CXCR4 Antibody

Western Blot: CXCR4 Antibody [NB100-56437] - Detection of 15 ug in HeLa cells with antibody concentration at 1 ug/ml.

Immunocytochemistry/Immunofluorescence: CXCR4 Antibody [NB100-56437] - Jurkat cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-CXCR4 (NB100-56437) at a 1:25 dilution for 1 hour at room temperature and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin was used as a co-stain at a 1:1000 dilution and detected with and anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

Immunohistochemistry-Paraffin: CXCR4 Antibody [NB100-56437] - CXCR4 was detected in immersion sections of human tonsil using rabbit anti-human antibody (Catalog # NB100-56437) at 1:300 dilution overnight at 4C. Tissue was stained using the VisuCyte anti-rabbit HRP polymer detection reagent with DAB chromogen (brown) and counterstained with hematoxylin (blue).

• Reactivity: Hu, Mu, Rt, Sh
• Applications: WB, ICC/IF, IHC, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

CXCR4 Antibody Summary

• Immunogen: Rabbit anti-CXCR4 polyclonal antibody was raised against a peptide corresponding to amino acids 328-338 of human CXCR4.
• Specificity: In HeLa, a 45 kDa band is observed.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CXCR4
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1-2 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
• Application Notes: Immunohistochemistry (cryosections): see Nimmagadda etl al (2010) for details. Immunohistochemistry (paraffin): see Zagzag (2005) and others for details.
• Control:

  HeLa Whole Cell Lysate
  CXCR4 Knockout HeLa Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for CXCR4 Antibody

• CD184
• chemokine (C-X-C motif) receptor 4
• chemokine (C-X-C motif), receptor 4 (fusin)
• C-X-C chemokine receptor type 4
• CXCR4
• CXC-R4
• CXCR-4
• D2S201E
• D2S201ESDF-1 receptor
• FB22
• Fusin
• HM89
• HM89NPYRL
• HSY3RR
• LAP3
• LAP-3
• LCR1
• LESTR
• LESTRCD184 antigen
• Leukocyte-derived seven transmembrane domain receptor
• leukocyte-derived seven-transmembrane-domain receptor
• lipopolysaccharide-associated protein 3
• neuropeptide Y receptor Y3
• NPY3R
• NPY3RWHIM
• NPYR
• NPYRL
• NPYY3R
• seven transmembrane helix receptor
• seven-transmembrane-segment receptor, spleen
• Stromal cell-derived factor 1 receptor
• WHIMS

Background

Human immunodeficiency virus (HIV) and related viruses require coreceptors, in addition to CD4, to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b and CCR8 in the chemokine receptor family, and four new human molecules GPR15, STRL33, GPR1 and V28 were recently identified as HIV coreceptors1. Among them, CXCR4 (fusin, LESTR or HUMSTR) is a principal coreceptor for T-cell tropic strains of HIV-1 fusion and entry of human white blood cells (2,3). CXCR4 is also required for the infection by dual-tropic strains of HIV-1 and mediates CD-4 independent infection by HIV-2 (4,5). The a-chemokine SDF-1 is the ligand for CXCR4 and prevents infection by T-tropic HIV-1 (6,7). CXCR4 associates with the surface CD4-gp120 complex before HIV enters target cells (8). CXCR4 messenger RNA levels correlated with HIV-1 permissiveness in diverse human cell types (2). Antibodies to CXCR4 block HIV-1 and HIV-2 fusion and infection of human target cells (2,5,10). The amino-terminal domain and the second extracellular loop of CXCR4 serve as HIV biding sites (10,11).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCR4 Antibody (NB100-56437)(15)

