Acetyl-CoA Carboxylase alpha/ACACA Antibody

Images

 
Immunoprecipitation: Acetyl-CoA Carboxylase alpha/ACACA Antibody [NB100-55246] - Detection of Human ACC1/ACC-alpha on HeLa whole cell lysates using NB100-55246. ACC1/ACC-alpha was blotted using rabbit ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IP, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

Acetyl-CoA Carboxylase alpha/ACACA Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1925 and 1975 of human acetyl-Coenzyme A carboxylase alpha using the numbering given in entry NP_942131.1 (GeneID 31).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ACACA
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoprecipitation 2-5 ug/mg lysate

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Acetyl-CoA Carboxylase alpha/ACACA Antibody

  • ACACA
  • ACACacetyl-CoA carboxylase 1
  • ACC1
  • ACC1ACC
  • ACCA
  • ACC-alpha
  • AcetylCoA Carboxylase alpha
  • Acetyl-CoA Carboxylase alpha
  • acetyl-Coenzyme A carboxylase alpha
  • EC 6.4.1.2

Background

In cells, excess metabolic fuel is converted into fatty acids in cytosol and oxidized later in mitochondria to generate ATP and acetyl CoA. In fatty acid synthesis, catalytic formation of malonyl CoA from acetyl CoA by Acetyl CoA carboxylase (ACC1) is the rate limiting step. The translocation of LCFA CoA from cytosol to mitochondria is catalyzed by two carnitine palmitoyl transferases (CPT1 and CPT2) and regulated by ACC2, the rate limiting step of mitochondrial fatty acid beta oxidation. Activities of ACC1 and 2 are regulated by their phosphorylation by 5' AMP activated protein kinase (AMPK). Diabetes deranges AMPK master switch and represses the ACC1 gene expression and stimulates excessive fatty acid oxidation which in turn interferes with glucose metabolism. ACC1 is also known as ACC alpha and is a cytosolic enzyme, enriched in liver, adipose, and lactating mammary tissues. ACC1 catalyzes the carboxylation of acetyl CoA to form malonyl CoA, the rate limiting step in the biogenesis of LCFA CoA. ACC1 has three main functions acting as a biotin carboxyl carrier protein, a biotin carboxylase, and a carboxyltransferase (catalytic activity). Two variants of ACC1 have been described. One with 8 additional amino acids commencing at Pro 1196. The other, which is 59aa shorter than the predominant fat and liver isoform, exists in mammals. The presence of 8 additional amino acids inhibits the in vitro phosphorylation of the Ser1200 by cAMP dependent kinase. The two ACC1 isoforms are differentially regulated in a tissue specific manner and under different physiological conditions. The activity of ACC1 is finely regulated by hormone dependent phosphorylation and dephosphorylation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB400-114
Species: Hu, Mu, Rt, Po, Ch, Ha, Pl, Pm
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-22127
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ELISA, Flow, ICC/IF, IHC, IHC-P
AF2850
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, ICC
NBP1-87487
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-34260
Species: Hu, Mu, Po, Bv, Rb
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP, PAGE
NB120-13550
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-90274
Species: Hu
Applications: WB, IHC, IHC-P
NB600-582
Species: Hu, Mu, Rt, Ca, Ch, SyHa, Ha
Applications: WB, Simple Western
NB100-104
Species: Hu, Mu
Applications: WB, ELISA, Flow, IB, ICC/IF, IHC, IHC-P, IP
NBP2-01833
Species: Hu, Mk
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-22106
Species: Hu, Mu
Applications: WB, IHC, IHC-P
AF7550
Species: Hu
Applications: WB
AF7197
Species: Hu, Mu
Applications: WB, IHC, ICC
NBP1-49533
Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC-P
NBP1-90276
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NB100-55246
Species: Hu
Applications: IP, WB (-)

Publications for Acetyl-CoA Carboxylase alpha/ACACA Antibody (NB100-55246) (0)

There are no publications for Acetyl-CoA Carboxylase alpha/ACACA Antibody (NB100-55246).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Acetyl-CoA Carboxylase alpha/ACACA Antibody (NB100-55246) (0)

There are no reviews for Acetyl-CoA Carboxylase alpha/ACACA Antibody (NB100-55246). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Acetyl-CoA Carboxylase alpha/ACACA Antibody (NB100-55246) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Acetyl-CoA Carboxylase alpha/ACACA Products

Bioinformatics Tool for Acetyl-CoA Carboxylase alpha/ACACA Antibody (NB100-55246)

Discover related pathways, diseases and genes to Acetyl-CoA Carboxylase alpha/ACACA Antibody (NB100-55246). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Acetyl-CoA Carboxylase alpha/ACACA Antibody (NB100-55246)

Discover more about diseases related to Acetyl-CoA Carboxylase alpha/ACACA Antibody (NB100-55246).
 

Pathways for Acetyl-CoA Carboxylase alpha/ACACA Antibody (NB100-55246)

View related products by pathway.

PTMs for Acetyl-CoA Carboxylase alpha/ACACA Antibody (NB100-55246)

Learn more about PTMs related to Acetyl-CoA Carboxylase alpha/ACACA Antibody (NB100-55246).
 

Research Areas for Acetyl-CoA Carboxylase alpha/ACACA Antibody (NB100-55246)

Find related products by research area.

Blogs on Acetyl-CoA Carboxylase alpha/ACACA

There are no specific blogs for Acetyl-CoA Carboxylase alpha/ACACA, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Acetyl-CoA Carboxylase alpha/ACACA Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol ACACA
Entrez
Uniprot