CXCL12/SDF-1 alpha Antibody - Azide and BSA Free

Immunohistochemistry-Paraffin: CXCL12/SDF-1 alpha Antibody [NBP2-29480] - Formalin fixed paraffin embedded tissue section of human colorectal carcinoma stained using SDF-1 alpha antibody (NBP2-29480) with DAB detection. Sodium citrate buffer (pH6.0) based heat mediated antigen retrieval was employed in this IHC-P validation.

Immunohistochemistry-Paraffin: CXCL12/SDF-1 alpha Antibody [NBP2-29480] - Formalin fixed paraffin embedded tissue section of human colorectal carcinoma stained using SDF-1 alpha antibody (NBP2-29480) with DAB detection. Sodium citrate buffer (pH6.0) based heat mediated antigen retrieval was employed in this IHC-P validation.

• Reactivity: Hu
• Applications: WB, ELISA, Func, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

CXCL12/SDF-1 alpha Antibody - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit CXCL12/SDF-1 alpha Antibody - Azide and BSA Free (NBP2-29480) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-CXCL12/SDF-1 alpha Antibody: Cited in 4 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant human SDF-1alpha
• Specificity: NBP2-29480 recognises human SDF 1 alpha, otherwise known as CXCL12a, a 68 amino acid stromal cell derived CXC chemokine, which arises from alternative splicing of the SDF 1 gene. The two isoforms, SDF 1 alpha and SDF 1 beta, share an identical amino acid sequence, except for an additional four residues in the C terminal region of the beta isoform.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CXCL12
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 0.5-2.0ug/ml
  • Functional 2.0-4.0ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5ug/ml
  • Western Blot 0.1-0.2ug/ml
• Application Notes: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections.Sodium citrate buffer pH 6.0 is recommended for this purpose. NBP2-29480 may be used under either reducing or non reducing western blotting conditions. Use in Immunohistochemistry reported in scientific literature (PMID: 31116013).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Reconstitute with 0.1 ml distilled water. Add 0.09% Sodium Azide for long term storage.

Alternate Names for CXCL12/SDF-1 alpha Antibody - Azide and BSA Free

• CXCL12 chemokine (C-X-C motif) ligand 12
• CXCL12/SDF-1 alpha
• IRH
• PBSF
• SCYB12
• SDF1 alpha
• SDF1
• SDF1a
• SDF1B
• TLSF
• TPAR1

Background

SDF 1 binds with high affinity to the G protein coupled receptor CXCR4 (fusin) and acts as a chemoattractant for T and B lymphocytes, monocytes and migratory neurons, and is a vital factor in haematopoiesis and angiogenesis. By competitively binding to CXCR4, SDF 1 acts as an inhibitor for the CXCR4 mediated entry of HIV 1 virus into target T cells, and the SDF 1 CXCR4 interaction is important for the regulation of trafficking of normal and malignant stem cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for CXCL12/SDF-1 alpha Antibody (NBP2-29480)(5)

Publications using NBP2-29480

• Jing R, Chen Y, Xu M et al. Investigating the mechanism of Gentiopicroside in rheumatoid arthritis through network pharmacology, molecular docking, and experimental validation. Scientific reports 2025-06-06 [PMID: 40473698]
• Ammar HI, Shamseldeen AM, Shoukry HS et al. Metformin impairs homing ability and efficacy of mesenchymal stem cells for cardiac repair in streptozotocin-induced diabetic cardiomyopathy in rats American journal of physiology. Heart and circulatory physiology 2021-01-29 [PMID: 33513084] (IF/IHC)
• Abdelaal NH, Elhefnawy NG, Abdulmonem SR et al. Evaluation of the expression of the stromal cell-derived factor-1 alpha (CXCL 12) in psoriatic patients after treatment with Methotrexate J Cosmet Dermatol 2019-05-22 [PMID: 31116013] (IF/IHC, Human)
• Cheng Y, Luo R, Zheng H et al. Synergistic anti-tumor efficacy of sorafenib and fluvastatin in hepatocellular carcinoma. Oncotarget 2017-04-04 [PMID: 28423574] (IHC-P, Human)
• Nistala R, Habibi J, Aroor A et al. DPP4 Inhibition attenuates filtration barrier injury and oxidant stress in the zucker obese rat. Obesity (Silver Spring) 2014-07-04 [PMID: 24995775] (IHC-P, Rat)
  
  Details:
  SDF1 alpha antibody used for IHC-P on renal/kidney cortical tissues of Zucker Obese (ZO) and age-matched Zucker Lean (ZL; controls) rats. Figure 1E shows the localization of SDF-1 alpha to the glomeruli/distal tubules and its low expression was found in glomerules in the ZO compared vs ZL controls, a difference which was abolished with DPP4 inhibition in this model.

Bioinformatics

• Gene Symbol: CXCL12
• Uniprot:
  • P48061()