CXCL12/SDF-1 Antibody [Biotin]

• Reactivity: Hu, Mu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

CXCL12/SDF-1 Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant human CXCL12/SDF‑1 alpha (R&D Systems, Catalog # 350-NS)
  Lys22-Lys89
  Accession # Q6ICW0
• Specificity: Detects human and mouse CXCL12/SDF-1 in ELISAs and Western blots. In sandwich immunoassays, approximately 20% cross-reactivity with recombinant human (rh) SDF-1 beta is observed and less than 0.1% cross-reactivity with rhIP‑10 and recombinant mouse CRG‑2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: CXCL12
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Human/Mouse CXCL12/SDF-1 Antibody (Catalog # MAB350)
  ELISA Detection: Human/Mouse CXCL12/SDF-1 Biotinylated Antibody (Catalog # BAF310)
  Standard: Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF-1 alpha (Catalog # 350-NS)
  Standard: Recombinant Mouse CXCL12/SDF-1 alpha (Catalog # 460-SD)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL12/SDF-1 Antibody [Biotin]

• chemokine (C-X-C motif) ligand 12
• C-X-C motif chemokine 12
• CXCL12
• hIRH
• hSDF-1
• Intercrine reduced in hepatomas
• IRH
• PBSF
• PBSFSDF-1a
• Pre-B cell growth-stimulating factor
• SCYB12
• SDF1
• SDF-1
• SDF1A
• SDF1B
• SDF1SDF-1b
• stromal cell-derived factor 1
• TLSF
• TLSF-a
• TLSF-b
• TPAR1

Background

SDF-1 alpha and SDF-1 beta are the first cytokines initially identified using the signal sequence trap cloning strategy from a mouse bone-marrow stromal cell line. These proteins were subsequently also cloned from a human stromal cell line as cytokines that supported the proliferation of a stromal cell-dependent pre-B-cell line. SDF-1 alpha  and SDF-1 beta cDNAs encode precursor proteins of 89 and 93 amino acid residues, respectively. SDF-1 alpha and SDF-1 beta are encoded by a single gene and arise by alternative splicing. The two proteins are identical except for the four amino acid residues that are present in the carboxy-terminus of SDF-1 beta and absent from SDF-1 alpha . The amino acid sequence of SDF-1/PBSF identified the protein to be a member of the chemokine alpha subfamily that lacks the ELR domain. Unlike other known chemokine alpha and beta subfamily members that cluster on chromosomes 4 and 17, respectively, SDF-1/PBSF was mapped to chromosome 10q11.1. SDF-1/PBSF is highly conserved between species, with only one amino acid substitution between the mature human and mouse proteins. SDF-1/PBSF has been found to be a chemoattractant for T-lymphocytes and monocytes, but not neutrophils. SDF-1/PBSF was shown to be a ligand for CXCR4 (fusin/LESTR) receptor that functions as a coreceptor for lymphocyte-tropic HIV-1 strains. SDF-1/PBSF has been found to be a powerful inhibitor of infection by lymphocyte-tropic HIV-1 strains.

1. Tashiro, K. et al. (1993) Science 261:600.
2. Bleul, C. et al. (1996) Nature 382:829.
3. Oberlin, E. et al. (1996) Nature 382:833.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL12/SDF-1 Antibody (BAF310)(18)

