DPPIV/CD26 Antibody [Unconjugated]

Images

 
Western blot shows lysate of LoVo human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) ...read more
Western blot shows lysate of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) ...read more
Simple Western lane view shows lysates of LoVo human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for DPPIV/CD26 at approximately 168 kDa (as indicated) using 2 µg/mL ...read more
DPPIV/CD26 was detected in immersion fixed paraffin-embedded sections of human psoriatic skin using Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) at 1 µg/mL overnight at 4 ...read more
DPPIV/CD26 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) at 1.7 µg/mL for 3 hours at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

DPPIV/CD26 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human DPPIV/CD26 (Catalog # 1180-SE)
Asp34-Pro766
Accession # Q53TN1
Specificity
Detects human DPPIV/CD26 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DPP4
Purity Statement
Antigen Affinity-purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry 1-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Simple Western 2 ug/mL
  • Western Blot 0.2 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF1180 in the following applications:

Publications
Read Publications using
AF1180 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DPPIV/CD26 Antibody [Unconjugated]

  • ADABP
  • ADCP-2
  • ADCP2DPP IV
  • Adenosine deaminase complexing protein 2TP103
  • CD26 antigen
  • CD26
  • CD26T-cell activation antigen CD26
  • dipeptidyl peptidase 4
  • Dipeptidyl peptidase IV
  • dipeptidylpeptidase 4
  • dipeptidyl-peptidase 4
  • dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
  • DPP4
  • DPPIV
  • EC 3.4.14.5

Background

DPPIV/CD26 (EC 3.4.14.5) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides. It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain. The extracellular domain contains glycosylation sites, a cysteine-rich region and the catalytic active site (Ser, Asp and His charge relay system). The amino acid sequence of the mouse DPPIV/CD26 extracellular domain is 84% and 91% identical to the human and rat counterparts, respectively. In the native state, DPPIV/CD26 is present as a noncovalently linked homodimer on the cell surface of a variety of cell types. The soluble form is also detectable in human serum and other body fluids, the levels of which may have clinical significance in patients with cancer, liver and kidney diseases, and depression.

DPPIV/CD26 plays an important role in many biological and pathological processes. It functions as T cell-activating molecule (THAM). It serves as a cofactor for entry of HIV in CD4+ cells. It binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans. It cleaves chemokines such as stromal-cell-derived factor 1 alpha and macrophage-derived chemokine. It degrades peptide hormones such as glucagon. It truncates procalcitonin, a marker for systemic bacterial infections with elevated levels detected in patients with thermal injury, sepsis and severe infection, and in children with bacterial meningitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DPPIV/CD26 Antibody (AF1180)(31)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Bat, Camelid, Ferret, Primate.

We have publications tested in 8 applications: Blocking, Flow Cytometry, ICC, IHC, IHC-P, Immunoprecipitation, Neutralization, Western Blot.


