DPPIV/CD26 Antibody [Unconjugated]

Images

 
Western blot shows lysate of human peripheral blood mononuclear cells (PBMC). PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) ...read more
Western blot shows lysate of LoVo human colorectal adenocarcinoma cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) ...read more
Simple Western lane view shows lysates of LoVo human colorectal adenocarcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for DPPIV/CD26 at approximately 168 kDa (as indicated) using 2 µg/mL ...read more
DPPIV/CD26 was detected in immersion fixed paraffin-embedded sections of human psoriatic skin using Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) at 1 µg/mL overnight at 4 ...read more
DPPIV/CD26 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Goat Anti-Human DPPIV/CD26 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1180) at 1.7 µg/mL for 3 hours at ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, IHC, ICC/IF
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

DPPIV/CD26 Antibody [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human DPPIV/CD26 (Catalog # 1180-SE)
Asp34-Pro766
Accession # Q53TN1
Specificity
Detects human DPPIV/CD26 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse DPPIV is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DPP4
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.2 ug/mL
  • Simple Western 2 ug/mL
  • Immunohistochemistry 1-15 ug/mL
  • Immunocytochemistry 1-15 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
AF1180 in the following applications:

Publications
Read Publications using
AF1180 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DPPIV/CD26 Antibody [Unconjugated]

  • ADABP
  • ADCP-2
  • ADCP2DPP IV
  • Adenosine deaminase complexing protein 2TP103
  • CD26 antigen
  • CD26
  • CD26T-cell activation antigen CD26
  • dipeptidyl peptidase 4
  • Dipeptidyl peptidase IV
  • dipeptidylpeptidase 4
  • dipeptidyl-peptidase 4
  • dipeptidylpeptidase IV (CD26, adenosine deaminase complexing protein 2)
  • DPP4
  • DPPIV
  • EC 3.4.14.5

Background

DPPIV/CD26 (EC 3.4.14.5) is a serine exopeptidase that releases Xaa-Pro dipeptides from the N-terminus of oligo- and polypeptides. It is a type II membrane protein consisting of a short cytoplasmic tail, a transmembrane domain, and a long extracellular domain. The extracellular domain contains glycosylation sites, a cysteine-rich region and the catalytic active site (Ser, Asp and His charge relay system). The amino acid sequence of the mouse DPPIV/CD26 extracellular domain is 84% and 91% identical to the human and rat counterparts, respectively. In the native state, DPPIV/CD26 is present as a noncovalently linked homodimer on the cell surface of a variety of cell types. The soluble form is also detectable in human serum and other body fluids, the levels of which may have clinical significance in patients with cancer, liver and kidney diseases, and depression.

DPPIV/CD26 plays an important role in many biological and pathological processes. It functions as T cell-activating molecule (THAM). It serves as a cofactor for entry of HIV in CD4+ cells. It binds adenosine deaminase, the deficiency of which causes severe combined immunodeficiency disease in humans. It cleaves chemokines such as stromal-cell-derived factor 1 alpha and macrophage-derived chemokine. It degrades peptide hormones such as glucagon. It truncates procalcitonin, a marker for systemic bacterial infections with elevated levels detected in patients with thermal injury, sepsis and severe infection, and in children with bacterial meningitis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DPPIV/CD26 Antibody (AF1180)(18)

We have publications tested in 5 confirmed species: Human, Mouse, Camel, Ferret, Primate.

We have publications tested in 10 applications: Blocking, Flow, ICC, IF, IHC, IHC - paraffin, IHC Not Specified, IHC Paraffin-embedded, Immunprecipitation, WB.


