CCR2 Antibody (48607) [Unconjugated]

Images

 
Human peripheral blood monocytes were stained with Mouse Anti-Human CD14 PE‑conjugated Monoclonal Antibody (Catalog # FAB3832P) and either (A) Mouse Anti-Human CCR2 Monoclonal Antibody (Catalog # MAB150) or ...read more
CCR2 was detected in immersion fixed paraffin-embedded sections of human tonsil using Mouse Anti-Human CCR2 Monoclonal Antibody (Catalog # MAB150) at 15 µg/mL overnight at 4 °C. Tissue was stained using the ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Flow, IHC, CyTOF-ready
Clone
48607
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

CCR2 Antibody (48607) [Unconjugated] Summary

Additional Information
Clone 48607 was used by HLDA to establish CD designation
Immunogen
NS0 mouse myeloma cell line transfected with human CCR2
Met1-Leu360
Accession # NP_001116868
Specificity
Detects human CCR-2 transfectants but not the parental cell line or CCR-5 transfectants.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
CCR2
Purity Statement
Protein A or G purified from ascites
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunohistochemistry 8-50 ug/mL
Publications
Read Publications using
MAB150 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CCR2 Antibody (48607) [Unconjugated]

  • C-C chemokine receptor type 2
  • C-C CKR-2
  • CC-CKR-2
  • CC-CKR-2CKR2B
  • CCR2
  • CCR-2
  • CCR2A
  • CCR2B
  • CD192 antigen
  • CD192
  • chemokine (C-C motif) receptor 2
  • CKR2
  • CKR2A
  • CMKBR2
  • CMKBR2MGC111760
  • FLJ78302
  • MCP-1 receptor
  • MCP-1-R
  • MCP-1-RMGC103828
  • MGC168006
  • Monocyte chemoattractant protein 1 receptor
  • monocyte chemotactic protein 1 receptor

Background

CCR2 is a G-protein linked seven transmembrane domain spanning chemokine receptor that preferentially binds monocyte chemoattractant proteins-1 and -3 (MCP-1 and MCP-3). Two isoforms of this receptor (CCR2A and CCR2B) are expressed on cell surfaces as a result of alternate splicing from the same gene. These two CCR2 variants differ only at their intracellular carboxyl terminals, with the CCR2A form possessing 14 additional amino acids. This may provide a mechanism by which cells responding to similar extracellular ligands can activate different intracellular second messengers. Cells that respond to the action of MCP-1 and therefore are likely to express CCR2 receptors, include monocytes, T cells, NK cells, basophils, mast cells and dendritic cells. A recent report suggests that B cells may also express CCR2 receptors. The recognition that a variety of chemokine receptors, including CCR2, can serve as HIV fusion co-factors and as facilitators of T cell recruitment during inflammation makes chemokine receptor monitoring an important exercise in elucidating the HIV infection process and the regulation of inflammatory reactions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CCR2 Antibody (MAB150)(35)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Primate - Cholrocebus pygerythrus (Vervet Monkey).

We have publications tested in 6 applications: Cell Culture, Flow Cytometry, ICC, IHC, IHC-P, Neutralization.


