Endoglin/CD105 Antibody (MJ7/18)

Images

 
Western Blot: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Total protein from mouse placenta was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocking in 5% non-fat milk in TBST. The membrane ...read more
Immunocytochemistry/ Immunofluorescence: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Using the DyLight 550 direct conjugate Staining of CD105 (endoglin) in 4T1 tumors after radioimmunotherapy shows CD105 expression ...read more
Flow Cytometry: Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - Analysis of Biotin conjugate of NB100-77666. Surface staining of bEND.3 cell line with 0.25 ug of Anti-Mouse CD105 (Endoglin) Biotin followed by ...read more
Flow (Cell Surface): Endoglin/CD105 Antibody (MJ7/18) [NB100-77666] - A surface stain was performed on MS-1 Cells with Endoglin/CD105 (MJ7/18) antibody NB100-77666 (blue) and a matched isotype control (orange). Cells ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, Flow, Flow-CS, ICC/IF, IHC, IHC-Fr, IP
Clone
MJ7/18
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Endoglin/CD105 Antibody (MJ7/18) Summary

Immunogen
Inflamed mouse skin
Epitope
Endothelial cell selective TGB1 binding molecule-endoglin
Marker
Neo-endothelial Cells Marker
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Rat
Gene
ENG
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:2000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Flow (Cell Surface)
Application Notes
For immunofluorescent staining, the suggested use of this reagent is: 0.5 microgram per one million cells in 100 microliter volume. Immunohistochemistry-frozen section. It is recommended that the reagent be titrated for optimal performance for each application.

Reactivity Notes

Mouse.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Tris-Glycine
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Endoglin/CD105 Antibody (MJ7/18)

  • CD105 antigen
  • CD105
  • Endoglin
  • ENDOsler-Rendu-Weber syndrome 1
  • ENG
  • HHT1FLJ41744
  • ORW
  • ORW1

Background

CD105, commonly referred to as Endoglin, ENG, HHT1, and ORW1 is a membrane single-pass type I membrane protein limited to endothelial cells in all tissues except bone marrow. It is a major glycoprotein of vascular endothelium and plays a major role in the regulation of angiogenesis. CD105 acts as a TGF-beta coreceptor and is involved in the TGF-beta/BMP signaling cascade. Mutations involving CD105 have been shown to be involved with hereditary hemorrhagic telangiectasia, as well interruptions of the TGF-beta/BMP pathways leading to some cancers. Immunohistochemistry has shown that CD105 has a very high expression in blood vessels of tumors, making it a potential target that can be used in tumor imaging (PMID: 12773481). Elevated serum levels of CD105 in pregnant women have been determined a possible risk factor for preeclampsia (PMID: 24273904).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, Flow, Flow-CS, ICC/IF, IHC, IHC-Fr, IP

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Endoglin/CD105 Antibody (NB100-77666). (Showing 1 - 2 of 2 FAQ).

  1. I wonder if you have a CD105 or CD34 antibody suitable for IHC that is specific for human and do not bind mouse?
    • We do not have any anti-human CD34 or CD105 antibodies that are confirmed to NOT detect the mouse protein. When we have tested an antibody and confirmed that it will not react with mouse samples, we will add Mu(-) to the datasheet, and unfortunately all of our CD105 and CD34 antibodies will either detect the mouse protein, or they have not been used in mouse samples before.
  2. We are interested in CD105 antibodies that could react with dog, please let me know which products in your catalog you would recommend.
    • We do not currently carry any CD105 antibodies that have been validated for use in dog samples. However, if you are interested in testing any of our CD105 antibodies with dog samples you would be eligible for our Innovators Reward Program, in which you can get a discount voucher for the purchase price of the product. Please contact us at innovators@novusbio.com with any questions regarding this program.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-77666AF405
Alexa Fluor 488 Labeled NB100-77666AF488
Alexa Fluor 647 Labeled NB100-77666AF647
Alexa Fluor 700 Labeled NB100-77666AF700
Allophycocyanin Labeled NB100-77666APC
DyLight 350 Labeled NB100-77666UV
DyLight 405 Labeled NB100-77666V
DyLight 488 Labeled NB100-77666G
DyLight 550 Labeled NB100-77666R
DyLight 650 Labeled NB100-77666C
DyLight 680 Labeled NB100-77666FR
DyLight 755 Labeled NB100-77666IR
PE Labeled NB100-77666PE
PerCP Labeled NB100-77666PCP

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Bioinformatics

Gene Symbol ENG
Entrez