Dtk/TYRO3 Antibody

Images

 
Western Blot: Dtk/TYRO3 Antibody [NBP1-28635] - Lane 1: Western blot on normal human brain, Lane 2: Western blot on normal mouse brain.
Immunocytochemistry/ Immunofluorescence: Dtk/TYRO3 Antibody [NBP1-28635] - Staining of HELA cells using NBP128635 (Green) at a 1:200 dilution detected using Dylight-488 conjugated goat anti-rabbit secondary antibody. ...read more
Flow Cytometry: Dtk/TYRO3 Antibody [NBP1-28635] - An intracellular stain was performed on HeLa cells with Dtk/TYRO3 Antibody NBP1-28635AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA ...read more
Flow (Intracellular): Dtk/TYRO3 Antibody [NBP1-28635] - An intracellular stain was performed on U-937 cells with Dtk/TYRO3 Antibody NBP1-28635AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% ...read more

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Flow, Flow-IC, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.19 mg/ml

Order Details

Dtk/TYRO3 Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of the human TYRO3 protein (within residues 100-250). [Swiss-Prot# Q06418]
Localization
Cell membrane; Single-pass type I membrane protein.
Predicted Species
Rat (94%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TYRO3
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence 1:200
  • Flow (Intracellular)
Application Notes
This TYRO3 antibody is useful for Immunocytochemistry/Immunofluorescence and Western blot, where a band can be seen at ~96 kDa. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. Use in FLOW cytometry reported in scientific literature ( PMID 27034374).
Theoretical MW
96 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
Mouse Brain Whole Tissue Lysate (Adult Whole Normal)
Human Brain Whole Tissue Lysate (Adult Whole Normal)
Dtk/TYRO3 Knockout HeLa Cell Lysate
Control Peptide
Dtk/TYRO3 Peptide (NBP1-28635PEP)
Publications
Read Publications using
NBP1-28635 in the following applications:

Reactivity Notes

Human and mouse. Immunogen sequence has 94% identity to rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.19 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Dtk/TYRO3 Antibody

  • Brt
  • BYK
  • Dtk
  • EC 2.7.10
  • EC 2.7.10.1
  • FLJ16467
  • Rse
  • Sky
  • Tif
  • TYRO3 protein tyrosine kinase
  • Tyro3
  • Tyrosine-protein kinase byk
  • Tyrosine-protein kinase DTK
  • tyrosine-protein kinase receptor TYRO3
  • Tyrosine-protein kinase RSE
  • Tyrosine-protein kinase SKY

Background

TYRO3 (tyrosine-protein kinase receptor TYRO3) is a RTK which facilitate transduction of signals from extracellular matrix (ECM) into cytoplasm via its direct interaction with multiple ligands such as TUB, TULP1 or GAS6 for the regulation of multiple biological processes such as cell survival, migration and differentiation. TYRO3-ligand binding stimulates its dimerization as well as autophosphorylation on its intracellular domain that provides docking sites for the molecules of downstream signaling. Once stimulated, TYRO3 interacts with PIK3R1 leading to increased PI3K activity followed by AKT signaling activation including NFkB nuclear translocation mediated up-regulation of transcription of NFkB's target genes. TYRO3 signaling events are critical to neuron protection from excitotoxic injury, platelet aggregation, cytoskeletal reorganization, inhibition of TLRs-mediated innate immune response via STAT1 activation followed by production of cytokine signaling suppressors (SOCS1 and SOCS3). TYRO3 is highly expressed during CNS neurogenesis with distinct patterns in adult brain and has been closely related to CNS immunodysfunction.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Dtk/TYRO3 Antibody (NBP1-28635)(3)

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Product General Protocols

View specific protocols for Dtk/TYRO3 Antibody (NBP1-28635): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Dtk/TYRO3 Antibody (NBP1-28635) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-28635AF350
Alexa Fluor 405 NBP1-28635AF405
Alexa Fluor 488 NBP1-28635AF488
Alexa Fluor 532 NBP1-28635AF532
Alexa Fluor 594 NBP1-28635AF594
Alexa Fluor 647 NBP1-28635AF647
Alexa Fluor 700 NBP1-28635AF700
Alexa Fluor 750 NBP1-28635AF750
Allophycocyanin/Cy7 NBP1-28635APCCY7
Biotin NBP1-28635B
DyLight 350 NBP1-28635UV
DyLight 405 NBP1-28635V
DyLight 488 NBP1-28635G
DyLight 550 NBP1-28635R
DyLight 594 NBP1-28635DL594
DyLight 650 NBP1-28635C
DyLight 680 NBP1-28635FR
DyLight 755 NBP1-28635IR
FITC NBP1-28635F
HRP NBP1-28635H
Janelia Fluor 549 NBP1-28635JF549
Janelia Fluor 646 NBP1-28635JF646
PE/Atto594 NBP1-28635PEATT594
PE/Cy5.5 NBP1-28635PECY55
PE/Cy7 NBP1-28635PECY7

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Bioinformatics

Gene Symbol TYRO3
Entrez
Uniprot