ALCAM/CD166 Antibody [Unconjugated]

Images

 
Simple Western lane view shows lysates of mouse brain tissue and mouse lung tissue loaded at 0.2 mg/mL. A specific band was detected for ALCAM/CD166 at approximately 149 kDa (as indicated) using 12.5 µg/mL of ...read more
Canine peripheral blood mononuclear cells (PBMCs) treated with PMA and Calcium Ionomycin for 24 hours were stained with Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # ...read more
ALCAM/CD166 was detected in perfusion fixed frozen sections of mouse liver using Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1172) at 1.7 µg/mL overnight at 4 °C. ...read more
ALCAM/CD166 was detected in immersion fixed undifferentiated rat mesenchymal stem cells using Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1172) at 10 µg/mL for 3 ...read more
Western blot shows lysates of mouse brain (cerebellumn) tissue, rat brain (cerebellum) tissue, H4‑II‑E‑C3 rat hepatoma cell line, and U‑118‑MG human glioblastoma/astrocytoma cell line. PVDF membrane ...read more
Mouse splenocytes either (A) activated or (B) resting were stained with Goat Anti-Mouse/Rat/Canine ALCAM/CD166 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1172) followed by Phycoerythrin-conjugated ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, CaSpecies Glossary
Applications WB, Simple Western, Flow, IHC, CyTOF-ready, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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ALCAM/CD166 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse ALCAM/CD166
Trp28-Lys527
Accession # AAC06342
Specificity
Detects human, mouse and rat ALCAM/CD166 in Western blots. In direct ELISAs, less than 10% cross-reactivity with rhBCAM, recombinant mouse (rm) OCAM, and rmMAdCAM‑1 is observed. Detects canine ALCAM/CD166 in flow cytometry.
Details of Functionality
ActivityAssaywCitation not found None None None None None
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ALCAM
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
  • Simple Western 12.5 ug/mL
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 5-15 ug/mL
  • CyTOF-ready
  • Immunocytochemistry 5-15 ug/mL
Reviewed Applications
Read 2 Reviews rated 4.5
using
AF1172 in the following applications:

Publications
Read Publications using
AF1172 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ALCAM/CD166 Antibody [Unconjugated]

  • activated leucocyte cell adhesion molecule
  • activated leukocyte cell adhesion moleculeFLJ38514
  • ALCAM
  • CD166 antigen
  • CD166
  • MEMDMGC71733

Background

ALCAM, activated leukocyte cell adhesion molecule, is a type I membrane glycoprotein and a member of the immunoglobulin supergene family. It is also known as CD166, MEMD, SC-1/DM-GRASP/BEN in the chicken, and KG-CAM in the rat. ALCAM is expressed on thymic epithelial cells, activated B and T cells, and monocytes. ALCAM can bind itself homotypically and is also capable of binding CD6, NgCAM, and other, as of yet, unidentified brain proteins. ALCAM/CD6 interaction may be involved in T cell development and T cell regulation. Additionally, ALCAM/CD6 and ALCAM/NgCAM interactions may play roles in the nervous system. ALCAM has also been observed to be upregulated on highly metastasizing melanoma cell lines and may play a role in tumor migration. ALCAM is a 583 amino acid (aa) protein consisting of a 27 aa signal peptide, a 500 aa extracellular domain, a 24 aa transmembrane domain, and a 32 aa cytoplasmic domain. The extracellular domain of ALCAM contains 5 Ig-like domains of which the amino-terminal V1 domain is essential for ligand binding and ALCAM-mediated cell aggregation (1-4).  The ECD of mouse ALCAM shares 97.5% and 93.2% aa sequence identity with rat and canine ALCAM ECD, respectively.

  1. Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213.
  2. Aruffo, A. et al. (1997) Immunol. Today 18:498.
  3. Degen, W.G. et al. (1998) Am. J. Pathol. 152:805.
  4. Van Kempen, L. et al. (2001) J. Biol. Chem. 276:25783.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ALCAM/CD166 Antibody (AF1172)(10)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC, IHC, IHC-Fr, IHC-P, Immunoprecipitation.


