CXCL12/SDF-1 Antibody

Western Blot: CXCL12/SDF-1 Antibody [NBP2-89133] - Analysis of SDF1 on Hela cell lysate using anti-SDF1 antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: CXCL12/SDF-1 Antibody [NBP2-89133] - Analysis of paraffin-embedded human placenta tissue using anti-SDF1 antibody. Counter stained with hematoxylin.

Flow Cytometry: CXCL12/SDF-1 Antibody [NBP2-89133] - Analysis of HepG2 cells with SDF1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.

Immunohistochemistry-Paraffin: CXCL12/SDF-1 Antibody [NBP2-89133] - Analysis of paraffin-embedded human colon cancer tissue using anti-SDF1 antibody. Counter stained with hematoxylin.

Product Discontinued

CXCL12/SDF-1 Antibody Summary

• Immunogen: Recombinant protein within human CXCL12/SDF-1 aa 22-93. (SwissProt: P48061 Human)
• Localization: Secreted.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CXCL12
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500
• Theoretical MW: 10 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH7.4), 0.2% BSA, 50% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for CXCL12/SDF-1 Antibody

• chemokine (C-X-C motif) ligand 12
• C-X-C motif chemokine 12
• CXCL12
• hIRH
• hSDF-1
• Intercrine reduced in hepatomas
• IRH
• PBSF
• PBSFSDF-1a
• Pre-B cell growth-stimulating factor
• SCYB12
• SDF1
• SDF-1
• SDF1A
• SDF1B
• SDF1SDF-1b
• stromal cell-derived factor 1
• TLSF
• TLSF-a
• TLSF-b
• TPAR1

Background

The C-X-C or alpha chemokine family is characterized by a pair of cysteine residues separated by a single amino acid and primarily functions as chemoattractants for neutrophils. The C-X-C family includes IL-8, NAP-2, MSGA and stromal cell-derived factor-1, or SDF-1. SDF-1 was originally described as a pre-B cell stimulatory factor, but has now been shown to function as a potent chemoattractant for T cells and monocytes, but not neutrophils. Receptors for the C-X-C family are G protein-coupled, seven-pass, transmembrane domain proteins which include IL-8RA, IL-8RB and fusin (also designated LESTR or CXCR-4). Fusin is highly homologous to the IL-8 receptors, sharing 37% sequence identity at the amino acid level. The IL-8 receptors bind to IL-8, NAP-2 and MSGA, while fusin binds to its cognate ligand, SDF-1. Fusin has been identified as the major co-receptor for T-tropic HIV-1, and SDF-1 has been shown to inhibit HIV-1 infection. Six human SDF-1 isoforms exist due to alternative splicing of CXCL12, the gene encoding SDF-1. Three isoforms are known for mouse and rat.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: CXCL12