CXCL12/SDF-1 Antibodies: Novus Biologicals

MAB350

CXCL12/SDF-1 Antibody (79018) [Unconjugated]

(1 Review)

100 μg

MAB350

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder." class="big_lightbox">

CXCL12/SDF-1 was detected in immersion fixed paraffin-embedded sections of human breast tumor using Mouse Anti-Human CXCL12/SDF-1, Monoclonal Antibody (Catalog #MAB350) at 15ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # <A class=

DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder." alt="CXCL12/SDF-1 was detected in immersion fixed paraffin-embedded sections of human breast tumor using Mouse Anti-Human CXCL12/SDF-1, Monoclonal Antibody (Catalog #MAB350) at 15ug/mL at 37° Celsius for 4 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Mouse IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647MS) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm. Protocol available in COMET™ Panel Builder." class="big_thumb" />

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox">

CXCL12/SDF-1 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs; positive staining) and K562 human chronic myelogenous leukemia cell line (negative staining) using Mouse Anti-Human/Mouse CXCL12/SDF-1 Monoclonal Antibody (Catalog # MAB350) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # <a class=

NL007) and counterstained with DAPI (blue). Specific staining was localized to cell membranes. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in cripts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

CXCL12/SDF‑1 was detected in immersion fixed paraffin-embedded sections of human tonsil using 15 µg/mL Human/Mouse CXCL12/SDF‑1 Monoclonal Antibody (Catalog # MAB350) overnight at 4 °C. Tissue was stained with the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS002) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in cripts. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_lightbox">

CXCL12/SDF‑1 was detected in immersion fixed paraffin-embedded sections of human tonsil using Human/Mouse CXCL12/SDF‑1 Monoclonal Antibody (Catalog # MAB350) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # <a class=

CTS002) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections." class="big_thumb" />

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_lightbox">

HT1080 human fibrosarcoma cell line was stained with Mouse Anti-Human/Mouse CXCL12/SDF-1 Monoclonal Antibody (Catalog # MAB350, filled histogram) or isotype control antibody (Catalog # <a class=

MAB002, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3 Fixation & Permeabilization Buffer Kit (Catalog # FC012). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

VC001) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue)." class="big_lightbox">

<b>Orthogonal Strategies Validation. </b>CXCL12/SDF-1a mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope<sup>®</sup>Probe (Catalog # 422991) and stained using ACD RNAscope<sup>®</sup>2.5 HD Detection Reagents-Red (top image, Catalog # 32260). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Mouse Anti-Human CXCL12/SDF-1a Monoclonal Antibody (Catalog # MAB350) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # <a class=

VC001) and DAB chromogen (lower image, yellow-brown). Tissues were counterstained with hematoxylin (blue)." class="big_thumb" />

CXCR4 and CXCL12 expression and BM infiltration. (a) Expression analysis of CXCR4 and CXCL12 in uninfiltrated and infiltrated bone marrow specimens at the time of diagnosis by RQ-PCR. (b) Expression analysis of CXCR4 and CXCL12 in infiltrated bone marrow biopsies and the respective paired sample in patients under remission by RQ-PCR. (c) Representative immunohistochemical stains of CXCR4 (I–II) and CXCL12 (III–IV) on selected bone marrow specimens of DLBCL patients (magnification 20×). (I) and (III) represent the CXCR4 and CXCL12 staining of uninfiltrated bone marrow specimens, and (II) and (IV) represent those of the infiltrated bone marrow specimens. mRNA expression levels were calculated as a relative expression in comparison to uninfiltrated bone marrow specimens. Each bar represents the mean values of expression levels ± standard error of the mean (SEM). The comparison of the expression levels was performed by using the Mann–Whitney U-test or the Student’s t-test. All images were captured using an Olympus BX51 microscope and an Olympus E-330 camera. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31554271), licensed under a CC-BY license. Not internally tested by R&D Systems.

