EDIL3/DEL1 Antibody - BSA Free

Western Blot: EDIL3/DEL1 Antibody [NBP1-28632] - Lane 1: Western blot on normal human brain, Lane 2: Western blot on normal mouse brain.

Immunocytochemistry/Immunofluorescence: EDIL3/DEL1 Antibody [NBP1-28632] - HeLa cells stained NBP1-28632 (Green) detected with DyLight Fluor 488 conjugated anti-rabbit IgG secondary antibody. Nuclei are counterstained with Hoechst 33258 (Blue).

Simple Western: EDIL3/DEL1 Antibody [NBP1-28632] - Simple Western lane view shows a specific band for EDIL3/DEL1 in 0.5 mg/ml of Human Brain lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

EDIL3/DEL1was detected in immersion fixed U-2 OS human osteosarcoma cell line using Rabbit anti-EDIL3/DEL1Antigen Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 647 (Catalog # NBP1-28632AF647) (light blue) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

EDIL3/DEL1was detected in immersion fixed U-2 OS human osteosarcoma cell line using Rabbit anti-EDIL3/DEL1Antigen Affinity Purified Polyclonal Antibody conjugated to Alexa Fluor® 488 (Catalog # NBP1-28632AF488) (green) at 10 µg/mL overnight at 4C. Cells were counterstained with DAPI (blue). Cells were imaged using a 100X objective and digitally deconvolved.

• Reactivity: Hu, Mu
• Applications: WB, Simple Western, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.62 mg/ml

EDIL3/DEL1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit EDIL3/DEL1 Antibody - BSA Free (NBP1-28632) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and Simple Western. Anti-EDIL3/DEL1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic peptide made to an internal portion of human Del-1 (within residues 200-350). [Swiss-Prot# O43854]
• Localization: Secreted.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: EDIL3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:100
  • Simple Western 1:20
  • Western Blot 2 ug/ml
• Application Notes: This Del-1 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot analysis, where a band can be seen at approx. 53 kDa.
  
  In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
  See Simple Western Antibody Database for Simple Western validation: Tested in Human Brain lysate 0.5 mg/mL, separated by Size, antibody dilution of 1:20, apparent MW was 61 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
  The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
  Human Brain Whole Tissue Lysate (Adult Whole Normal)

• Control Peptide:

  EDIL3/DEL1 Peptide (NBP1-28632PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.1% Sodium Azide
• Concentration: 1.62 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for EDIL3/DEL1 Antibody - BSA Free

• DEL1
• DEL1developmental endothelial locus-1
• Developmentally-regulated endothelial cell locus 1 protein
• EDIL3
• EGF-like repeat and discoidin I-like domain-containing protein 3
• EGF-like repeats and discoidin I-like domains 3
• Integrin-binding protein DEL1
• MGC26287

Background

Del-1 is an integrin ligand that is expressed by endothelial cells during embryological vascular development. It promotes adhesion of endothelial cells through an interaction with the alpha-v/beta-3 integrin receptor. Del-1 plays a vital role in mediating angiogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for EDIL3/DEL1 Antibody (NBP1-28632)(3)

Publications using NBP1-28632

• Romanidou G, Konstantinidis TG, Natsi AM et al. Decreased Levels of Soluble Developmental Endothelial Locus-1 Are Associated with Thrombotic Microangiopathy in Pregnancy International journal of molecular sciences 2023-07-21 [PMID: 37511523] (IHC-P, Human)
• Zhu C, Li Z, Li B et al. Unaltered prion disease in mice lacking developmental endothelial locus-1 (Del 1) bioRxiv 2018 Jun 16 [PMID: 30743056]
• Zhu C, Li Z, Li B et al. Unaltered prion disease in mice lacking developmental endothelial locus-1 (Del 1) bioRxiv 2018 Jun 16 [PMID: 30743056]

Bioinformatics

• Gene Symbol: EDIL3
• Entrez:
  • 10085(Human)
• Uniprot:
  • O43854()