EphA2 Antibody [Unconjugated]

Images

 
Western blot shows lysates of A431 human epithelial carcinoma parental cell line and EphA2 knock out (KO) A431 cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human EphA2 Antigen Affinity-purified ...read more
A431 human epithelial carcinoma cell line was stained with Goat Anti-Human EphA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3035, filled histogram) or isotype control antibody (Catalog # AB-108-C, open ...read more
EphA2 was detected in immersion fixed paraffin-embedded sections of human ovarian cancer tissue using Goat Anti-Human EphA2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3035) at 5 µg/mL overnight at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, IHC, CyTOF-ready, KO
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

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EphA2 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human EphA2
Gln25-Asn534
Accession # P29317
Specificity
Detects human EphA2 in direct ELISAs and Western blots. In direct ELISAs, less than 45% cross-reactivity with recombinant mouse EphA2 is observed and less than 1% cross-reactivity with recombinant human (rh) EphA1, rhEphA3, rhEphA4, rhEphA5, rhEphA6, rhEphA7 and rhEphA10 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
EPHA2
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • Knockout Validated 1 ug/mL
  • Western Blot 1 ug/mL
Publications
Read Publications using
AF3035 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for EphA2 Antibody [Unconjugated]

  • ARCC2
  • EC 2.7.10
  • EC 2.7.10.1
  • Eck
  • ECKepithelial cell receptor protein tyrosine kinase
  • EPH receptor A2
  • EphA2
  • ephrin type-A receptor 2
  • Epithelial cell kinase
  • Myk2
  • Sek2
  • soluble EPHA2 variant 1
  • Tyrosine-protein kinase receptor ECK

Background

EphA2, also known as Eck, Myk2, and Sek2, is a member of the Eph receptor tyrosine kinase family which binds Ephrins A1, 2, 3, 4, and 5 (1-4). A and B class Eph proteins have a common structural organization. The human EphA2 cDNA encodes a 976 amino acid (aa) precursor including a 24 aa signal sequence, a 510 aa extracellular domain (ECD), a 24 aa transmembrane segment, and a 418 aa cytoplasmic domain. The ECD contains an N-terminal globular domain, a cysteine-rich domain, and two fibronectin type III domains (5). The cytoplasmic domain contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a sterile alpha motif (SAM) (5). The ECD of human EphA2 shares 90-94% aa sequence identity with mouse, bovine, and canine EphA2, and approximately 45% aa sequence identity with human EphA1, 3, 4, 5, 7, and 8. EphA2 becomes autophosphorylated following ligand binding (6, 7) and then interacts with SH2 domain-containing PI3-kinase to activate MAPK pathways (8, 9). Reverse signaling is also propagated through the Ephrin ligand. Transcription of EphA2 is dependent on the expression of E-Cadherin (10), and can be induced by p53 family transcription factors (11). EphA2 is upregulated in breast, prostate, and colon cancer vascular endothelium. Its ligand, EphrinA1, is expressed by the local tumor cells (12, 13). In some cases, EphA2 and EphrinA1 are expressed on the same blood vessels (14). EphA2 signaling cooperates with VEGF receptor signaling in promoting endothelial cell migration (13). The gene encoding human EphA2 maps to a region on chromosome 1 which is frequently deleted in neuroectodermal tumors (15).

  1. Poliakov, A. et al. (2004) Dev. Cell 7:465.
  2. Surawska, H. et al. (2004) Cytokine Growth Factor Rev. 15:419. 
  3. Pasquale, E.B. (2005) Nat. Rev. Mol. Cell Biol. 6:462.
  4. Davy, A. and P. Soriano (2005) Dev. Dyn. 232:1.
  5. Bohme, B et al. (1993) Oncogene 8:2857.
  6. Pandey, A. et al. (1995) Science 268:567.
  7. Bartley, T.D. et al. (1994) Nature 368:558.
  8. Pandey, A. et al. (1994) J. Biol. Chem. 269:30154.
  9. Miao, H. et al. (2001) Nat. Cell Biol. 3:527.
  10. Orsulic, S. and R. Kemler (2000) J. Cell Sci. 113:1793.
  11. Dohn, M. et al. (2001) Oncogene 20:6503.
  12. Zelinski, D.P. et al. (2001) Cancer Res. 61:2301.
  13. Brantley, D.M. et al. (2002) Oncogene 21:7011.
  14. Ogawa, K. et al. (2000) Oncogene 19:6043.
  15. Sulman, E.P. et al. (1997) Genomics 40:371.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EphA2 Antibody (AF3035)(20)

We have publications tested in 2 confirmed species: Human, Primate.

We have publications tested in 7 applications: Flow Cytometry, Functional Assay, ICC, IHC, IHC-Fr, IHC-P, Western Blot.


