Recombinant Mouse MFG-E8 Protein

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Summary
Reactivity MuSpecies Glossary
Applications Binding Activity

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Recombinant Mouse MFG-E8 Protein Summary

Details of Functionality
Measured by the ability of the immobilized protein to support the adhesion of SVEC4‑10 mouse vascular endothelial cells. The ED50 for this effect is 10-50 ng/mL.
Source
Mouse myeloma cell line, NS0-derived mouse MFG-E8 protein
Ala23-Cys463, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ala23
Protein/Peptide Type
Recombinant Proteins
Gene
Mfge8
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
49.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
64-72 kDa, reducing conditions
Publications
Read Publications using
2805-MF in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Mouse MFG-E8 Protein

  • BA46
  • Breast epithelial antigen BA46
  • EDIL1
  • hP47
  • HsT19888
  • lactadherin
  • Lactahedrin
  • Medin
  • MFG1
  • MFGE8
  • MFG-E8
  • MFGM
  • milk fat globule-EGF factor 8 protein
  • Milk fat globule-EGF factor 8
  • O-acetyl disialoganglioside synthase
  • OAcGD3S
  • SED1
  • SPAG10
  • sperm associated antigen 10
  • sperm surface protein hP47

Background

Milk Fat Globulin Protein E8 (MFG-E8), also known as Lactadherin, MP47, breast epithelial antigen BA46, and SED1, is a 66‑75 kDa pleiotropic secreted glycoprotein that promotes mammary gland morphogenesis, angiogenesis, and tumor progression. MFG-E8 also plays an important role in tissue homeostasis and the prevention of inflammation (1). Mouse MGF-E8 contains two N-terminal EGF-like domains, a Pro/Thr-rich segment, and two C‑terminal F5/8-type discoidin-like domains (2). It MFG-E8 shares 63% and 94% aa sequence identity with comparable regions of human and rat MFG-E8, respectively. Alternative splicing of mouse MFG-E8 generates a short isoform lacking the Pro/Thr-rich region which contains sites for O-linked glycosylation and tyrosine sulfation (3). MFG-E8 is released into the milk in complex with lipid-containing milk fat globules. It is also found in multiple other cell types including endothelial cells and smooth muscle cells of the vasculature, immature dendritic cells, at the acrosomal cap of testicular and epididymal sperm, and in epithelial cells of the endometrium (1). MFG-E8 binds to the Integrins alpha V beta 3 and alpha V beta 5 and potentiates the angiogenic action of VEGF through VEGF R2 (4, 5). It reduces inflammation and tissue damage in a variety of settings. MFG-E8 functions as a bridge between phosphatidylserine on apoptotic cells and Integrin alpha V beta 3 on phagocytes, leading to the clearance of apoptotic debris (6). It mediates the engulfment of apoptotic bodies in atherosclerotic plaques and prion-infected brain (7, 8) and of apoptotic B cells during germinal center reactions (9, 10). MFG-E8 also promotes the removal of excess Collagen in fibrotic lungs and the regeneration of damaged intestinal epithelia (11, 12). Its tissue‑protective role impairs anti‑tumor immunity and chemotherapy‑induced apoptosis (13). MFG-E8 in the breastmilk blocks rotavirus infection in nursing babies (14).
  1. Raymond, A. et al. (2009) J. Cell. Biochem. 106:957.
  2. Stubbs, J.D. et al. (1990) Proc. Natl. Acad. Sci. USA 87:8417.
  3. Hoffhines, A.J. et al. (2008) J. Biol. Chem. 284:3096.
  4. Silvestre, J.-S. et al. (2005) Nat. Med. 11:499.
  5. Borges, E. et al. (2000) J. Biol. Chem. 275:39867.
  6. Hanayama, R. et al. (2002) Nature 417:182.
  7. Ait-Oufella, H. et al. (2007) Circulation 115:2168.
  8. Kranich, J. et al. (2010) J. Exp. Med. 207:2271.
  9. Hanayama, R. et al. (2004) Science 304:1147.
  10. Kranich, J. et al. (2010) J. Exp. Med. 205:1293.
  11. Atabai, K. et al. (2009) J. Clin. Invest. 119:3713.
  12. Bu, H.-F. et al. (2007) J. Clin. Invest. 117:3673.
  13. Jinushi, M. et al. (2009) J. Exp. Med. 206:1317.
  14. Kvistgaard, A.S. et al. (2004) J. Dairy Sci. 87:4088.

