DPPIV/CD26 Antibody [Unconjugated]

Western blot shows lysate of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows lysate of LoVo human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DPPIV/CD26 at approximately 110 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

DPPIV/CD26 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm and plasma membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

DPPIV/CD26 was detected in immersion fixed paraffin-embedded sections of human psoriatic skin using Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) at 1 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to keratinocytes. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Simple Western lane view shows lysates of LoVo human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for DPPIV/CD26 at approximately 168 kDa (as indicated) using 2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu
• Applications: WB, Simple Western, IHC, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

DPPIV/CD26 Antibody [Unconjugated] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human DPPIV/CD26 (Catalog # 1180-SE)
  Asp34-Pro766
  Accession # Q53TN1
• Specificity: Detects human DPPIV/CD26 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DPP4
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry 1-15 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Simple Western 2 ug/mL
  • Western Blot 0.2 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DPPIV/CD26 Antibody [Unconjugated]

• ADABP
• ADCP-2
• ADCP2DPP IV
• Adenosine deaminase complexing protein 2TP103
• CD26 antigen
• CD26
• CD26T-cell activation antigen CD26
• dipeptidyl peptidase 4
• Dipeptidyl peptidase IV
• dipeptidylpeptidase 4
• dipeptidyl-peptidase 4
• dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
• DPP4
• DPPIV
• EC 3.4.14.5

Background

DPPIV/CD26 (EC 3.4.14.5) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides. It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain. The extracellular domain contains glycosylation sites, a cysteine-rich region and the catalytic active site (Ser, Asp and His charge relay system). The amino acid sequence of the mouse DPPIV/CD26 extracellular domain is 84% and 91% identical to the human and rat counterparts, respectively. In the native state, DPPIV/CD26 is present as a noncovalently linked homodimer on the cell surface of a variety of cell types. The soluble form is also detectable in human serum and other body fluids, the levels of which may have clinical significance in patients with cancer, liver and kidney diseases, and depression.

DPPIV/CD26 plays an important role in many biological and pathological processes. It functions as T cell-activating molecule (THAM). It serves as a cofactor for entry of HIV in CD4⁺ cells. It binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans. It cleaves chemokines such as stromal-cell-derived factor 1 alpha and macrophage-derived chemokine. It degrades peptide hormones such as glucagon. It truncates procalcitonin, a marker for systemic bacterial infections with elevated levels detected in patients with thermal injury, sepsis and severe infection, and in children with bacterial meningitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DPPIV/CD26 Antibody (AF1180)(33)

