Mouse CXCL12/SDF-1 ELISA Kit (Colorimetric)

ELISA: Mouse CXCL12/SDF-1 ELISA Kit (Colorimetric) [NBP3-18746] - Samples were spiked with high concentrations of Mouse CXCL12/SDF-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse CXCL12/SDF-1 ELISA Kit (Colorimetric) [NBP3-18746] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 78.13-5000 pg/mL
• Sensitivity: 46.88 pg/mL

Mouse CXCL12/SDF-1 ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse CXCL12/SDF-1 in samples. No significant cross-reactivity or interference between Mouse CXCL12/SDF-1 and analogues was observed.
• Standard Curve Range: 78.13-5000 pg/mL
• Sensitivity: 46.88 pg/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: %CV < 5.26
• Intra-Assay: %CV < 5.78
• Spike Recovery: 88-113%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CXCL12

Applications/Dilutions

• Dilutions:
  • ELISA
  • Sandwich ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse CXCL12/SDF-1 ELISA Kit (Colorimetric)

• chemokine (C-X-C motif) ligand 12
• C-X-C motif chemokine 12
• CXCL12
• hIRH
• hSDF-1
• Intercrine reduced in hepatomas
• IRH
• PBSF
• PBSFSDF-1a
• Pre-B cell growth-stimulating factor
• SCYB12
• SDF1
• SDF-1
• SDF1A
• SDF1B
• SDF1SDF-1b
• stromal cell-derived factor 1
• TLSF
• TLSF-a
• TLSF-b
• TPAR1

Background

The C-X-C or alpha chemokine family is characterized by a pair of cysteine residues separated by a single amino acid and primarily functions as chemoattractants for neutrophils. The C-X-C family includes IL-8, NAP-2, MSGA and stromal cell-derived factor-1, or SDF-1. SDF-1 was originally described as a pre-B cell stimulatory factor, but has now been shown to function as a potent chemoattractant for T cells and monocytes, but not neutrophils. Receptors for the C-X-C family are G protein-coupled, seven-pass, transmembrane domain proteins which include IL-8RA, IL-8RB and fusin (also designated LESTR or CXCR-4). Fusin is highly homologous to the IL-8 receptors, sharing 37% sequence identity at the amino acid level. The IL-8 receptors bind to IL-8, NAP-2 and MSGA, while fusin binds to its cognate ligand, SDF-1. Fusin has been identified as the major co-receptor for T-tropic HIV-1, and SDF-1 has been shown to inhibit HIV-1 infection. Six human SDF-1 isoforms exist due to alternative splicing of CXCL12, the gene encoding SDF-1. Three isoforms are known for mouse and rat.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CXCL12