Recombinant Rat EphB1 Fc Chimera Protein, CF

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Product Details

Summary
Reactivity RtSpecies Glossary
Applications Binding Activity
Format
Carrier-Free

Order Details

Recombinant Rat EphB1 Fc Chimera Protein, CF Summary

Details of Functionality
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat EphB1 Fc Chimera at 2 µg/mL (100 µL/well) can bind recombinant mouse Ephrin‑B1 Fc Chimera with a linear range of 0.31-20 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived rat EphB1 protein
Rat EphB1
(Met18-Gln538)
Accession # P09759
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Met18
Structure / Form
Disulfide-linked homodimer
Protein/Peptide Type
Recombinant Proteins
Gene
Ephb1
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
85 kDa (monomer).
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
102 kDa, reducing conditions
Publications
Read Publications using
1596-B1 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris.
Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 200 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Rat EphB1 Fc Chimera Protein, CF

  • Cek6
  • EC 2.7.10
  • EC 2.7.10.1
  • EK6
  • Elk
  • EPH receptor B1
  • eph tyrosine kinase 2
  • EphB1
  • EPH-like kinase 6
  • ephrin type-B receptor 1
  • EPHT2FLJ37986
  • Hek6
  • Net
  • NETHek6
  • soluble EPHB1 variant 1
  • Tyrosine-protein kinase receptor EPH-2

Background

EphB1, also known as Elk, Cek6, Net, and Hek6 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and the cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphB1 has been shown to bind ephrin-B2, ephrin-B1,
ephrin‑A3, ephrin-A1, ephrin-A4, and ephrin-B3 (2, 3). The extracellular domains of human and rat EphB1 share 99% amino acid identity. Only membrane-bound or
Fc‑clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The ephrin/Eph families also appear to play a role in angiogenesis (3).

  1. Eph Nomenclature Committee [letter]. (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.

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1596-B1
Species: Rt
Applications: Binding Activity

Publications for EphB1 (1596-B1)(7)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 3 applications: Bioassay, EnzAct, In Vivo.


Filter By Application
Bioassay
(3)
EnzAct
(1)
In Vivo
(2)
All Applications
Filter By Species
Human
(3)
Mouse
(1)
Rat
(2)
All Species
Showing Publications 1 - 7 of 7.
Publications using 1596-B1 Applications Species
GE Tyzack, CE Hall, CR Sibley, T Cymes, S Forostyak, G Carlino, IF Meyer, G Schiavo, SC Zhang, GM Gibbons, J Newcombe, R Patani, A Lakatos A neuroprotective astrocyte state is induced by neuronal signal EphB1 but fails in ALS models Nat Commun, 2017;8(1):1164. 2017 [PMID: 29079839]
Hyung-Seok Kim Proteomic Analysis of a Rat Cerebral Ischemic Injury Model after Human Cerebral Endothelial Cell Transplantation J Korean Neurosurg Soc, 2016;59(6):544-550. 2016 [PMID: 27847565] (Bioassay, Human) Bioassay Human
Zhang, Gu, Brady, John, Liang, Wei-Chin, Wu, Yan, Henkemeyer, Mark, Yan, Minhong EphB4 forward signalling regulates lymphatic valve development. Nat Commun, 2015;6(0):6625. 2015 [PMID: 25865237] (EnzAct, Human) EnzAct Human
Yu L, Zhou X, Yu J, Huang H, Jiang L, Zhang F, Cao J, Yan M PI3K contributed to modulation of spinal nociceptive information related to ephrinBs/EphBs. PLoS ONE, 2012;7(8):e40930. 2012 [PMID: 22879882] (In Vivo, Mouse) In Vivo Mouse
Slack S, Battaglia A, Cibert-Goton V, Gavazzi I EphrinB2 induces tyrosine phosphorylation of NR2B via Src-family kinases during inflammatory hyperalgesia. Neuroscience, 2008;156(1):175-83. 2008 [PMID: 18694808] (In Vivo, Rat) In Vivo Rat
Parker M, Roberts R, Enriquez M, Zhao X, Takahashi T, Pat Cerretti D, Daniel T, Chen J Reverse endocytosis of transmembrane ephrin-B ligands via a clathrin-mediated pathway. Biochem. Biophys. Res. Commun., 2004;323(1):17-23. 2004 [PMID: 15351694] (Bioassay, Human) Bioassay Human
Dobrzanski P, Hunter K, Jones-Bolin S, Chang H, Robinson C, Pritchard S, Zhao H, Ruggeri B Antiangiogenic and antitumor efficacy of EphA2 receptor antagonist. Cancer Res., 2004;64(3):910-9. 2004 [PMID: 14871820] (Bioassay, Rat) Bioassay Rat

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Bioinformatics

Gene Symbol Ephb1
Entrez
Uniprot