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Dihydrolipoamide Dehydrogenase/DLD Antibody

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Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Wild-type (WT) and DLD knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:500. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Wild-type (WT) and DLD knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody ...read more
Immunocytochemistry/ Immunofluorescence: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: DLD protein stained by DLD antibody diluted at 1:500. Blue: ...read more
Immunohistochemistry-Paraffin: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Human gastric cancer, using DLD antibody at 1:100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Sample (50 ug of whole cell lysate) A: Mouse brain 10% SDS PAGE diluted at 1:1000.
Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Sample(30 ug of whole cell lysate) A:293T 10% SDS PAGE, antibody diluted at 1:1000.
Western Blot: Dihydrolipoamide Dehydrogenase/DLD Antibody [NBP1-31302] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with DLD antibody diluted at 1:1000.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, Bv, Ca, Ch, XpSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

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Dihydrolipoamide Dehydrogenase/DLD Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the center region of human DLD. The exact sequence is proprietary.
Localization
Mitochondrion matrix
Predicted Species
Porcine (99%), Bovine (98%), Chicken (93%), Canine (98%), Xenopus (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DLD
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 4
using
NBP1-31302 in the following applications:

Reactivity Notes

Xenopus laevis (94%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7), 20% Glycerol
Preservative
0.01% Thimerosal
Purity
Protein A purified

Alternate Names for Dihydrolipoamide Dehydrogenase/DLD Antibody

  • 2-oxo-glutarate complex, branched chain keto acid dehydrogenase complex)
  • branched chain keto acid dehydrogenase complex
  • Diaphorase
  • dihydrolipoamide dehydrogenase (E3 component of pyruvate dehydrogenase complex
  • Dihydrolipoamide Dehydrogenase
  • dihydrolipoamide dehydrogenasemitochondrial
  • DLD
  • DLDD
  • DLDH
  • E3 component of pyruvate dehydrogenase complex, 2-oxo-glutarate complex
  • E3
  • EC 1.8.1
  • EC 1.8.1.4
  • GCSL
  • Glycine cleavage system L protein
  • glycine cleavage system protein L
  • LAD
  • Lipoamide Dehydrogenase
  • lipoamide reductase
  • lipoyl dehydrogenase
  • PHE3

Background

This gene encodes the L protein of the mitochondrial glycine cleavage system. The L protein, also named dihydrolipoamide dehydrogenase, is also a component of the pyruvate dehydrogenase complex, the alpha-ketoglutarate dehydrogenase complex, and the branched-chain alpha-keto acide dehydrogenase complex. Mutations in this gene have been identified in patients with E3-deficient maple syrup urine disease and lipoamide dehydrogenase deficiency.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: UBE4B Antibody [NBP1-84943] - Lane 1: NIH-3T3 cell lysate (Mouse embryonic fibroblast cells) Lane 2: NBT-II cell lysate (Rat Wistar bladder tumour cells)
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Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line and Raji human Burkitt's lymphoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Catalase at approximately 62 kDa (as indicated) using 5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Catalase knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human/Mouse/Rat Catalase Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3398) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Catalase at approximately 64 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Western Blot: ME1 Antibody [NBP1-32398] - Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ME1 antibody diluted at 1:1000. HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
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Western Blot: ME1 Antibody [NBP1-32398] - Sample (30 ug of whole cell lysate) A: NIH-3T3 B: JC C: BCL-1 7. 5% SDS PAGE; antibody diluted at 1:1000.
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Dihydrolipoamide
Dehydrogenase/DLD Ant ...
NBP1-31302
Dihydrolipoamide Dehydrogenase/DLD Antibody
Species: Hu, Mu, Rt, Po, Bv, Ca, Ch, Xp
Applications: WB, ICC/IF, IHC, IHC-P
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Review for Dihydrolipoamide Dehydrogenase/DLD Antibody (NBP1-31302) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP1-31302:
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Western Blot Dihydrolipoamide Dehydrogenase/DLD NBP1-31302
Enlarge 		4
reviewed by:
Hongxia Hu
 WB Human 01/09/2011
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Summary

ApplicationWestern Blot
Sample TestedHela whole cell lysate, Sample Amount: 10ug
SpeciesHuman
CommentsExpected specific band but there is black dots on the blot. Looks dirty.

Blocking

Blocking DetailsBlocking Buffer: 5% Milk in TBST, Blocking Time: 1 hour, Blocking Temp: Room temperature

Primary Anitbody

Dilution RatioPrimary Ab Dilution Ratio: 1:1000, Incubation Dilution Buffer: 5% Milk in TBST, Incubation Time: overnight, Incubation Temp: 4C

Secondary Antibody

Secondary DescriptionSecondary Ab: anti-rabbit HRP, Secondary Ab Dilution Ratio: 1:5000

Details

Detection NotesDetection Method: ECL Plus, Exposure Time: 5 minutes, Specific Bands: ~58 kDa

Comments

CommentsExpected specific band but there is black dots on the blot. Looks dirty.

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Western Blot Dihydrolipoamide Dehydrogenase/DLD NBP1-31302
Enlarge
 Hongxia Hu
01/09/2011
Application: WB
Species: Human
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Bioinformatics

Gene Symbol DLD