RhoA modulates the CXCR4-CXCL12 axis in breast tumors. (a) Representative immunohistochemical (IHC) staining images of breast primary tumor sections of mice orthotopically injected with 4T1 GFP-LUC cells carrying indicated lentiviral modifications, stained with antibodies for CD31 (top, left), F4/80 (middle, left) and alpha -SMA (bottom, left). CD31+ area (top, right), F4/80+ cells (middle, right) and alpha -SMA+ area (bottom, right) per tumor field of view (FOV) (mean ± SE) quantifications of IHC images. N = 4. (b) Representative IHC staining images of breast primary tumor sections of above mice stained with CXCL12 antibody (left). CXCL12+ area per tumor FOV (mean ± SE) quantification of IHC images. N = 4. (c) Representative immunofluorescent images of breast primary tumor sections of above mice co-stained with alpha -SMA, CXCL12 and DRAQ5 nuclear stain, showing the co-localization of alpha -SMA and CXCL12 signals. Scale bar: 50 µm. (d) Representative IHC staining images of breast primary tumor sections of above mice stained with CXCR4 antibody (left). CXCR4+ area per tumor FOV (mean ± SE) quantification of IHC images. N = 4. (e) Relative mCXCR4 mRNA levels normalized with mActin (mean ± SE), as quantified by qRT-PCR in primary breast tumors of above mice (top) and in 4T1 GFP-LUC cells carrying indicated lentiviral modifications (bottom). N = 4, ns- not significant *P < 0.05, unpaired Student’s t-test (two-tailed). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31705019), licensed under a CC-BY license. Not internally tested by R&D Systems.

CXCR4 and CXCL12 expression in DLBCL. (a) Expression analysis of CXCR4 and CXCL12 in non-neoplastic control germinal center B cells (GC-B) and diffuse large B cell lymphoma cells (DLBCL) consisting of DLBCL-NGCB and DLCBL-GCB, by RQ-PCR. GCB-DLBCL were further subdivided into primary (DLBCL-pGCB) and transformed DLBCL (DLBCL-pGCB) originating from follicular lymphoma. (b) Expression analysis of CXCR4 and CXCL12 in DLBCL samples with early (stage 1) and advanced stage (stage 2–4) (left graphs) and DLBCL samples with and without bone marrow infiltration (right graphs) by RQ-PCR. (c) Probability of 5-year-survival in DLBCL patients (our cohort left panel and the cohort of Lenz et al. right [20]) stratified by the third quartile of CXCR4 expression, respectively. (d) Representative immunohistochemical stains of CXCR4 (I–III) and CXCL12 (IV–VI) on DLBCL samples (magnification 20×). mRNA expression levels were calculated as a relative expression in comparison to the GC-B cells. All images were captured using an Olympus BX51 microscope and an Olympus E-330 camera. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31554271), licensed under a CC-BY license. Not internally tested by R&D Systems.

Tumor-promoting phenotype of the myeloid clusters.IHC staining demonstrating the expression of CD163, IL-6, IL-10, VEGF-A, MMP-9, SDF-1 and Bcl-xL by the myeloid cells associated with anthracosis. For each protein, representative images showing positive and negative staining areas were selected from the same slide. The quantification was performed by analyzing random images of myeloid cluster areas associated with anthracosis and other areas (10 images for each group) from 10 patients. Two-tailed Student's t-test was used for statistical analysis. Shown are means ± SEM, *** P<0.001. (Scale bar, 200 µm for 100× and 400×; 25 µm for 1600×) Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/23717691), licensed under a CC-BY license. Not internally tested by R&D Systems.

Afferent lymph vessels in chronically inflamed skin express CXCL12.Immunofluorescence staining of frozen sections of chronically inflamed skin (21 days after inflammation elicited by subcutaneous injection of CFA) for CXC12 and CCL21 by LYVE-1+ lymph vessels using DAPI counterstaining. One representative image of lymph vessels analyzed in 5 mice is shown. Scale bars, 10 µm. Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/24752354), licensed under a CC-BY license. Not internally tested by R&D Systems.

