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DGAT2 Antibody [Biotin]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 405
NBP1-71701AF405
Alexa Fluor 488
NBP1-71701AF488
Alexa Fluor 647
NBP1-71701AF647
Alexa Fluor 700
NBP1-71701AF700
DyLight 405
NBP1-71701V
DyLight 488
NBP1-71701G
DyLight 550
NBP1-71701R
DyLight 650
NBP1-71701C
HRP
NBP1-71701H
Unconjugated
NBP1-71701
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DGAT2 Antibody [Biotin] Summary

Immunogen
A synthetic peptide made to an internal region of the human DGAT2 protein (within residues 200-300). [Swiss-Prot Q96PD7]
Localization
Endoplasmic reticulum membrane; Multi-pass membrane protein
Predicted Species
Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DGAT2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
Application Notes
This DGAT2 antibody is useful for Western blot, where a band is seen ~43 kDa.
Readout System

Reactivity Notes

Human and mouse. Immunogen has 100% identity to rat, bovine and porcine, and 88% identity to Xenopus.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for DGAT2 Antibody [Biotin]

  • DGAT2
  • diacylglycerol O-acyltransferase 2
  • diacylglycerol O-acyltransferase homolog 2 (mouse)
  • diacylglycerol O-acyltransferase homolog 2
  • diacylglycerol O-acyltransferase-like protein 2
  • Diglyceride acyltransferase 2
  • DKFZp686A15125
  • EC 2.3.1
  • EC 2.3.1.20
  • GS1999FULL

Background

Diacylglycerol acyltransferase enzyme 2 is localized within the endoplasmic reticulum, mitochondria and lipid droplets. It catalyzes triacylglycerol synthesis, which creates the major form of stored energy in cells. In mice, when DGAT2 is overexpressed in the liver, hepatocytes have abnormally high levels of lipid retention, elevated triglyceride, diacylglycerol and ceramide, while maintaining a normal insulin response. This indicates that insulin resistance and elevated hepatic lipid retention are associated. DGAT2 is associated with increases in PKC activation, which is implicated in the formation of nonalcoholic fatty liver disease. DGAT2 is critical for lipid droplet formation in adipocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [NB110-41487] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti-DGAT1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
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NB400-114
Western Blot: Fatty Acid Synthase Antibody [NB400-114] - Expression of Fatty Acid Synthase in chicken hypothalamus. A: total RNA (1 ug) isolated from different tissues (brain, hypothalamus, liver and muscle) was subjected to RT-PCR using specific primers for chicken Fatty Acid Synthase (GenBank accession JO4485) or ribosomal 18S as a control (GenBank accession AF173612) B: tissue lysates (brain, hypothalmus, liver and muscle) were subjected to Western blot as described in MATERIALS AND METHODS. Blots were incubated with anti-Fatty Acid Synthase antibody and revealed by enhanced chemiluminescence. Picture compliments of Dridi S. et al, Am J Physiol Regul Integr Comp Physiol. 2006 Jul;291(1):R138-47. Epub 2006 Feb 2.
Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase Antibody [NB400-114] - The Fas antibody was tested in MCF-7 cells at a 1:2000 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.
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Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11+6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. Human Thyroid stained with Thyroglobulin Ab (2H11 + 6E1).
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Immunohistochemistry: LRAT Antibody (M34-P1F10) [NBP2-50444]
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(E2-E3 (same as INS04 ...
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NBP2-22106
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NBP1-26393
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NBP1-85691
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MTTP Antibody
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Glutaminyl-peptide
Cyclotransferase/QPCT ...
NBP1-81838
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NBP2-41211
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Protein RBP Antibody ...
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AF6898
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DGAT2 Antibody
[Biotin]
NBP1-71701B
DGAT2 Antibody [Biotin]
Species: Hu, Mu, Rt, Po, Bv
Applications: WB

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Secondary Antibodies

 

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Triacylglycerols (triglycerides) (TGs) are the major storage molecules of metabolic energy and FAs in most living organisms. Excessive accumulation of TGs, however, is associated with human diseases, such as obesity, diabetes mellitus, and steatohepat...  Read full blog post.

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Bioinformatics

Gene Symbol DGAT2
Entrez