Publications using AF1180

• KMC Klee, MW Hess, M Lohmüller, S Herzog, K Pfaller, T Müller, GF Vogel, LA Huber A CRISPR screen in intestinal epithelial cells identifies novel factors for polarity and apical transport Elife, 2023-01-20;12(0):. 2023-01-20 [PMID: 36661306] (Flow Cytometry, ICC, Human)
• J Beumer, J Bauzá-Mart, TS Veth, V Geurts, C Boot, H Gilliam-Vi, SS Poulsen, FK Knop, W Wu, H Clevers Mapping prohormone processing by proteases in human enteroendocrine cells using genetically engineered organoid models Proceedings of the National Academy of Sciences of the United States of America, 2022-11-07;119(46):e2212057119. 2022-11-07 [PMID: 36343264] (IHC, Human)
• T Turunen, M Magris, M Malinen, J Kekäläinen Seminal-Plasma-Mediated Effects on Sperm Performance in Humans Cells, 2022-07-08;11(14):. 2022-07-08 [PMID: 35883590] (Western Blot, Human)
• C Nadolny, X Zhang, Q Chen, SF Hashmi, W Ali, C Hemme, N Ahsan, Y Chen, R Deng Dysregulation and activities of ubiquitin specific peptidase 2b in the pathogenesis of hepatocellular carcinoma American journal of cancer research, 2021-10-15;11(10):4746-4767. 2021-10-15 [PMID: 34765291] (Western Blot, Human)
• Y Ogawa, Y Akimoto, M Ikemoto, Y Goto, A Ishikawa, S Ohta, Y Takase, H Kawakami, M Tsujimoto, R Yanoshita Stability of human salivary extracellular vesicles containing dipeptidyl peptidase IV under simulated gastrointestinal tract conditions Biochemistry and Biophysics Reports, 2021-06-03;27(0):101034. 2021-06-03 [PMID: 34141904] (Western Blot, Human)
• S Kamada, T Namekawa, K Ikeda, T Suzuki, M Kagawa, H Takeshita, A Yano, K Okamoto, T Ichikawa, K Horie-Inou, S Kawakami, S Inoue Functional inhibition of cancer stemness-related protein DPP4 rescues tyrosine kinase inhibitor resistance in renal cell carcinoma Oncogene, 2021-05-10;0(0):. 2021-05-10 [PMID: 33972682] (IHC, Western Blot, Human)
• S Takagi, M Hirokawa, K Nagashima, M Higuchi, K Kadota, R Ishikawa, M Sato, A Miyauchi, Y Miyake, R Haba Diagnostic significance of apical membranous and cytoplasmic dot-like CD26 expression in encapsulated follicular variant of papillary thyroid carcinoma: a useful marker for capsular invasion Endocr. J., 2020-09-02;0(0):. 2020-09-02 [PMID: 32879160] (IHC, Human)
• TJ Kolanowski, N Rozwadowsk, A Zimna, M Nowaczyk, M Siatkowski, W ?ab?d?, E Wiland, J Gapi?ski, S Jurga, M Kurpisz Chromatin and transcriptome changes in human myoblasts show spatio-temporal correlations and demonstrate DPP4 inhibition in differentiated myotubes Sci Rep, 2020-08-31;10(1):14336. 2020-08-31 [PMID: 32868771] (Western Blot, Human)
• W Peng, D Rao, M Zhang, Y Shi, J Wu, G Nie, Q Xia Teneligliptin prevents doxorubicin-induced inflammation and apoptosis in H9c2 cells Arch. Biochem. Biophys., 2019-12-24;0(0):108238. 2019-12-24 [PMID: 31881187] (Western Blot, Rat)
• Menachery V, Dinnon K, Yount B, McAnarney E, Gralinski L, Hale A, Graham R, Scobey T, Anthony S, Wang L, Graham B, Randell S, Lipkin W, Baric R Trypsin Treatment Unlocks Barrier for Zoonotic Bat Coronavirus Infection. J Virol, 2020-02-14;94(5):. 2020-02-14 [PMID: 31801868] (Human)
• YN Chen, HC Hsu, SW Wang, HC Lien, HT Lu, SK Peng Entry of Scotophilus Bat Coronavirus-512 and Severe Acute Respiratory Syndrome Coronavirus in Human and Multiple Animal Cells Pathogens, 2019-11-22;8(4):. 2019-11-22 [PMID: 31766704] (ICC, Human)
• N Schmid, F Flenkentha, JB Stöckl, KG Dietrich, FM Köhn, JU Schwarzer, L Kunz, M Luckner, G Wanner, GJ Arnold, T Fröhlich, A Mayerhofer Insights into replicative senescence of human testicular peritubular cells Sci Rep, 2019-10-21;9(1):15052. 2019-10-21 [PMID: 31636313] (IHC, Human)
