DGAT1 Antibody - BSA Free

Images

 
Western Blot: DGAT1 Antibody [NB110-41487] - Detection of DGAT1 in HepG2 lysate.
Immunocytochemistry/ Immunofluorescence: DGAT1 Antibody [NB110-41487] - HepG2 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton X-100. The cells were ...read more
Western Blot: DGAT1 Antibody [NB110-41487] - Factors regulating lipid storage and breakdown in C2C12 muscle cells.Muscle cells were incubated with PALM (open), NORM (filled), or HSFA (hatched). Pictured is protein ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, PmSpecies Glossary
Applications WB, IB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

DGAT1 Antibody - BSA Free Summary

Immunogen
A synthetic peptide made to an internal region (within residues 200-300) of human DGAT1. [Swiss-Prot# O75907]
Localization
Endoplasmic reticulum; endoplasmic reticulum membrane multi-pass membrane protien.
Predicted Species
Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DGAT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunoblotting
  • Immunocytochemistry/Immunofluorescence 1:40-1:100
  • Western Blot 2 ug/ml
Application Notes
This DGAT1 antibody is useful for Western Blot analysis and Immunocytochemistry/Immunofluorescence. In Western Blot, a band is seen at ~55 kDa. In ICC/IF, staining was observed in the endoplasmic reticulum and cytoplasm of HepG2 cells. This antibody is not applicable for IHC-paraffin embedded sections. Use in immunoblotting reported in scientific literature (PMID: 23668758).
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 3
using
NB110-41487 in the following applications:

Publications
Read Publications using
NB110-41487 in the following applications:

Reactivity Notes

Use in Rat reported in scientific literature (PMID:32326330).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DGAT1 Antibody - BSA Free

  • ACAT-related gene product 1
  • AGRP1
  • ARAT
  • ARGP1
  • ARGP1acyl coenzyme A:cholesterol acyltransferase related gene 1
  • DGAT
  • DGAT1
  • DGATACAT related gene product 1
  • diacylglycerol O-acyltransferase 1
  • diacylglycerol O-acyltransferase homolog 1 (mouse)
  • diacylglycerol O-acyltransferase homolog 1
  • DIAR7
  • Diglyceride acyltransferase
  • EC 2.3.1
  • EC 2.3.1.20

Background

Anabolic signals such as glucose, insulin etc. enhance the transcription of lipogenic enzymes responsible for converting excessive carbohydrates into triglycerides, whereas, DGAT1 (diacylglycerol O-acyltransferase 1) is an enzyme that facilitates the conversion of fatty acids to triglycerides. DGAT1 is a multi-pass membrane protein localized in endoplasmic reticulum which catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol and fatty acyl CoA as substrates. In contrast to DGAT2, it is not essential for survival, and is involved in VLDL assembly. In liver, DGAT1 plays a key role in esterifying exogenous fatty acids to glycerol, and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation. Moreover, it acts as the major acyl-CoA retinol acyltransferase (ARAT) in skin tissues, wherein, it maintains the retinoid homeostasis for preventing retinoid toxicity responsible for skin and hair disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB, IB, ICC/IF

Publications for DGAT1 Antibody (NB110-41487)(20)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.

We have publications tested in 2 applications: Inhibition Activity, WB.