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Publications for MFG-E8 (2805-MF)(21)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Activity Assay, Bioassay, In Vivo.


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Activity Assay
(1)
Bioassay
(14)
In Vivo
(6)
All Applications
Filter By Species
Human
(1)
Mouse
(20)
All Species
Showing Publications 1 - 10 of 21. Show All 21 Publications.
Publications using 2805-MF Applications Species
AK Kumawat, C Yu, EA Mann, A Schridde, SC Finnemann, AM Mowat Expression and Characterisation of ?v?5 Integrin on Intestinal Macrophages Eur. J. Immunol., 2018;0(0):. 2018 [PMID: 29676784] (Bioassay, Mouse) Bioassay Mouse
Y Wang, G Luo, J Chen, R Jiang, J Zhu, N Hu, W Huang, G Cheng, M Jia, B Su, N Zhang, T Cui Cigarette smoke attenuates phagocytic ability of macrophages through down-regulating Milk fat globule-EGF factor 8 (MFG-E8) expressions Sci Rep, 2017;7(0):42642. 2017 [PMID: 28195210] (Bioassay, Mouse) Bioassay Mouse
Mfge8 regulates enterocyte lipid storage by promoting enterocyte triglyceride hydrolase activity JCI Insight, 2016;1(18):e87418. 2016 [PMID: 27812539] (Activity Assay, Mouse) Activity Assay Mouse
A novel affinity-based method for the isolation of highly purified extracellular vesicles Sci Rep, 2016;6(0):33935. 2016 [PMID: 27659060] (Bioassay, Human) Bioassay Human
Marie-Joëlle Brissette Milk fat globule epidermal growth factor-8 limits tissue damage through inflammasome modulation during renal injury J Leukoc Biol, 2016;0(0):. 2016 [PMID: 27260955] (In Vivo, Mouse) In Vivo Mouse
MFG-E8 drives melanoma growth by stimulating mesenchymal stromal cell-induced angiogenesis and M2 polarization of tumor-associated macrophages Cancer Res, 2016;0(0):. 2016 [PMID: 27197197] (Bioassay, Mouse) Bioassay Mouse
Correction of MFG-E8 Resolves Inflammation and Promotes Cutaneous Wound Healing in Diabetes J Immunol, 2016;0(0):. 2016 [PMID: 27194784] (Bioassay, Mouse) Bioassay Mouse
Cheyuo C, Aziz M, Yang W, Jacob A, Zhou M, Wang P Milk fat globule-EGF factor VIII attenuates CNS injury by promoting neural stem cell proliferation and migration after cerebral ischemia. PLoS ONE, 2015;10(4):e0122833. 2015 [PMID: 25867181] (Bioassay, Mouse) Bioassay Mouse
Uchiyama A, Yamada K, Perera B, Ogino S, Yokoyama Y, Takeuchi Y, Ishikawa O, Motegi S Protective effect of MFG-E8 after cutaneous ischemia-reperfusion injury. J Invest Dermatol, 2015;135(4):1157-65. 2015 [PMID: 25493650] (Bioassay, Mouse) Bioassay Mouse
Soki F, Koh A, Jones J, Kim Y, Dai J, Keller E, Pienta K, Atabai K, Roca H, McCauley L Polarization of prostate cancer-associated macrophages is induced by milk fat globule-EGF factor 8 (MFG-E8)-mediated efferocytosis. J Biol Chem, 2014;289(35):24560-72. 2014 [PMID: 25006249] (Bioassay, Mouse) Bioassay Mouse
Show All 21 Publications.

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Bioinformatics

Gene Symbol Mfge8
Uniprot