Integrin alpha V beta 5 Antibody (P5H9)

Integrin alpha V beta 5 was detected in immersion fixed HT1080 human fibrosarcoma cell line using Human Integrin alpha V beta 5 Monoclonal Antibody (Catalog # MAB2528) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reactivity: Hu
• Applications: Flow, IP, B/N, CyTOF-ready, ICC/IF
• Clone: P5H9
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Integrin alpha V beta 5 Antibody (P5H9) Summary

• Immunogen: HT1080 human fibrosarcoma cell line
• Specificity: Detects human Integrin alpha V beta 5. Recognizes the human Integrin alpha V beta 5 heterodimer and does not recognize the alpha V subunit in association with any other beta subunits.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGAV
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunoprecipitation
  • Adhesion Blockade
  • CyTOF-ready
  • Immunocytochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha V beta 5 Antibody (P5H9)

• CD51
• Integrin alpha V beta 5
• integrin subunit alpha V
• ITGAV
• MSK8
• VNRA
• VTNR

Background

Integrins are heterodimeric receptors comprised of an alpha and a beta subunit. Integrin alpha V (CD51) associates with several different beta subunits, but Integrin beta 5 associates exclusively with the alpha V subunit. Integrin alpha V beta 5, also known as Integrin alpha V beta S and Integrin alpha V beta 3B, is a transmembrane heterodimeric protein that functions as a receptor for Vitronectin. It is expressed on hepatoma cells, fibroblasts and carcinoma cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha V beta 5 Antibody (MAB2528)(11)

Publications using MAB2528

• M Garcia-Mou, N Martinez-V, M Gonzalez-H, L Marrodán, M Cascallo, R Alemany, A Patiño-Gar, MM Alonso The oncolytic adenovirus VCN-01 promotes anti-tumor effect in primitive neuroectodermal tumor models Sci Rep, 2019;9(1):14368. 2019 [PMID: 31591461] (Flow Cytometry, Human)
• E Peterman, P Gibieža, J Schafer, VA Skeberdis, A Kaupinis, M Valius, X Heiligenst, I Hurbain, G Raposo, R Prekeris The post-abscission midbody is an intracellular signaling organelle that regulates cell proliferation Nat Commun, 2019;10(1):3181. 2019 [PMID: 31320617] (ICC, Human)
• H Huang, Y Dong, Y Zhang, D Ru, Z Wu, J Zhang, M Shen, Y Duan, Y Sun GSH-sensitive Pt(IV) prodrug-loaded phase-transitional nanoparticles with a hybrid lipid-polymer shell for precise theranostics against ovarian cancer Theranostics, 2019;9(4):1047-1065. 2019 [PMID: 30867815] (Flow Cytometry, Human)
• J Wei, A Marisetty, B Schrand, K Gabrusiewi, Y Hashimoto, M Ott, Z Grami, LY Kong, X Ling, HG Caruso, S Zhou, YA Wang, GN Fuller, JT Huse, E Gilboa, N Kang, X Huang, R Verhaak, S Li, AB Heimberger Osteopontin mediates glioblastoma-associated macrophage infiltration and is a therapeutic target J. Clin. Invest., 2018;0(0):. 2018 [PMID: 30307407] (Neutralization, Human)
• K Miyano, H Cabral, Y Miura, Y Matsumoto, Y Mochida, H Kinoh, K Iwata, O Nagano, H Saya, N Nishiyama, K Kataoka, T Yamasoba cRGD peptide installation on cisplatin-loaded nanomedicines enhances efficacy against locally advanced head and neck squamous cell carcinoma bearing cancer stem-like cells J Control Release, 2017;0(0):. 2017 [PMID: 28666729] (Flow Cytometry, Human)
• ME Burgett, JD Lathia, P Roth, AS Nowacki, DS Galileo, E Pugacheva, P Huang, A Vasanji, M Li, T Byzova, T Mikkelsen, S Bao, JN Rich, M Weller, CL Gladson Direct contact with perivascular tumor cells enhances integrin �v?3 signaling and migration of endothelial cells Oncotarget, 2016;7(28):43852-43867. 2016 [PMID: 27270311] (Neutralization, Human)
• Shen Y, Yang F, Wang H, Cai Z, Xu Y, Zhao A, Su Y, Zhang G, Zhu S Arg-Gly-Asp (RGD)-Modified E1A/E1B Double Mutant Adenovirus Enhances Antitumor Activity in Prostate Cancer Cells In Vitro and in Mice. PLoS ONE, 2016;11(1):e0147173. 2016 [PMID: 26799485] (Flow Cytometry, Human)
• Dixon K, O'Flynn J, van der Kooij S, van Kooten C Phagocytosis of apoptotic or necrotic cells differentially regulates the transcriptional expression of IL-12 family members in dendritic cells. J Leukoc Biol, 2014;96(2):313-24. 2014 [PMID: 24782489] (Flow Cytometry, Human)
• Mathieu M, Iampietro M, Chuchana P, Guerit D, Djouad F, Noel D, Jorgensen C Involvement of angiopoietin-like 4 in matrix remodeling during chondrogenic differentiation of mesenchymal stem cells. J Biol Chem, 2014;289(12):8402-12. 2014 [PMID: 24505142] (IHC, Human)
• Wang L, Pedroja BS, Meyers EE, Garcia AL, Twining SS, Bernstein AM Degradation of internalized alphaVbeta5 integrin is controlled by uPAR bound uPA: effect on beta1 integrin activity and alpha-SMA stress fiber assembly. PLoS ONE, 2012;7(3):e33915. 2012 [PMID: 22470492] (Neutralization, Human)
• Cabezon R, Sintes J, Llinas L, Benitez-Ribas D Analysis of HLDA9 mAbs on plasmacytoid dendritic cells. Immunol. Lett., 2011;134(2):167-73. 2011 [PMID: 20933012] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ITGAV