Recombinant Human Gas6 Protein, CF

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Recombinant Human Gas6 885-GSB down regulates the expression of Axl in DU145 human prostate carcinoma cells. The ED50 for this effect is 25-150 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity
Format
Carrier-Free

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Recombinant Human Gas6 Protein, CF Summary

Details of Functionality
Measured by its ability to down regulate the expression of Axl in DU145 human prostate carcinoma cells. Mishra, A. et al. (2012) Mol. Cancer Res. 10:703. The ED50 for this effect is 25-150 ng/mL
Source
Mouse myeloma cell line, NS0-derived human Gas6 protein
Ala49-Ala678, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Ala49
Protein/Peptide Type
Recombinant Proteins
Gene
GAS6
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Endotoxin Note
<1.0 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
70.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
70-85 kDa, reducing conditions
Reviewed Applications
Read 1 Review rated 4
using
885-GSB in the following application:

Publications
Read Publications using
885-GSB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and Citrate.
Purity
>90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Reconstitution Instructions
Reconstitute at 500 μg/mL in sterile water.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human Gas6 Protein, CF

  • AXLLG
  • AXLLGAXL stimulatory factor
  • AXSFAXL receptor tyrosine kinase ligand
  • DKFZp666G247
  • FLJ34709
  • Gas6
  • GAS-6
  • growth arrest-specific 6
  • growth arrest-specific protein 6

Background

Gas6 (Growth Arrest Specific 6) is a multimodular protein that is up-regulated by a wide variety of cell types in response to growth arrest (1). Gas6 and the structurally related Protein S are vitamin K-dependent and have an extensively gamma -carboxylated N-terminal Gla domain, four EGF-like repeats, and a C-terminal region with homology to steroid hormone binding globulin (SHBG) (2). Human Gas6 is a 75 kDa protein that shares 77%-79% aa sequence identity with mouse and rat Gas6, and 43% aa identity with human protein S (over the region expressed). Alternate splicing generates isoforms that lack the Gla domain and/or the spacer between the
EGF‑like and SHBG regions. Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer (3-5). Human Gas6 interacts with both mouse and rat orthologs of these receptors (1). The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl (6). Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors (7, 8). Gas6 induces a variety of responses, including prevention of apoptosis (9), cell proliferation (10), platelet-mediated thrombosis (11), retinal epithelial cell phagocytosis of outer rod segments (12), inhibition of VEGF-induced endothelial cell chemotaxis (13), and the differentiation and expansion of NK cell precursors (14). The affinity of Gas6 for phosphatidylserine likely contributes to its role in promoting the phagocytosis of apoptotic cells (15). Several of these effects have been shown to require gamma -carboxylation of the Gla domain (12, 16).
  1. Hafizi, S. and B. Dahlback (2006) FEBS J. 273:5231.
  2. Manfioletti, G. et al. (1993) Mol. Cell Biol. 13:4976.
  3. Stitt, T.N. et al. (1995) Cell 80:661.
  4. Ohashi, K. et al. (1995) J. Biol. Chem. 270:22681.
  5. Nagata, K. et al. (1996) J. Biol. Chem. 271:30022.
  6. Goruppi, S. et al. (1997) FEBS Lett. 415:59.
  7. Sather, S. et al. (2007) Blood 109:1026.
  8. Budagian, V. et al. (2005) Mol. Cell. Biol. 25:9324.
  9. Shankar, S.L. et al. (2006) J. Neurosci. 26:5638.
  10. Yanagita, M. et al. (2002) J. Clin. Invest. 110:239.
  11. Gould, W.R. et al. (2005) J. Thromb. Haemost. 3:733.
  12. Hall, M.O. et al. (2002) Exp. Eye Res. 75:391.
  13. Gallicchio, M. et al. (2005) Blood 105:1970.
  14. Caraux, A. et al. (2006) Nat. Immunol. 7:747.
  15. Wu, Y. et al. (2005) J. Cell Sci. 118:539.
  16. Hasanbasic, I. et al. (2005) J. Thromb. Haemost. 3:2790.

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Publications for Gas6 (885-GSB)(30)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 7 applications: Bioassay, Cell Culture, ELISA (Capture), ELISA (Standard), ELISA Standard, Flow Cytometry, Western Blot.


