Annexin V Apoptosis Kit [FITC] [NBP2-29373] - Annexin V Apoptosis Kit [NBP2-29373] - Adherent RAW cells were treated with 0.05 ug/ml actinomycin-D for 17 hours to induce apoptosis. Cells were detached with Accutase and ...read more
Annexin V Apoptosis Kit [FITC] [NBP2-29373]
Annexin V Apoptosis Kit [FITC] [NBP2-29373] - During early apoptosis, the plasma membrane loses asymmetry causing PS to be translocated from the cytoplasmic face of the plasma membrane to the external face which can be ...read more
Annexin V Apoptosis Kit [FITC] [NBP2-29373] - Control Cells were stained or unstained as indicated using the Annexin V-FITC Apoptosis Detection Kit.
Use in Flow cell surface reported in scientific literature (PMID 23955790) Use the Control Cells provided to set up compensation and quadrants. The Control Cells are positive for both Annexin V-FITC and PI. The basal level of apoptosis and necrosis varies considerably within a give cell population. Even in the absence of induced apoptosis, most cell populations will contain a minor percentage of cells that are positive for apoptosis ((Annexin V-FITC positive, PI negative) and dead, necrotic, or in the late stages of apoptosis (Annexin V-FITC positive, PI positive). Thus an untreated cell population is used to define the basal level of apoptotic and dead cells. Determine the percentage of cells that have been induced to undergo apoptosis by subtracting the percentage of apoptotic cells in the untreated from the treated population. Since cell death is the eventual outcome of cells undergoing apoptosis, cells in the late stages of apoptosis will have a damaged membrane and stain positive for PI as well as for Annexin V-FITC. Consequently cells which have undergone necrosis are not distinguishable from those which have undergone apoptosis. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 25947082). Use in FLOW cytometry reported in scientific literature ( PMID 27448441)
Read Publications using NBP2-29373 in the following applications:
Human reactivity reported in scientific literature (PMID: 23955790) Mouse reactivity reported in scientific literature (PMID: 22566271). Rat reactivity reported in scientific literature (PMID: 25947082)
Packaging, Storage & Formulations
Store at 4C. Do not freeze.
10X Binding Buffer
Control Cells: 1 x 10^6 cells/ml
Propidium Iodide (PI)
ADDITIONAL ITEMS REQUIRED (NOT INCLUDED IN THE KIT) Distilled H2O Flow Cytometer Experimental Cells Centrifuge Positive Control Cell Line (recommended, not required)
Alternate Names for Annexin V Apoptosis Kit [FITC]
Placental anticoagulant protein 4
Placental anticoagulant protein I
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.