Recombinant Mouse MFG-E8 Protein


There are currently no images for MFG-E8 (2805-MF).

Every product we sell is backed by Novus' 100% Guarantee. If you have used this product, please submit your images and reviews to earn reward points.

Product Details

Reactivity MuSpecies Glossary
Applications Binding Activity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Mouse MFG-E8 Protein Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED50 for this effect is 10-50 ng/mL.
Mouse myeloma cell line, NS0-derived mouse MFG-E8 protein
Ala23-Cys463, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.


Theoretical MW
49.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
64-72 kDa, reducing conditions
Read Publications using
2805-MF in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse MFG-E8 Protein

  • BA46
  • Breast epithelial antigen BA46
  • EDIL1
  • hP47
  • HsT19888
  • lactadherin
  • Lactahedrin
  • Medin
  • MFG1
  • MFGE8
  • MFG-E8
  • MFGM
  • milk fat globule-EGF factor 8 protein
  • Milk fat globule-EGF factor 8
  • O-acetyl disialoganglioside synthase
  • OAcGD3S
  • SED1
  • SPAG10
  • sperm associated antigen 10
  • sperm surface protein hP47


Milk Fat Globulin Protein E8 (MFG-E8), also known as Lactadherin, MP47, breast epithelial antigen BA46, and SED1, is a 66‑75 kDa pleiotropic secreted glycoprotein that promotes mammary gland morphogenesis, angiogenesis, and tumor progression. MFG-E8 also plays an important role in tissue homeostasis and the prevention of inflammation (1). Mouse MGF-E8 contains two N-terminal EGF-like domains, a Pro/Thr-rich segment, and two C‑terminal F5/8-type discoidin-like domains (2). It MFG-E8 shares 63% and 94% aa sequence identity with comparable regions of human and rat MFG-E8, respectively. Alternative splicing of mouse MFG-E8 generates a short isoform lacking the Pro/Thr-rich region which contains sites for O-linked glycosylation and tyrosine sulfation (3). MFG-E8 is released into the milk in complex with lipid-containing milk fat globules. It is also found in multiple other cell types including endothelial cells and smooth muscle cells of the vasculature, immature dendritic cells, at the acrosomal cap of testicular and epididymal sperm, and in epithelial cells of the endometrium (1). MFG-E8 binds to the Integrins alpha V beta 3 and alpha V beta 5 and potentiates the angiogenic action of VEGF through VEGF R2 (4, 5). It reduces inflammation and tissue damage in a variety of settings. MFG-E8 functions as a bridge between phosphatidylserine on apoptotic cells and Integrin alpha V beta 3 on phagocytes, leading to the clearance of apoptotic debris (6). It mediates the engulfment of apoptotic bodies in atherosclerotic plaques and prion-infected brain (7, 8) and of apoptotic B cells during germinal center reactions (9, 10). MFG-E8 also promotes the removal of excess Collagen in fibrotic lungs and the regeneration of damaged intestinal epithelia (11, 12). Its tissue‑protective role impairs anti‑tumor immunity and chemotherapy‑induced apoptosis (13). MFG-E8 in the breastmilk blocks rotavirus infection in nursing babies (14).
  1. Raymond, A. et al. (2009) J. Cell. Biochem. 106:957.
  2. Stubbs, J.D. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8417.
  3. Hoffhines, A.J. et al. (2008) J. Biol. Chem. 284:3096.
  4. Silvestre, J.-S. et al. (2005) Nat. Med. 11:499.
  5. Borges, E. et al. (2000) J. Biol. Chem. 275:39867.
  6. Hanayama, R. et al. (2002) Nature 417:182.
  7. Ait-Oufella, H. et al. (2007) Circulation 115:2168.
  8. Kranich, J. et al. (2010) J. Exp. Med. 207:2271.
  9. Hanayama, R. et al. (2004) Science 304:1147.
  10. Kranich, J. et al. (2010) J. Exp. Med. 205:1293.
  11. Atabai, K. et al. (2009) J. Clin. Invest. 119:3713.
  12. Bu, H.-F. et al. (2007) J. Clin. Invest. 117:3673.
  13. Jinushi, M. et al. (2009) J. Exp. Med. 206:1317.
  14. Kvistgaard, A.S. et al. (2004) J. Dairy Sci. 87:4088.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Mu
Applications: Flow, IHC, CyTOF-ready, Dual ISH-IHC
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt, Po, Bv, Eq
Applications: WB, ICC/IF, IHC
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P
Species: Hu, Mu, Rt, Ze
Applications: WB, Flow, ICC/IF, IHC, IHC-P, IP
Species: Mu, Rt
Applications: WB, IHC, ELISA(Cap), ELISA(Det), Neut, ELISA(Sta)
Species: Mu
Applications: Binding Activity