• R Hatano, T Yamada, H Madokoro, H Otsuka, E Komiya, T Itoh, Y Narita, S Iwata, H Yamazaki, S Matsuoka, NH Dang, K Ohnuma, C Morimoto Development of novel monoclonal antibodies with specific binding affinity for denatured human CD26 in formalin-fixed paraffin-embedded and decalcified specimens PLoS ONE, 2019-06-13;14(6):e0218330. 2019-06-13 [PMID: 31194830] (Flow Cytometry, Human)
• AS Cockrell, JC Johnson, IN Moore, DX Liu, KW Bock, MG Douglas, RL Graham, J Solomon, L Torzewski, C Bartos, R Hart, RS Baric, RF Johnson A spike-modified Middle East respiratory syndrome coronavirus (MERS-CoV) infectious clone elicits mild respiratory disease in infected rhesus macaques Sci Rep, 2018-07-16;8(1):10727. 2018-07-16 [PMID: 30013082] (IHC, Primate)
• AK Haverkamp, A Lehmbecker, I Spitzbarth, W Widagdo, BL Haagmans, J Segalés, J Vergara-Al, A Bensaid, JMA van den Br, ADME Osterhaus, W Baumgärtne Experimental infection of dromedaries with Middle East respiratory syndrome-Coronavirus is accompanied by massive ciliary loss and depletion of the cell surface receptor dipeptidyl peptidase 4 Sci Rep, 2018-06-27;8(1):9778. 2018-06-27 [PMID: 29950581] (ICC, Camelid)
• H Chu, CM Chan, X Zhang, Y Wang, S Yuan, J Zhou, RK Au-Yeung, KH Sze, D Yang, H Shuai, Y Hou, C Li, X Zhao, VK Poon, SP Leung, ML Yeung, J Yan, G Lu, DY Jin, GF Gao, JF Chan, KY Yuen Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells J. Biol. Chem., 2018-06-10;0(0):. 2018-06-10 [PMID: 29887526] (IHC-P, Neutralization, Western Blot, Human)
• CM Luo, N Wang, XL Yang, HZ Liu, W Zhang, B Li, B Hu, C Peng, QB Geng, GJ Zhu, F Li, ZL Shi Discovery of novel bat coronaviruses in south China that use the same receptor as MERS coronavirus J. Virol., 2018-06-13;0(0):. 2018-06-13 [PMID: 29669833] (Neutralization, Bat)
• W Li, RJG Hulswit, I Widjaja, VS Raj, R McBride, W Peng, W Widagdo, MA Tortorici, B van Dieren, Y Lang, JWM van Lent, JC Paulson, CAM de Haan, RJ de Groot, FJM van Kuppev, BL Haagmans, BJ Bosch Identification of sialic acid-binding function for the Middle East respiratory syndrome coronavirus spike glycoprotein Proc. Natl. Acad. Sci. U.S.A., 2017-09-18;0(0):. 2017-09-18 [PMID: 28923942] (Flow Cytometry, Human)
• W Mah, G Jiang, D Olver, C Gallant-Be, C Wiebe, DA Hart, L Koivisto, H Larjava, L Häkkinen Elevated CD26 Expression by Skin Fibroblasts Distinguishes a Profibrotic Phenotype Involved in Scar Formation Compared to Gingival Fibroblasts Am. J. Pathol., 2017-06-20;0(0):. 2017-06-20 [PMID: 28641076] (IHC, Human)
• E Angevin, N Isambert, V Trillet-Le, B You, J Alexandre, G Zalcman, P Vielh, F Farace, F Valleix, T Podoll, Y Kuramochi, I Miyashita, O Hosono, NH Dang, K Ohnuma, T Yamada, Y Kaneko, C Morimoto First-in-human phase 1 of YS110, a monoclonal antibody directed against CD26 in advanced CD26-expressing cancers Br. J. Cancer, 2017-03-14;116(9):1126-1134. 2017-03-14 [PMID: 28291776] (IHC, Human)
• Lessard J, Pelletier M, Biertho L, Biron S, Marceau S, Hould F, Lebel S, Moustarah F, Lescelleur O, Marceau P, Tchernof A Characterization of dedifferentiating human mature adipocytes from the visceral and subcutaneous fat compartments: fibroblast-activation protein alpha and dipeptidyl peptidase 4 as major components of matrix remodeling. PLoS ONE, 2015-03-27;10(3):e0122065. 2015-03-27 [PMID: 25816202] (Western Blot, Human)
• Ohnuma K, Hatano R, Aune T, Otsuka H, Iwata S, Dang N, Yamada T, Morimoto C Regulation of pulmonary graft-versus-host disease by IL-26+CD26+CD4 T lymphocytes. J Immunol, 2015-03-18;194(8):3697-712. 2015-03-18 [PMID: 25786689] (IHC-P, Mouse)
• Peck K, Cockrell A, Yount B, Scobey T, Baric R, Heise M Glycosylation of mouse DPP4 plays a role in inhibiting Middle East respiratory syndrome coronavirus infection. J Virol, 2015-02-04;89(8):4696-9. 2015-02-04 [PMID: 25653445] (IHC, Human)
• Scheuplein V, Seifried J, Malczyk A, Miller L, Hocker L, Vergara-Alert J, Dolnik O, Zielecki F, Becker B, Spreitzer I, Konig R, Becker S, Waibler Z, Muhlebach M High secretion of interferons by human plasmacytoid dendritic cells upon recognition of Middle East respiratory syndrome coronavirus. J Virol, 2015-01-21;89(7):3859-69. 2015-01-21 [PMID: 25609809] (Blocking, Human)
