DGAT2 Antibody

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Western Blot: DGAT2 Antibody [NBP1-71701] - Analysis of DGAT2 in A. human liver lysate and B. mouse brain lysate.

Product Details

Summary
Reactivity Hu, Mu, Rt, Po, BvSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

DGAT2 Antibody Summary

Immunogen
A synthetic peptide made to an internal region of the human DGAT2 protein (within residues 200-300). [Swiss-Prot Q96PD7]
Localization
Endoplasmic reticulum membrane; Multi-pass membrane protein
Predicted Species
Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DGAT2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
Application Notes
This DGAT2 antibody is useful for Western blot, where a band is seen ~43 kDa.
Positive Control
ACOT7 Antibody (H00011332-B01P)
Publications
Read Publications using
NBP1-71701 in the following applications:

  • WB
    1 publication

Reactivity Notes

Human and mouse. Immunogen has 100% identity to rat, bovine and porcine, and 88% identity to Xenopus.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DGAT2 Antibody

  • DGAT2
  • diacylglycerol O-acyltransferase 2
  • diacylglycerol O-acyltransferase homolog 2 (mouse)
  • diacylglycerol O-acyltransferase homolog 2
  • diacylglycerol O-acyltransferase-like protein 2
  • Diglyceride acyltransferase 2
  • DKFZp686A15125
  • EC 2.3.1
  • EC 2.3.1.20
  • GS1999FULL

Background

Diacylglycerol acyltransferase enzyme 2 is localized within the endoplasmic reticulum, mitochondria and lipid droplets. It catalyzes triacylglycerol synthesis, which creates the major form of stored energy in cells. In mice, when DGAT2 is overexpressed in the liver, hepatocytes have abnormally high levels of lipid retention, elevated triglyceride, diacylglycerol and ceramide, while maintaining a normal insulin response. This indicates that insulin resistance and elevated hepatic lipid retention are associated. DGAT2 is associated with increases in PKC activation, which is implicated in the formation of nonalcoholic fatty liver disease. DGAT2 is critical for lipid droplet formation in adipocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Applications: WB

Publications for DGAT2 Antibody (NBP1-71701)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: WB.


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WB
(1)
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Human
(1)
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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DGAT2 Antibody (NBP1-71701) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-71701AF405
Alexa Fluor 488 Labeled NBP1-71701AF488
Alexa Fluor 647 Labeled NBP1-71701AF647
Alexa Fluor 700 Labeled NBP1-71701AF700
Biotin Labeled NBP1-71701B
DyLight 405 Labeled NBP1-71701V
DyLight 488 Labeled NBP1-71701G
DyLight 550 Labeled NBP1-71701R
DyLight 650 Labeled NBP1-71701C
HRP Labeled NBP1-71701H

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Bioinformatics Tool for DGAT2 Antibody (NBP1-71701)

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Blogs on DGAT2.

Can you DGAT? DGAT1 & DGAT2 Antibodies for Metabolic Pathway Research
Triacylglycerols (triglycerides) (TGs) are the major storage molecules of metabolic energy and FAs in most living organisms. Excessive accumulation of TGs, however, is associated with human diseases, such as obesity, diabetes mellitus, and steatohepat...  Read full blog post.

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Bioinformatics

Gene Symbol DGAT2
Entrez
Uniprot