Publications using BAF310

• C Fielding, A García-Gar, C Korn, S Gadomski, Z Fang, JL Reguera, JA Pérez-Simó, B Göttgens, S Méndez-Fer Cholinergic signals preserve haematopoietic stem cell quiescence during regenerative haematopoiesis Nature Communications, 2022-01-27;13(1):543. 2022-01-27 [PMID: 35087060] (ELISA Detection, Mouse)
• Z Benet, Z Jing, DR Fooksman Plasma cell dynamics in the bone marrow niche Cell Reports, 2021-02-09;34(6):108733. 2021-02-09 [PMID: 33567286] (ELISA Capture, Mouse)
• A Venkatesh, H Nandigam, M Muccioli, M Singh, T Loftus, D Lewis, M Pate, F Benencia Regulation of inflammatory factors by double-stranded RNA receptors in breast cancer cells Immunobiology, 2017-11-22;0(0):. 2017-11-22 [PMID: 29331323] (ELISA Development (Detection), Mouse)
• Pratibha Singh, Jonathan Hoggatt, Malgorzata M. Kamocka, Khalid S. Mohammad, Mary R. Saunders, Hongge Li et al. Neuropeptide Y regulates a vascular gateway for hematopoietic stem and progenitor cells Journal of Clinical Investigation 11/13/2017 [PMID: 29130940]
• DI Bromage, S Taferner, M Pillai, DM Yellon, SM Davidson A novel recombinant antibody specific to full-length stromal derived factor-1 for potential application in biomarker studies PLoS ONE, 2017-04-05;12(4):e0174447. 2017-04-05 [PMID: 28379992] (ELISA Development (Detection), Rat)
• N Guidi, M Sacma, L Ständker, K Soller, G Marka, K Eiwen, JM Weiss, F Kirchhoff, T Weil, JA Cancelas, MC Florian, H Geiger Osteopontin attenuates aging-associated phenotypes of hematopoietic stem cells EMBO J, 2017-03-02;0(0):. 2017-03-02 [PMID: 28254837] (ELISA Development (Detection), Mouse)
• H Pierce, D Zhang, C Magnon, D Lucas, JR Christin, M Huggins, GJ Schwartz, PS Frenette Cholinergic Signals from the CNS Regulate G-CSF-Mediated HSC Mobilization from Bone Marrow via a Glucocorticoid Signaling Relay Cell Stem Cell, 2017-02-09;0(0):. 2017-02-09 [PMID: 28196601] (ELISA Development (Detection), Mouse)
• Roy , Ishan, Zimmerman , Noah P, Mackinnon , A Craig, Tsai , Susan, Evans , Douglas, Dwinell , Michael CXCL12 chemokine expression suppresses human pancreatic cancer growth and metastasis. PLoS ONE, 2014-03-04;9(3):e90400. 2014-03-04 [PMID: 24594697] (ELISA Development (Detection), Human)
• Baptista A, Olivier B, Goverse G, Greuter M, Knippenberg M, Kusser K, Domingues R, Veiga-Fernandes H, Luster A, Lugering A, Randall T, Cupedo T, Mebius R Colonic patch and colonic SILT development are independent and differentially regulated events. Mucosal Immunol, 2012-09-19;6(3):511-21. 2012-09-19 [PMID: 22990625] (IHC, Mouse)
• Andrew Chow, Daniel Lucas, Andrés Hidalgo, Simón Méndez-Ferrer, Daigo Hashimoto, Christoph Scheiermann et al. Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell niche Journal of Experimental Medicine 2/14/2011 [PMID: 21282381]
• Won Sik Lee, Yasuhiro Suzuki, Scott S. Graves, Mineo Iwata, G.M. Venkataraman, Marco Mielcarek et al. Canine Bone Marrow Derived Mesenchymal Stromal Cells Suppress Allo-Reactive Lymphocyte Proliferation in Vitro but Fail to Enhance Engraftment in Canine Bone Marrow Transplantation Biology of Blood and Marrow Transplantation 4/1/2011 [PMID: 20457265]
• Williams CK, Segarra M, Sierra Mde L, Sainson RC, Tosato G, Harris AL Regulation of CXCR4 by the Notch ligand delta-like 4 in endothelial cells. Cancer Res., 2008-03-15;68(6):1889-95. 2008-03-15 [PMID: 18339870] (Western Blot, Human)
• Grivel JC, Elliott J, Lisco A, Biancotto A, Condack C, Shattock RJ, McGowan I, Margolis L, Anton P HIV-1 pathogenesis differs in rectosigmoid and tonsillar tissues infected ex vivo with CCR5- and CXCR4-tropic HIV-1. AIDS, 2007;21(10):1263-72. 2007 [PMID: 17545702] (Luminex Development, Human)
• Lisignoli G, Cristino S, Piacentini A, Cavallo C, Caplan AI, Facchini A Hyaluronan-based polymer scaffold modulates the expression of inflammatory and degradative factors in mesenchymal stem cells: Involvement of Cd44 and Cd54. J. Cell. Physiol., 2006-05-01;207(2):364-73. 2006-05-01 [PMID: 16331675] (ELISA Development, Human)
• Deiva K, Geeraerts T, Salim H, Leclerc P, Hery C, Hugel B, Freyssinet JM, Tardieu M Fractalkine reduces N-methyl-d-aspartate-induced calcium flux and apoptosis in human neurons through extracellular signal-regulated kinase activation. Eur. J. Neurosci., 2004-12-01;20(12):3222-32. 2004-12-01 [PMID: 15610155] (ICC, IHC-Fr, Human)
• Yoshihiro Ueda, Kaiyong Yang, Sandra J. Foster, Motonari Kondo, Garnett Kelsoe Inflammation Controls B Lymphopoiesis by Regulating Chemokine CXCL12 Expression The Journal of Experimental Medicine 1/5/2004 [PMID: 14707114]
• Yoshihiro Ueda, Kaiyong Yang, Sandra J. Foster, Motonari Kondo, Garnett Kelsoe Inflammation Controls B Lymphopoiesis by Regulating Chemokine CXCL12 Expression The Journal of Experimental Medicine 2004-01-05 [PMID: 14707114] (ELISA Detection, Mouse, Transgenic Mouse)
• Villalba S, Salvucci O, Aoki Y, De La Luz Sierra M, Gupta G, Davis D, Wyvill K, Little R, Yarchoan R, Tosato G Serum inactivation contributes to the failure of stromal-derived factor-1 to block HIV-I infection in vivo. J. Leukoc. Biol., 2003-07-22;74(5):880-8. 2003-07-22 [PMID: 12960279] (ELISA Development, Western Blot, Human)

Review for CXCL12/SDF-1 Antibody (BAF310) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer ELISA Human 01/12/2015

Summary

• Application: ELISA
• Sample Tested: See PMID 22287708
• Species: Human

Bioinformatics

• Gene Symbol: CXCL12
• Uniprot:
  • Q6ICW0()