CXCL13/BLC/BCA-1 Antibody

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CXCL13/BLC/BCA‑1 was detected in perfusion fixed frozen sections of mouse intestine (Peyer's patch) using Goat Anti-Mouse CXCL13/BLC/BCA‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF470) at 15 µg/mL ...read more
Recombinant Mouse CXCL13/BLC/BCA‑1 (Catalog # 470-BC) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to ...read more

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IHC, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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CXCL13/BLC/BCA-1 Antibody Summary

Immunogen
E. coli-derived recombinant mouse CXCL13/BLC/BCA‑1
Ile22-Ala109
Accession # Q3U1E8
Specificity
Detects mouse CXCL13/BLC/BCA‑1 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) CXCL13 and rhGCP-2 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CXCL13
Purity
Immunogen affinity purified
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 5-15 ug/mL
  • Neutralization 20-50 ug/mL
  • Western Blot 0.1 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF470 in the following applications:

Publications
Read Publications using
AF470 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL13/BLC/BCA-1 Antibody

  • ANGIE
  • ANGIE2
  • B cell-attracting chemokine 1
  • B lymphocyte chemoattractant
  • BCA1
  • BCA-1
  • BCA1B-cell chemoattractant
  • BCA-1CXC chemokine BLC
  • B-cell-homing chemokine (ligand for Burkitt's lymphoma receptor-1)
  • BLC
  • BLCSmall-inducible cytokine B13
  • BLR1L
  • B-lymphocyte chemoattractant
  • chemokine (C-X-C motif) ligand 13 (B-cell chemoattractant)
  • chemokine (C-X-C motif) ligand 13
  • C-X-C motif chemokine 13
  • CXCL13
  • SCYB13
  • SCYB13B-cell-attracting chemokine 1
  • small inducible cytokine B subfamily (Cys-X-Cys motif), member 13 (B-cellchemoattractant)

Background

CXCL13, also known as B-lymphocyte chemoattractant (BLC), is a CXC chemokine that is constitutively expressed in secondary lymphoid organs. Mouse BCA-1 cDNA encodes a precursor protein of 109 amino acid residues with a putative leader sequence of 21 residues. Mature mouse BCA-1 shares 64% amino acid sequence similarity with the human protein and 23‑34% amino acid sequence identity with other known CXC chemokines. Recombinant or chemically synthesized BCA-1 is a potent chemoattractant for B lymphocytes but not T lymphocytes, monocytes or neutrophils. BLR1, a G protein-coupled receptor originally isolated from Burkitt’s lymphoma cells, has now been shown to be the specific receptor for BCA-1. Among cells of the hematopoietic lineages, the expression of BLR1, now designated CXCR5, is restricted to B lymphocytes and a subpopulation of T helper memory cells. Mice lacking BLR1 have been shown to lack inguinal lymph nodes. These mice were also found to have impaired development of Peyer’s patches and defective formation of primary follicles and germinal centers in the spleen as a result of the inability of B lymphocytes to migrate into B cell areas.

  1. Gunn, M.D. et al. (1998) Nature, 391:799.
  2. Legler, D.F. et al. (1998) J. Exp. Med. 187:655.
  3. Forster, R. et al. (1996) Cell 87:1037.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCL13/BLC/BCA-1 Antibody (AF470)(36)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 11 applications: Flow Cytometry, ICC, ICC/IF, IHC, IHC-F, IHC-Fr, IHC-P, In Vivo, Migration Assay, Neutralization, Western Blot.


