Axl Antibody [Unconjugated]

Axl was detected in immersion fixed paraffin-embedded sections of mouse kidney using Goat Anti-Mouse Axl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF854) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to cell membranes in convoluted tubules. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

• Reactivity: Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

Axl Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Axl
  His20-Pro443
  Accession # Q80YQ3
• Specificity: Detects mouse Axl in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 10% cross‑reactivity with recombinant human Axl is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: AXL
• Purity Statement: Antigen Affinity-purified
• Endotoxin Note: <0.10 EU per 1 μg of the antibody by the LAL method.

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3-15 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Axl Antibody [Unconjugated]

• AI323647
• ARK
• AXL oncogene
• AXL receptor tyrosine kinase
• AXL transforming sequence/gene
• Axl
• EC 2.7.10
• EC 2.7.10.1
• JTK11
• Tyro7
• tyrosine-protein kinase receptor UFO
• UFO
• UFOoncogene AXL

Background

Axl (Ufo, Ark), Dtk (Sky, Tyro3, Rse, Brt), and Mer (human and mouse homologues of chicken c-Eyk) constitute a subfamily of the receptor tyrosine kinases (1, 2). The extracellular domains of these proteins contain two Ig-like motifs and two fibronectin type III motifs. This characteristic topology is also found in neural cell adhesion molecules and in receptor tyrosine phosphatases. The mouse Axl cDNA encodes an 888 amino acid (aa) precursor that includes an 18 aa signal sequence, a 427 aa extracellular domain, a 21 aa transmembrane segment, and a 422 aa cytoplasmic domain. The extracellular domains of mouse and human Axl share 81% aa sequence identity. These receptors bind the vitamin K-dependent protein growth arrest specific gene 6 (Gas6) which is structurally related to the anticoagulation factor protein S. Binding of Gas6 induces receptor autophosphorylation and downstream signaling pathways that can lead to cell proliferation, migration, or the prevention of apoptosis (3). This family of tyrosine kinase receptors is involved in hematopoiesis, embryonic development, tumorigenesis, and regulation of testicular functions.

1. Yanagita, M. (2004) Curr. Opin. Nephrol. Hypertens. 13:465.
2. Nagata, K. et al. (1996) J. Biol. Chem. 22:30022.
3. Holland, S. et al. (2005) Canc. Res. 65:9294.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Axl Antibody (AF854)(27)

