DGAT1 Antibody

Images

 
Western Blot: DGAT1 Antibody [NB110-41487] - Detection of DGAT1 in HepG2 lysate.
Immunocytochemistry/ Immunofluorescence: DGAT1 Antibody [NB110-41487] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ze, Gt, Pm, ShSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

DGAT1 Antibody Summary

Immunogen
A synthetic peptide made to an internal region (within residues 200-300) of human DGAT1. [Swiss-Prot# O75907]
Localization
Endoplasmic reticulum; endoplasmic reticulum membrane multi-pass membrane protien.
Predicted Species
Primate (100%), Sheep (100%), Goat (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DGAT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:40-1:100
Application Notes
This DGAT1 antibody is useful for Western Blot analysis and Immunocytochemistry/Immunofluorescence. In Western Blot, a band is seen at ~55 kDa. In ICC/IF, staining was observed in the endoplasmic reticulum and cytoplasm of HepG2 cells. This antibody is not applicable for IHC-paraffin embedded sections. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NB110-41487 in the following applications:

Publications
Read Publications using
NB110-41487 in the following applications:

  • WB
    7 publications

Reactivity Notes

Predicted to react with primate, sheep, and goat based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DGAT1 Antibody

  • ACAT-related gene product 1
  • AGRP1
  • ARGP1acyl coenzyme A:cholesterol acyltransferase related gene 1
  • DGATACAT related gene product 1
  • diacylglycerol O-acyltransferase 1
  • diacylglycerol O-acyltransferase homolog 1 (mouse)
  • diacylglycerol O-acyltransferase homolog 1
  • Diglyceride acyltransferase
  • EC 2.3.1
  • EC 2.3.1.20

Background

Anabolic signals such as glucose, insulin etc. enhance the transcription of lipogenic enzymes responsible for converting excessive carbohydrates into triglycerides, whereas, DGAT1 (diacylglycerol O-acyltransferase 1) is an enzyme that facilitates the conversion of fatty acids to triglycerides. DGAT1 is a multi-pass membrane protein localized in endoplasmic reticulum which catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2, it is not essential for survival, and is involved in VLDL assembly. In liver, DGAT1 plays a key role in esterifying exogenous fatty acids to glycerol, and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation. Moreover, it acts as the major acyl-CoA retinol acyltransferase (ARAT) in skin tissues, wherein, it maintains the retinoid homeostasis for preventing retinoid toxicity responsible for skin and hair disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DGAT1 Antibody (NB110-41487)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


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WB
(7)
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Human
(4)
Mouse
(4)
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Showing Publications 1 - 9 of 9.
Publications using NB110-41487 Applications Species
Van Pelt DW, Guth LM, Wang AY, Horowitz JF. Factors regulating subcutaneous adipose tissue storage, fibrosis, and inflammation may underlie low fatty acid mobilization in insulin sensitive obese adults Am. J. Physiol. Endocrinol. Metab. 2017 Jul 05 [PMID: 28679624] (WB, Human) WB Human
Cubizolle A, Guillou L, Mollereau B et al. Fatty acid transport protein 1 regulates retinoid metabolism and photoreceptor development in mouse retina PLoS ONE 2017 Jul 03 [PMID: 28672005] (WB, Mouse) WB Mouse
Newsom SA, Everett AC, Park S et al. Lipid mixtures containing a very high proportion of saturated Fatty acids only modestly impair insulin signaling in cultured muscle cells. PLoS ONE. 2015 Mar 21 [PMID: 25793412] (WB, Mouse) WB Mouse
Choi YJ, Lee CH, Lee KY et al. Increased Hepatic Fatty Acid Uptake and Esterification Contribute to Tetracycline-Induced Steatosis in Mice Toxicol. Sci. 2015 Mar 04 [PMID: 25745068] (WB, Human, Mouse)