CXCR4/CXCL12 signaling promotes lymphangiogenesis in TSCC. a Cell proliferation of human lymphatic endothelial cells (HLECs) treated with CXCL12 (100 ng/ml) or vehicle control, assessed by CCK-8 assay. b Wound healing assay showing the effect of CXCL12 on HLEC migration. Representative images (left) and quantification (right). Scale bars, 100 μm. c Tube formation assay of HLECs treated with CXCL12 (100 ng/ml) or vehicle control. Representative images (left) and quantification (right). Scale bars, 100 μm. d Western blot analysis of lymphangiogenic factors (VEGF-C, VEGFR-3, and Prox1) in HLECs treated with CXCL12 (100 ng/ml) or vehicle control. Representative blots (left) and quantification (right). e Western blot analysis of PI3K, p-AKT, and AKT in HLECs treated with CXCL12 (100 ng/ml) or vehicle control. Representative blots (left) and quantification (right). f Tube formation assay of HLECs treated with CXCL12 (100 ng/ml), LY294002 (20 μM), or CXCL12 + LY294002. Representative images (left) and quantification (right). Scale bars, 100 μm. g Schematic representation of the co-culture system used to investigate the interaction between TSCC cells and HLECs. h Transwell migration assay of HLECs co-cultured with CAL-27 cells with different CXCR4 expression levels. Representative images (left) and quantification (right). Scale bars, 100 μm. Data are presented as mean ± SD from three independent experiments. ***p < 0.001, ****p < 0.0001(compared to Control); ####p < 0.0001(compared to CXCL12) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40629333), licensed under a CC-BY license. Not internally tested by R&D Systems.

CXCL12 neutralization improves alopecia areata. (A) In this experimental design, skin-draining lymph node (SDLN) cells were isolated from AA-affected C3H/HeN female mice and intradermally injected into the dorsal skin of naïve mice to induce AA. Severe hair loss was observed after 12 weeks, and alpha CXCL12 (20 μg) was subcutaneously injected twice in a 2-week interval. (B) Skin sections from normal and AA mice were stained with an anti-CXCL12 antibody (green). The expression of CXCL12 increased in the AA model. The scale bar is set at 500 μm. (C) The administration of alpha CXCL12 in AA mice significantly reduced the areas of hair loss, which are marked in the images with dotted lines. The extent of hair loss in both the control and alpha CXCL12-treated mice was quantified using Image J (v1.53t). *** p < 0.001 vs. control, n = 3. (D) Skin sections of AA and alpha CXCL12-treated mice were stained with anti-CD8, anti-MHC-I, or anti-MHC-II antibodies. The alpha CXCL12-treated group had reduced expression of these immune reaction markers (green; white arrows). DAPI staining (blue) indicates cell nuclei. The scale bar is set at 100 μm. An asterisk indicates a statistical difference using Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/25/3/1705), licensed under a CC-BY license. Not internally tested by R&D Systems.

Transcriptomic analysis of CXCR4/CXCL12-regulated gene expression in TSCC. a Principal component analysis (PCA) of RNA-seq data from CAL-27 cells with different CXCR4 expression levels (Control, CXCR4-KD, and CXCR4-OE). b Volcano plot showing differentially expressed genes (DEGs) between Control and CXCR4-KD CAL-27 cells. c Volcano plot showing DEGs between Control and CXCR4-OE CAL-27 cells. d Heatmap of DEGs between Control and CXCR4-KD CAL-27 cells. e Heatmap of DEGs between Control and CXCR4-OE CAL-27 cells. f qRT-PCR validation of selected DEGs (PI3K3CA, AKT1, PTEN, CXCL12, and CXCR4) in CAL-27 cells with different CXCR4 expression levels. g Western blot validation of selected DEGs in CAL-27 cells with different CXCR4 expression levels. Representative blots (left) and quantification (right). h Proposed mechanistic model illustrating how the CXCR4/CXCL12 axis promotes TSCC lymphatic metastasis through the PI3K/AKT pathway. Data are presented as mean ± SD from three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (compared to Control); ##p < 0.01, ###p < 0.001, ####p < 0.0001(compared to CXCR4-OE) Image collected and cropped by CiteAb from the following open publication (//pubmed.ncbi.nlm.nih.gov/40629333), licensed under a CC-BY license. Not internally tested by R&D Systems.

Neutralization of CXCL12 promotes hair growth in a testosterone-induced AGA model. (A) The back skin of 7-week-old C3H male mice was shaved and subcutaneous (s.c.) injections of testosterone propionate (TP) were used to induce the AGA. (B) The expression of CXCL12 in the dorsal skin of control and TP-treated mice was detected using immunofluorescence. CXCL12+ cells (red) are indicated by white arrows, and DAPI staining (blue) indicates cell nuclei. The scale bar is set at 100 μm. (C) Different doses of CXCL12 neutralizing antibody ( alpha CXCL12, 5, and 20 μg) were subcutaneously injected twice a week for three weeks. A daily topical treatment of 0.05% dutasteride served as a positive control. alpha CXCL12 administration accelerated hair growth in TP-treated C3H mice and the hair weight was measured. ### p < 0.001 vs. control, * p < 0.05, ** p < 0.01 vs. TP-treated, n = 7. (D) alpha CXCL12 (10, 100, or 1000 ng/mL) treatment increased the length of mouse vibrissa follicles in the AGA mimic ex vivo model. ## p < 0.01 vs. control, * p < 0.05 vs. TP-treated, n = 8. + or -: treated with or without TP, alpha CXCL12. The asterisk and sharp symbols indicate statistical differences using Student’s t-test. Image collected and cropped by CiteAb from the following open publication (//www.mdpi.com/1422-0067/25/3/1705), licensed under a CC-BY license. Not internally tested by R&D Systems.