Publications using AF854

• B van Lenger, L Zhan, D Xia, D Chan, D Joy, JI Park, D Tatarakis, M Calvert, S Hummel, S Lianoglou, ME Pizzo, R Prorok, E Thomsen, LM Bartos, P Beumers, A Capell, SS Davis, L de Weerd, JC Dugas, J Duque, T Earr, K Gadkar, T Giese, A Gill, J Gnörich, C Ha, M Kannuswamy, DJ Kim, ST Kunte, LH Kunze, D Lac, K Lechtenber, AW Leung, CC Liang, I Lopez, P McQuade, A Modi, VO Torres, HN Nguyen, I Pesämaa, N Propson, M Reich, Y Robles-Col, K Schlepckow, L Slemann, H Solanoy, JH Suh, RG Thorne, C Vieira, K Wind-Mark, K Xiong, YJY Zuchero, D Diaz, MS Dennis, F Huang, K Scearce-Le, RJ Watts, C Haass, JW Lewcock, G Di Paolo, M Brendel, PE Sanchez, KM Monroe A TREM2-activating antibody with a blood-brain barrier transport vehicle enhances microglial metabolism in Alzheimer&#039;s disease models Nature Neuroscience, 2023-01-12;0(0):. 2023-01-12 [PMID: 36635496] (IHC, Mouse)
• A Ko, M Hasanain, YT Oh, F D'Angelo, D Sommer, B Frangaj, S Tran, F Bielle, B Pollo, R Paterra, K Mokhtari, RK Soni, M Peyre, M Eoli, L Papi, M Kalamaride, M Sanson, A Iavarone, A Lasorella LZTR1 mutation mediates oncogenesis through stabilization of EGFR and AXL Cancer Discovery, 2023-03-01;0(0):. 2023-03-01 [PMID: 36445254] (IHC, Western Blot, Transgenic Mouse)
• Y Shen, Q Zhu, M Xiao, L Yin, W Feng, J Feng, J He, P Li, X Chen, W Ding, J Zhong, Z Zeng, Z Xie, J Liu, X Zu Inhibitory effect of the novel tyrosine kinase inhibitor DCC-2036 on triple-negative breast cancer stem cells through AXL-KLF5 positive feedback loop Cell Death & Disease, 2022-08-30;13(8):749. 2022-08-30 [PMID: 36042208] (ICC, Mouse)
• H Jung, SY Lee, S Lim, HR Choi, Y Choi, M Kim, S Kim, Y Lee, KH Han, WS Chung, CH Kim Anti-inflammatory clearance of amyloid-beta by a chimeric Gas6 fusion protein Nature Medicine, 2022-08-04;0(0):. 2022-08-04 [PMID: 35927581] (Western Blot, Mouse)
• ME Fane, Y Chhabra, GM Alicea, DA Maranto, SM Douglass, MR Webster, VW Rebecca, GE Marino, F Almeida, BL Ecker, DJ Zabransky, L Hüser, T Beer, HY Tang, A Kossenkov, M Herlyn, DW Speicher, W Xu, X Xu, EM Jaffee, JA Aguirre-Gh, AT Weeraratna Stromal changes in the aged lung induce an emergence from melanoma dormancy Nature, 2022-06-01;606(7913):396-405. 2022-06-01 [PMID: 35650435] (Western Blot, Mouse)
• F Wanke, S Gutbier, A Rümmelin, M Steinberg, LD Hughes, M Koenen, J Komuczki, D Regan-Komi, S Wagage, J Hesselmann, R Thoma, D Brugger, T Christopei, H Wang, F Point, R Hallet, S Ghosh, CV Rothlin, C Patsch, B Geering Ligand-dependent kinase activity of MERTK drives efferocytosis in human iPSC-derived macrophages Cell Death & Disease, 2021-05-25;12(6):538. 2021-05-25 [PMID: 34035216] (Flow Cytometry, Human, Mouse)
• Y Huang, KE Happonen, PG Burrola, C O'Connor, N Hah, L Huang, A Nimmerjahn, G Lemke Microglia use TAM receptors to detect and engulf amyloid &amp;beta plaques Nature Immunology, 2021-04-15;0(0):. 2021-04-15 [PMID: 33859405] (IHC, Mouse)
• JM Rosin, CM Marsters, F Malik, R Far, L Adnani, C Schuurmans, QJ Pittman, DM Kurrasch Embryonic Microglia Interact with Hypothalamic Radial Glia during Development and Upregulate the TAM Receptors MERTK and AXL following an Insult Cell Reports, 2021-01-05;34(1):108587. 2021-01-05 [PMID: 33406432] (IHC, Mouse)
