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DGAT2 Antibody [Janelia Fluor® 646]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Rt, Po, BvSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Janelia Fluor 646

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
NBP1-71701AF350
Alexa Fluor 405
NBP1-71701AF405
Alexa Fluor 488
NBP1-71701AF488
Alexa Fluor 532
NBP1-71701AF532
Alexa Fluor 594
NBP1-71701AF594
Alexa Fluor 647
NBP1-71701AF647
Alexa Fluor 700
NBP1-71701AF700
Alexa Fluor 750
NBP1-71701AF750
Biotin
NBP1-71701B
DyLight 350
NBP1-71701UV
DyLight 405
NBP1-71701V
DyLight 488
NBP1-71701G
DyLight 550
NBP1-71701R
DyLight 594
NBP1-71701DL594
DyLight 650
NBP1-71701C
DyLight 680
NBP1-71701FR
DyLight 755
NBP1-71701IR
HRP
NBP1-71701H
Janelia Fluor 549
NBP1-71701JF549
Unconjugated
NBP1-71701
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DGAT2 Antibody [Janelia Fluor® 646] Summary

Immunogen
A synthetic peptide made to an internal region of the human DGAT2 protein (within residues 200-300). [Swiss-Prot Q96PD7]
Localization
Endoplasmic reticulum membrane; Multi-pass membrane protein
Predicted Species
Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DGAT2
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Western Blot
  • Immunohistochemistry
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Human and mouse. Immunogen has 100% identity to rat, bovine and porcine, and 88% identity to Xenopus. Rat reactivity reported in scientific literature (PMID:33129840).

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for DGAT2 Antibody [Janelia Fluor® 646]

  • DGAT2
  • diacylglycerol O-acyltransferase 2
  • diacylglycerol O-acyltransferase homolog 2 (mouse)
  • diacylglycerol O-acyltransferase homolog 2
  • diacylglycerol O-acyltransferase-like protein 2
  • Diglyceride acyltransferase 2
  • DKFZp686A15125
  • EC 2.3.1
  • EC 2.3.1.20
  • GS1999FULL

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: DGAT1 Antibody [NB110-41487] - Detection of DGAT1 in HepG2 lysate.
Immunocytochemistry/Immunofluorescence: DGAT1 Antibody [NB110-41487] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were incubated with anti-DGAT1 at 5 ug/ml overnight at 4C and detected with an anti-rabbit DyLight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Western Blot: DGAT1 Antibody [NB110-41487] - Factors regulating lipid storage and breakdown in C2C12 muscle cells.Muscle cells were incubated with PALM (open), NORM (filled), or HSFA (hatched). Pictured is protein abundance of DGAT1. In the figure panel, the data is expressed relative to a no fatty acid control condition (0mM). In panel B, #P<0.05 for a main effect of HSFA vs. PALM and NORM. Representative blot shown inset above the figure panel. DGAT = diacylglycerol acyltransferase; AU = arbitrary units. Image collected and cropped by CiteAb from the following publication (http://dx.plos.org/10.1371/journal.pone.0120871), licensed under a CC-BY licence.
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Species: Hu, Mu, Rt
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Fatty Acid
Synthase/FASN Antibod
NB400-114
Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Expression of Fatty Acid Synthase in chicken hypothalamus. A: total RNA (1 ug) isolated from different tissues (brain, hypothalamus, liver and muscle) was subjected to RT-PCR using specific primers for chicken Fatty Acid Synthase (GenBank accession JO4485) or ribosomal 18S as a control (GenBank accession AF173612) B: tissue lysates (brain, hypothalmus, liver and muscle) were subjected to Western blot as described in MATERIALS AND METHODS. Blots were incubated with anti-Fatty Acid Synthase antibody and revealed by enhanced chemiluminescence. Picture compliments of Dridi S. et al, Am J Physiol Regul Integr Comp Physiol. 2006 Jul;291(1):R138-47. Epub 2006 Feb 2.
Immunocytochemistry/Immunofluorescence: Fatty Acid Synthase/FASN Antibody [NB400-114] - The Fas antibody was tested in MCF-7 cells at a 1:2000 dilution against Dylight 488 (Green). Alpha tubulin and nuclei were counterstained against Dylight 550 (Red) and DAPI (Blue), respectively.
Immunohistochemistry-Paraffin: Fatty Acid Synthase/FASN Antibody [NB400-114] - Immunohistochemical localization of FAS in chicken hypothalmus. Paraffin sections were obtained from 3-wk-old broiler chickens.
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Thyroglobulin
Antibody (2H11 + 6E1)
NBP2-34294
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Thyroglobulin staining in thyroid tissue of normal C57 mouse (Red). Dilution is 1:100. This image was submitted via customer Review.
Immunohistochemistry-Paraffin: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Analysis using Azide/BSA FREE version of NBP2-34294. Human Thyroid stained with Thyroglobulin Ab (2H11 + 6E1).
Immunohistochemistry: Thyroglobulin Antibody (2H11 + 6E1) [NBP2-34294] - Imaging of Mouse stomach tissue. Positive staining can only be observed inside the thyroid cells and the follicle in thyroid tissue. This image was submitted via customer Review.
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Applications: WB, Flow, IHC, IHC-P
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(M34-P1F10)
NBP2-50444
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Immunocytochemistry/Immunofluorescence: LRAT Antibody (M34-P1F10) [NBP2-50444]
Immunohistochemistry: LRAT Antibody (M34-P1F10) [NBP2-50444]
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Insulin Antibody
(E2-E3 (same as INS04 ...
NBP2-34260
Western Blot: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Probing of Western blots of pancreatic lysates from control and iron overloaded Tg40 PrP and PrP-/- mice with antibodies specific for insulin dimer and pentamer (upper and lower panels) shows decreased expression in Tg40 PrP relative to PrP-/- samples (lanes 1 & 5 vs. 3 & 7). Image collected and cropped by CiteAb from the following publication (nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
Immunohistochemistry: Insulin Antibody (E2-E3 (same as INS04)) [NBP2-34260] - Immunohistochemistry of pancreas shows relatively higher reactivity for insulin in PrP-/- relative to Tg40 PrP sections (panels 1 & 3). Iron overloading decreases insulin reactivity in Tg40 PrP but not in PrP-/- samples (panels 1 vs. 2 & 3 vs. 4). Reaction for glucagon is higher in iron overloaded Tg40 PrP relative to control samples (panels 5 & 6). The difference is minimal in PrP-/- samples (panels 7 & 8). Image collected and cropped by CiteAb from the following publication (http://www.nature.com/articles/s41598-018-24786-1), licensed under a CC-BY licence.
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(1 Publication)
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SREBP1 Antibody
(2A4)
NB600-582
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PPAR gamma/NR1C3
Antibody
NBP2-22106
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[Unconjugated]
AF7550
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NBP1-85691
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NBP1-62489
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Glutaminyl-peptide
Cyclotransferase/QPCT ...
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Western Blot: Glutaminyl-peptide Cyclotransferase/QPCT Antibody [NBP1-81838] - Analysis in human cell line SK-MEL-30.
(1 Publication)
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GRIP1 Antibody
NBP2-41211
Western Blot: GRIP1 Antibody [NBP2-41211] - Analysis of GRIP1 in 293 cell lysate with GRIP1 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.
Immunohistochemistry: GRIP1 Antibody [NBP2-41211] - Immunohistochemistry of GRIP1 in rat brain tissue with GRIP1 antibody at 2.5 ug/ml.
Immunofluorescence: GRIP1 Antibody [NBP2-41211] - Immunofluorescence of GRIP1 in rat brain tissue with GRIP1 antibody at 20 ug/ml.
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Fas/TNFRSF6/CD95
[HRP]
DFS00
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RBP2 Antibody
NBP1-85464
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Immunocytochemistry/Immunofluorescence: RBP2 Antibody [NBP1-85464] - Immunofluorescent staining of human cell line CACO-2 shows localization to the Golgi apparatus.
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Retinol Binding
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NBP2-00637
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Acetyl-CoA
Carboxylase alpha/ACA ...
NBP2-89114
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Immunohistochemistry-Paraffin: Acetyl-CoA Carboxylase alpha/ACACA Antibody [NBP2-89114] - Analysis of paraffin-embedded human kidney tissue using anti-Acetyl CoA Carboxylase 1 (ACC1) antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.
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Species: Hu, Mu, Rt
Applications: WB, Flow, ICC/IF, IHC, IHC-P
DGAT2 Antibody
[Janelia Fluor® ...
NBP1-71701JF646
DGAT2 Antibody [Janelia Fluor® 646]
Species: Hu, Mu, Rt, Rt, Po, Bv
Applications: WB, IHC

