Endoglin/CD105 Antibody (MJ7/18) [DyLight 550]

Images

 
Immunohistochemistry-Frozen: Endoglin/CD105 Antibody (MJ7/18) [DyLight 550] [NB100-77666R] - Staining of CD105 (endoglin) in 4T1 tumors after radioimmunotherapy shows CD105 expression (red), blood vessels (green), and ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC, IHC-Fr
Clone
MJ7/18
Clonality
Monoclonal
Host
Rat
Conjugate
DyLight 550

Endoglin/CD105 Antibody (MJ7/18) [DyLight 550] Summary

Immunogen
Inflamed mouse skin
Epitope
Endothelial cell selective TGB1 binding molecule-endoglin
Marker
Neo-endothelial Cells Marker
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
ENG
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
Application Notes
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume. Immunohistochemistry-frozen section. It is recommended that the reagent be titrated for optimal performance for each application. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 28821931).
Reviewed Applications
Read 1 Review rated 4
using
NB100-77666R in the following applications:

Publications
Read Publication using
NB100-77666R in the following applications:

Reactivity Notes

Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Protein G purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Endoglin/CD105 Antibody (MJ7/18) [DyLight 550]

  • CD105 antigen
  • CD105
  • Endoglin
  • ENDOsler-Rendu-Weber syndrome 1
  • ENG
  • HHT1FLJ41744
  • ORW
  • ORW1

Background

CD105 is a 90 kD homodimeric type I integral membrane glycoprotein, also known as endoglin. It is expressed on endothelial cells (especially on angiogenic endothelial cells) and up-regulated by hypoxia, activated monocytes, macrophages, bone marrow stromal cells, and some cytotrophoblasts. CD105 is a receptor for TGF- szlig1, TGF- szlig3 and modulates TGF- szlig signaling by interacting with TGF- szlig receptors I and/or II. CD105 also binds other growth factors such as actvin A, BMP-2 and -7. CD105 has been show to be a useful marker for identifying proliferating endothelium involved in tumor angiogenesis and can be used for tumor imaging, prognosis, as well as has therapeutic potential for some solid tumors and other angiogenic diseases.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Endoglin/CD105 Antibody (NB100-77666R)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
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Mouse
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Review for Endoglin/CD105 Antibody (NB100-77666R) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: 4T1 Tumor and Mouse.
We have 1 review tested in 1 application: IHC-Fr.

Reviews using NB100-77666R:
Filter by Applications
IHC-Fr
(1)
All Applications
Filter by Species
4T1 Tumor and Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen Endoglin/CD105 NB100-77666R
Enlarge
reviewed by:
Christopher England
IHC-Fr 4T1 Tumor and Mouse 02/23/2017
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample Tested4T1 tumor tissue
Species4T1 Tumor and Mouse

Comments

CommentsAntibody was used at a concentration of 1:200

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Endoglin/CD105 Antibody (NB100-77666R). (Showing 1 - 2 of 2 FAQ).

  1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
    • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
  2. We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Allophycocyanin Labeled NB100-77666APC
DyLight 350 Labeled NB100-77666UV
DyLight 405 Labeled NB100-77666V
DyLight 488 Labeled NB100-77666G
DyLight 650 Labeled NB100-77666C
DyLight 680 Labeled NB100-77666FR
DyLight 755 Labeled NB100-77666IR
PE Labeled NB100-77666PE
PerCP Labeled NB100-77666PCP

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Bioinformatics Tool for Endoglin/CD105 Antibody (NB100-77666R)

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Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Christopher England
02/23/2017
Application: IHC-Fr
Species: 4T1 Tumor and Mouse

Bioinformatics

Gene Symbol ENG
Entrez