E-Cadherin Antibody (CL1180) - Azide and BSA Free

Analysis in human duodenum and cerebral cortex tissues using NBP3-43889 antibody. Corresponding E-Cadherin RNA-seq data are presented for the same tissues.

Staining of BEWO cells using the Anti-E-Cadherin monoclonal antibody) .

Staining of human cerebral cortex shows no positivity in neuronal cells as expected.

Staining of human colorectal cancer shows strong membranous positivity in tumor cells.

Staining of human duodenum shows moderate membranous positivity in glandular cells.

Staining of human prostate shows moderate membranous positivity in glandular cells.

Analysis in MCF-7 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-E-Cadherin antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: CL1180
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

E-Cadherin Antibody (CL1180) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse E-Cadherin Antibody (CL1180) - Azide and BSA Free (NBP2-34477) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of P12830, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: CDH1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 2-10 ug/ml
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for E-Cadherin Antibody (CL1180) - Azide and BSA Free

• Arc-1
• CAD1
• cadherin 1, E-cadherin (epithelial)
• cadherin 1, type 1, E-cadherin (epithelial)
• Cadherin-1
• calcium-dependent adhesion protein, epithelial
• CAM 120/80
• CD324 antigen
• CD324
• CDH1
• CDHE
• cell-CAM 120/80
• Cell-CAM120/80
• ECAD
• ECadherin
• E-Cadherin
• Epithelial cadherin
• LCAM
• L-CAM
• UVOE-Cadherin
• Uvomorulin

Background

E-cadherin, or epithelial cadherin, is an cell-cell adhesion glycoprotein important to cellular processes such as morphology, polarity, development, tissue integrity and migration. E cadherin's extracellular domain interacts with other E-cadherin protein on adjacent epithelial cells to establish adhesion between them. E cadherin has also been identified as a tumor and metastasis suppressor, and its mutations have been linked to various forms of cancer.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CDH1