350-NS) was serially diluted and captured by Mouse Anti-Human/Mouse CXCL12/SDF‑1 Monoclonal Antibody (Catalog # MAB350) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL12/SDF‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-310-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (Catalog # <a class=

350-NS) was serially diluted and captured by Mouse Anti-Human/Mouse CXCL12/SDF‑1 Monoclonal Antibody (Catalog # MAB350) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL12/SDF‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-310-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

460-SD) was serially diluted and captured by Mouse Anti-Human/Mouse CXCL12/SDF‑1 Monoclonal Antibody (Catalog # MAB350) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL12/SDF‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-310-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

Recombinant Mouse CXCL12/SDF‑1 alpha (Catalog # <a class=

460-SD) was serially diluted and captured by Mouse Anti-Human/Mouse CXCL12/SDF‑1 Monoclonal Antibody (Catalog # MAB350) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL12/SDF‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-310-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

, , , , , ,

Applications: , , , , , ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Validated by:

Validated by:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB350U-100UG
Alexa Fluor 405	FAB350V-100UG
Alexa Fluor 488	IC350G-100
Alexa Fluor 488	FAB350G-100UG
Alexa Fluor 532	FAB350X
Alexa Fluor 594	FAB350T-100UG
Alexa Fluor 647	FAB350R-100UG
Alexa Fluor 700	FAB350N-100UG
Alexa Fluor 750	FAB350S-100UG
Alexa Fluor Plus 405	FAB350AFP405-100UG
Alexa Fluor Plus 488	FAB350AFP488-100UG
Alexa Fluor Plus 555	FAB350AFP555-100UG
Alexa Fluor Plus 594	FAB350AFP594-100UG
Alexa Fluor Plus 647	FAB350AFP647-100UG
Alexa Fluor Plus 680	FAB350AFP680-100UG
Allophycocyanin	IC350A
Biotin	FAB350B
CoraFluor 1	FAB350CL1
DyLight 350	FAB350D
DyLight 405	FAB350E
DyLight 488	FAB350K
DyLight 550	FAB350L
DyLight 594	FAB350M
DyLight 650	FAB350W
DyLight 680	FAB350Y
DyLight 755	FAB350Z
Fluorescein	IC350F
HRP	FAB350H
Janelia Fluor 525	FAB350JF525
Janelia Fluor 549	FAB350I
Janelia Fluor 646	FAB350J
Janelia Fluor 669	FAB350JF669
PerCP (Peridinin-chlorophyll Protein Complex)	IC350C
Phycoerythrin	IC350P
mFluor Violet 450 SE	FAB350MFV450
mFluor Violet 500 SE	FAB350MFV500
mFluor Violet 610 SE	FAB350MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	IC350C
Carrier Free	IC350F
Carrier Free	IC350P
Carrier Free	IC350A
Carrier Free	MAB350-SP
Carrier Free	MAB350-500
Carrier Free	IC350G-100
Carrier Free	FAB350V-100UG
Carrier Free	FAB350U-100UG
Carrier Free	FAB350T-100UG
Carrier Free	FAB350S-100UG
Carrier Free	FAB350R-100UG
Carrier Free	FAB350N-100UG
Carrier Free	FAB350G-100UG

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MAB310

CXCL12/SDF-1 Antibody (79014) [Unconjugated]

(69 Publications)

100 μg

MAB310

350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Human/Feline/ Rhesus Macaque CXCL12/ SDF-1a (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse CXCL12/SDF-1 Monoclonal Antibody (Catalog # MAB310). At 111 µg/mL, this antibody will neutralize > 50% of the chemotactic effect." class="big_lightbox">

