SCD-1 Antibody [Unconjugated]

Images

 
Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and SK‑Mel‑28 human malignant melanoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human SCD‑1 Antigen ...read more
Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SCD-1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human SCD‑1 Antigen ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, KO
Clonality
Polyclonal
Host
Sheep
Conjugate
Unconjugated
Concentration
LYOPH

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Catalog# & Formulation Size Price

SCD-1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human SCD-1
Ala141-Gly221
Accession # O00767
Specificity
Detects human SCD-1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human SCD-5 and recombinant mouse SCD-1 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Sheep
Gene
SCD
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5 ug/mL
  • Knockout Validated
Control
SCD-1 Knockout HeLa Cell Lysate
Reviewed Applications
Read 1 Review rated 5
using
AF7550 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SCD-1 Antibody [Unconjugated]

  • FADS5
  • MSTP008
  • SCD1
  • SCD-1
  • SCDOS
  • Stearoyl-CoA desaturase

Background

SCD-1 (Stearoyl-CoA desaturase 1; also Acyl-CoA desaturase, fatty acid desaturase, and Delta-9 desaturase) is a 37-40 kDa member of the fatty acid desaturase family of enzymes. It is an ER-embedded protein that is expressed by multiple cell types, including adipocytes, hepatocytes, macrophages, endothelial and sebaceous gland cells. SCD-1 catalyzes the formation of monounsaturated fatty acids from saturated fatty acids. It does so by generating a double bond between the C9 and C10 carbons of dietary and/or endogenously synthesized fatty acids. This creates either palmitoleic or oleic acid, two fatty acids that are optimally suited for either storage or inclusion into phospholipids. It also removes a potential source of inflammation, as saturated fatty acids are known to activate TLRs with the subsequent onset of inflammation. Human SCD-1 is a 4-transmembrane (TM), 359 amino acid (aa) protein. It contains a 71 aa cytoplasmic N-terminus, followed by two TM segments (aa 72-119) and an extended cytoplasmic region (aa 120-216) that possesses three utilized Ser/Thr phosphorylation sites, two additional TM segments (aa 217-273), and a C-terminal cytoplasmic tail (aa 274-359) that contains most of the catalytic region. There is one potential isoform variant that shows a 13 aa substitution for aa 295-359. Over aa 141-221, human SCD-1 shares 95% aa sequence identity with mouse SCD-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SCD-1 Antibody (AF7550) (0)

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Review for SCD-1 Antibody (AF7550) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF7550:
Filter by Applications
Simple Western
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Simple Western SCD-1 AF7550
Enlarge
5
reviewed by:
Zachary Patinkin
Simple Western Human 06/24/2015
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Summary

ApplicationSimple Western
Sample TestedHuman mesenchymal stem cell whole cell lysate
SpeciesHuman
LotCGOP011506A

Blocking

Blocking DetailsProtein Simple Antibody Diluent, 5 min, 25C

Primary Anitbody

Dilution Ratio10 ug/mL, 30 min, Protein Simple Antibody Diluent

Secondary Antibody

Secondary Descriptionanti-Sheep IgG
Secondary Manufacturer Cat#R&D HAF016
Secondary Concentration1:50

Details

Detection NotesWES multi-image analysis

Comments

CommentsHigher sample concentrations that were tested led to a broad non-specific band at higher molecular weight.

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for SCD-1 Antibody (AF7550) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

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Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Zachary Patinkin
06/24/2015
Application: Simple Western
Species: Human

Bioinformatics

Gene Symbol SCD
Uniprot