CXCL12/SDF-1 Antibody [Unconjugated]

Images

 
Recombinant Human/Feline/Rhesus Macaque CXCL12/SDF‑1 alpha (Catalog # 350-NS) chemoattracts the BaF3 mouse pro‑B cell line transfected with human CXCR4 in a dose-dependent manner (orange line). The amount of cells ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, B/N
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Conjugate Size Price
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Catalog# & Formulation Size Price

CXCL12/SDF-1 Antibody [Unconjugated] Summary

Immunogen
E. coli-derived recombinant human CXCL12/SDF‑1 alpha
Lys22-Lys89
Accession # Q6ICW0
Specificity
Detects human CXCL12/SDF-1 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
CXCL12
Purity Statement
Antigen Affinity-purified
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • Neutralization 1.5-6.0 ug/mL
Reviewed Applications
Read 2 Reviews rated 4
using
AF-310-NA in the following applications:

Publications
Read Publications using
AF-310-NA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for CXCL12/SDF-1 Antibody [Unconjugated]

  • chemokine (C-X-C motif) ligand 12
  • C-X-C motif chemokine 12
  • CXCL12
  • hIRH
  • hSDF-1
  • Intercrine reduced in hepatomas
  • IRH
  • PBSF
  • PBSFSDF-1a
  • Pre-B cell growth-stimulating factor
  • SCYB12
  • SDF1
  • SDF-1
  • SDF1A
  • SDF1B
  • SDF1SDF-1b
  • stromal cell-derived factor 1
  • TLSF
  • TLSF-a
  • TLSF-b
  • TPAR1

Background

CXCL12, also known as SDF-1 (stromal cell-derived factor 1) and PBSF (Pre-B cell growth stimulatory factor), is a member of the CXC chemokine superfamily. Two alternatively spliced mRNAs encode CXCL12 alpha (89 aa residues) and CXCL12 beta (93 aa residues) that differ by the addition of 4 amino acids at the carboxy-terminus. Human and mouse CXCL12 are highly conserved. Mature human and mouse CXCL12 differ only by 1 amino acid.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for CXCL12/SDF-1 Antibody (AF-310-NA)(16)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 8 applications: Blocking/Neutralizing, ELISA Development (Capture), Functional Assay, ICC, IHC, IHC-P, Neutralization, Western Blot.