Recombinant Human/Feline/ Rhesus Macaque CXCL12/ SDF-1a (Catalog # <a class=

350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Human/Feline/ Rhesus Macaque CXCL12/ SDF-1a (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse CXCL12/SDF-1 Monoclonal Antibody (Catalog # MAB310). At 111 µg/mL, this antibody will neutralize > 50% of the chemotactic effect." alt="Recombinant Human/Feline/ Rhesus Macaque CXCL12/ SDF-1a (Catalog # 350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). Chemotaxis elicited by Recombinant Human/Feline/ Rhesus Macaque CXCL12/ SDF-1a (2 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human/Mouse CXCL12/SDF-1 Monoclonal Antibody (Catalog # MAB310). At 111 µg/mL, this antibody will neutralize > 50% of the chemotactic effect." class="big_thumb" />

Enumeration of bone marrow and blood Lin−/Sca1+ cell numbers following stroke and SDF1-A Antibody.(A) Twenty-four hour post stroke+SDF1-A antibody mice show a marked increase in the number of Lin−/Sca1+ cells present in the bone marrow compared to controls. (B) Twenty-four hour post stroke mice showed a marked decrease in the mobilization of these cells to the blood. * P<0.05. Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24465621), licensed under a CC-BY license. Not internally tested by R&D Systems.

Administration of Lin−/Sca1+ cells post stroke.A. Administration of Lin−/Sca1+ cells post stroke significantly reduced infarct volume at 24 hours post stroke (*P<0.05). B. Administration of the Lin−/Sca1+ cells with an SDF1-A antibody (AB) no longer demonstrates a beneficial reduction in the infarct volume (p = NS). Image collected and cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/24465621), licensed under a CC-BY license. Not internally tested by R&D Systems.

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	FAB310U-100UG
Alexa Fluor 405	FAB310V-100UG
Alexa Fluor 488	FAB310G-100UG
Alexa Fluor 532	FAB310X-100UG
Alexa Fluor 594	FAB310T-100UG
Alexa Fluor 647	FAB310R-100UG
Alexa Fluor 700	FAB310N-100UG
Alexa Fluor 750	FAB310S-100UG
Alexa Fluor Plus 405	FAB310AFP405-100UG
Alexa Fluor Plus 488	FAB310AFP488-100UG
Alexa Fluor Plus 555	FAB310AFP555-100UG
Alexa Fluor Plus 594	FAB310AFP594-100UG
Alexa Fluor Plus 647	FAB310AFP647-100UG
Alexa Fluor Plus 680	FAB310AFP680-100UG
Allophycocyanin	FAB310A
Biotin	FAB310B
CoraFluor 1	FAB310CL1
DyLight 350	FAB310D
DyLight 405	FAB310E
DyLight 488	FAB310K
DyLight 550	FAB310L
DyLight 594	FAB310M
DyLight 650	FAB310W
DyLight 680	FAB310Y
DyLight 755	FAB310Z
FITC	FAB310F
HRP	FAB310H
Janelia Fluor 525	FAB310JF525
Janelia Fluor 549	FAB310I
Janelia Fluor 646	FAB310J
Janelia Fluor 669	FAB310JF669
PE	FAB310P
PerCP	FAB310C
mFluor Violet 450 SE	FAB310MFV450
mFluor Violet 500 SE	FAB310MFV500
mFluor Violet 610 SE	FAB310MFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	MAB310-SP
Carrier Free	MAB310-500

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AF-310-NA

CXCL12/SDF-1 Antibody [Unconjugated]

(2 Reviews)

(25 Publications)

100 μg

AF-310-NA

350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL12/SDF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-310-NA). The ND₅₀ is typically 1.5-6.0 µg/mL." class="big_lightbox">

350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL12/SDF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-310-NA). The ND₅₀ is typically 1.5-6.0 µg/mL." alt="Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (Catalog # 350-NS) chemoattracts the BaF3 mouse pro-B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1a (2 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CXCL12/SDF-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-310-NA). The ND₅₀ is typically 1.5-6.0 µg/mL." class="big_thumb" />

350-NS) was serially diluted and captured by Mouse Anti-Human/Mouse CXCL12/SDF‑1 Monoclonal Antibody (Catalog # MAB350) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL12/SDF‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-310-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

350-NS) was serially diluted and captured by Mouse Anti-Human/Mouse CXCL12/SDF‑1 Monoclonal Antibody (Catalog # MAB350) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL12/SDF‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-310-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