Filter By Application
ICC
(1)
IHC
(6)
IHC-Fr
(1)
IHC-P
(1)
Immunoprecipitation
(1)
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Human
(1)
Mouse
(9)
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Showing Publications 1 - 10 of 10.
Publications using AF1172 Applications Species
J Liu, JDS Reggiani, MA Laboulaye, S Pandey, B Chen, JLR Rubenstein, A Krishnaswa, JR Sanes Tbr1 instructs laminar patterning of retinal ganglion cell dendrites Nat. Neurosci., 2018;0(0):. 2018 [PMID: 29632360] (IHC, Mouse) IHC Mouse
DL Coutu, KD Kokkaliari, L Kunz, T Schroeder Multicolor quantitative confocal imaging cytometry Nat. Methods, 2018;15(1):39-46. 2018 [PMID: 29320487] (IHC, Mouse) IHC Mouse
MA L‚cuyer, O Saint-Laur, L BourbonniŠ, S Larouche, C Larochelle, L Michel, M Charabati, M Abadier, S Zandee, N Haghayegh, E Gowing, C Pittet, R Lyck, B Engelhardt, A Prat Dual role of ALCAM in neuroinflammation and blood-brain barrier homeostasis Proc. Natl. Acad. Sci. U.S.A, 2017;114(4):E524-E533. 2017 [PMID: 28069965] (Immunoprecipitation, Mouse) Immunoprecipitation Mouse
Gu, Zirong, Imai, Fumiyasu, Kim, In Jung, Fujita, Hiroko, Katayama, Kei ichi, Mori, Kensaku, Yoshihara, Yoshihir, Yoshida, Yutaka Expression of the immunoglobulin superfamily cell adhesion molecules in the developing spinal cord and dorsal root ganglion. PLoS ONE, 2015;10(3):e0121550. 2015 [PMID: 25826454] (IHC, Mouse) IHC Mouse
Bye C, Jonsson M, Bjorklund A, Parish C, Thompson L Transcriptome analysis reveals transmembrane targets on transplantable midbrain dopamine progenitors. Proc Natl Acad Sci U S A, 2015;112(15):E1946-55. 2015 [PMID: 25775569] (IHC, Mouse) IHC Mouse
Leibowitz B, Wei L, Zhang L, Ping X, Epperly M, Greenberger J, Cheng T, Yu J Ionizing irradiation induces acute haematopoietic syndrome and gastrointestinal syndrome independently in mice. Nat Commun, 2014;5(0):3494. 2014 [PMID: 24637717] (IHC, Mouse) IHC Mouse
Inamata, Yasuyuki, Shirasaki, Ryuichi Dbx1 triggers crucial molecular programs required for midline crossing by midbrain commissural axons. Development, 2014;141(6):1260-71. 2014 [PMID: 24553291] (IHC, Mouse) IHC Mouse
Yao H, Kim K, Duan M, Hayashi T, Guo M, Morgello S, Prat A, Wang J, Su TP, Buch S Cocaine hijacks sigma1 receptor to initiate induction of activated leukocyte cell adhesion molecule: implication for increased monocyte adhesion and migration in the CNS. J. Neurosci., 2011;31(16):5942-55. 2011 [PMID: 21508219] (IHC-P, Human) IHC-P Human
Di Meglio T, Nguyen-Ba-Charvet KT, Tessier-Lavigne M, Sotelo C, Chedotal A Molecular mechanisms controlling midline crossing by precerebellar neurons. J. Neurosci., 2008;28(25):6285-94. 2008 [PMID: 18562598] (IHC-Fr, Mouse) IHC-Fr Mouse
Stanton LA, Beier F Inhibition of p38 MAPK signaling in chondrocyte cultures results in enhanced osteogenic differentiation of perichondral cells. Exp. Cell Res., 2007;313(1):146-55. 2007 [PMID: 17067574] (ICC, Mouse) ICC Mouse

Reviews for ALCAM/CD166 Antibody (AF1172) (2) 4.52

Average Rating: 4.5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF1172:
Filter by Applications
IF
(2)
All Applications
Filter by Species
Mouse
(2)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IF Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 23169771
SpeciesMouse

Blocking

Blocking DetailsSee PMID 23169771

Details

Detection NotesSee PMID 23169771

Comments

CommentsPublished See PMID 23169771
 
reviewed by:
Anonymous
IF Mouse 01/05/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22243278
SpeciesMouse

Blocking

Blocking DetailsSee PMID 22243278

Details

Detection NotesSee PMID 22243278

Comments

Comments"Binds to ALCAM on neuronal membranes.The antibody also works well in western blot on protein lysates from mouse brain or primary spinal cord cultures."PMID 22243278

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Recent Reviews

4.5
5
1
4
1
3
0
2
0
1
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Anonymous
01/05/2015
Application: IF
Species: Mouse

 
Anonymous
01/05/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol ALCAM
Uniprot