Details:
DGAT1 antibody used for WB on lysates of liver tissues from mice administered or not with Tetracycline (FIG. 2A), whole cell lysates of HepG2 cells treated or not with Tetracycline, U0126 or PD98059 (FIG. 3C, 5A/B, Supplementary Figure 2B). Antibody also used for WB on lysates of HepG2 cells transfected with mock or HA tagged CA-MEK plasmid as well as on HepG2 cells subjected (FIG. 5A/B) or not to si-RNA mediated double knockdown of DGAT1/2 (FIG. 6A).
WB Human, Mouse
Larsen S, Danielsen JH, Sondergard SD et al. The effect of high-intensity training on mitochondrial fat oxidation in skeletal muscle and subcutaneous adipose tissue. Scand J Med Sci Sports. 2014 May 21 [PMID: 24845952] (WB, Human) WB Human
Cornford A, Hinko A, Nelson R et al. Rapid development of systemic insulin resistance with overeating is not accompanied by robust changes in skeletal muscle glucose and lipid metabolism. Appl Physiol Nutr Metab. 2013 [PMID: 23668758]
Haas JT, Winter HS, Lim E et al. DGAT1 mutation is linked to a congenital diarrheal disorder J Clin Invest 2012 Dec 3 [PMID: 23114594] (WB, Mouse) WB Mouse
Li M, Paran C, Wolins NE, Horowitz JF. High muscle lipid content in obesity is not due to enhanced activation of key triglyceride esterification enzymes or to the suppression of lipolytic proteins. Am J Physiol Endocrinol Metab. 2011 Feb 1. [PMID: 21285405]
Newsom SA, Schenk S, Li M et al. High fatty acid availability after exercise alters the regulation of muscle lipid metabolism. Metabolism. 2010 Sep 24. [PMID: 20870251] (WB, Human) WB Human

Review for DGAT1 Antibody (NB110-41487) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB110-41487:
Filter by Applications
Simple Western
(1)
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Mouse
(1)
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Images Ratings Applications Species Date Details
Simple Western DGAT1 NB110-41487
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reviewed by:
Zachary Patinkin
Simple Western Mouse 07/22/2015
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Summary

ApplicationSimple Western
Sample TestedMouse skeletal muscle homogenate
SpeciesMouse
LotF-1

Blocking

Blocking DetailsProtein Simple Antibody Diluent, 5min, 25C

Primary Anitbody

Dilution Ratio1:25, 30 min, Protein Simple Antibody Diluent

Secondary Antibody

Secondary DescriptionAnti-rabbit secondary antibody
Secondary Manufacturer Cat#Protein Simple 042-206
Secondary Concentrationundiluted

Details

Detection NotesWES multi-image analysis

Product General Protocols

View specific protocols for DGAT1 Antibody (NB110-41487): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DGAT1 Antibody (NB110-41487). (Showing 1 - 2 of 2 FAQs).

  1. I would like to order your anti-DGAT1 antibody NB110-41487. The immunogen for this is a synthetic peptide designed within residues 200-300 of human DGAT1. I would like to use it to detect the homolog of DGAT1 in Drosophila. I know that the sequence is proprietary but could you tell me if the sequence is conserved in Drosophila (that means that the peptide is designed within residues 243-288 of human DGAT1) so that it could work in flies.
    • Unfortunately, the Drosophila and human DGAT1 sequences are too divergent to recommend that NB110-41487 may cross-react with fly.
  2. Do you offer the blocking peptide for NB110-41487?
    • Unfortunately, we do not offer the blocking peptide for NB110-41487. I apologize for any inconvenience.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-41487AF405
Alexa Fluor 488 Labeled NB110-41487AF488
Alexa Fluor 647 Labeled NB110-41487AF647
Alexa Fluor 700 Labeled NB110-41487AF700
Biotin Labeled NB110-41487B
DyLight 405 Labeled NB110-41487V
DyLight 488 Labeled NB110-41487G
DyLight 550 Labeled NB110-41487R
DyLight 650 Labeled NB110-41487C
FITC Labeled NB110-41487F
HRP Labeled NB110-41487H

Additional Array Products

Array NB110-41487

Bioinformatics Tool for DGAT1 Antibody (NB110-41487)

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Diseases for DGAT1 Antibody (NB110-41487)

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Pathways for DGAT1 Antibody (NB110-41487)

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PTMs for DGAT1 Antibody (NB110-41487)

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Research Areas for DGAT1 Antibody (NB110-41487)

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Blogs on DGAT1.

Can you DGAT? DGAT1 & DGAT2 Antibodies for Metabolic Pathway Research
Triacylglycerols (triglycerides) (TGs) are the major storage molecules of metabolic energy and FAs in most living organisms. Excessive accumulation of TGs, however, is associated with human diseases, such as obesity, diabetes mellitus, and steatohepat...  Read full blog post.

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Recent Reviews

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Zachary Patinkin
07/22/2015
Application: Simple Western
Species: Mouse

Bioinformatics

Gene Symbol DGAT1