460-SD) was serially diluted and captured by Mouse Anti-Human/Mouse CXCL12/SDF‑1 Monoclonal Antibody (Catalog # MAB350) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL12/SDF‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-310-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox">

460-SD) was serially diluted and captured by Mouse Anti-Human/Mouse CXCL12/SDF‑1 Monoclonal Antibody (Catalog # MAB350) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CXCL12/SDF‑1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-310-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Goat Polyclonal

Host: Goat Polyclonal

Sizes:

Sample Size Available

Sizes: Sample Size Available

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	AF-310-NAU-100UG
Alexa Fluor 405	AF-310-NAV-100UG
Alexa Fluor 488	AF-310-NAG-100UG
Alexa Fluor 532	AF-310-NAX-100UG
Alexa Fluor 594	AF-310-NAT-100UG
Alexa Fluor 647	AF-310-NAR-100UG
Alexa Fluor 700	AF-310-NAN-100UG
Alexa Fluor 750	AF-310-NAS-100UG
Alexa Fluor Plus 405	AF-310-NAAFP405
Alexa Fluor Plus 488	AF-310-NAAFP488
Alexa Fluor Plus 555	AF-310-NAAFP555
Alexa Fluor Plus 594	AF-310-NAAFP594
Alexa Fluor Plus 647	AF-310-NAAFP647
Alexa Fluor Plus 680	AF-310-NAAFP680
Allophycocyanin	AF-310-NAA
Biotin	BAF310
CoraFluor 1	AF-310-NACL1
DyLight 350	AF-310-NAD
DyLight 405	AF-310-NAE
DyLight 488	AF-310-NAK
DyLight 550	AF-310-NAL
DyLight 594	AF-310-NAM
DyLight 650	AF-310-NAW
DyLight 680	AF-310-NAY
DyLight 755	AF-310-NAZ
FITC	AF-310-NAF
HRP	AF-310-NAH
Janelia Fluor 525	AF-310-NAJF525
Janelia Fluor 549	AF-310-NAI
Janelia Fluor 646	AF-310-NAJ
Janelia Fluor 669	AF-310-NAJF669
PE	AF-310-NAP
PerCP	AF-310-NAC
mFluor Violet 450 SE	AF-310-NAMFV450
mFluor Violet 500 SE	AF-310-NAMFV500
mFluor Violet 610 SE	AF-310-NAMFV610

Available Formulations

Formulation	Catalog #	Availability	Price
Carrier Free	AF-310-SP

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NBP3-41072

CXCL12/SDF-1 Antibody (C3) - BSA Free

100 ul

NBP3-41072

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

,

Applications: ,

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP3-41081

CXCL12/SDF-1 Antibody (C4) - BSA Free

100 ul

NBP3-41081

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP3-41080

CXCL12/SDF-1 Antibody (C2) - BSA Free

100 ul

NBP3-41080

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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NBP3-28766

CXCL12/SDF-1 Antibody (anti-CXCL12) - Humanized - Low Endotoxin, Azide and BSA Free

100 μg

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

, ,

Applications: , ,

Host:

CHO Monoclonal

Host: CHO Monoclonal

Conjugate:

Unconjugated

Available Conjugates:

Conjugate	Catalog #	Availability	Size	Price
Alexa Fluor 350	NBP3-28766AF350
Alexa Fluor 405	NBP3-28766AF405
Alexa Fluor 488	NBP3-28766AF488
Alexa Fluor 532	NBP3-28766AF532
Alexa Fluor 594	NBP3-28766AF594
Alexa Fluor 647	NBP3-28766AF647
Alexa Fluor 700	NBP3-28766AF700
Alexa Fluor 750	NBP3-28766AF750
Alexa Fluor Plus 405	NBP3-28766AFP405
Alexa Fluor Plus 488	NBP3-28766AFP488
Alexa Fluor Plus 555	NBP3-28766AFP555
Alexa Fluor Plus 594	NBP3-28766AFP594
Alexa Fluor Plus 647	NBP3-28766AFP647
Alexa Fluor Plus 680	NBP3-28766AFP680
Allophycocyanin	NBP3-28766APC
Biotin	NBP3-28766B
CoraFluor 1	NBP3-28766CL1
DyLight 350	NBP3-28766UV
DyLight 405	NBP3-28766V
DyLight 488	NBP3-28766G
DyLight 550	NBP3-28766R
DyLight 594	NBP3-28766DL594
DyLight 650	NBP3-28766C
DyLight 680	NBP3-28766FR
DyLight 755	NBP3-28766IR
FITC	NBP3-28766F
HRP	NBP3-28766H
Janelia Fluor 525	NBP3-28766JF525
Janelia Fluor 549	NBP3-28766JF549
Janelia Fluor 646	NBP3-28766JF646
Janelia Fluor 669	NBP3-28766JF669
PE	NBP3-28766PE
PerCP	NBP3-28766PCP
mFluor Violet 450 SE	NBP3-28766MFV450
mFluor Violet 500 SE	NBP3-28766MFV500
mFluor Violet 610 SE	NBP3-28766MFV610