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP1-71701AF350
Alexa Fluor 405 NBP1-71701AF405
Alexa Fluor 488 NBP1-71701AF488
Alexa Fluor 532 NBP1-71701AF532
Alexa Fluor 594 NBP1-71701AF594
Alexa Fluor 647 NBP1-71701AF647
Alexa Fluor 700 NBP1-71701AF700
Alexa Fluor 750 NBP1-71701AF750
Biotin NBP1-71701B
DyLight 350 NBP1-71701UV
DyLight 405 NBP1-71701V
DyLight 488 NBP1-71701G
DyLight 550 NBP1-71701R
DyLight 594 NBP1-71701DL594
DyLight 650 NBP1-71701C
DyLight 680 NBP1-71701FR
DyLight 755 NBP1-71701IR
HRP NBP1-71701H
Janelia Fluor 549 NBP1-71701JF549
Janelia Fluor 646 NBP1-71701JF646
Learn about Custom Antibody Labeling

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Diseases for DGAT2 Antibody (NBP1-71701JF646)

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Blogs on DGAT2.

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Can you DGAT? DGAT1 & DGAT2 Antibodies for Metabolic Pathway Research
Triacylglycerols (triglycerides) (TGs) are the major storage molecules of metabolic energy and FAs in most living organisms. Excessive accumulation of TGs, however, is associated with human diseases, such as obesity, diabetes mellitus, and steatohepat...  Read full blog post.
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Western Blot: Fatty Acid Synthase/FASN Antibody [NB400-114] - Expression of Fatty Acid Synthase in chicken hypothalamus. A: total RNA (1 ug) isolated from different tissues (brain, hypothalamus, liver and muscle) was subjected to RT-PCR using specific primers for chicken Fatty Acid Synthase (GenBank accession JO4485) or ribosomal 18S as a control (GenBank accession AF173612) B: tissue lysates (brain, hypothalmus, liver and muscle) were subjected to Western blot as described in MATERIALS AND METHODS. Blots were incubated with anti-Fatty Acid Synthase antibody and revealed by enhanced chemiluminescence. Picture compliments of Dridi S. et al, Am J Physiol Regul Integr Comp Physiol. 2006 Jul;291(1):R138-47. Epub 2006 Feb 2.
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Bioinformatics

Gene Symbol DGAT2