DGAT2 Antibody - BSA Free

Western Blot: DGAT2 Antibody [NBP1-71701] - Analysis of DGAT2 in A. human liver lysate and B. mouse brain lysate.

• Reactivity: Hu, Mu, Rt, Po, Bv
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

DGAT2 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DGAT2 Antibody - BSA Free (NBP1-71701) is a polyclonal antibody validated for use in WB. Anti-DGAT2 Antibody: Cited in 9 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide made to an internal region of the human DGAT2 protein (within residues 200-300). [Swiss-Prot Q96PD7]
• Localization: Endoplasmic reticulum membrane; Multi-pass membrane protein
• Predicted Species: Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DGAT2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/ml
• Control:

  ACOT7 Antibody - Azide and BSA Free

Reactivity Notes

Immunogen has 100% identity to bovine and porcine, and 88% identity to Xenopus. Rat reactivity reported in scientific literature (PMID:33129840).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DGAT2 Antibody - BSA Free

• DGAT2
• diacylglycerol O-acyltransferase 2
• diacylglycerol O-acyltransferase homolog 2 (mouse)
• diacylglycerol O-acyltransferase homolog 2
• diacylglycerol O-acyltransferase-like protein 2
• Diglyceride acyltransferase 2
• DKFZp686A15125
• EC 2.3.1
• EC 2.3.1.20
• GS1999FULL

Background

Diacylglycerol acyltransferase enzyme 2 is localized within the endoplasmic reticulum, mitochondria and lipid droplets. It catalyzes triacylglycerol synthesis, which creates the major form of stored energy in cells. In mice, when DGAT2 is overexpressed in the liver, hepatocytes have abnormally high levels of lipid retention, elevated triglyceride, diacylglycerol and ceramide, while maintaining a normal insulin response. This indicates that insulin resistance and elevated hepatic lipid retention are associated. DGAT2 is associated with increases in PKC activation, which is implicated in the formation of nonalcoholic fatty liver disease. DGAT2 is critical for lipid droplet formation in adipocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DGAT2 Antibody (NBP1-71701)(9)

Publications using NBP1-71701

• Moliterni C, Vari F, Schifano E et Al. Lipotoxicity of palmitic acid is associated with DGAT1 downregulation and abolished by PPAR? activation in liver cells J Lipid Res 2024-11-04 [PMID: 39505261]
• Eui-Jin Lee, Yeon-Pyo Hong, Yun-Jung Yang Short-term exposure to di(2-ethylhexyl)phthalate may disrupt hepatic lipid metabolism through modulating the oxidative stress in male adolescent rats Environmental Analysis, Health and Toxicology 2024-03-01 [PMID: 38631399]
• S T, S L, F G et al. An aqueous olive leaf extract (OLE) ameliorates parameters of oxidative stress associated with lipid accumulation and induces lipophagy in human hepatic cells Food & function 2023-06-19 [PMID: 37294271] (WB, Human)
  
  Details:
  1:1000 dilution
• Yamasaki S, Kimura G, Koizumi K et al. Maternal green tea extract intake during lactation attenuates hepatic lipid accumulation in adult male rats exposed to a continuous high-fat diet from the foetal period Food & Nutrition Research 2020-10-05 [PMID: 34908919]
• Kim JH, Nagappan A, Jung DY Et al. Histone Demethylase KDM7A Contributes to the Development of Hepatic Steatosis by Targeting Diacylglycerol Acyltransferase 2 International journal of molecular sciences 2021-10-14 [PMID: 34681759] (WB, Mouse)
• Romano A, Friuli M, Del Coco L, et al. Chronic Oleoylethanolamide Treatment Decreases Hepatic Triacylglycerol Level in Rat Liver by a PPAR gamma /SREBP-Mediated Suppression of Fatty Acid and Triacylglycerol Synthesis Nutrients 2021-01-27 [PMID: 33513874]
• Zhang J, Novakovic N, Hua Y et al. Role of lipocalin-2 in extracellular peroxiredoxin 2-induced brain swelling, inflammation and neuronal death Experimental Neurology [PMID: 33129840] (WB, Rat)
• Paland N, Gamliel-Lazarovich A, Coleman R, Fuhrman B. Urokinase-type plasminogen activator (uPA) stimulates triglyceride synthesis in Huh7 hepatoma cells via p38-dependent upregulation of DGAT2. Atherosclerosis. 2014-09-09 [PMID: 25244504] (WB, Human)
• Yang A, Gyulay G, Mitchell M et al. Hypomorphic sialidase expression decreases serum cholesterol by downregulation of VLDL production in mice J Lipid Res 2012-12-01 [PMID: 22984145]

Bioinformatics

• Gene Symbol: DGAT2
• Entrez:
  • 84649(Human)
  • 67800(Mouse)
• Uniprot:
  • Q96PD7(Human)
  • Q9DCV3(Mouse)