Filter By Application
Blocking/Neutralizing
(1)
ELISA Development (Capture)
(1)
Functional Assay
(1)
ICC
(3)
IHC
(1)
IHC-P
(1)
Neutralization
(5)
Western Blot
(2)
All Applications
Filter By Species
Human
(14)
Mouse
(1)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using AF-310-NA Applications Species
V Rolfes, LS Ribeiro, I Hawwari, L Böttcher, N Rosero, S Maasewerd, MLS Santos, T Próchnicki, CMS Silva, CWS Wanderley, M Rothe, SV Schmidt, HJ Stunden, D Bertheloot, MN Rivas, CJ Fontes, LH Carvalho, FQ Cunha, E Latz, M Arditi, BS Franklin Platelets Fuel the Inflammasome Activation of Innate Immune Cells Cell Rep, 2020;31(6):107615. 2020 [PMID: 32402278] (Blocking/Neutralizing, Human) Blocking/Neutralizing Human
JL Viñas, M Spence, A Gutsol, W Knoll, D Burger, J Zimpelmann, DS Allan, KD Burns Receptor-Ligand Interaction Mediates Targeting of Endothelial Colony Forming Cell-derived Exosomes to the Kidney after Ischemic Injury Sci Rep, 2018;8(1):16320. 2018 [PMID: 30397255] (Neutralization, Human) Neutralization Human
Hanes M, Salanga C, Chowdry A, Comerford I, McColl S, Kufareva I, Handel T Dual targeting of the chemokine receptors CXCR4 and ACKR3 with novel engineered chemokines. J Biol Chem, 2015;290(37):22385-97. 2015 [PMID: 26216880] (ELISA Development (Capture), Human) ELISA Development (Capture) Human
Boudot A, Kerdivel G, Lecomte S, Flouriot G, Desille M, Godey F, Leveque J, Tas P, Le Drean Y, Pakdel F COUP-TFI modifies CXCL12 and CXCR4 expression by activating EGF signaling and stimulates breast cancer cell migration. BMC Cancer, 2014;14(0):407. 2014 [PMID: 24906407] (Western Blot, Human) Western Blot Human
Richter R, Jochheim-Richter A, Ciuculescu F, Kollar K, Seifried E, Forssmann U, Verzijl D, Smit M, Blanchet X, von Hundelshausen P, Weber C, Forssmann W, Henschler R Identification and characterization of circulating variants of CXCL12 from human plasma: effects on chemotaxis and mobilization of hematopoietic stem and progenitor cells. Stem Cells Dev, 2014;23(16):1959-74. 2014 [PMID: 24708339] (Functional Assay, Human) Functional Assay Human
Yin X, Johns S, Kim D, Mikulski Z, Salanga C, Handel T, Macal M, Zuniga E, Fuster M Lymphatic specific disruption in the fine structure of heparan sulfate inhibits dendritic cell traffic and functional T cell responses in the lymph node. J Immunol, 2014;192(5):2133-42. 2014 [PMID: 24493818]
Ruiz E, Oeztuerk-Winder F, Ventura J A paracrine network regulates the cross-talk between human lung stem cells and the stroma. Nat Commun, 2014;5(0):3175. 2014 [PMID: 24430801] (Western Blot, Human) Western Blot Human
Ouwehand K, Santegoets SJ, Bruynzeel DP, Scheper RJ, de Gruijl TD, Gibbs S CXCL12 is essential for migration of activated Langerhans cells from epidermis to dermis. Eur. J. Immunol., 2008;38(11):3050-9. 2008 [PMID: 18924211] (Neutralization, Human) Neutralization Human
Brzoska E, Grabowska I, Hoser G, Streminska W, Wasilewska D, Machaj EK, Pojda Z, Moraczewski J, Kawiak J Participation of stem cells from human cord blood in skeletal muscle regeneration of SCID mice. Exp. Hematol., 2006;34(9):1262-70. 2006 [PMID: 16939819] (IHC, Human) IHC Human
Carman CV, Springer TA A transmigratory cup in leukocyte diapedesis both through individual vascular endothelial cells and between them. J. Cell Biol., 2004;167(2):377-88. 2004 [PMID: 15504916] (ICC, Human) ICC Human
Show All 16 Publications.

Reviews for CXCL12/SDF-1 Antibody (AF-310-NA) (2) 42

Average Rating: 4
(Based on 2 reviews)
We have 2 reviews tested in 2 species: Human, Other.

Reviews using AF-310-NA:
Filter by Applications
IP
(2)
All Applications
Filter by Species
Human
(1)
Other
(1)
All Species
Images Ratings Applications Species Date Details
  4
reviewed by:
Anonymous
IP Human 01/07/2015
View

Summary

ApplicationImmunoprecipitation
Sample TestedSee PMID 23009808
SpeciesHuman

Blocking

Blocking DetailsSee PMID 23009808

Details

Detection NotesSee PMID 23009808

Comments

CommentsPublished: See PMID 23009808Independent review from antibodyresource.com
  4
reviewed by:
Anonymous
IP Other 01/07/2015
View

Summary

ApplicationImmunoprecipitation
Sample TestedSee PMID 23009808
SpeciesOther

Blocking

Blocking DetailsSee PMID 23009808

Details

Detection NotesSee PMID 23009808

Comments

CommentsPublished: See PMID 23009808Independent review from antibodyresource.com

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for CXCL12/SDF-1 Antibody (AF-310-NA) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Biotin BAF310

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Bioinformatics Tool for CXCL12/SDF-1 Antibody (AF-310-NA)

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Recent Reviews

4
5
0
4
2
3
0
2
0
1
0

 
Anonymous
01/07/2015
Application: IP
Species: Human

 
Anonymous
01/07/2015
Application: IP
Species: Other

Bioinformatics

Gene Symbol CXCL12
Uniprot