Rat CXCL12/SDF-1 ELISA Kit (Colorimetric)

ELISA: Rat CXCL12/SDF-1 ELISA Kit (Colorimetric) [NBP3-11823] - Samples were spiked with high concentrations of Rat CXCL12/SDF-1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Rat CXCL12/SDF-1 ELISA Kit (Colorimetric) [NBP3-11823] - Standard Curve Reference

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL

Rat CXCL12/SDF-1 ELISA Kit (Colorimetric) Summary

• Description: This ELISA kit applies to the in vitro quantitative determination of Rat SDF-1/CXCL12 concentrations in serum, plasma and other biological fluids.
• Specificity: This kit recognizes Rat SDF-1/CXCL12 in samples. No significant cross-reactivity or interference between Rat SDF-1/CXCL12 and analogues was observed.
• Standard Curve Range: 0.31 - 20 ng/mL
• Sensitivity: 0.19 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: %CV < 5.38
• Intra-Assay: %CV < 5.62
• Spike Recovery: 83 - 106%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: CXCL12

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat CXCL12/SDF-1 ELISA Kit (Colorimetric)

• chemokine (C-X-C motif) ligand 12
• C-X-C motif chemokine 12
• CXCL12
• hIRH
• hSDF-1
• Intercrine reduced in hepatomas
• IRH
• PBSF
• PBSFSDF-1a
• Pre-B cell growth-stimulating factor
• SCYB12
• SDF1
• SDF-1
• SDF1A
• SDF1B
• SDF1SDF-1b
• stromal cell-derived factor 1
• TLSF
• TLSF-a
• TLSF-b
• TPAR1

Background

The C-X-C or alpha chemokine family is characterized by a pair of cysteine residues separated by a single amino acid and primarily functions as chemoattractants for neutrophils. The C-X-C family includes IL-8, NAP-2, MSGA and stromal cell-derived factor-1, or SDF-1. SDF-1 was originally described as a pre-B cell stimulatory factor, but has now been shown to function as a potent chemoattractant for T cells and monocytes, but not neutrophils. Receptors for the C-X-C family are G protein-coupled, seven-pass, transmembrane domain proteins which include IL-8RA, IL-8RB and fusin (also designated LESTR or CXCR-4). Fusin is highly homologous to the IL-8 receptors, sharing 37% sequence identity at the amino acid level. The IL-8 receptors bind to IL-8, NAP-2 and MSGA, while fusin binds to its cognate ligand, SDF-1. Fusin has been identified as the major co-receptor for T-tropic HIV-1, and SDF-1 has been shown to inhibit HIV-1 infection. Six human SDF-1 isoforms exist due to alternative splicing of CXCL12, the gene encoding SDF-1. Three isoforms are known for mouse and rat.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

Publications for CXCL12/SDF-1 ELISA Kit (NBP3-11823)(1)

Publication using NBP3-11823

• Gui M, Huang J, Sheng H et al. High-Dose Vitamin C Alleviates Pancreatic Necrosis by Inhibiting Platelet Activation Through the CXCL12/CXCR4 Pathway in Severe Acute Pancreatitis Journal of inflammation research 2023-07-10 [PMID: 37456783] (ELISA)

FAQs for CXCL12/SDF-1 ELISA Kit (NBP3-11823). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: CXCL12