DAX1/NR0B1 Antibody (OTI5F5)

Western Blot: DAX1/NR0B1 Antibody (5F5) [NBP2-46212] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY NR0B1.

Immunohistochemistry: DAX1/NR0B1 Antibody (5F5) [NBP2-46212] - Analysis of Adenocarcinoma of Human colon tissue. (Heat-induced epitope retrieval by 1 mM EDTA in 10mM Tris, pH8.5, 120C for 3min)

• Reactivity: Hu
• Applications: WB, IHC
• Clone: OTI5F5
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1 mg/ml

DAX1/NR0B1 Antibody (OTI5F5) Summary

• Description: Novus Biologicals Mouse DAX1/NR0B1 Antibody (OTI5F5) (NBP2-46212) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Human recombinant protein fragment corresponding to amino acids 161-430 of human NR0B1 (NP_000466) produced in E.coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NR0B1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:150
  • Immunohistochemistry-Paraffin
  • Western Blot
• Theoretical MW: 51.5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 1.0% BSA and 50% Glycerol
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DAX1/NR0B1 Antibody (OTI5F5)

• AHC
• AHCDSS
• AHCH
• AHX
• DAX1
• DAX1DAX-1
• dosage-sensitive sex reversal
• DSS-AHC critical region on the X chromosome protein 1
• GTD
• HHG
• NR0B1
• NROB1
• nuclear hormone receptor
• Nuclear receptor DAX-1
• nuclear receptor subfamily 0 group B member 1
• nuclear receptor subfamily 0, group B, member 1

Background

NR0B1 encodes a protein that contains a DNA-binding domain. The encoded protein acts as a dominant-negative regulator of transcription which is mediated by the retinoic acid receptor. This protein also functions as an anti-testis gene by acting antagonistically to Sry. Mutations in this gene result in both X-linked congenital adrenal hypoplasia and hypogonadotropic hypogonadism.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NR0B1