CXCR4 Antibody - BSA Free

Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Brain Cerebellum Purkinje Neuron

Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Analysis of anti-CXCR4 antibody with human tonsil.

Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Analysis of anti-CXCR4 antibody with human colon, carcinoma.

Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Human Lymph Node, Non-Hodgkins Lymphoma formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.

Immunohistochemistry-Frozen: CXCR4 Antibody [NLS1380] - Rat brain tissue sections. Rat Nucleus Accumbens treated with MDPV. Images taken with fluorescent microscope. Primary antibody was diluted to 1:200. Secondary antibody used was donkey anti-Rabbit Alexa Fluor 594 at 1:500. IHC-Fr image submitted by a verified customer review.

Immunohistochemistry-Paraffin: CXCR4 Antibody [NLS1380] - Lung, small cell carcinoma

• Reactivity: Hu, Mu, Rt, Ch, Ha, Pm, Pm, Rb, Pm, Ma-Op, Pm-Or, Tr
• Applications: IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

CXCR4 Antibody - BSA Free Summary

• Description: Product can be stored undiluted at 4C for up to 1 month.
• Immunogen: Synthetic 20 amino acid peptide from 3rd cytoplasmic domain of human CXCR4.
• Epitope: 3rd cytoplasmic domain
• Localization: Integral membrane protein.
• Specificity: Human CXCR4. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Predicted Species: Orangutan (100%), Chimpanzee (100%), Opossum (100%), Turkey (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CXCR4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin 9-14 ug/mL
• Application Notes: CXCR4 antibody validated for IHC-Fr from a verified customer review..

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Gorilla (100%), Gibbon (100%), Baboon (100%), Marmoset (100%).

Packaging, Storage & Formulations

• Storage: Keep as concentrated solution. Aliquot and store at -20C or below. Avoid multiple freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.1% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CXCR4 Antibody - BSA Free

• CD184
• chemokine (C-X-C motif) receptor 4
• chemokine (C-X-C motif), receptor 4 (fusin)
• C-X-C chemokine receptor type 4
• CXCR4
• CXC-R4
• CXCR-4
• D2S201E
• D2S201ESDF-1 receptor
• FB22
• Fusin
• HM89
• HM89NPYRL
• HSY3RR
• LAP3
• LAP-3
• LCR1
• LESTR
• LESTRCD184 antigen
• Leukocyte-derived seven transmembrane domain receptor
• leukocyte-derived seven-transmembrane-domain receptor
• lipopolysaccharide-associated protein 3
• neuropeptide Y receptor Y3
• NPY3R
• NPY3RWHIM
• NPYR
• NPYRL
• NPYY3R
• pCXCR4
• seven transmembrane helix receptor
• seven-transmembrane-segment receptor, spleen
• Stromal cell-derived factor 1 receptor
• WHIMS

Background

CXCR4, a Chemokine Receptor involved in organ vascularization, neuronal cell migration, and patterning of the central nervous system during development. It binds stromal cell-derived factor 1 (SDF1, also called PBFS) and mediates migration of resting leukocytes and is unique in homing hematopoietic progenitors to bone marrow. These properties suggest that CXCR4 is involved in tumor cell migration and local tumor invasion. It is also an HIV-1 fusion co-factor that allows HIV-1 invasion in diverse human cell types. Although this receptor was initially called Neuropeptide Y3 Receptor, it does not respond to neuropeptide Y. CXCR4 has two isoforms that are produced by alternative splicing. CXCR4 has been reported to be expressed in cells of the immune and central nervous systems. It is highly expressed in brain and heart, and in white blood cells, vascular endothelial cells, and umbilical cord endothelial cells. In brain tumors, CXCR4 is expressed in regions of angiogenesis and degenerative, necrotic, and microcystic changes (Rempel et al., 2000). ESTs have been isolated from a wide variety of tissue libraries, particularly uterus, kidney, lung, tonsil, and brain.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR4 Antibody (NLS1380)(5)

Publications using NLS1380

• Rohlenova K, Goveia J, Garcia-Caballero M et al. Single-Cell RNA Sequencing Maps Endothelial Metabolic Plasticity in Pathological Angiogenesis Cell Metab. 2020-04-07 [PMID: 32268117]
• Goveia J, Rohlenova K, Taverna F et al. An Integrated Gene Expression Landscape Profiling Approach to Identify Lung Tumor Endothelial Cell Heterogeneity and Angiogenic Candidates Cancer Cell 2020-01-13 [PMID: 31935371]
• McLeod DS, Hasegawa T, Baba Tet al. From Blood Islands to Blood Vessels: Morphologic Observations and Expression of Key Molecules during Hyaloid Vascular System Development. Invest Ophthalmol Vis Sci 2012-12-03 [PMID: 23092923]
• Baba T, McLeod DS, Edwards MM et al. VEGF(165) b in the developing vasculatures of the fetal human eye. Dev Dyn. 2012-03-01 [PMID: 22275161]
• Bhutto, I et al. Localisation of SDF-1 its receptor CXCR4 in retina choroid of aged human eyes in eyes with age related macular degeneration. Bhutto, I. A., McLeod, D. S., Merges, C., Hasegawa, T., Lutty, G. A. (2006) Localisation of SDF-1 its receptor CXCR4 in retina choroid of aged human eyes in eyes with age related macular degeneration, 90, 906-910. Br. J. Ophthalmol; 90: 906-910. 2006-01-01 [PMID: 16597663]

Review for CXCR4 Antibody (NLS1380) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Rat.

reviewed by: Jeff Foss IHC-Fr Rat 08/20/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Brain Tissue Sections
• Species: Rat
• Lot: 5465

FAQs for CXCR4 Antibody (NLS1380). (Showing 1 - 1 of 1 FAQs).

1. Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.
   • CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

Bioinformatics

• Gene Symbol: CXCR4
• Entrez:
  • 7852(Human)
• Uniprot:
  • P61073()