Filter By Application
Inhibition Activity
(1)
WB
(15)
All Applications
Filter By Species
Human
(5)
Mouse
(8)
Rat
(3)
All Species
Showing Publications 1 - 10 of 20. Show All 20 Publications.
Publications using NB110-41487 Applications Species
Ahn C, Ryan BJ, Schleh MW et al. Exercise training remodels subcutaneous adipose tissue in adults with obesity even without weight loss The Journal of physiology Mar 6 2022 [PMID: 35249225]
Haberl EM, Pohl R, Rein-Fischboeck L Et al. Accumulation of cholesterol, triglycerides and ceramides in hepatocellular carcinomas of diethylnitrosamine injected mice Lipids in health and disease Oct 10 2021 [PMID: 34629057] (WB, Mouse) WB Mouse
Oke S, Lee K, Papp R Et Al. In Utero Exposure to 9-Tetrahydrocannabinol Leads to Postnatal Catch-up Growth and Dysmetabolism in the Adult Rat Liver preprints Jun 30 2021 [PMID: 34299119] (WB) WB
Romano A, Friuli M, Del Coco L, et al. Chronic Oleoylethanolamide Treatment Decreases Hepatic Triacylglycerol Level in Rat Liver by a PPAR gamma /SREBP-Mediated Suppression of Fatty Acid and Triacylglycerol Synthesis Nutrients Jan 27 2021 [PMID: 33513874] (WB, Rat, Mouse) WB Rat, Mouse
Soltysik K, Ohsaki Y, Tatematsu T et al. Nuclear lipid droplets form in the inner nuclear membrane in a seipin-independent manner The Journal of cell biology Jan 4 2021 [PMID: 33315072]
Haberl EM, Pohl R, Rein-Fischboeck L et al. Hepatic lipid profile in mice fed a choline-deficient, low-methionine diet resembles human non-alcoholic fatty liver disease Lipids in health and disease Dec 9 2020 [PMID: 33298075] (WB, Mouse) WB Mouse
Zhang L, Zhang Z, Li C et al. S100A11 Promotes Liver Steatosis via FOXO1-Mediated Autophagy and Lipogenesis Cell Mol Gastroenterol Hepatol Oct 16 2020 [PMID: 33075563] (WB, Mouse) WB Mouse
Van Pelt D. Effects of Subcutaneous Adipose Tissue on Metabolic Health in Obesity. Thesis. Jul 9 1905 (Inhibition Activity) Inhibition Activity
Harasim-Symbor E, Polak-Iwaniuk A, Konstantynowicz-Nowicka K et al. Experimental Activation of Endocannabinoid System Reveals Antilipotoxic Effects on Cardiac Myocytes Molecules Apr 21 2020 [PMID: 32326330] (WB, Rat) WB Rat
Larsen Steen, Vigelso Andreas, Dandanell Sune et al. Simvastatin-Induced Insulin Resistance May Be Linked to Decreased Lipid Uptake and Lipid Synthesis in Human Skeletal Muscle: the LIFESTAT Study. Journal of Diabetes Research 2018 Sep 12 [PMID: 30276217] (WB, Human) WB Human
Show All 20 Publications.

Review for DGAT1 Antibody (NB110-41487) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB110-41487:
Filter by Applications
Simple Western
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Simple Western DGAT1 NB110-41487
Enlarge
3
reviewed by:
Verified Customer
Simple Western Mouse 07/22/2015
View

Summary

ApplicationSimple Western
Sample TestedMouse skeletal muscle homogenate
SpeciesMouse
LotF-1

Product General Protocols

View specific protocols for DGAT1 Antibody (NB110-41487): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DGAT1 Antibody (NB110-41487). (Showing 1 - 3 of 3 FAQs).

  1. I would like to order your anti-DGAT1 antibody NB110-41487. The immunogen for this is a synthetic peptide designed within residues 200-300 of human DGAT1. I would like to use it to detect the homolog of DGAT1 in Drosophila. I know that the sequence is proprietary but could you tell me if the sequence is conserved in Drosophila (that means that the peptide is designed within residues 243-288 of human DGAT1) so that it could work in flies.
    • Unfortunately, the Drosophila and human DGAT1 sequences are too divergent to recommend that NB110-41487 may cross-react with fly.
  2. Do you offer the blocking peptide for NB110-41487?
    • Unfortunately, we do not offer the blocking peptide for NB110-41487. I apologize for any inconvenience.
  3. Have you ever tested if DGAT1 NB110-41487 work for rat liver tissues?
    • We have not specifically tested rat liver tissue, but rat is a species covered by our guarantee for this product.

Secondary Antibodies

 

Isotype Controls

Additional DGAT1 Products

Array NB110-41487

Bioinformatics Tool for DGAT1 Antibody (NB110-41487)

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Diseases for DGAT1 Antibody (NB110-41487)

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Pathways for DGAT1 Antibody (NB110-41487)

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Blogs on DGAT1.

Can you DGAT? DGAT1 & DGAT2 Antibodies for Metabolic Pathway Research
Triacylglycerols (triglycerides) (TGs) are the major storage molecules of metabolic energy and FAs in most living organisms. Excessive accumulation of TGs, however, is associated with human diseases, such as obesity, diabetes mellitus, and steatohepat...  Read full blog post.

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Verified Customer
07/22/2015
Application: Simple Western
Species: Mouse

Bioinformatics

Gene Symbol DGAT1