• C Madore, Q Leyrolle, L Morel, M Rossitto, AD Greenhalgh, JC Delpech, M Martinat, C Bosch-Bouj, J Bourel, B Rani, C Lacabanne, A Thomazeau, KE Hopperton, S Beccari, A Sere, A Aubert, V De Smedt-P, C Lecours, K Bisht, L Fourgeaud, S Gregoire, L Bretillon, N Acar, NJ Grant, J Badaut, P Gressens, A Sierra, O Butovsky, ME Tremblay, RP Bazinet, C Joffre, A Nadjar, S Layé Essential omega-3 fatty acids tune microglial phagocytosis of synaptic elements in the mouse developing brain Nature Communications, 2020-11-30;11(1):6133. 2020-11-30 [PMID: 33257673] (Western Blot, Mouse)
• JC DuBois, AK Ray, P Davies, B Shafit-Zag Anti-Axl antibody treatment reduces the severity of experimental autoimmune encephalomyelitis J Neuroinflammation, 2020-10-29;17(1):324. 2020-10-29 [PMID: 33121494] (Functional Assay, Mouse)
• LA Hohsfield, AR Najafi, Y Ghorbanian, N Soni, EE Hingco, SJ Kim, AD Jue, V Swarup, MA Inlay, KN Green Effects of long-term and brain-wide colonization of peripheral bone marrow-derived myeloid cells in the CNS Journal of Neuroinflammation, 2020-09-20;17(1):279. 2020-09-20 [PMID: 32951604] (IHC, Mouse)
• N Eder, F Roncaroli, MC Dolmart, S Horswell, F Andreiuolo, HR Flynn, AT Lopes, S Claxton, JP Kilday, L Collinson, JH Mao, T Pietsch, B Thompson, AP Snijders, SK Ultanir YAP1/TAZ drives ependymoma-like tumour formation in mice Nat Commun, 2020-05-13;11(1):2380. 2020-05-13 [PMID: 32404936] (IHC, Mouse)
• A Tutusaus, E de Gregori, B Cucarull, H Cristóbal, C Aresté, I Graupera, M Coll, A Colell, G Gausdal, JB Lorens, P García de, A Morales, M Marí A FUNCTIONAL ROLE OF GAS6/TAM IN NON-ALCOHOLIC STEATOHEPATITIS PROGRESSION IMPLICATES AXL AS THERAPEUTIC TARGET Cell Mol Gastroenterol Hepatol, 2019-11-02;0(0):. 2019-11-02 [PMID: 31689560] (Cell Culture, Mouse)
• L Landolt, J Furriol, J Babickova, L Ahmed, Ø Eikrem, T Skogstrand, A Scherer, S Suliman, S Leh, JB Lorens, G Gausdal, HP Marti, T Osman AXL targeting reduces fibrosis development in experimental unilateral ureteral obstruction Physiol Rep, 2019-05-01;7(10):e14091. 2019-05-01 [PMID: 31134766] (IHC, Western Blot, Mouse)
• P Ayata, A Badimon, HJ Strasburge, MK Duff, SE Montgomery, YE Loh, A Ebert, AA Pimenova, BR Ramirez, AT Chan, JM Sullivan, I Purushotha, JR Scarpa, AM Goate, M Busslinger, L Shen, B Losic, A Schaefer Epigenetic regulation of brain region-specific microglia clearance activity Nat. Neurosci., 2018-07-23;21(8):1049-1060. 2018-07-23 [PMID: 30038282] (Western Blot, Mouse)
• A Brosius Lu, WS Chung, SA Sloan, GA Carson, L Zhou, E Lovelett, S Posada, JB Zuchero, BA Barres Schwann cells use TAM receptor-mediated phagocytosis in addition to autophagy to clear myelin in a mouse model of nerve injury Proc. Natl. Acad. Sci. U.S.A., 2017-09-05;0(0):. 2017-09-05 [PMID: 28874532] (IHC, Western Blot, Mouse)
• Y Yanagihash, K Segawa, R Maeda, YI Nabeshima, S Nagata Mouse macrophages show different requirements for phosphatidylserine receptor Tim4 in efferocytosis Proc. Natl. Acad. Sci. U.S.A., 2017-08-02;0(0):. 2017-08-02 [PMID: 28768810] (Western Blot, Mouse)