Publications for MFG-E8 (2805-MF)(25)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Bioassay, In Vivo, Western Blot.

Filter By Application
In Vivo
Western Blot
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 25. Show All 25 Publications.
Publications using 2805-MF Applications Species
HY Chiang, PH Chu, TH Lee MFG-E8 mediates arterial aging by promoting the proinflammatory phenotype of vascular smooth muscle cells J. Biomed. Sci., 2019;26(1):61. 2019 [PMID: 31470852] (In Vivo, Mouse) In Vivo Mouse
J Li, X Xu, X Cai, Y Weng, Y Wang, Q Shen, X Shi Milk Fat Globule-Epidermal Growth Factor-Factor 8 Reverses Lipopolysaccharide-Induced Microglial Oxidative Stress Oxid Med Cell Longev, 2019;2019(0):2601394. 2019 [PMID: 31001372] (In Vivo, Mouse) In Vivo Mouse
J Brelstaff, AM Tolkovsky, B Ghetti, M Goedert, MG Spillantin Living Neurons with Tau Filaments Aberrantly Expose Phosphatidylserine and Are Phagocytosed by Microglia Cell Rep, 2018;24(8):1939-1948.e4. 2018 [PMID: 30134156] (Western Blot, Mouse) Western Blot Mouse
AK Kumawat, C Yu, EA Mann, A Schridde, SC Finnemann, AM Mowat Expression and Characterisation of ?v?5 Integrin on Intestinal Macrophages Eur. J. Immunol., 2018;0(0):. 2018 [PMID: 29676784] (Bioassay, Mouse) Bioassay Mouse
MN Michalski, AL Seydel, EM Siismets, LE Zweifler, AJ Koh, BP Sinder, JI Aguirre, K Atabai, H Roca, LK McCauley Inflammatory bone loss associated with MFG-E8 deficiency is rescued by teriparatide FASEB J., 2018;0(0):fj201701238R. 2018 [PMID: 29475373] (Bioassay, Mouse) Bioassay Mouse
Y Wang, G Luo, J Chen, R Jiang, J Zhu, N Hu, W Huang, G Cheng, M Jia, B Su, N Zhang, T Cui Cigarette smoke attenuates phagocytic ability of macrophages through down-regulating Milk fat globule-EGF factor 8 (MFG-E8) expressions Sci Rep, 2017;7(0):42642. 2017 [PMID: 28195210] (Bioassay, Mouse) Bioassay Mouse
Mfge8 regulates enterocyte lipid storage by promoting enterocyte triglyceride hydrolase activity JCI Insight, 2016;1(18):e87418. 2016 [PMID: 27812539] (Bioassay, Mouse) Bioassay Mouse
A novel affinity-based method for the isolation of highly purified extracellular vesicles Sci Rep, 2016;6(0):33935. 2016 [PMID: 27659060] (Bioassay, Human) Bioassay Human
Marie-Joëlle Brissette Milk fat globule epidermal growth factor-8 limits tissue damage through inflammasome modulation during renal injury J Leukoc Biol, 2016;0(0):. 2016 [PMID: 27260955] (In Vivo, Mouse) In Vivo Mouse
MFG-E8 drives melanoma growth by stimulating mesenchymal stromal cell-induced angiogenesis and M2 polarization of tumor-associated macrophages Cancer Res, 2016;0(0):. 2016 [PMID: 27197197] (Bioassay, Mouse) Bioassay Mouse
Show All 25 Publications.

Reviews for MFG-E8 (2805-MF) (0)

There are no reviews for MFG-E8 (2805-MF). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for MFG-E8 (2805-MF) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Mouse MFG-E8 Protein and receive a gift card or discount.


Gene Symbol Mfge8