Filter By Application
Flow Cytometry
(2)
ICC
(1)
ICC/IF
(1)
IHC
(11)
IHC-F
(1)
IHC-Fr
(10)
IHC-P
(5)
In Vivo
(1)
Migration Assay
(1)
Neutralization
(1)
Western Blot
(2)
All Applications
Filter By Species
Mouse
(36)
All Species
Showing Publications 1 - 10 of 36. Show All 36 Publications.
Publications using AF470 Applications Species
MC Trolese, A Mariani, M Terao, M de Paola, P Fabbrizio, F Sironi, M Kurosaki, S Bonanno, S Marcuzzo, P Bernasconi, F Trojsi, E Aronica, C Bendotti, G Nardo CXCL13/CXCR5 signalling is pivotal to preserve motor neurons in amyotrophic lateral sclerosis EBioMedicine, 2020;62(0):103097. 2020 [PMID: 33161233] (IHC, Mouse) IHC Mouse
QF Zhang, J Li, K Jiang, R Wang, JL Ge, H Yang, SJ Liu, LT Jia, L Wang, BL Chen CDK4/6 inhibition promotes immune infiltration in ovarian cancer and synergizes with PD-1 blockade in a B cell-dependent manner Theranostics, 2020;10(23):10619-10633. 2020 [PMID: 32929370] (Migration Assay, Mouse) Migration Assay Mouse
K Streich, M Smoczek, J Hegermann, O Dittrich-B, M Bornemann, A Siebert, A Bleich, M Buettner Dietary lipids accumulate in macrophages and stromal cells and change the microarchitecture of mesenteric lymph nodes J Adv Res, 2020;24(0):291-300. 2020 [PMID: 32405435] (IHC, Mouse) IHC Mouse
N Muthalagu, T Monteverde, X Raffo-Irao, R Wiesheu, D Whyte, A Hedley, S Laing, B Kruspig, R Upstill-Go, R Shaw, S Neidler, C Rink, SA Karim, K Gyuraszova, C Nixon, W Clark, AV Biankin, LM Carlin, SB Coffelt, OJ Sansom, JP Morton, DJ Murphy Repression of the Type I Interferon pathway underlies MYC &amp; KRAS-dependent evasion of NK &amp; B cells in Pancreatic Ductal Adenocarcinoma Cancer Discov, 2020;0(0):. 2020 [PMID: 32200350] (In Vivo, Mouse) In Vivo Mouse
X Jia, F Gábris, Ó Jacobsen, G Bedics, B Botz, Z Helyes, Z Kellermaye, D Vojkovics, G Berta, N Nagy, Z Jakus, P Balogh Foliate Lymphoid Aggregates as Novel Forms of Serous Lymphocyte Entry Sites of Peritoneal B Cells and High-Grade B Cell Lymphomas J. Immunol., 2019;0(0):. 2019 [PMID: 31767783] (Flow Cytometry, Mouse) Flow Cytometry Mouse
LK Dubey, B Ludewig, SA Luther, NL Harris IL-4R?-Expressing B Cells Are Required for CXCL13 Production by Fibroblastic Reticular Cells Cell Rep, 2019;27(8):2442-2458.e5. 2019 [PMID: 31116987] (IHC-F, Mouse) IHC-F Mouse
AC Billi, M Gharaee-Ke, J Fullmer, LC Tsoi, BD Hill, D Gruszka, J Ludwig, X Xing, S Estadt, SJ Wolf, SM Rizvi, CC Berthier, JB Hodgin, MA Beamer, MK Sarkar, Y Liang, R Uppala, S Shao, C Zeng, PW Harms, ME Verhaegen, JJ Voorhees, F Wen, NL Ward, AA Dlugosz, JM Kahlenberg, JE Gudjonsson The female-biased factor VGLL3 drives cutaneous and systemic autoimmunity JCI Insight, 2019;4(8):. 2019 [PMID: 30996136] (ICC/IF, Mouse) ICC/IF Mouse
HW Cheng, L Onder, M Novkovic, C Soneson, M Lütge, N Pikor, E Scandella, MD Robinson, JI Miyazaki, A Tersteegen, U Sorg, K Pfeffer, T Rülicke, T Hehlgans, B Ludewig Origin and differentiation trajectories of fibroblastic reticular cells in the splenic white pulp Nat Commun, 2019;10(1):1739. 2019 [PMID: 30988302] (IHC, Mouse) IHC Mouse
M Kim, SH Lee, Y Kim, Y Kwon, Y Park, HK Lee, HS Jung, D Jeoung Human Adipose Tissue-Derived Mesenchymal Stem Cells Attenuate Atopic Dermatitis by Regulating the Expression of MIP-2, miR-122a-SOCS1 Axis, and Th1/Th2 Responses Front Pharmacol, 2018;9(0):1175. 2018 [PMID: 30459600] (IHC, Mouse) IHC Mouse
M Gallotta, H Assi, É Degagné, SK Kannan, RL Coffman, C Guiducci Inhaled TLR9 Agonist Renders Lung Tumors Permissive to PD-1 Blockade by Promoting Optimal CD4+ and CD8+ T cell Interplay Cancer Res., 2018;0(0):. 2018 [PMID: 29945961] (IHC-P, Mouse) IHC-P Mouse
Show All 36 Publications.

Reviews for CXCL13/BLC/BCA-1 Antibody (AF470) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Mouse.

Reviews using AF470:
Filter by Applications
IHC-Fr
(1)
IF
(1)
All Applications
Filter by Species
Mouse
(2)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Verified Customer
IHC-Fr Mouse 01/08/2015
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Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedSee PMID 20643338
SpeciesMouse
  4
reviewed by:
Verified Customer
IF Mouse 01/08/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 21593383
SpeciesMouse

Blocking

Blocking DetailsSee PMID 21593383

Details

Detection NotesSee PMID 21593383

Comments

CommentsPublished: See PMID 21593383Independent review from antibodyresource.comStrong specific labeling of FDCs with only minimal background in murine spleen.

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Secondary Antibodies

 

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Biotin BAF470

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Verified Customer
01/08/2015
Application: IHC-Fr
Species: Mouse

 
Verified Customer
01/08/2015
Application: IF
Species: Mouse

Bioinformatics

Gene Symbol CXCL13
Uniprot