Publications using NB100-56437

• Pelagalli A, Nardelli A, Lucarelli E et al. Autocrine signals increase Ovine Mesenchymal Stem Cells migration through Aquaporin-1 and CXCR4 overexpression J. Cell. Physiol. 2018 Jan 18 [PMID: 29345324] (WB)
• Irie Y, Tsubota M, Ishikura H et al. Macrophage-derived HMGB1 as a Pain Mediator in the Early Stage of Acute Pancreatitis in Mice: Targeting RAGE and CXCL12/CXCR4 Axis J Neuroimmune Pharmacol 2017 Jul 28 [PMID: 28755135] (WB, Mouse)
• Gagner JP, Sarfraz Y, Ortenzi V et al. Multifaceted C-X-C Chemokine Receptor 4 Inhibition Interferes with Anti-Vascular Endothelial Growth Factor Therapy-Induced Glioma Dissemination. Am. J. Pathol. 2017 Jul 20 [PMID: 28734730] (WB, Mouse)
• Dieckow J, Brandt W, Hattermann K et al. CXCR4 and CXCR7 Mediate TFF3-Induced Cell Migration Independently From the ERK1/2 Signaling Pathway. Invest. Ophthalmol. Vis. Sci. 2016 Jan 01 [PMID: 26780310] (ICC/IF, Human)
• Ghosh MC, Makena PS, Gorantla V et al. CXCR4 regulates migration of lung alveolar epithelial cells through activation of Rac1 and matrix metalloproteinase-2. Am J Physiol Lung Cell Mol Physiol. 2012 May 1 [PMID: 22345572]
• De Silva RA, Peyre K, Pullambhatla M et al. Imaging CXCR4 expression in human cancer xenografts: evaluation of monocyclam 64Cu-AMD3465. J Nucl Med. 2011 Jun [PMID: 21622896]
• Nimmagadda S, Pullambhatla M, Stone K et al. Molecular imaging of CXCR4 receptor expression in human cancer xenografts with [64Cu]AMD3100 positron emission tomography. Cancer Res. 2010 May 15 [PMID: 20460522]
• Esencay M, Newcomb EW, Zagzag D. HGF upregulates CXCR4 expression in gliomas via NF-kappaB: implications for glioma cell migration. J Neurooncol. 2010 Aug [PMID: 20157762]
• Hogg K, Etherington SL, Young JM et al. Inhibitor of differentiation (Id) genes are expressed in the steroidogenic cells of the ovine ovary and are differentially regulated by members of the transforming growth factor-beta family. Endocrinology [PMID: 20032053]
  
  Details:
  Used the Azide Free form of this antibody.
• Pacheco-Rodriguez G, Kumaki F, Steagall WK et al. Chemokine-enhanced chemotaxis of lymphangioleiomyomatosis cells with mutations in the tumor suppressor TSC2 gene. J Immunol. 2009 Feb 1 [PMID: 19155472] (Human)
  
  Details:
  Antibodies cited [IHC(paraffin), human lung tissue, Fig. Supp 1]: 1. IMG-71201 (CCR1), Fig. 1D 2. IMG-72169 (CCR3) 3. IMG-71206 (CCR4) 4. IMG-5600-1 (CCR5) 5. IMG-5600-2 (CCR5).
• Zagzag D, Esencay M, Mendez O et al. Hypoxia- and vascular endothelial growth factor-induced stromal cell-derived factor-1alpha/CXCR4 expression in glioblastomas: one plausible explanation of Scherer's structures. Am J Pathol. 2008 Aug [PMID: 18599607] (WB, ICC/IF, Human)
  
  Details:
  IF (human glioma cell lines LN229, LN308, and U87), Fig. 3B; WB (human glioma cell lines LN229, LN308, and U87), Fig. 3A. Inhibitor of differentiation (Id) genes are expressed in the steriodogenic cells of the ovine ovary and are differentially regulated
• Ma YH, Mentlein R, Knerlich F et al. Expression of stem cell markers in human astrocytomas of different WHO grades. J Neurooncol. 2008 Jan [PMID: 17611714] (IHC-P, Human)
  
  Details:
  IHC (paraffin), human astrocytoma tissues, Fig. 4.
• Zagzag D, Lukyanov Y, Lan L et al. Hypoxia-inducible factor 1 and VEGF upregulate CXCR4 in glioblastoma: implications for angiogenesis and glioma cell invasion. Lab Invest. 2006 Dec [PMID: 17075581]
• Kulbe H, Hagemann T, Szlosarek PW et al. The inflammatory cytokine tumor necrosis factor-alpha regulates chemokine receptor expression on ovarian cancer cells. Cancer Res. 2005 Nov 15 [PMID: 16288025]
• Zagzag D, Krishnamachary B, Yee H et al. Stromal cell-derived factor-1alpha and CXCR4 expression in hemangioblastoma and clear cell-renal cell carcinoma: von Hippel-Lindau loss-of-function induces expression of a ligand and its receptor. Cancer Res. 2005 Jul 15 [PMID: 16024619]

FAQs for CXCR4 Antibody (NB100-56437). (Showing 1 - 1 of 1 FAQs).

1. Which is your best CXCR4 for immunohistochemistry in paraffin tissues? I would like to detect it in paraffin embedded tissues from human breast cancer samples.
   • CXCR4 antibody (NB100-74396) is our best selling product among all the CXCR4 antibodies and it has been validated for IHC-P in human cervical carcinoma tissue sections. NB100-74396 has been cited in at least 7 research publications. Additionally, here is a list of all of our CXCR4 antibodies that has been verified in IHC-P in human samples.

Bioinformatics

• Gene Symbol: CXCR4
• Entrez:
  • 7852(Human)
  • 12767(Mouse)
  • 60628(Rat)
• Uniprot:
  • P61073()