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Blocking
(1)
Flow
(2)
ICC
(2)
IF
(1)
IHC
(3)
IHC - paraffin
(1)
IHC Not Specified
(1)
IHC Paraffin-embedded
(1)
Immunprecipitation
(1)
WB
(5)
All Applications
Filter By Species
Human
(13)
Mouse
(1)
Camel
(1)
Ferret
(1)
Primate
(1)
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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using AF1180 Applications Species
AS Cockrell, JC Johnson, IN Moore, DX Liu, KW Bock, MG Douglas, RL Graham, J Solomon, L Torzewski, C Bartos, R Hart, RS Baric, RF Johnson A spike-modified Middle East respiratory syndrome coronavirus (MERS-CoV) infectious clone elicits mild respiratory disease in infected rhesus macaques Sci Rep, 2018;8(1):10727. 2018 [PMID: 30013082] (IHC,, Primate) IHC Primate
AK Haverkamp, A Lehmbecker, I Spitzbarth, W Widagdo, BL Haagmans, J Segalés, J Vergara-Al, A Bensaid, JMA van den Br, ADME Osterhaus, W Baumgärtne Experimental infection of dromedaries with Middle East respiratory syndrome-Coronavirus is accompanied by massive ciliary loss and depletion of the cell surface receptor dipeptidyl peptidase 4 Sci Rep, 2018;8(1):9778. 2018 [PMID: 29950581] (ICC,, Camel) ICC Camel
H Chu, CM Chan, X Zhang, Y Wang, S Yuan, J Zhou, RK Au-Yeung, KH Sze, D Yang, H Shuai, Y Hou, C Li, X Zhao, VK Poon, SP Leung, ML Yeung, J Yan, G Lu, DY Jin, GF Gao, JF Chan, KY Yuen Middle East respiratory syndrome coronavirus and bat coronavirus HKU9 both can utilize GRP78 for attachment onto host cells J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29887526] (WB, ) WB
W Li, RJG Hulswit, I Widjaja, VS Raj, R McBride, W Peng, W Widagdo, MA Tortorici, B van Dieren, Y Lang, JWM van Lent, JC Paulson, CAM de Haan, RJ de Groot, FJM van Kuppev, BL Haagmans, BJ Bosch Identification of sialic acid-binding function for the Middle East respiratory syndrome coronavirus spike glycoprotein Proc. Natl. Acad. Sci. U.S.A., 2017;0(0):. 2017 [PMID: 28923942] (Flow,, Human) Flow Human
W Mah, G Jiang, D Olver, C Gallant-Be, C Wiebe, DA Hart, L Koivisto, H Larjava, L Häkkinen Elevated CD26 Expression by Skin Fibroblasts Distinguishes a Profibrotic Phenotype Involved in Scar Formation Compared to Gingival Fibroblasts Am. J. Pathol., 2017;0(0):. 2017 [PMID: 28641076] (IHC,, Human) IHC Human
E Angevin, N Isambert, V Trillet-Le, B You, J Alexandre, G Zalcman, P Vielh, F Farace, F Valleix, T Podoll, Y Kuramochi, I Miyashita, O Hosono, NH Dang, K Ohnuma, T Yamada, Y Kaneko, C Morimoto First-in-human phase 1 of YS110, a monoclonal antibody directed against CD26 in advanced CD26-expressing cancers Br. J. Cancer, 2017;116(9):1126-1134. 2017 [PMID: 28291776] (IHC,, Human) IHC Human
Lessard J, Pelletier M, Biertho L, Biron S, Marceau S, Hould F, Lebel S, Moustarah F, Lescelleur O, Marceau P, Tchernof A Characterization of dedifferentiating human mature adipocytes from the visceral and subcutaneous fat compartments: fibroblast-activation protein alpha and dipeptidyl peptidase 4 as major components of matrix remodeling. PLoS ONE, 2015;10(3):e0122065. 2015 [PMID: 25816202] (WB,, Human) WB Human
Ohnuma K, Hatano R, Aune T, Otsuka H, Iwata S, Dang N, Yamada T, Morimoto C Regulation of pulmonary graft-versus-host disease by IL-26+CD26+CD4 T lymphocytes. J Immunol, 2015;194(8):3697-712. 2015 [PMID: 25786689] (IHC - paraffin,, Mouse) IHC - paraffin Mouse
Peck K, Cockrell A, Yount B, Scobey T, Baric R, Heise M Glycosylation of mouse DPP4 plays a role in inhibiting Middle East respiratory syndrome coronavirus infection. J Virol, 2015;89(8):4696-9. 2015 [PMID: 25653445] (IHC Not Specified,, Human) IHC Not Specified Human
Scheuplein V, Seifried J, Malczyk A, Miller L, Hocker L, Vergara-Alert J, Dolnik O, Zielecki F, Becker B, Spreitzer I, Konig R, Becker S, Waibler Z, Muhlebach M High secretion of interferons by human plasmacytoid dendritic cells upon recognition of Middle East respiratory syndrome coronavirus. J Virol, 2015;89(7):3859-69. 2015 [PMID: 25609809] (Blocking,, Human) Blocking Human
Show All 18 Publications.

Review for DPPIV/CD26 Antibody (AF1180) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF1180:
Filter by Applications
IHC-P
(1)
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Filter by Species
Human
(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
IHC-P Human 01/05/2015
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Summary

ApplicationImmunohistochemistry-Paraffin
Sample TestedSee PMID 23638030
SpeciesHuman

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Recent Reviews

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Anonymous
01/05/2015
Application: IHC-P
Species: Human

Bioinformatics

Gene Symbol DPP4
Entrez
Uniprot