ALCAM/CD166 Antibody [Unconjugated]

Images

 
Western blot shows lysates of mouse brain (cerebellumn) tissue, rat brain (cerebellum) tissue, H4‑II‑E‑C3 rat hepatoma cell line, and U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane ...read more
Mouse splenocytes either (A) activated or (B) resting were stained with Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1172) followed by Phycoerythrin-conjugated ...read more
Canine peripheral blood mononuclear cells (PBMCs) treated with PMA and Calcium Ionomycin for 24 hours were stained with Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
ALCAM/CD166 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1172) at 1.7 µg/mL overnight at 4 °C. ...read more
ALCAM/CD166 was detected in immersion fixed undifferentiated rat mesenchymal stem cells using Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1172) at 10 µg/mL for 3 ...read more
Simple Western lane view shows lysates of mouse brain tissue and mouse lung tissue loaded at 0.2 mg/mL. A specific band was detected for ALCAM/CD166 at approximately 149 kDa (as indicated) using 12.5 µg/mL of ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Simple Western, Flow, IHC, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

ALCAM/CD166 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ALCAM/CD166
Trp28-Lys527
Accession # AAC06342
Specificity
Detects human, mouse and rat ALCAM/CD166 in Western blots. In direct ELISAs, less than 10% cross-reactivity with rhBCAM, recombinant mouse (rm) OCAM, and rmMAdCAM‑1 is observed. Detects canine ALCAM/CD166 in flow cytometry.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ALCAM
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 5-15 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 12.5 ug/mL
  • Western Blot 0.2 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF1172 in the following applications:

Publications
Read Publications using
AF1172 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ALCAM/CD166 Antibody [Unconjugated]

  • activated leucocyte cell adhesion molecule
  • activated leukocyte cell adhesion moleculeFLJ38514
  • ALCAM
  • CD166 antigen
  • CD166
  • MEMDMGC71733

Background

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain, and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation (1-4).  The ECD of mouse ALCAM shares 97.5% and 93.2% aa sequence identity with rat and canine ALCAM ECD, respectively.

  1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
  2. Aruffo, A. et al. (1997) Immunol. Today 18:498.
  3. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
  4. Van Kempen, L. et al. (2001) J. Biol. Chem. 276:25783.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ALCAM/CD166 Antibody (AF1172)(21)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 8 applications: Flow Cytometry, ICC, IF, IHC, IHC-Fr, IHC-P, Immunoprecipitation, Western Blot.


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Flow Cytometry
(3)
ICC
(3)
IF
(1)
IHC
(8)
IHC-Fr
(1)
IHC-P
(1)
Immunoprecipitation
(1)
Western Blot
(1)
All Applications
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Human
(4)
Mouse
(12)
Rat
(1)
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Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using AF1172 Applications Species
Mapunda JA, Pareja J, Vladymyrov M et al. VE-cadherin in arachnoid and pia mater cells serves as a suitable landmark for in vivo imaging of CNS immune surveillance and inflammation Nat Commun 2023-09-20 [PMID: 37730744]
S He, S Li, J Guo, X Zeng, D Liang, Y Zhu, Y Li, D Yang, X Zhao CD166-specific CAR-T cells potently target colorectal cancer cells Translational Oncology, 2022-10-31;27(0):101575. 2022-10-31 [PMID: 36327697] (Flow Cytometry, ICC, Western Blot, Human) Flow Cytometry, ICC, Western Blot Human
Camila Delgado, Lei Bu, Jie Zhang, Fang-Yu Liu, Joseph Sall, Feng-Xia Liang et al. Neural cell adhesion molecule is required for ventricular conduction system development Development 2021-06-01 [PMID: 34100064]
Hiromi Yamashita, Keiichi Fukuda, and Fumiyuki Hattori Hepatocyte-like Cells Derived from Human Pluripotent Stem Cells Can Be Enriched by a Combination of Mitochondrial Content and Activated Leukocyte Cell Adhesion Molecule Japan Medical Association Journal 2019-09-04 [PMID: 33615028] (IHC, Flow Cytometry, Human, Mouse) IHC, Flow Cytometry Human, Mouse
AHM Ng, P Khoshakhla, JE Rojo Arias, G Pasquini, K Wang, A Swiersy, SL Shipman, E Appleton, K Kiaee, RE Kohman, A Vernet, M Dysart, K Leeper, W Saylor, JY Huang, A Graveline, J Taipale, DE Hill, M Vidal, JM Melero-Mar, V Busskamp, GM Church A comprehensive library of human transcription factors for cell fate engineering Nature Biotechnology, 2020-11-30;0(0):. 2020-11-30 [PMID: 33257861] (ICC, Human) ICC Human
HF Renard, F Tyckaert, C Lo Giudice, T Hirsch, CA Valades-Cr, C Lemaigre, M Shafaq-Zad, C Wunder, R Wattiez, L Johannes, P van der Br, D Alsteens, P Morsomme Endophilin-A3 and Galectin-8 control the clathrin-independent endocytosis of CD166 Nat Commun, 2020-03-19;11(1):1457. 2020-03-19 [PMID: 32193381] (IF, Mouse) IF Mouse
CR Bye, V Rytova, WF Alsanie, CL Parish, LH Thompson Axonal growth of midbrain dopamine neurons is modulated by the cell adhesion molecule ALCAM through trans-heterophilic interaction with L1Cam, ChL1 and Semaphorin J. Neurosci., 2019-07-12;0(0):. 2019-07-12 [PMID: 31300520] (IHC, Mouse) IHC Mouse
Ann-Helen Willrodt, Ann-Charlott Salabarria, Philipp Schineis, Desislava Ignatova, Morgan Campbell Hunter, Martina Vranova et al. ALCAM Mediates DC Migration Through Afferent Lymphatics and Promotes Allospecific Immune Reactions Frontiers in Immunology 2019-04-12 [PMID: 31031759]
Ubadah Sabbagh, Aboozar Monavarfeshani, Kaiwen Su, Masoud Zabet‐Moghadam, James Cole, Eric Carnival et al. Distribution and development of molecularly distinct perineuronal nets in visual thalamus Journal of Neurochemistry 2018-12-01 [PMID: 30326149]
J Liu, JDS Reggiani, MA Laboulaye, S Pandey, B Chen, JLR Rubenstein, A Krishnaswa, JR Sanes Tbr1 instructs laminar patterning of retinal ganglion cell dendrites Nat. Neurosci., 2018-04-09;0(0):. 2018-04-09 [PMID: 29632360] (IHC, Mouse) IHC Mouse
Show All 21 Publications.

Reviews for ALCAM/CD166 Antibody (AF1172) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF1172:
Filter by Applications
IF
(2)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
  5
reviewed by:
Verified Customer
IF Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23169771
SpeciesMouse
  4
reviewed by:
Verified Customer
IF Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22243278
SpeciesMouse

Product General Protocols

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WB Video Protocol

FAQs for ALCAM/CD166 Antibody (AF1172) (0)

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mFluor Violet Conjugated Antibodies

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Recent Reviews

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Verified Customer
01/05/2015
Application: IF
Species: Mouse

 
Verified Customer
01/05/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol ALCAM
Uniprot