Annexin V Apoptosis Kit [FITC]

Images

 
Annexin V Apoptosis Kit [FITC] [NBP2-29373] - Annexin V Apoptosis Kit [NBP2-29373] - Adherent RAW cells were treated with 0.05 ug/ml actinomycin-D for 17 hours to induce apoptosis. Cells were detached with Accutase and ...read more
Annexin V Apoptosis Kit [FITC] [NBP2-29373]
Annexin V Apoptosis Kit [FITC] [NBP2-29373] - During early apoptosis, the plasma membrane loses asymmetry causing PS to be translocated from the cytoplasmic face of the plasma membrane to the external face which can be ...read more
Annexin V Apoptosis Kit [FITC] [NBP2-29373] - Control Cells were stained or unstained as indicated using the Annexin V-FITC Apoptosis Detection Kit.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications Flow, Flow-CS, ICC/IF
Conjugate
FITC

Order Details

Annexin V Apoptosis Kit [FITC] Summary

Kit Type
Apoptosis Kit
Gene
ANXA5

Applications/Dilutions

Application Notes
Use in Flow cell surface reported in scientific literature (PMID 23955790) Use the Control Cells provided to set up compensation and quadrants. The Control Cells are positive for both Annexin V-FITC and PI.
The basal level of apoptosis and necrosis varies considerably within a give cell population. Even in the absence of induced apoptosis, most cell populations will contain a minor percentage of cells that are positive for apoptosis ((Annexin V-FITC positive, PI negative) and dead, necrotic, or in the late stages of apoptosis (Annexin V-FITC positive, PI positive). Thus an untreated cell population is used to define the basal level of apoptotic and dead cells.
Determine the percentage of cells that have been induced to undergo apoptosis by subtracting the percentage of apoptotic cells in the untreated from the treated population. Since cell death is the eventual outcome of cells undergoing apoptosis, cells in the late stages of apoptosis will have a damaged membrane and stain positive for PI as well as for Annexin V-FITC.
Consequently cells which have undergone necrosis are not distinguishable from those which have undergone apoptosis. Use in Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 25947082). Use in FLOW cytometry reported in scientific literature ( PMID 27448441)
Publications
Read Publications using
NBP2-29373 in the following applications:

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 23955790) Mouse reactivity reported in scientific literature (PMID: 22566271). Rat reactivity reported in scientific literature (PMID: 25947082)

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.

Kit Components

Components
  1. Control Cells: 1 x 10^6 cells/ml
  2. Annexin V-FITC
  3. 10X PBS
  4. 10X Binding Buffer
  5. Propidium Iodide (PI)

Notes

ADDITIONAL ITEMS REQUIRED (NOT INCLUDED IN THE KIT)
Distilled H2O
Flow Cytometer
Experimental Cells
Centrifuge
Positive Control Cell Line (recommended, not required)

Alternate Names for Annexin V Apoptosis Kit [FITC]

  • Anchorin CII
  • annexin A5
  • Annexin V
  • annexin-5
  • ANX5
  • ANXA5
  • ANXV
  • Calphobindin I
  • Endonexin II
  • ENX2CBP-I
  • Lipocortin V
  • PAP-I
  • Placental anticoagulant protein 4
  • Placental anticoagulant protein I
  • PP4
  • Thromboplastin inhibitor
  • VAC-alpha
  • Vascular anticoagulant-alpha

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Kits are guaranteed for 6 months from date of receipt.

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Publications for Annexin V Kit (NBP2-29373)(20)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 5 applications: FLOW, Flow, Flow - CS, Flow-CS, ICC/IF.


Filter By Application
FLOW
(8)
Flow
(1)
Flow - CS
(1)
Flow-CS
(5)
ICC/IF
(2)
All Applications
Filter By Species
Human
(14)
Mouse
(2)
Rat
(2)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NBP2-29373 Applications Species
Hartung F, Pardo LA. Guiding TRAIL to cancer cells through Kv10.1 potassium channel overcomes resistance to doxorubicin Eur. Biophys. J. 2016 Jun 27 [PMID: 27350552] (ICC/IF, Human) ICC/IF Human
Narendrula R. rRNA Disruption: A Predictive Marker of Response to Taxane Chemotherapy. Thesis 2014 (Flow-CS, Human)

Details:
A2789 human ovarian carcinoma cell line, Fig 12.
Flow-CS Human
Xue Y, Wu L, Liu Y et al. ENTPD5 Induces Apoptosis in Lung Cancer Cells via Regulating Caspase 3 Expression PLoS ONE 2015 Mar 21 [PMID: 25794010] (FLOW, Human) FLOW Human
Kumar A, Kant S, Singh SM. Targeting monocarboxylate transporter by alpha-cyano-4-hydroxycinnamate modulates apoptosis and cisplatin resistance of Colo205 cells: implication of altered cell survival regulation. Apoptosis 2013 Dec [PMID: 23955790] (Flow-CS, Human) Flow-CS Human
Li FJ, Schreeder DM, Li R et al. FCRL3 promotes TLR9-induced B-cell activation and suppresses plasma cell differentiation. Eur J Immunol 2013 Nov [PMID: 23857366] (FLOW, Human)

Details:
Purified human blood B cells, Fig 2C.
FLOW Human
Vishvakarma NK, Kumar A, Singh V et al. Hyperglycemia of tumor microenvironment modulates stage-dependent tumor progression and multidrug resistance: implication of cell survival regulatory molecules and altered glucose transport. Mol Carcinog 2013 Dec [PMID: 22566271] (Flow-CS, Mouse) Flow-CS Mouse
Saydam O, Senol O, Wurdinger T et al. miRNA-7 attenuation in Schwannoma tumors stimulates growth by upregulating three oncogenic signaling pathways. Cancer Res. 2011 Feb 1 [PMID: 21156648] (Flow - CS, Human)

Details:
Flow (cell surface): Human primary Schwannoma and HEI-193 cell lines, S1.
Flow - CS Human
Badr CE, Niers JM, Morse D et al. Suicidal gene therapy in an NF-kB-controlled tumor environment as monitored by a secreted blood reporter. Gene Ther. 2011 May [PMID: 21150937] (Human)

Details:
Human glioma (Gli36) cells infected with lenti-NF-CU-IGluc and treated with TNF-alpha, 5-FC, or 5FC+TNF alpha, Fig 2D.
Human
Lu L, Wang S, Zheng L et al. Amyotrophic lateral sclerosis-linked mutant SOD1 sequesters Hu antigen R (HuR) and TIA-1-related protein (TIAR): implications for impaired post-transcriptional regulation of vascular endothelial growth factor. J Biol Chem. 2009 Dec 4 [PMID: 19805546]
Skivka LM, Fedorchuk OG, Bezdeneznykh NO. The effect of antineoplastic drug NSC631570 on immunogenicity of B16 melanoma. J Exp Integr Med 2014 Mar 24 (Flow-CS, Mouse)

Details:
Metastatic mlanoma cells, Fig 2.
Flow-CS Mouse
Show All 20 Publications.

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Video Protocols

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Bioinformatics

Gene Symbol ANXA5
Entrez