• Agrawal A, Garron T, Tao X, Peng B, Wakamiya M, Chan T, Couch R, Tseng C Generation of a transgenic mouse model of Middle East respiratory syndrome coronavirus infection and disease. J Virol, 2015-01-14;89(7):3659-70. 2015-01-14 [PMID: 25589660] (IHC-P, Western Blot, Human)
• Cai Y, Yu S, Postnikova E, Mazur S, Bernbaum J, Burk R, Zhang T, Radoshitzky S, Muller M, Jordan I, Bollinger L, Hensley L, Jahrling P, Kuhn J CD26/DPP4 cell-surface expression in bat cells correlates with bat cell susceptibility to Middle East respiratory syndrome coronavirus (MERS-CoV) infection and evolution of persistent infection. PLoS ONE, 2014-11-19;9(11):e112060. 2014-11-19 [PMID: 25409519] (Neutralization, Human)
• Weng , Matthias, Natarajan , Karthick, Scholz , Diana, Ivanova , Violeta, Sachinidis , Agapios, Hengstler , Jan G, Waldmann , Tanja, Leist , Marcel Lineage-specific regulation of epigenetic modifier genes in human liver and brain. PLoS ONE, 2014-07-23;9(7):e102035. 2014-07-23 [PMID: 25054330] (ICC, Human)
• Yamamoto , J, Ohnuma , K, Hatano , R, Okamoto , T, Komiya , E, Yamazaki , H, Iwata , S, Dang , N H, Aoe , K, Kishimoto , T, Yamada , T, Morimoto , C Regulation of somatostatin receptor 4-mediated cytostatic effects by CD26 in malignant pleural mesothelioma. Br J Cancer, 2014-04-17;110(9):2232-45. 2014-04-17 [PMID: 24743707] (ICC, IHC, Immunoprecipitation, Western Blot, Human)
• Eckerle I, Muller M, Kallies S, Gotthardt D, Drosten C In-vitro renal epithelial cell infection reveals a viral kidney tropism as a potential mechanism for acute renal failure during Middle East Respiratory Syndrome (MERS) Coronavirus infection. Virol J, 2013-12-23;10(0):359. 2013-12-23 [PMID: 24364985] (IHC, Human)
• Raj , V Stalin, Smits , Saskia L, Provacia , Lisette, van den Brand , Judith M, Wiersma , Lidewij, Ouwendijk , Werner J, Bestebroer , Theo M, Spronken , Monique, van Amerongen , Geert, Rottier , Peter J, Fouchier , Ron A M, Bosch , Berend J, Osterhaus , Albert D, Haagmans , Bart L Adenosine deaminase acts as a natural antagonist for dipeptidyl peptidase 4-mediated entry of the Middle East respiratory syndrome coronavirus. J Virol, 2013-11-20;88(3):1834-8. 2013-11-20 [PMID: 24257613] (Flow Cytometry, Ferret, Human)
• Havre , Pamela A, Dang , Long H, Ohnuma , Kei, Iwata , Satoshi, Morimoto , Chikao, Dang , Nam H CD26 expression on T-anaplastic large cell lymphoma (ALCL) line Karpas 299 is associated with increased expression of versican and MT1-MMP and enhanced adhesion. BMC Cancer, 2013-11-01;13(0):517. 2013-11-01 [PMID: 24180670] (Western Blot, Human)
• Yamaguchi U, Nakayama R, Honda K, Ichikawa H, Hasegawa T, Shitashige M, Ono M, Shoji A, Sakuma T, Kuwabara H, Shimada Y, Sasako M, Shimoda T, Kawai A, Hirohashi S, Yamada T Distinct gene expression-defined classes of gastrointestinal stromal tumor. J. Clin. Oncol., 2008-09-01;26(25):4100-8. 2008-09-01 [PMID: 18757323] (IHC-P, Human)
• Shin JW, Jurisic G, Detmar M Lymphatic-specific expression of dipeptidyl peptidase IV and its dual role in lymphatic endothelial function. Exp. Cell Res., 2008-08-03;314(16):3048-56. 2008-08-03 [PMID: 18708048] (IHC-Fr, Western Blot, Human)

Reviews for DPPIV/CD26 Antibody (AF1180) (2)

Average Rating: 4

(Based on 2 reviews)

We have 2 reviews tested in 1 species: Human.

reviewed by: Verified Customer IHC-Fr Human 02/22/2019

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: Frozen human skin sections
• Species: Human
• Lot: JOQ0218011

reviewed by: Anonymous - IHC-P Human 01/05/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID 23638030
• Species: Human

Bioinformatics

• Gene Symbol: DPP4
• Uniprot:
  • Q53TN1()