SCD-1 Antibody [Unconjugated]

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line and SK‑Mel‑28 human malignant melanoma cell line. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human SCD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7550) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SCD‑1 at approximately 38 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and SCD-1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human SCD‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7550) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for SCD‑1 at approximately 41 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Hu
• Applications: WB, KO
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

SCD-1 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human SCD-1
  Ala141-Gly221
  Accession # O00767
• Specificity: Detects human SCD-1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant human SCD-5 and recombinant mouse SCD-1 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: SCD
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Knockout Validated
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.2 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for SCD-1 Antibody [Unconjugated]

• FADS5
• MSTP008
• SCD1
• SCD-1
• SCDOS
• Stearoyl-CoA desaturase

Background

SCD-1 (Stearoyl-CoA desaturase 1; also Acyl-CoA desaturase, fatty acid desaturase, and Delta-9 desaturase) is a 37-40 kDa member of the fatty acid desaturase family of enzymes. It is an ER-embedded protein that is expressed by multiple cell types, including adipocytes, hepatocytes, macrophages, endothelial and sebaceous gland cells. SCD-1 catalyzes the formation of monounsaturated fatty acids from saturated fatty acids. It does so by generating a double bond between the C9 and C10 carbons of dietary and/or endogenously synthesized fatty acids. This creates either palmitoleic or oleic acid, two fatty acids that are optimally suited for either storage or inclusion into phospholipids. It also removes a potential source of inflammation, as saturated fatty acids are known to activate TLRs with the subsequent onset of inflammation. Human SCD-1 is a 4-transmembrane (TM), 359 amino acid (aa) protein. It contains a 71 aa cytoplasmic N-terminus, followed by two TM segments (aa 72-119) and an extended cytoplasmic region (aa 120-216) that possesses three utilized Ser/Thr phosphorylation sites, two additional TM segments (aa 217-273), and a C-terminal cytoplasmic tail (aa 274-359) that contains most of the catalytic region. There is one potential isoform variant that shows a 13 aa substitution for aa 295-359. Over aa 141-221, human SCD-1 shares 95% aa sequence identity with mouse SCD-1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for SCD-1 Antibody (AF7550) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Simple Western Human 06/24/2015

Summary

• Application: Simple Western
• Sample Tested: Human mesenchymal stem cell whole cell lysate
• Species: Human
• Lot: CGOP011506A

Bioinformatics

• Gene Symbol: SCD
• Uniprot:
  • O00767()