
  10. Liu, D.-P. et al. (2007) Int. J. Oncol. 30:865.
  11. Brantley-Sieders, D.M. et al. (2006) Cancer Res. 66:10315.
  12. Pandey, A. et al. (1995) Science 268:567.
  13. Magal, E. et al. (1996) J. Neurosci. Res. 43:735.

Publications for Ephrin-A1 (602-A1)(20)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Canine, Virus - Henipavirus.

We have publications tested in 4 applications: Binding Assay, Bioassay, Coprecipitation Assay, In Vivo.

Filter By Application
Binding Assay
Coprecipitation Assay
In Vivo
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Virus - Henipavirus
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using 602-A1 Applications Species
L Zhang, C Zhou, Y Zhou, W Zhang, X Hu, M Chen, H Hui, L Guo, C Wu, J Zhou, H Yin P-Y/G@NHs sensitizes non-small cell lung cancer cells to radiotherapy via blockage of the PI3K/AKT signaling pathway Bioorganic chemistry, 2022-12-06;131(0):106317. 2022-12-06 [PMID: 36525920] (Bioassay, Mouse) Bioassay Mouse
MP Gehring, EB Pasquale Protein kinase C phosphorylates the EphA2 receptor on serine 892 in the regulatory linker connecting the kinase and SAM domains Cell. Signal., 2020-05-13;73(0):109668. 2020-05-13 [PMID: 32413552] (Bioassay, Human) Bioassay Human
TK Darling, PN Mimche, C Bray, B Umaru, LM Brady, C Stone, CE Eboumbou M, TE Lane, LS Ayong, TJ Lamb EphA2 contributes to disruption of the blood-brain barrier in cerebral malaria PLoS Pathog., 2020-01-30;16(1):e1008261. 2020-01-30 [PMID: 31999807] (Bioassay, Mouse) Bioassay Mouse
ED Laing, CK Navaratnar, S Cheliout D, SR Petzing, Y Xu, SL Sterling, GA Marsh, LF Wang, M Amaya, DB Nikolov, R Cattaneo, CC Broder, K Xu Structural and functional analyses reveal promiscuous and species specific use of ephrin receptors by Cedar virus Proc. Natl. Acad. Sci. U.S.A., 2019-09-23;0(0):. 2019-09-23 [PMID: 31548390] (Coprecipitation Assay, Virus - Henipavirus) Coprecipitation Assay Virus - Henipavirus
Barquilla A, Lamberto I, Heynen-Genel S, Brill L, Pasquale E Protein kinase A can block EphA2 receptor-mediated cell repulsion by increasing EphA2 S897 phosphorylation. Mol Biol Cell, 2016-07-06;27(17):2757-70. 2016-07-06 [PMID: 27385333] (Bioassay, Human) Bioassay Human
Kobayashi H, Saito T, Sato K, Furusawa K, Hosokawa T, Tsutsumi K, Asada A, Kamada S, Ohshima T, Hisanaga S Phosphorylation of cyclin-dependent kinase 5 (Cdk5) at Tyr-15 is inhibited by Cdk5 activators and does not contribute to the activation of Cdk5. J Biol Chem, 2014-05-28;289(28):19627-36. 2014-05-28 [PMID: 24872417] (Bioassay, Mouse) Bioassay Mouse
Naudin C, Sirvent A, Leroy C, Larive R, Simon V, Pannequin J, Bourgaux J, Pierre J, Robert B, Hollande F, Roche S SLAP displays tumour suppressor functions in colorectal cancer via destabilization of the SRC substrate EPHA2. Nat Commun, 2014-01-01;5(0):3159. 2014-01-01 [PMID: 24457997] (Bioassay, Human) Bioassay Human
Ieguchi K, Tomita T, Omori T, Komatsu A, Deguchi A, Masuda J, Duffy S, Coulthard M, Boyd A, Maru Y ADAM12-cleaved ephrin-A1 contributes to lung metastasis. Oncogene, 2013-05-20;33(17):2179-90. 2013-05-20 [PMID: 23686306] (Bioassay, In Vivo, Human, Mouse) Bioassay, In Vivo Human, Mouse
Giorgio C, Hassan Mohamed I, Flammini L, Barocelli E, Incerti M, Lodola A, Tognolini M Lithocholic acid is an Eph-ephrin ligand interfering with Eph-kinase activation. PLoS ONE, 2011-03-30;6(3):e18128. 2011-03-30 [PMID: 21479221] (Binding Assay, Human) Binding Assay Human
Dunaway CM, Hwang Y, Lindsley CW Cooperative signaling between Slit2 and Ephrin-A1 regulates a balance between angiogenesis and angiostasis. Mol. Cell. Biol., 2010-12-06;31(3):404-16. 2010-12-06 [PMID: 21135133] (In Vivo, Mouse) In Vivo Mouse
Show All 20 Publications.

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Gene Symbol Efna1