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H00006387-M01

CXCL12/SDF-1 Antibody (1E5) - Azide and BSA Free

0.1 mg

H00006387-M01

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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H00006387-M03

CXCL12/SDF-1 Antibody (1F10) - Azide and BSA Free

0.1 mg

H00006387-M03

Reactivity:

Hu

Species Glossary

Reactivity: Hu

Applications:

Applications:

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Unconjugated

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FAB350AFP680

CXCL12/SDF-1 Antibody (79018) [Alexa Fluor™ Plus 680]

100 μg

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Alexa Fluor Plus 680

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FAB310AFP647

CXCL12/SDF-1 Antibody (79014) [Alexa Fluor™ Plus 647]

100 μg

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Alexa Fluor Plus 647

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FAB310AFP680

CXCL12/SDF-1 Antibody (79014) [Alexa Fluor™ Plus 680]

100 μg

Reactivity:

Hu, Mu

Species Glossary

Reactivity: Hu, Mu

Host:

Mouse Monoclonal

Host: Mouse Monoclonal

Conjugate:

Alexa Fluor Plus 680

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At	=	A. thaliana	SyHa	=	Golden Syrian Hamster
All	=	All Species	Ha	=	Hamster
An	=	Animal	Hu	=	Human
ArHa	=	Armenian Hamster	I	=	Insect
Av	=	Avian	Ll	=	Llama
Bb	=	Baboon	Ma	=	Mammal
Ba	=	Bacteria	Mk	=	Monkey
Bv	=	Bovine	Mu	=	Mouse
Ca	=	Canine	Multi	=	Multi-species
Ce	=	C. Elegans	NA	=	Non-species specific
Ch	=	Chicken	Or	=	Orangutan
ChHa	=	Chinese Hamster	Pl	=	Plant
Cm	=	Cynomolgus Monkey	Pm	=	Primate
Do	=	Donkey	Po	=	Porcine
Dr	=	Drosophilia	Rb	=	Rabbit
Ec	=	E. Coli	Rt	=	Rat
Eq	=	Equine	RM	=	Rhesus Macaque
Fe	=	Feline	Sh	=	Sheep
Ft	=	Ferret	Vb	=	Vertebrate
Fi	=	Fish	Vi	=	Virus
Fu	=	Fungi	Xp	=	Xenopus
Ge	=	Gerbil	Ye	=	Yeast
GP	=	Guinea Pig	Ze	=	Zebrafish
Gt	=	Goat

Category

Protein/Target

Applications

Reactivity

Citation

Reviews

Host

Available Conjugates

Format

Modification

Clonality

Clone

Images Type

Isotype

Preservative

Research Areas

Has Sample Size

Promotional Item

Species Glossary

CXCL12/SDF-1 Antibodies

CXCL12/SDF-1 Antibody (79018) [Unconjugated]

CXCL12/SDF-1 Antibody (79014) [Unconjugated]

CXCL12/SDF-1 Antibody [Unconjugated]

CXCL12/SDF-1 Antibody (C3) - BSA Free

CXCL12/SDF-1 Antibody (C4) - BSA Free

CXCL12/SDF-1 Antibody (C2) - BSA Free

CXCL12/SDF-1 Antibody (anti-CXCL12) - Humanized - Low Endotoxin, Azide and BSA Free

CXCL12/SDF-1 Antibody (1E5) - Azide and BSA Free

CXCL12/SDF-1 Antibody (1F10) - Azide and BSA Free

CXCL12/SDF-1 Antibody (79018) [Alexa Fluor™ Plus 680]

CXCL12/SDF-1 Antibody (79014) [Alexa Fluor™ Plus 647]

CXCL12/SDF-1 Antibody (79014) [Alexa Fluor™ Plus 680]