DGAT1 Antibody [DyLight 550]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ze, Gt, Pm, ShSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
DyLight 550

DGAT1 Antibody [DyLight 550] Summary

Immunogen
A synthetic peptide made to an internal region (within residues 200-300) of human DGAT1. [Swiss-Prot# O75907]
Localization
Endoplasmic reticulum; endoplasmic reticulum membrane multi-pass membrane protien.
Predicted Species
Primate (100%), Goat (100%), Sheep (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DGAT1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Immunocytochemistry/Immunofluorescence
Application Notes
This DGAT1 antibody is useful for Western Blot analysis and Immunocytochemistry/Immunofluorescence. In Western Blot, a band is seen at ~55 kDa. In ICC/IF, staining was observed in the endoplasmic reticulum and cytoplasm of HepG2 cells. This antibody is not applicable for IHC-paraffin embedded sections.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Predicted to react with primate, sheep, and goat based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
50mM Sodium Borate
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

Dylight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for DGAT1 Antibody [DyLight 550]

  • ACAT-related gene product 1
  • AGRP1
  • ARGP1acyl coenzyme A:cholesterol acyltransferase related gene 1
  • DGATACAT related gene product 1
  • diacylglycerol O-acyltransferase 1
  • diacylglycerol O-acyltransferase homolog 1 (mouse)
  • diacylglycerol O-acyltransferase homolog 1
  • Diglyceride acyltransferase
  • EC 2.3.1
  • EC 2.3.1.20

Background

Glucose and insulin are anabolic signals which upregulate the transcriptions of a series of lipogenic enzymes to convert excess carbohydrate into triglycerides for efficient energy storage. DGAT1 is a microsomal enzyme that assists in the synthesis of fatty acids into triglycerides. DGAT catalyzes the terminal and only committed step in triacylglycerol synthesis by using diacylglycerol (DAG) and fatty acyl CoA as substrates. DGAT plays a fundamental role in the metabolism of cellular diacylglycerol and is important in higher eukaryotes for physiologic processes involving triacylglycerol metabolism such as intestinal fat absorption, lipoprotein assembly, adipose tissue formation, and lactation. DGAT1 is involved in fat absorption in the intestine and in basal level triglyceride synthesis in adipose tissue, where it is more highly expressed.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DGAT1 Antibody (NB110-41487R) (0)

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for DGAT1 Antibody (NB110-41487R) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin Labeled NB110-41487B
DyLight 488 Labeled NB110-41487G
DyLight 650 Labeled NB110-41487C
HRP Labeled NB110-41487H

Additional DGAT1 Products

DGAT1 NB110-41487R

Bioinformatics Tool for DGAT1 Antibody (NB110-41487R)

Discover related pathways, diseases and genes to DGAT1 Antibody (NB110-41487R). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Research Areas for DGAT1 Antibody (NB110-41487R)

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Blogs on DGAT1.

Can you DGAT? DGAT1 & DGAT2 Antibodies for Metabolic Pathway Research
Triacylglycerols (triglycerides) (TGs) are the major storage molecules of metabolic energy and FAs in most living organisms. Excessive accumulation of TGs, however, is associated with human diseases, such as obesity, diabetes mellitus, and steatohepat...  Read full blog post.

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Bioinformatics

Gene Symbol DGAT1