• M Kurowska-S, S Alivernini, EG Melchor, A Elmesmari, B Tolusso, C Tange, L Petricca, DS Gilchrist, G Di Sante, C Keijzer, L Stewart, C Di Mario, V Morrison, JM Brewer, D Porter, S Milling, RD Baxter, D McCarey, E Gremese, G Lemke, G Ferracciol, C McSharry, IB McInnes MicroRNA-34a dependent regulation of AXL controls the activation of dendritic cells in inflammatory arthritis Nat Commun, 2017-06-22;8(0):15877. 2017-06-22 [PMID: 28639625] (Western Blot, Mouse)
• A Voronova, SA Yuzwa, BS Wang, S Zahr, C Syal, J Wang, DR Kaplan, FD Miller Migrating Interneurons Secrete Fractalkine to Promote Oligodendrocyte Formation in the Developing Mammalian Brain Neuron, 2017-05-03;94(3):500-516.e9. 2017-05-03 [PMID: 28472653] (Neutralization, Mouse)
• Opposing Roles of Tyrosine Kinase Receptors Mer and Axl Determine Clinical Outcomes in Experimental Immune-Mediated Nephritis J Immunol, 2016-08-15;0(0):. 2016-08-15 [PMID: 27527599] (Western Blot, Mouse)
• L Fourgeaud, PG Través, Y Tufail, H Leal-Baile, ED Lew, PG Burrola, P Callaway, A Zagórska, CV Rothlin, A Nimmerjahn, G Lemke TAM receptors regulate multiple features of microglial physiology Nature, 2016-04-06;532(7598):240-4. 2016-04-06 [PMID: 27049947] (Western Blot, Mouse)
• Savage J, Jay T, Goduni E, Quigley C, Mariani M, Malm T, Ransohoff R, Lamb B, Landreth G Nuclear receptors license phagocytosis by trem2+ myeloid cells in mouse models of Alzheimer&#039;s disease. J Neurosci, 2015-04-22;35(16):6532-43. 2015-04-22 [PMID: 25904803] (IHC, Mouse)
• Zagorska A, Traves P, Lew E, Dransfield I, Lemke G Diversification of TAM receptor tyrosine kinase function. Nat Immunol, 2014-09-07;15(10):920-8. 2014-09-07 [PMID: 25194421] (Immunoprecipitation, Neutralization, Mouse)
• Identification of Axl as a downstream effector of TGF-beta1 during Langerhans cell differentiation and epidermal homeostasis. J. Exp. Med., 2012-10-15;209(11):2033-47. 2012-10-15 [PMID: 23071254] (IHC-Fr, Mouse)
• Zhong Z, Wang Y, Guo H, Sagare A, Fernandez JA, Bell RD, Barrett TM, Griffin JH, Freeman RS, Zlokovic BV Protein S protects neurons from excitotoxic injury by activating the TAM receptor Tyro3-phosphatidylinositol 3-kinase-Akt pathway through its sex hormone-binding globulin-like region. J. Neurosci., 2010-11-17;30(46):15521-34. 2010-11-17 [PMID: 21084607] (ICC, Western Blot, Mouse)
• Zhu D, Wang Y, Singh I Protein S controls hypoxic/ischemic blood-brain barrier disruption through the TAM receptor Tyro3 and sphingosine 1-phosphate receptor. Blood, 2010-03-26;115(23):4963-72. 2010-03-26 [PMID: 20348395] (Western Blot, Mouse)
• Gould WR, Baxi SM, Schroeder R, Peng YW, Leadley RJ, Peterson JT, Perrin LA Gas6 receptors Axl, Sky and Mer enhance platelet activation and regulate thrombotic responses. J. Thromb. Haemost., 2005-02-23;3(4):733-41. 2005-02-23 [PMID: 15733062] (Flow Cytometry, Mouse)

Reviews for Axl Antibody (AF854) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Mouse.

reviewed by: Christian Schuerch IHC-Fr Mouse 04/12/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Frozen mouse spleen
• Species: Mouse
• Lot: I don't have it any more

reviewed by: Juwen DuBois in vivo Mouse 04/03/2017

Summary

• Application: Other
• Sample Tested: brain and spinal cord
• Species: Mouse
• Lot: ctc0216081

Comments

• Comments: Used in vivo to activate Axl in the CNS.

Bioinformatics

• Gene Symbol: AXL
• Uniprot:
  • Q80YQ3()