Endoglin/CD105 Antibody (MJ7/18) [DyLight 550]

Immunohistochemistry-Frozen: Endoglin/CD105 Antibody (MJ7/18) [DyLight 550] [NB100-77666R] - Staining of CD105 (endoglin) in 4T1 tumors after radioimmunotherapy shows CD105 expression (red), blood vessels (green), and nuclei (blue).

• Reactivity: Mu
• Applications: WB, Flow, ICC/IF, IHC, IP
• Clone: MJ7/18
• Clonality: Monoclonal
• Host: Rat
• Conjugate: DyLight 550

Endoglin/CD105 Antibody (MJ7/18) [DyLight 550] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: Inflamed mouse skin
• Epitope: Endothelial cell selective TGB1 binding molecule-endoglin
• Marker: Neo-endothelial Cells Marker
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Rat
• Gene: ENG
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Flow (Cell Surface)
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product NB100-77666. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein G purified

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for Endoglin/CD105 Antibody (MJ7/18) [DyLight 550]

• CD105 antigen
• CD105
• Endoglin
• ENDOsler-Rendu-Weber syndrome 1
• ENG
• HHT1FLJ41744
• ORW
• ORW1

Background

CD105 is a 90 kD homodimeric type I integral membrane glycoprotein, also known as endoglin. It is expressed on endothelial cells (especially on angiogenic endothelial cells) and up-regulated by hypoxia, activated monocytes, macrophages, bone marrow stromal cells, and some cytotrophoblasts. CD105 is a receptor for TGF- szlig1, TGF- szlig3 and modulates TGF- szlig signaling by interacting with TGF- szlig receptors I and/or II. CD105 also binds other growth factors such as actvin A, BMP-2 and -7. CD105 has been show to be a useful marker for identifying proliferating endothelium involved in tumor angiogenesis and can be used for tumor imaging, prognosis, as well as has therapeutic potential for some solid tumors and other angiogenic diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Endoglin/CD105 Antibody (NB100-77666R)(1)

Publication using NB100-77666R

• Ehlerding E, Lacognata S, Jiang D et al. Targeting angiogenesis for radioimmunotherapy with a 177Lu-labeled antibody. Eur J Nucl Med Mol Imaging 2017-08-18 [PMID: 28821931] (ICC/IF, Mouse)

Review for Endoglin/CD105 Antibody (NB100-77666R) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: 4T1 Tumor and Mouse.

reviewed by: Christopher England IHC-Fr 4T1 Tumor and Mouse 02/23/2017

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: 4T1 tumor tissue
• Species: 4T1 Tumor and Mouse

Comments

• Comments: Antibody was used at a concentration of 1:200

FAQs for Endoglin/CD105 Antibody (NB100-77666R). (Showing 1 - 2 of 2 FAQ).

1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
   • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
2. We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
   • We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Bioinformatics

• Gene Symbol: ENG