Filter By Application
Cell Culture
(1)
Flow Cytometry
(23)
ICC
(4)
IHC
(2)
IHC-P
(3)
Neutralization
(5)
All Applications
Filter By Species
Human
(32)
Mouse
(1)
Rat
(1)
Primate - Cholrocebus pygerythrus (Vervet Monkey)
(1)
All Species
Showing Publications 1 - 10 of 35. Show All 35 Publications.
Publications using MAB150 Applications Species
Bueto-Beni, C;Tafuni, A;Chelko, SP;Sheppard, MN;Field, E;Tollit, J;Heenan, IK;Barnes, A;Taylor, MR;Mestroni, L;Kaski, JP;Saffitz, JE;Asimaki, A; Innate Immune Signaling in Hearts and Buccal Mucosa Cells of Patients with Arrhythmogenic Cardiomyopathy bioRxiv : the preprint server for biology 2023-07-27 [PMID: 37649905] (IHC, Human) IHC Human
L Pradhan, D Moore, EM Ovadia, SL Swedzinski, T Cossette, RA Sikes, K van Golen, AM Kloxin Dynamic bioinspired coculture model for probing ER+ breast cancer dormancy in the bone marrow niche Science Advances, 2023-03-08;9(10):eade3186. 2023-03-08 [PMID: 36888709] (Neutralization, Human) Neutralization Human
WB Fang, D Sofia Acev, C Smart, B Zinda, N Alissa, K Warren, G Fraga, LC Huang, Y Shyr, W Li, L Xie, V Staggs, Y Hong, F Behbod, N Cheng Expression of CCL2/CCR2 signaling proteins in breast carcinoma cells is associated with invasive progression Scientific Reports, 2021-04-22;11(1):8708. 2021-04-22 [PMID: 33888841] (Cell Culture, Human) Cell Culture Human
LM Petti, SA Marlatt, Y Luo, EH Scheideman, A Shelar, D DiMaio Regulation of C-C chemokine receptor 5 (CCR5) stability by Lys-197 and by transmembrane protein aptamers that target it for lysosomal degradation J. Biol. Chem., 2018-04-20;0(0):. 2018-04-20 [PMID: 29678881] (Flow Cytometry) Flow Cytometry
G Sebastiani, G Ventriglia, A Stabilini, C Socci, C Morsiani, A Laurenzi, L Nigi, C Formichi, B Mfarrej, A Petrelli, G Fousteri, TM Brusko, F Dotta, M Battaglia Regulatory T-cells from pancreatic lymphnodes of patients with type-1 diabetes express increased levels of microRNA miR-125a-5p that limits CCR2 expression Sci Rep, 2017-07-31;7(1):6897. 2017-07-31 [PMID: 28761107] (Flow Cytometry, Human) Flow Cytometry Human
J Przybyl, M Kowalewska, A Quattrone, B Dewaele, V Vanspauwen, S Varma, S Vennam, AM Newman, M Swierniak, E Baku?a-Zal, JA Siedlecki, M Bidzinski, J Cools, M van de Rij, M Debiec-Ryc Macrophage infiltration and genetic landscape of undifferentiated uterine sarcomas JCI Insight, 2017-06-02;2(11):. 2017-06-02 [PMID: 28570276] (IHC, Human) IHC Human
JC Nieto, C Zamora, E Cantó, E Garcia-Pla, J Gordillo, MA Ortiz, C Juárez, S Vidal CSF-1 regulates the function of monocytes in Crohn's disease patients in remission Sci Rep, 2017-03-07;7(1):92. 2017-03-07 [PMID: 28273887] (Flow Cytometry, Human) Flow Cytometry Human
Zaidoon Al-Jaderi Glatiramer Acetate, Dimethyl Fumarate, and Monomethyl Fumarate Upregulate the Expression of CCR10 on the Surface of Natural Killer Cells and Enhance Their Chemotaxis and Cytotoxicity Front Immunol, 2016-10-19;7(0):437. 2016-10-19 [PMID: 27807435] (Flow Cytometry, Human) Flow Cytometry Human
Evidence for chemokine synergy during neutrophil migration in ARDS Thorax, 2016-08-05;0(0):. 2016-08-05 [PMID: 27496101] (Flow Cytometry, Human) Flow Cytometry Human
Williams D, Anastos K, Morgello S, Berman J JAM-A and ALCAM are therapeutic targets to inhibit diapedesis across the BBB of CD14+CD16+ monocytes in HIV-infected individuals. J Leukoc Biol, 2014-11-24;97(2):401-12. 2014-11-24 [PMID: 25420915] (Flow Cytometry, Human) Flow Cytometry Human
Show All 35 Publications.

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Blogs on CCR2.

Rest in Peace: Is the Receptor Interacting Protein (RIP) Kinase-3 (RIPK3) a Protector or Offender?
By Jamshed Arslan, Pharm. D., PhD. Receptor interacting protein kinases (RIPKs) in necroptosisDeath is perhaps inevitable. Cell death can be programed and immunologically silent (apoptosis), unprogrammed and inflamm...  Read full blog post.

CCR2 or CD192
CCR2 is a receptor for several monocyte chemoattractant proteins (MCP1, MCP3, MCP4) that specifically govern monocyte chemotaxis. CCR2 transduces its downstream signals through increasing intracellular calcium ion levels. For example, MCP1 regulate...  Read full blog post.

CCR2: Affecting Autoimmunity via MCP1 interactions
CCR2, also known as CD192 (cluster of differentiation 192), is a chemokine receptor and is expressed by monocytes, activated T cells, B cells and natural killer cells. This protein is encoded by CCR2 gene in humans. CCR2 gene encodes two protein isofo...  Read full blog post.

MCP1: One Chemoattractant that's Hard to Resist
Monocyte Chemoattractant Protein (MCP1) is a potent monocyte attractant, is a member of the CC chemokine subfamily. MCP1 exerts its effects through binding to G-protein-coupled receptors on the surface of leukocytes targeted for activation and migrati...  Read full blog post.

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Bioinformatics

Gene Symbol CCR2
Uniprot