Filter By Application
Bioassay
(20)
Cell Culture
(4)
ELISA (Capture)
(1)
ELISA (Standard)
(1)
ELISA Standard
(1)
Flow Cytometry
(1)
Western Blot
(1)
All Applications
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Human
(25)
Mouse
(1)
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Showing Publications 1 - 10 of 30. Show All 30 Publications.
Publications using 885-GSB Applications Species
M Iida, NK McDaniel, KL Kostecki, NB Welke, CA Kranjac, P Liu, C Longhurst, JY Bruce, S Hong, R Salgia, DL Wheeler AXL regulates neuregulin1 expression leading to cetuximab resistance in head and neck cancer BMC Cancer, 2022;22(1):447. 2022 [PMID: 35461210] (Bioassay, Human) Bioassay Human
A Puddu, S Ravera, I Panfoli, N Bertola, D Maggi High Glucose Impairs Expression and Activation of MerTK in ARPE-19 Cells International Journal of Molecular Sciences, 2022;23(3):. 2022 [PMID: 35163068] (Bioassay, Human) Bioassay Human
LW Sun, SH Kao, SF Yang, SW Jhang, YC Lin, CM Chen, YH Hsieh Corosolic Acid Attenuates the Invasiveness of Glioblastoma Cells by Promoting CHIP-Mediated AXL Degradation and Inhibiting GAS6/AXL/JAK Axis Cells, 2021;10(11):. 2021 [PMID: 34831142] (Bioassay, Human) Bioassay Human
H Chang, R An, X Li, X Lang, J Feng, M Lv Anti-Axl monoclonal antibodies attenuate the migration of MDA-MB-231 breast cancer cells Oncology Letters, 2021;22(5):749. 2021 [PMID: 34539853] (ELISA (Capture), Human) ELISA (Capture) Human
X Wu, L Wang, NA Pearson, S Renuse, R Cheng, Y Liang, DG Mun, AK Madugundu, Y Xu, PS Gill, A Pandey Quantitative Tyrosine Phosphoproteome Profiling of AXL Receptor Tyrosine Kinase Signaling Network Cancers, 2021;13(16):. 2021 [PMID: 34439388] (Bioassay, Human) Bioassay Human
S Pietrobono, G Anichini, C Sala, F Manetti, LL Almada, S Pepe, RM Carr, BD Paradise, JN Sarkaria, JI Davila, L Tofani, I Battisti, G Arrigoni, L Ying, C Zhang, H Li, A Meves, ME Fernandez-, B Stecca ST3GAL1 is a target of the SOX2-GLI1 transcriptional complex and promotes melanoma metastasis through AXL Nat Commun, 2020;11(1):5865. 2020 [PMID: 33203881] (Bioassay, Human) Bioassay Human
W Du, J Zhu, Y Zeng, T Liu, Y Zhang, T Cai, Y Fu, W Zhang, R Zhang, Z Liu, JA Huang KPNB1-mediated nuclear translocation of PD-L1 promotes non-small cell lung cancer cell proliferation via the Gas6/MerTK signaling pathway Cell Death Differ, 2020;0(0):. 2020 [PMID: 33139930] (Bioassay, Human) Bioassay Human
J Xue, L Xu, H Zhu, M Bai, X Li, Z Zhao, H Zhong, G Cheng, X Li, F Hu, Y Su CD14+CD16- monocytes are the main precursors of osteoclasts in rheumatoid arthritis via expressing Tyro3TK Arthritis Research & Therapy, 2020;22(1):221. 2020 [PMID: 32958023] (Bioassay, Human) Bioassay Human
A Abu-Thurai, MA Goyette, J Boulais, C Delliaux, C Apcher, C Schott, R Chidiac, H Bagci, MP Thibault, D Davidson, M Ferron, A Veillette, RJ Daly, AC Gingras, JP Gratton, JF Côté AXL confers cell migration and invasion by hijacking a PEAK1-regulated focal adhesion protein network Nat Commun, 2020;11(1):3586. 2020 [PMID: 32681075] (ELISA Standard, Human) ELISA Standard Human
S Alivernini, L MacDonald, A Elmesmari, S Finlay, B Tolusso, MR Gigante, L Petricca, C Di Mario, L Bui, S Perniola, M Attar, M Gessi, AL Fedele, S Chilaka, D Somma, SN Sansom, A Filer, C McSharry, NL Millar, K Kirschner, A Nerviani, MJ Lewis, C Pitzalis, AR Clark, G Ferracciol, I Udalova, CD Buckley, E Gremese, IB McInnes, TD Otto, M Kurowska-S Distinct synovial tissue macrophage subsets regulate inflammation and remission in rheumatoid arthritis Nat. Med., 2020;0(0):. 2020 [PMID: 32601335] (Flow Cytometry, Human) Flow Cytometry Human
Show All 30 Publications.

Review for Gas6 (885-GSB) (1) 41

Average Rating: 4
(Based on 1 review)

Reviews using 885-GSB:
Filter by Applications
Binding assay/Protein-protein interaction
(1)
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  4
reviewed by:
Serena Adam
Binding assay/Protein-protein interaction 03/21/2018
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Summary

ApplicationBinding assay/Protein-protein interaction
LotDFGX0416121

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Serena Adam
03/21/2018
Application: Binding assay/Protein-protein interaction
Species:

Bioinformatics

Gene Symbol GAS6
Uniprot