Filter By Application
Flow Cytometry
(1)
Functional Assay
(1)
ICC
(3)
IHC
(1)
IHC-Fr
(1)
IHC-P
(1)
Western Blot
(5)
All Applications
Filter By Species
Human
(10)
Primate
(1)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using AF3035 Applications Species
Lucien Torlot, Anna Jarzab, Johanna Albert, Ágnes Pók-Udvari, Arndt Stahler, Julian Walter Holch et al. Proteomics uncover EPHA2 as a potential novel therapeutic target in colorectal cancer cell lines with acquired cetuximab resistance Journal of Cancer Research and Clinical Oncology 2/1/2023 [PMID: 36401637]
Nadia Trivieri, Alberto Visioli, Gandino Mencarelli, Maria Grazia Cariglia, Laura Marongiu, Riccardo Pracella et al. Growth factor independence underpins a paroxysmal, aggressive Wnt5aHigh/EphA2Low phenotype in glioblastoma stem cells, conducive to experimental combinatorial therapy Journal of Experimental & Clinical Cancer Research 12/1/2022 [PMID: 35414102]
J Jukonen, L Moyano-Gal, K Höpfner, EA Pietilä, L Lehtinen, K Huhtinen, E Gucciardo, J Hynninen, S Hietanen, S Grénman, PM Ojala, O Carpén, K Lehti Aggressive and recurrent ovarian cancers upregulate ephrinA5, a non-canonical effector of EphA2 signaling duality Scientific Reports, 2021-04-23;11(1):8856. 2021-04-23 [PMID: 33893375] (ICC, IHC, Western Blot, Human) ICC, IHC, Western Blot Human
B Prinzing, P Schreiner, M Bell, Y Fan, G Krenciute, S Gottschalk MyD88/CD40 signaling retains CAR T cells in a less differentiated state JCI Insight, 2020-11-05;5(21):. 2020-11-05 [PMID: 33148882] (Western Blot, Human) Western Blot Human
L Moyano-Gal, EA Pietilä, SP Turunen, S Corvigno, E Hjerpe, D Bulanova, U Joneborg, T Alkasalias, Y Miki, M Yashiro, A Chernenko, J Jukonen, M Singh, H Dahlstrand, JW Carlson, K Lehti Adaptive RSK-EphA2-GPRC5A signaling switch triggers chemotherapy resistance in ovarian cancer EMBO Mol Med, 2020-03-02;0(0):e11177. 2020-03-02 [PMID: 32115889] (ICC, Human) ICC Human
A Visioli, F Giani, N Trivieri, R Pracella, E Miccinilli, MG Cariglia, O Palumbo, A Arleo, F Dezi, M Copetti, L Cajola, S Restelli, V Papa, A Sciuto, TP Latiano, M Carella, D Amadori, G Gallerani, R Ricci, S Alfieri, G Pesole, AL Vescovi, E Binda Stemness underpinning all steps of human colorectal cancer defines the core of effective therapeutic strategies EBioMedicine, 2019-05-02;0(0):. 2019-05-02 [PMID: 31056474] (ICC, Human) ICC Human
MR Xu, PF Wei, MZ Suo, Y Hu, W Ding, L Su, YD Zhu, WJ Song, GH Tang, M Zhang, P Li Brucine Suppresses Vasculogenic Mimicry in Human Triple-Negative Breast Cancer Cell Line MDA-MB-231 Biomed Res Int, 2019-01-06;2019(0):6543230. 2019-01-06 [PMID: 30723742] (Western Blot, Human) Western Blot Human
Allison Alwan TerBush, Florianne Hafkamp, Hee Jun Lee, Laurent Coscoy A Kaposi's Sarcoma-Associated Herpesvirus Infection Mechanism Is Independent of Integrins alpha 3 beta 1, alpha V beta 3, and alpha V beta 5 Journal of Virology 9/1/2018 [PMID: 29899108]
Nihal Kaplan, Rosa Ventrella, Han Peng, Sonali Pal-Ghosh, Constadina Arvanitis, Joshua Z. Rappoport et al. EphA2/Ephrin-A1 Mediate Corneal Epithelial Cell Compartmentalization via ADAM10 Regulation of EGFR Signaling Investigative Opthalmology & Visual Science 1/20/2018 [PMID: 29351356]
E Reátegui, KE van der Vo, CP Lai, M Zeinali, NA Atai, B Aldikacti, FP Floyd, A H Khankhel, V Thapar, FH Hochberg, LV Sequist, BV Nahed, B S Carter, M Toner, L Balaj, D T Ting, XO Breakefiel, SL Stott Engineered nanointerfaces for microfluidic isolation and molecular profiling of tumor-specific extracellular vesicles Nat Commun, 2018-01-12;9(1):175. 2018-01-12 [PMID: 29330365] (Functional Assay, Human) Functional Assay Human
Show All 20 Publications.

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Bioinformatics

Gene Symbol EPHA2
Uniprot