CXCL13/BLC/BCA-1 Antibody

Images

 
Recombinant Mouse CXCL13/BLC/BCA‑1 (Catalog # 470-BC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more
CXCL13/BLC/BCA‑1 was detected in perfusion fixed frozen sections of mouse intestine (Peyer's patch) using Goat Anti-Mouse CXCL13/BLC/BCA‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF470) at 15 µg/mL ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, Neut
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price

CXCL13/BLC/BCA-1 Antibody Summary

Immunogen
E. coli-derived recombinant mouse CXCL13/BLC/BCA‑1
Ile22-Ala109
Accession # Q3U1E8
Specificity
Detects mouse CXCL13/BLC/BCA‑1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CXCL13 and rhGCP-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CXCL13
Purity
Immunogen affinity purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 20-50 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF470 in the following applications:

Publications
Read Publications using
AF470 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL13/BLC/BCA-1 Antibody

  • ANGIE
  • ANGIE2
  • B cell-attracting chemokine 1
  • B lymphocyte chemoattractant
  • BCA1
  • BCA-1
  • BCA1B-cell chemoattractant
  • BCA-1CXC chemokine BLC
  • B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1)
  • BLC
  • BLCSmall-inducible cytokine B13
  • BLR1L
  • B-lymphocyte chemoattractant
  • chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant)
  • chemokine (C-X-C motif) ligand 13
  • C-X-C motif chemokine 13
  • CXCL13
  • SCYB13B-cell-attracting chemokine 1
  • small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)

Background

CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. Mouse BCA-1 cDNA encodes a precursor protein of 109 amino acid residues with a putative leader sequence of 21 residues. Mature mouse BCA-1 shares 64% amino acid sequence similarity with the human protein and 23‑34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCL13/BLC/BCA-1 Antibody (AF470)(19)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 9 applications: Flow, ICC, IHC, IHC - Frozen, IHC Fresh, IHC Frozen, IHC Paraffin-embedded, Neut, Western Blot.


Filter By Application
Flow
(1)
ICC
(1)
IHC
(5)
IHC - Frozen
(1)
IHC Fresh
(1)
IHC Frozen
(5)
IHC Paraffin-embedded
(3)
Neut
(1)
Western Blot
(1)
All Applications
Filter By Species
Mouse
(19)
All Species
Showing Publications 1 - 10 of 19. Show All 19 Publications.
Publications using AF470 Applications Species
T Eddens, W Elsegeiny, ML Garcia-Her, P Castillo, G Trevejo-Nu, K Serody, BT Campfield, SA Khader, K Chen, J Rangel-Mor, JK Kolls Pneumocystis-Driven Inducible Bronchus-Associated Lymphoid Tissue Formation Requires Th2 and Th17 Immunity Cell Rep, 2017;18(13):3078-3090. 2017 [PMID: 28355561] (IHC,, Mouse) IHC Mouse
Brain inflammation is accompanied by peripheral inflammation in Cstb (-/-) mice, a model for progressive myoclonus epilepsy J Neuroinflammation, 2016;13(1):298. 2016 [PMID: 27894304] (IHC - Frozen,, Mouse) IHC - Frozen Mouse
Hif1a Deletion Reveals Pro-Neoplastic Function of B Cells in Pancreatic Neoplasia Cancer Discov, 2016;6(3):256-69. 2016 [PMID: 26715642] (Western Blot,, Mouse) Western Blot Mouse
Buettner M, Dittrich-Breiholz O, Falk C, Lochner M, Smoczek A, Menzel F, Bornemann M, Bode U Stromal cells as trend-setters for cells migrating into the lymph node. Mucosal Immunol, 2015;8(3):640-9. 2015 [PMID: 25354321] (IHC Frozen,, Mouse) IHC Frozen Mouse
Fleige H, Ravens S, Moschovakis G, Bolter J, Willenzon S, Sutter G, Haussler S, Kalinke U, Prinz I, Forster R IL-17-induced CXCL12 recruits B cells and induces follicle formation in BALT in the absence of differentiated FDCs. J Exp Med, 2014;211(4):643-51. 2014 [PMID: 24663215] (IHC,, Mouse) IHC Mouse
Yamamoto, K, Nishiumi, S, Yang, L, Klimatcheva, E, Pandina, T, Takahashi, S, Matsui, H, Nakamura, M, Zauderer, M, Yoshida, M, Azuma, T Anti-CXCL13 antibody can inhibit the formation of gastric lymphoid follicles induced by Helicobacter infection. Mucosal Immunol, 2014;7(5):1244-54. 2014 [PMID: 24646940] (Neut,, Mouse) Neut Mouse
Benezech C, Nayar S, Finney B, Withers D, Lowe K, Desanti G, Marriott C, Watson S, Caamano J, Buckley C, Barone F CLEC-2 is required for development and maintenance of lymph nodes. Blood, 2014;123(20):3200-7. 2014 [PMID: 24532804] (IHC Frozen,, Mouse) IHC Frozen Mouse
Monson N, Ortega S, Ireland S, Meeuwissen A, Chen D, Plautz E, Shubel E, Kong X, Li M, Freriks L, Stowe A Repetitive hypoxic preconditioning induces an immunosuppressed B cell phenotype during endogenous protection from stroke. J Neuroinflammation, 2014;11(0):22. 2014 [PMID: 24485041] (IHC,, Mouse) IHC Mouse
Mionnet C, Mondor I, Jorquera A, Loosveld M, Maurizio J, Arcangeli M, Ruddle N, Nowak J, Aurrand-Lions M, Luche H, Bajenoff M Identification of a new stromal cell type involved in the regulation of inflamed B cell follicles. PLoS Biol, 2013;11(10):e1001672. 2013 [PMID: 24130458] (ICC,, Mouse) ICC Mouse
Kramer J, Klimatcheva E, Rothstein T CXCL13 is elevated in Sjogren&#039;s syndrome in mice and humans and is implicated in disease pathogenesis. J Leukoc Biol, 2013;94(5):1079-89. 2013 [PMID: 23904442] (IHC,, Mouse) IHC Mouse
Show All 19 Publications.

Reviews for CXCL13/BLC/BCA-1 Antibody (AF470) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.
We have 2 reviews tested in 2 application: IHC-Fr, IF.

Reviews using AF470:
Filter by Applications
IHC-Fr
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-Fr Mouse 01/08/2015
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 20643338
SpeciesMouse
 
reviewed by:
Anonymous
IF Mouse 01/08/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 21593383
SpeciesMouse

Blocking

Blocking DetailsSee PMID 21593383

Details

Detection NotesSee PMID 21593383

Comments

CommentsPublished: See PMID 21593383Independent review from antibodyresource.comStrong specific labeling of FDCs with only minimal background in murine spleen.

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

 
Anonymous
01/08/2015
Application: IHC-Fr
Species: Mouse

 
Anonymous
01/08/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol CXCL13
Entrez
Uniprot