Filter By Application
Blocking
(1)
Flow Cytometry
(3)
ICC
(3)
IHC
(7)
IHC-P
(2)
Immunoprecipitation
(1)
Neutralization
(2)
Western Blot
(11)
All Applications
Filter By Species
Human
(24)
Mouse
(1)
Rat
(1)
Bat
(1)
Camelid
(1)
Ferret
(1)
Primate
(1)
All Species
Showing Publications 1 - 10 of 31. Show All 31 Publications.
Publications using AF1180 Applications Species
C Nadolny, X Zhang, Q Chen, SF Hashmi, W Ali, C Hemme, N Ahsan, Y Chen, R Deng Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma American journal of cancer research, 2021;11(10):4746-4767. 2021 [PMID: 34765291] (Western Blot, Human) Western Blot Human
Y Ogawa, Y Akimoto, M Ikemoto, Y Goto, A Ishikawa, S Ohta, Y Takase, H Kawakami, M Tsujimoto, R Yanoshita Stability of human salivary extracellular vesicles containing dipeptidyl peptidase IV under simulated gastrointestinal tract conditions Biochemistry and Biophysics Reports, 2021;27(0):101034. 2021 [PMID: 34141904] (Western Blot, Human) Western Blot Human
S Kamada, T Namekawa, K Ikeda, T Suzuki, M Kagawa, H Takeshita, A Yano, K Okamoto, T Ichikawa, K Horie-Inou, S Kawakami, S Inoue Functional inhibition of cancer stemness-related protein DPP4 rescues tyrosine kinase inhibitor resistance in renal cell carcinoma Oncogene, 2021;0(0):. 2021 [PMID: 33972682] (Western Blot, Human) Western Blot Human
S Takagi, M Hirokawa, K Nagashima, M Higuchi, K Kadota, R Ishikawa, M Sato, A Miyauchi, Y Miyake, R Haba Diagnostic significance of apical membranous and cytoplasmic dot-like CD26 expression in encapsulated follicular variant of papillary thyroid carcinoma: a useful marker for capsular invasion Endocr. J., 2020;0(0):. 2020 [PMID: 32879160] (IHC, Human) IHC Human
TJ Kolanowski, N Rozwadowsk, A Zimna, M Nowaczyk, M Siatkowski, W ?ab?d?, E Wiland, J Gapi?ski, S Jurga, M Kurpisz Chromatin and transcriptome changes in human myoblasts show spatio-temporal correlations and demonstrate DPP4 inhibition in differentiated myotubes Sci Rep, 2020;10(1):14336. 2020 [PMID: 32868771] (Western Blot, Human) Western Blot Human
TJ Kolanowski, N Rozwadowsk, A Zimna, M Nowaczyk, M Siatkowski, W ?ab?d?, E Wiland, J Gapi?ski, S Jurga, M Kurpisz Chromatin and transcriptome changes in human myoblasts show spatio-temporal correlations and demonstrate DPP4 inhibition in differentiated myotubes Sci Rep, 2020;10(1):14336. 2020 [PMID: 32868771] (Western Blot, Human) Western Blot Human
W Peng, D Rao, M Zhang, Y Shi, J Wu, G Nie, Q Xia Teneligliptin prevents doxorubicin-induced inflammation and apoptosis in H9c2 cells Arch. Biochem. Biophys., 2019;0(0):108238. 2019 [PMID: 31881187] (Western Blot, Rat) Western Blot Rat
Menachery V, Dinnon K, Yount B, McAnarney E, Gralinski L, Hale A, Graham R, Scobey T, Anthony S, Wang L, Graham B, Randell S, Lipkin W, Baric R Trypsin Treatment Unlocks Barrier for Zoonotic Bat Coronavirus Infection. J Virol, 0;94(5):. 0 [PMID: 31801868] (Human) Human
YN Chen, HC Hsu, SW Wang, HC Lien, HT Lu, SK Peng Entry of Scotophilus Bat Coronavirus-512 and Severe Acute Respiratory Syndrome Coronavirus in Human and Multiple Animal Cells Pathogens, 2019;8(4):. 2019 [PMID: 31766704] (ICC, Human) ICC Human
N Schmid, F Flenkentha, JB Stöckl, KG Dietrich, FM Köhn, JU Schwarzer, L Kunz, M Luckner, G Wanner, GJ Arnold, T Fröhlich, A Mayerhofer Insights into replicative senescence of human testicular peritubular cells Sci Rep, 2019;9(1):15052. 2019 [PMID: 31636313] (IHC, Human) IHC Human
Show All 31 Publications.

Reviews for DPPIV/CD26 Antibody (AF1180) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using AF1180:
Filter by Applications
IHC-Fr
(1)
IHC-P
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunohistochemistry-Frozen DPPIV/CD26 AF1180
Enlarge
4
reviewed by:
Verified Customer
IHC-Fr Human 02/22/2019
View

Summary

ApplicationImmunohistochemistry-Frozen
Sample TestedFrozen human skin sections
SpeciesHuman
LotJOQ0218011
  4
reviewed by:
Anonymous -
IHC-P Human 01/05/2015
View

Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 23638030
SpeciesHuman

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

Verified Customer
02/22/2019
Application: IHC-Fr
Species: Human

 
Anonymous -
01/05/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol DPP4
Uniprot