S100A10 Antibody [Unconjugated]

Western blot shows lysates of mouse lung and MEF mouse embryonic feeder cells. PVDF membrane was probed with 0.1 µg/mL of Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for S100A10 at approximately 11 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

S100A10 was detected in perfusion fixed frozen sections of mouse kidney using Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in epithelial cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Simple Western lane view shows lysates of mouse lung tissue and MEF mouse embryonic feeder cells, loaded at 0.2 mg/mL. A specific band was detected for S100A10 at approximately 11 kDa (as indicated) using 1 µg/mL of Goat Anti-Mouse S100A10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2377) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the12-230 kDa separation system.

• Reactivity: Mu
• Applications: WB, Simple Western, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

S100A10 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant mouse S100A10
  Pro2-Lys97
  Accession # P08207
• Specificity: Detects mouse S100A10 in direct ELISAs and Western blots. In these formats, approximately 5% cross-reactivity with recombinant human (rh) S100A10 is observed and less than 1% cross-reactivity with rhS100B and recombinant mouse S100A9 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: S100A10
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5-15 ug/mL
  • Simple Western 1 ug/mL
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for S100A10 Antibody [Unconjugated]

• annexin II ligand, calpactin I, light polypeptide
• ANX2L
• ANX2LG
• CAL1LGP11,42C
• Calpactin I light chain
• Calpactin-1 light chain
• Cellular ligand of annexin II
• CLP11Ca[1]
• MGC111133
• p10 protein
• p10
• P11
• protein S100-A10
• S100 calcium binding protein A10 (annexin II ligand, calpactin I, lightpolypeptide (p11))
• S100 calcium binding protein A10
• S100 calcium-binding protein A10 (annexin II ligand, calpactin I, lightpolypeptide (p11))
• S100 calcium-binding protein A10
• S100A10

Background

S100A10 is a member of the S100 family of EF hand calcium-binding proteins. It is primarily found in mast cells as well as heterotetrameric complexes (two annexin A2 subunits and two S100 A10 subunits) that are present intracellularly at the plasma membrane or on the extracellular cell surface. Extracellular S100A10 is a key plasminogen receptor that plays an important role in cellular plasmin production and cellular invasiveness.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for S100A10 Antibody (AF2377)(12)

Publications using AF2377

• Y Hwang, HC Kim, EJ Shin Effect of rottlerin on astrocyte phenotype polarization after trimethyltin insult in the dentate gyrus of mice Journal of Neuroinflammation, 2022-06-11;19(1):142. 2022-06-11 [PMID: 35690821] (IHC, Mouse)
• B Hou, Y Zhang, P Liang, Y He, B Peng, W Liu, S Han, J Yin, X He Inhibition of the NLRP3-inflammasome prevents cognitive deficits in experimental autoimmune encephalomyelitis mice via the alteration of astrocyte phenotype Cell Death Dis, 2020-05-15;11(5):377. 2020-05-15 [PMID: 32415059] (Western Blot, Mouse)
• C Zuo, H Cao, F Ding, J Zhao, Y Huang, G Li, S Huang, H Jiang, Y Jiang, F Wang Neuroprotective efficacy of different levels of high-frequency repetitive transcranial magnetic stimulation in mice with CUMS-induced depression: Involvement of the p11/BDNF/Homer1a signaling pathway J Psychiatr Res, 2020-04-05;125(0):152-163. 2020-04-05 [PMID: 32289652] (Western Blot, Mouse)
• J Yamada, C Sato, K Konno, M Watanabe, S Jinno PSA-NCAM colocalized with cholecystokinin-expressing cells in the hippocampus is involved in mediating antidepressant efficacy J. Neurosci., 2019-12-04;0(0):. 2019-12-04 [PMID: 31801810] (IHC, Mouse)
• D Sargin, RU Chottekala, KE Perit, V Yao, D Chu, DW Sparks, S Kalik, SK Power, OG Troyanskay, EF Schmidt, P Greengard, EK Lambe Mapping the physiological and molecular markers of stress and SSRI antidepressant treatment in S100a10 corticostriatal neurons Mol. Psychiatry, 2019-08-20;25(5):1112-1129. 2019-08-20 [PMID: 31431686] (IHC, Mouse)
• G Liu, Y Wang, W Zheng, H Cheng, R Zhou P11 Loss-of-Function is Associated with Decreased Cell Proliferation and Neurobehavioral Disorders in Mice Int. J. Biol. Sci., 2019-05-20;15(7):1383-1395. 2019-05-20 [PMID: 31337969] (Western Blot, Mouse)
• J Jin, DL Bhatti, KW Lee, L Medrihan, J Cheng, J Wei, P Zhong, Z Yan, C Kooiker, C Song, JH Ahn, GJ Obermair, A Lee, J Gresack, P Greengard, Y Kim Ahnak scaffolds p11/Anxa2 complex and L-type voltage-gated calcium channel and modulates depressive behavior Mol. Psychiatry, 2019-02-13;0(0):. 2019-02-13 [PMID: 30760886] (Western Blot, Mouse)
• T Vanmierlo, J De Vry, E Nelissen, A Sierksma, N Roumans, HWM Steinbusch, LP Wennogle, D van den Ho, J Prickaerts Gestational stress in mouse dams negatively affects gestation and postpartum hippocampal BDNF and P11 protein levels Mol. Cell. Neurosci., 2018-02-28;0(0):. 2018-02-28 [PMID: 29501719] (IHC-F, Mouse)
• S Sajgo, MG Ghinia, M Brooks, F Kretschmer, K Chuang, S Hiriyanna, Z Wu, O Popescu, TC Badea Molecular codes for cell type specification in Brn3 retinal ganglion cells Proc. Natl. Acad. Sci. U.S.A., 2017-05-02;0(0):. 2017-05-02 [PMID: 28465430] (IHC, Mouse)
• Schmidt EF, Warner-Schmidt JL, Otopalik BG, Pickett SB, Greengard P, Heintz N Identification of the cortical neurons that mediate antidepressant responses. Cell, 2012-05-25;149(5):1152-63. 2012-05-25 [PMID: 22632977] (IHC, Mouse)
• Hanaue M, Miwa N, Uebi T, Fukuda Y, Katagiri Y, Takamatsu K Characterization of S100A11, a suppressive factor of fertilization, in the mouse female reproductive tract. Mol. Reprod. Dev., 2011-01-20;78(2):91-103. 2011-01-20 [PMID: 21254279] (Western Blot, Mouse)
• Warner-Schmidt JL, Flajolet M, Maller A, Chen EY, Qi H, Svenningsson P, Greengard P Role of p11 in cellular and behavioral effects of 5-HT4 receptor stimulation. J. Neurosci., 2009-02-11;29(6):1937-46. 2009-02-11 [PMID: 19211900] (IHC, Western Blot, Mouse)

Bioinformatics

• Gene Symbol: S100A10
• Uniprot:
  • P08207()